Immunofluorescence Detection of Callose in Plant Tissue Sections.

Abstract

The accumulation of the cell wall component callose at plasmodesmata (PD) is crucial for the regulation of symplastic intercellular transport in plants. Here we describe protocols to fluorescently image callose in sectioned plant tissue using monoclonal antibodies. This protocol achieves high-resolution images by the fixation, embedding, and sectioning of plant material to expose internal cell walls. By using this protocol in combination with high-resolution confocal microscopy, we can detect PD callose in a variety of plant tissues and species.

Plant phenotyping relevance

植物組織内のPD calloseという細胞状態を蛍光・共焦点画像で取得するプロトコル自体が中心であり、単なる生物学的実験の routine 測定ではない。

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