Real-Time Lineage Analysis to Study Cell Division Orientation in the Arabidopsis Shoot Meristem.

Abstract

Cells in the Arabidopsis shoot apical meristem are small and divide frequently throughout the life-time of the organism making them good candidates for studying the mechanisms of cell division in plants. But tracking these cell divisions requires multiple images to be taken of the same specimen over time which means the specimen must stay alive throughout the process. This chapter provides details on how to prepare plants for live imaging, keep them alive and growing through multiple time points, and how to process the data to extract cell boundary coordinates from three-dimensional images.

Plant phenotyping relevance

生体を維持した多時点3Dライブイメージングと画像処理による細胞境界座標抽出が中心で、細胞分裂の向き・系譜という植物組織の形態状態を定量化する方法を扱う。

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