Identification of plant leaf phosphorus content at different growth stages based on hyperspectral reflectance

Abstract

Abstract Background: Modern agriculture strives to sustainably manage fertilizer for both economic and environmental reasons. The monitoring of any nutritional (phosphorus, nitrogen, potassium) deficiency in growing plants is a challenge for precision farming technology. A study was carried out on three species of popular crops, celery ( Apium graveolens L., cv. Neon), sugar beet ( Beta vulgaris L., cv. Tapir) and strawberry ( Fragaria × ananassa Duchesne, cv. Honeoye), fertilized with four different doses of phosphorus (P) to deliver data for non-invasive detection of P content. Results: Data obtained via biochemical analysis of the chlorophyll and carotenoid contents in plant material showed that the strongest effect of P availability for plants was in the diverse total chlorophyll content in sugar beet and celery compared to that in strawberry, in which P affects a variety of carotenoid contents in leaves. The measurements performed using hyperspectral imaging, obtained in several different stages of plant development, were applied in a supervised classification experiment. A machine learning algorithm (Backpropagation Neural Network, Random Forest, Naive Bayes and Support Vector Machine) was developed to classify plants from four variants of P fertilization. The lowest prediction accuracy was obtained for the earliest measured stage of plant development. Statistical analyses showed correlations between leaf biochemical constituents, phosphorus fertilization and the mass of the leaf/roots of the plants. Conclusions: Obtained results demonstrate that hyperspectral imaging combined with artificial intelligence methods has potential for non-invasive detection of non-homogenous phosphorus fertilization on crop levels.

Plant phenotyping relevance

植物葉のリン栄養状態を非侵襲的に推定するため、ハイパースペクトル画像と機械学習分類器を中心的に開発・適用しており、植物フェノタイピング手法に該当する。

Code and data availability

The supplied blocks describe hyperspectral phenotyping of celery, sugar beet, and strawberry under four phosphorus treatments, but contain no public dataset, image, code, model, or supplement deposit. No data or code availability statement with an authors' public URL appears; only commercial software (ENVI, The Unscram

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