Sandwich freezing and freeze substitution of Arabidopsis plant tissues for electron microscopy

Abstract

Abstract Sandwich freezing is a method of rapid freezing by sandwiching specimens between two copper disks, and it has been used for observing exquisite close-to-native ultrastructure of living yeast and bacteria. Recently, this method has been found to be useful for preserving cell images of glutaraldehyde-fixed cultured cells, as well as animal and human tissues. In the present study, this method was applied to observe the fine structure of living Arabidopsis plant tissues and was found to achieve excellent ultrastructural preservation of cells and tissues. This is the first report of applying the sandwich freezing method to observe plant tissues.

Plant phenotyping relevance

植物組織の細胞・組織微細構造を保存・観察するためのサンドイッチ凍結法を植物へ適用した技術研究であり、表現型取得法が中心である。

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