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Pre-harvest screening on-vine of spinach quality and safety using NIRS technology.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy · 19 Sept 2018 · 10.1016/j.saa.2018.09.035

Abstract

The study sought to perform a non-destructive and in-situ quality evaluation of spinach plants using near infrared (NIR) spectroscopy in order to establish its suitability for different uses once harvested. Modified partial least square (MPLS) regression models using NIR spectra of intact spinach leaves were developed for nitrate, ascorbic acid and soluble solid contents. The residual predictive deviation (RPD) values were 1.29, 1.21 and 2.54 for nitrate, ascorbic acid and soluble solid contents, respectively. Later, this predictive capacity increased for nitrate content (RPDcv = 1.63) when new models were developed, taking into account the influence on the robustness of the model exercised by the simultaneity between the NIR and laboratory analyses. Subsequently, using partial least squares discriminant analysis (PLS-DA), the ability of NIRS technology to classify spinach as a function of nitrate content was tested. PLS-DA yielded percentages of correctly classified samples ranging from 73.08-76.92% for the class 'spinach able to be used fresh' to 85.71-73.08% for the class 'preserved, deep-frozen or frozen spinach, both for unbalanced and balanced models respectively, based on NH signal associated with proteins. Overall, the data supports the capability of NIR spectroscopy to establish the final destination of the production of spinach analysed on the plant, as a screening tool for important safety and quality parameters.

Plant phenotyping relevance

NIR分光を用いた生体 spinach の非破壊・現場品質評価法を開発し、回帰モデルの予測性能と分類性能を検証しているため、植物形質取得法が研究の中心である。

abstractThe study sought to perform a non-destructive and in-situ quality evaluation of spinach plants using near infrared (NIR) spectroscopy
abstractModified partial least square (MPLS) regression models using NIR spectra of intact spinach leaves were developed
abstractOverall, the data supports the capability of NIR spectroscopy to establish the final destination of the production of spinach analysed on the plant, as a screening tool for important safety and quality parameters.

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