Development of predictive models for total phenolics and free p-coumaric acid contents in barley grain by near-infrared spectroscopy.

Abstract

Barley grains are rich in phenolic compounds, which are associated with reduced risk of chronic diseases. Development of barley cultivars with high phenolic acid content has become one of the main objectives in breeding programs. A rapid and accurate method for measuring phenolic compounds would be helpful for crop breeding. We developed predictive models for both total phenolics (TPC) and p-coumaric acid (PA), based on near-infrared spectroscopy (NIRS) analysis. Regressions of partial least squares (PLS) and least squares support vector machine (LS-SVM) were compared for improving the models, and Monte Carlo-Uninformative Variable Elimination (MC-UVE) was applied to select informative wavelengths. The optimal calibration models generated high coefficients of correlation (r pre ) and ratio performance deviation (RPD) for TPC and PA. These results indicated the models are suitable for rapid determination of phenolic compounds in barley grains.

Plant phenotyping relevance

大麦穀粒のフェノール含量という育種対象の植物形質を、NIRSと予測モデルで迅速測定する手法を開発・比較し、モデル性能を評価しているため、化学分析の単なる routine 測定ではなく表現型取得法が中心である。

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