Artificial pre-harvest sprouting chamber with moderate humidity better simulates field sprouting for soft winter wheat.

Abstract

Pre-harvest sprouting, germination of the seed on the spike, causes reduced grain quality and marketability in US wheat. Methods devised to induce and study pre-harvest sprouting vary significantly from one another in procedure, induction time, and/or measurement, and often fail to accurately reflect natural sprouting, which varies over years and locations. An artificial sprouting chamber and protocol with a shorter exposure time and relative humidity more relevant to field conditions was significantly correlated ( p p = 0.001) with those subjected to overhead irrigated field sprouting tests over three years. Four pairs of the ten varieties were genetically related but showed significantly different sprouting and alpha amylase activity. These varietal pairs may prove useful in studying the genetics of pre-harvest sprouting. While the study was performed in wheat, other susceptible crops such as rice, barley, rye, and sorghum could be tested using this method.

Plant phenotyping relevance

コムギの穂発芽という植物状態を測定する人工チャンバーとプロトコルを開発し、圃場試験との相関で妥当性を検証しており、表現型取得法が研究の中心である。

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