Development of an assessment key and techniques for field screening of tea (Camellia sinensis L.) cultivars for resistance to blister blight

Abstract

Blister blight caused by the biotrophic fungus, Exobasidium vexans Massee, is the most problematic foliar disease of tea in Sri Lanka. A reliable and accurate method is needed for field assessment of severity of the disease for epidemiological studies, formulating disease control strategies and crop improvement programmes. A field assessment key with 0–6 scores was developed for blister blight, considering the lowest (0) and highest (>30%) limits of disease severity observed in the field and different stages of symptom development. The key was validated by six raters, 3 experienced and 3 inexperienced. The field assessment trials made using the key were accurate and precise (R2 > 0.80). The area under the disease progress curve (AUDPC), calculated using the disease severity levels obtained using the assessment key, was used to combine multiple observations of disease progress into a 0–9 susceptible scale. Ascending numbers in the scale represent increasing susceptibility. The new scale was proposed to discriminate blister blight resistance in tea accessions/cultivars in field screening. Screening trials for validation of the susceptible scale, conducted using tea cultivars of known resistance or susceptibility levels and newly developed accessions of tea, at three locations, revealed that the 0–9 scale is simple to apply, offers a fine discrimination of blister blight resistance levels, and allows objective evaluation.

Plant phenotyping relevance

茶の葉の病害重症度を定量評価するフィールド評価キーと抵抗性スケールを開発・検証しており、植物表現型の取得手法が研究の中心です。

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