A turn-on NIR fluorescent probe for risk-assessing oxidative stress in cabbage roots under abiotic stress.

Abstract

Oxidative stress is closely related to the crop health status under stress conditions. H 2 O 2 is an important signaling molecule in plants under stress. Therefore, monitoring H 2 O 2 fluctuations is of great significance when risk-assessing oxidative stress. However, few fluorescent probes have been reported for the in situ tracking of H 2 O 2 fluctuations in crops. Herein, we designed a "turn-on" NIR fluorescent probe (DRP-B) to detect and in situ-image H 2 O 2 in living cells and crops. DRP-B exhibited good detection performance for H 2 O 2 and could image endogenous H 2 O 2 in living cells. More importantly, it could semi-quantitatively visualize H 2 O 2 in cabbage roots under abiotic stress. Visualization of H 2 O 2 in cabbage roots revealed H 2 O 2 upregulation in response to adverse environments (metals, flood, and drought). This study provides a new method for risk-assessing oxidative stress in plants under abiotic stress and is expected to provide guidance for the development of new antioxidant defense strategies to enhance plant resistance and crop productivity.

Plant phenotyping relevance

植物の酸化ストレス状態をH2O2のin situ蛍光イメージングで可視化する新規プローブを開発しており、植物状態の取得法が研究の中心である。

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