Unverified paper record
Serial Block Electron Microscopy to Study Plasmodesmata in the Vasculature of Arabidopsis thaliana Roots.
Methods in molecular biology (Clifton, N.J.) · 1 Jan 2022 · 10.1007/978-1-0716-2132-5_5
Abstract
Serial block electron microscopy (SB-EM) is a technique that enables acquisition and reconstruction of 3D cellular volumes. The approach is valuable for the study of plasmodesmata (PD) as the relative positions of these structures are contained in the datasets. In this chapter, we describe how to prepare plant roots for SB-EM via fixation, embedding, and trimming steps. We also provide details and recommendations for later image acquisition and processing. The procedure is suitable to work on root vascular tissues.
Plant phenotyping relevance
植物根の細胞構造を3D画像化・再構成する手順と画像取得・処理を中心に扱っており、植物組織の形態状態を抽出するイメージング手法として方法論的に該当する。
abstractSerial block electron microscopy (SB-EM) is a technique that enables acquisition and reconstruction of 3D cellular volumes.
abstractIn this chapter, we describe how to prepare plant roots for SB-EM via fixation, embedding, and trimming steps. We also provide details and recommendations for later image acquisition and processing.
Code and data availability
公開論文であることは確認できましたが、現在の公式API・許可済み取得経路では本文を自動取得できませんでした。
No evidence-backed public reproduction asset is currently recorded.
This is an automatically classified, unverified record. Curator approval is required before any resource enters the Catalog.