High-resolution imaging of plant delayed luminescence

Abstract

Delayed luminescence (DL) is a quantized signal that is characteristic of photoexcited molecules entering a relaxed state. Studying DL provides critical insight into photophysical mechanisms through the analysis of specific spatiotemporal dynamics. In this study, we developed a high-sensitivity DL imaging system using a quantitative scientific complementary metal-oxide-semiconductor (qCMOS) camera and a single-photon counting resolution. By optimizing the optical architecture and signal processing algorithms together, we achieved full-field spatiotemporal DL imaging at megapixel resolution (i.e., 2304 × 4096 pixels). Key findings include the following: (1) we observed spatial heterogeneity in DL intensity across the leaves of Arabidopsis thaliana, with stronger signals detected in veins and at sites of mechanical injury; (2) Species-specific DL responses occur in response to oxidative stress, with Hydrocotyle vulgaris and Ginkgo biloba showing enhanced central DL activity; (3) excitation using white light induced maximum DL intensity, while red and blue light differentially modulated decay kinetics. Finally, we develop a two-level quantum model that links DL dynamics to the populations of excited-state electrons, thereby developing a theoretical framework for future photophysical research. Collectively, this work establishes a theoretical and technological framework for advancing plant phenotyping under stress conditions and optimizing light environments.

Plant phenotyping relevance

植物の遅延発光を高感度・高解像度で取得する画像システムと信号処理を開発しており、ストレス下の植物生理状態を測定するフェノタイピング手法が中心である。

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