Detection of roll rotation of naturally occurring crystals inside plant leaves and detection of adhesivity of the interior surface of leaf cell

Abstract

A rigid body can have 3 degrees of rotational freedom. Of these, the yaw or in-plane rotation is well known and studied while the pitch or the first in-plane rotation is somewhat studied. However the roll or the second in-plane rotation has not been so well studied. It is here that we show how to detect roll rotation for a 4-fold symmetric Calcium Oxalate crystal inside a plant leaf cell by using the anisotropy of the scatter pattern while trapped in optical tweezers, behind a set of crossed polarizers. The difference in halves signal in the appropriate sense gives roll rotation while that in another sense gives pitch rotation of the crystal. We show that this can be used to perform nano-tribology of the interior surface of the leaf cell with a contact radius of about 500nm, without relying upon Atomic Force Microscopes, thus enabling soft probing.

Plant phenotyping relevance

植物葉細胞内の結晶回転を光トラップと散乱異方性で検出し、葉細胞内面の接着性を定量する新規測定法が中心であり、植物細胞の生理的状態を取得する方法開発に該当する。

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