Image-based 3D canopy reconstruction to determine potential productivity in complex multi-species crop systems

Abstract

Background and aims Intercropping systems contain two or more species simultaneously in close proximity. Due to contrasting features of the component crops, quantification of the light environment and photosynthetic productivity is extremely difficult. However it is an essential component of productivity. Here, a low-tech but high-resolution method is presented that can be applied to single- and multi-species cropping systems to facilitate characterization of the light environment. Different row layouts of an intercrop consisting of Bambara groundnut ( Vigna subterranea ) and proso millet ( Panicum miliaceum ) have been used as an example and the new opportunities presented by this approach have been analysed. Methods Three-dimensional plant reconstruction, based on stereo cameras, combined with ray tracing was implemented to explore the light environment within the Bambara groundnut-proso millet intercropping system and associated monocrops. Gas exchange data were used to predict the total carbon gain of each component crop. Key results The shading influence of the tall proso millet on the shorter Bambara groundnut results in a reduction in total canopy light interception and carbon gain. However, the increased leaf area index (LAI) of proso millet, higher photosynthetic potential due to the C4 pathway and sub-optimal photosynthetic acclimation of Bambara groundnut to shade means that increasing the number of rows of millet will lead to greater light interception and carbon gain per unit ground area, despite Bambara groundnut intercepting more light per unit leaf area. Conclusions Three-dimensional reconstruction combined with ray tracing provides a novel, accurate method of exploring the light environment within an intercrop that does not require difficult measurements of light interception and data-intensive manual reconstruction, especially for such systems with inherently high spatial possibilities. It provides new opportunities for calculating potential productivity within multi-species cropping systems, enables the quantification of dynamic physiological differences between crops grown as monoculture and those within intercrops, and enables the prediction of new productive combinations of previously untested crops.

Plant phenotyping relevance

ステレオカメラによる3次元植物再構成とレイトレーシングを開発・適用し、作物キャノピーの光環境や生産性を定量化する手法が研究の中心である。

Code and data availability

The supplied blocks describe stereo-image 3D canopy reconstruction, ray tracing, gas exchange, and Mathematica modelling for a Bambara groundnut–proso millet intercrop, but contain no public deposit, availability statement, or authors' URL for phenotype datasets, images, reconstructions, or analysis code. Only a CC-BY

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