Quantifying the Contribution to Virulence of Phytophthora infestans Effectors in Potato.

Abstract

Late blight in potato, caused by the oomycete Phytophthora infestans, is a devastating disease that significantly impacts potato production. For a proper understanding of disease development, it is important to understand the interaction between plant and pathogen at a molecular level. Like other pathogens, P. infestans secretes effector molecules, which can be recognized by receptors in the plant and trigger immunity. In addition, effectors from P. infestans have been identified to enhance disease development. Here, we describe an assay to investigate the role of effectors in virulence of P. infestans on potato. We combine agroinfiltration to transiently express effectors in potato with detached leaf assays to monitor disease development. This protocol makes it possible to conveniently quantify the effect of individual effectors on virulence of P. infestans. The identification of effectors with an important role in late blight development can help to design better strategies to control the disease.

Plant phenotyping relevance

ジャガイモ葉の病害進展を測定し、病原性への寄与を定量するアッセイ自体を中心的に記述しているため、植物病害表現型の測定法開発として収録する。

Code and data availability

This is a methods protocol chapter (agroinfiltration plus detached leaf assay) with no public phenotype datasets, images, code, models, or supplements deposited. The only URLs mentioned are a generic vector catalog (gatewayvectors.vib.be) for the pK7WG2 plasmid and a commercial supplier site (koppert.nl) for Thripex; a

No evidence-backed public reproduction asset is currently recorded.