Rapid and non-destructive screening of seed components in domesticated pennycress using near-infrared spectroscopy

Abstract

Domesticated pennycress (Thlaspi arvense L.), a newly winter annual oilseed crop grown in the Upper Midwestern USA, has garnered significant interest because of its high seed oil content. Compared to native field pennycress, domesticated varieties possess improved agronomic and compositional properties that allow grain and meal to be used as an animal feed ingredient. Near infrared spectroscopy (NIRS) is a well-established, non-destructive method for rapidly analyzing seed composition, however NIRS has been challenging to utilize in native field pennycress because of the limited natural variation in the seed composition does not allow the development of robust NIRS calibration equations. Domesticated pennycress exhibits notable differences in certain seed composition components and using this variation we observed moderate to strong correlations between the wet lab analyses and NIRS predictions for fourteen traits evaluated. For moisture, crude fat, protein, and sinigrin content, coefficient of determination (r²) between wet lab values and the NIRS predictions were 0.98, 0.96, 0.97 and 0.94 respectively. For fatty acid content, moderate r² for oleic acid (0.87), linoleic acid (0.69), linolenic acid (0.88) and erucic acid (0.87) were observed. Additionally, a comparison of grain samples harvested in 2024 was made for results from the NIRS equations and standard laboratory methods executed by external analytical laboratories. These results collectively demonstrate that NIRS is an effective tool for compositional analysis of seed components of domesticated pennycress and can serve as a substitute for more time-consuming analytical methods.

Plant phenotyping relevance

近赤外分光法（NIRS）による種子成分推定を湿式分析および外部検査法と比較・検証しており、種子形質の取得手法が研究の中心である。

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