Closed-chamber hydroponics for whole-plant phenotyping v1

Abstract

Noninvasive phenotyping can quantify dynamic plant growth processes at higher temporal resolution than destructive phenotyping and can reveal phenomena that would be missed by end-point analysis alone. Additionally, whole-plant phenotyping can identify growth conditions that are optimal for both above- and below-ground tissues. However, noninvasive, whole-plant phenotyping approaches available today are generally expensive, complex, and non-modular. We developed a low-cost and versatile approach to non-invasively measure whole-plant physiology over time by growing plants in isolated hydroponic chambers. We demonstrate the versatility of our approach by measuring whole-plant biomass accumulation, water consumption, and water use efficiency every two days on unstressed and osmotically-stressed sorghum cultivars. Our system can be implemented using cheap, basic components and requires no specific technical expertise. It can, in theory, be used for any non-aquatic vascular plant species.

Plant phenotyping relevance

低コストで非破壊的に全植物の生理・成長形質を経時測定する水耕チャンバー手法を開発しており、フェノタイピング手法が研究の中心である。

Code and data availability

The supplied document is a protocols.io protocol describing a closed-chamber hydroponics phenotyping method. It contains no public phenotype/trait datasets, plant images, sensor/3D inputs, author analysis code, trained models, or supplements with such assets. The only external link is the companion bioRxiv preprint, ac

No evidence-backed public reproduction asset is currently recorded.