In situ NIR-IIb imaging of endogenous H 2 S signaling for high-resolution abiotic stress visualization in plants.

Abstract

In vivo analysis of plant stress responses remains a major challenge in precision agriculture, limiting dynamic optimization of crop growth under variable environmental conditions. Fluorescence imaging enables nondestructive tracking of stress biomarkers, but its accuracy is compromised by the low abundance of endogenous signaling molecules, tissue autofluorescence interference, and limited signal penetration depth. Here, we develop a ratiometric near-infrared IIb fluorescent probe for sensitive monitoring of endogenous hydrogen sulfide (H 2 S). This nanoprobe integrates lanthanide nanoparticles with H 2 S-responsive molecular units, enabling enhanced tissue penetration and high-resolution imaging through an absorption competition-induced emission mechanism. Under abiotic stress conditions, the probe visualizes stress-induced fluctuations of H 2 S in living plants. This work establishes an H 2 S-centered strategy for plant stress visualization and provides a foundation for developing early diagnosis platforms.

Plant phenotyping relevance

植物の非生物的ストレス状態を、生体内H₂Sシグナルの蛍光画像として可視化する新規NIR-IIbプローブを開発しており、表現型取得法が研究の中心である。

Code and data availability

The supplied blocks describe a fluorescent nanoprobe study for H2S stress imaging. No public phenotype/trait datasets, plant image collections, author analysis code, or trained models with explicit availability statements and URLs are present. BioRender links are schematic illustrations, not phenotyping assets; 'Source

No evidence-backed public reproduction asset is currently recorded.