Synchrotron-based DEI and DEI-CT systems to image chick pea seeds, plant anatomy and the associated physiology at 30 keV.

Abstract

Summary The study the effect of contrast on seeds, growth and the associated anatomy and physiology, with upgraded imaging systems. The use of phase information to explore new information at various stage of the growth. This work benefits, the use of Synchrotron-based DEI and DEI-CT systems to enhance the contrast in plant root architecture and contrast mechanisms, visibility of fine structures of root architecture growth and some aspects of physiology at acceptable level. These non-destructive, imaging systems available at the X-15A beamline, at NSLS, BNL, USA, are utilized. Noticed detailed anatomical and physiological observations, contrast mechanisms, with these upgraded systems, compared to other conventional techniques, equipped with tube source of X-rays. Examined the potential of these systems to quantify the plant roots in situ. The acquired images provided good contrast, anatomical structures and physiology of the plant root micro-architecture. We observed some of the complex plant traits, such as growth, development, root architecture and the associated physiology. The interior structure, root architecture, root morphology, growth of laterals and subsequent laterals can be visualized directly by synchrotron-based imaging techniques. Root architecture of the plant grown from seeds provides new information about the structure and enhancement of some desired property, for example, interior micro-structure of the root laterals and the subsequent laterals and the clear visibility of the leaves in detail. This way, it will be possible to differentiate the weakly and strongly attenuation of the signal traversing within the sample, clearly reflects the acceptable visibility in root laterals, subsequent laterals and the associated opaque matrix with enhanced contrast. The sample has a thin layer of hard structure outside and protein inside. Extinction properties of these samples will be characterized by Sy-DEI and Sy-DEI-CT. This way, we may be able to differentiate softly and weakly attenuation within the sample, to know more about the contrast mechanisms. The visibility, contrast and porosity, with finer details, can be noticed, with Sy-DEI-CT systems as distinguished from Sy-DEI. However, limited field of view, may limit the problems associated with Sy-DEI-CT.

Plant phenotyping relevance

シンクロトロンX線DEI/DEI-CTによる植物根の解剖構造・根系形態・成長・生理の非破壊画像化と定量化可能性が研究の中心であり、植物フェノタイピング手法に該当する。

Code and data availability

The paper describes synchrotron DEI/DEI-CT imaging of chick pea seeds and root architecture, but contains no public phenotype dataset, image deposit, author code release, or supplement with such assets. Custom IDL programs are mentioned but no availability statement or URL is given. The only URL (plant-image-analysis)

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