Extending Hyperspectral Imaging for Plant Phenotyping to the UV-Range

Abstract

Previous plant phenotyping studies have focused on the visible (VIS, 400–700 nm), near-infrared (NIR, 700–1000 nm) and short-wave infrared (SWIR, 1000–2500 nm) range. The ultraviolet range (UV, 200–380 nm) has not yet been used in plant phenotyping even though a number of plant molecules like flavones and phenol feature absorption maxima in this range. In this study an imaging UV line scanner in the range of 250–430 nm is introduced to investigate crop plants for plant phenotyping. Observing plants in the UV-range can provide information about important changes of plant substances. To record reliable and reproducible time series results, measurement conditions were defined that exclude phototoxic effects of UV-illumination in the plant tissue. The measurement quality of the UV-camera has been assessed by comparing it to a non-imaging UV-spectrometer by measuring six different plant-based substances. Given the findings of these preliminary studies, an experiment has been defined and performed monitoring the stress response of barley leaves to salt stress. The aim was to visualize the effects of abiotic stress within the UV-range to provide new insights into the stress response of plants. Our study demonstrated the first use of a hyperspectral sensor in the UV-range for stress detection in plant phenotyping.

Plant phenotyping relevance

UV hyperspectralイメージングセンサーを植物フェノタイピング向けに導入・評価し、再現性のある測定条件と塩ストレス検出を示しており、表現型取得法が研究の中心である。

Code and data availability

The article describes UV hyperspectral imaging of barley salt stress and substance measurements, but contains no data availability statement, no public dataset or image deposit, and no author code release. Analysis was done in ENVI 5.5, R/RStudio, and SigmaPlot without any shared scripts or URLs. The only URL present (

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