Evaluation of phosphate rock as the only source of phosphorus for the growth of tall and semi-dwarf durum wheat and rye plants using digital phenotyping

Abstract

Background Phosphorus nutrition is important for obtaining high yields of crop plants. However, wheat plants are known to be almost incapable of taking up phosphorus from insoluble phosphate sources, and reduced height genes are supposed to decrease this ability further. Methods We performed a pot experiment using Triticum durum Desf. tall spring variety LD222, its near-isogenic semidwarf line carrying Rht17 ( Reduced height 17 ) gene, and winter rye ( Secale cereale L.) variety Chulpan. The individual plants were grown in quartz sand. The phosphorus was provided either as phosphate rock powder mixed with sand, or as monopotassium phosphate solution (normal nutrition control) or was not supplemented at all (no-phosphorus control). Other nutrients were provided in soluble form. During experiment the plants were assessed using the TraitFinder (Phenospex Ltd., Heerlen, Netherlands) digital phenotyping system for a standard set of parameters. Double scan with 90 degrees turns of pots around vertical axis vs . single scan were compared for accuracy of phenotyping. Results The phenotyping showed that at least 20 days of growth after seedling emergence were necessary to get stable differences between genotypes. After this initial period, phenotyping confirmed poor ability of wheat to grow on substrate with phosphate rock as the only source of phosphorus compared to rye; however, Rht17 did not cause an additional reduction in growth parameters other than plant height under this variant of substrate. The agreement between digital phenotyping and conventionally measured traits was at previously reported level for grasses (R 2 = 0.85 and 0.88 for digital biomass and 3D leaf area vs . conventionally measured biomass and leaf area, single scan). Among vegetation indices, only the normalized differential vegetation index (NDVI) and the green leaf index (GLI) showed significant correlations with manually measured traits, including the percentage of dead leaves area. The double scan improved phenotyping accuracy, but not substantially.

Plant phenotyping relevance

TraitFinderデジタルフェノタイピングを用いた形質取得と、スキャン方式の精度比較・従来測定との妥当性検証が明示されており、フェノタイピング手法が技術的に実質的な役割を果たしている。

Code and data availability

The paper's phenotyping data are provided only as a Supplemental File (Data S1) with no public deposit or URL; the sole allowed URL is the Phenospex vendor documentation for plant parameters, which is a generic external resource, not a paper-specific dataset, code, or model asset.

No evidence-backed public reproduction asset is currently recorded.