A Luciferase Imaging-Based Assay for Studying Temperature Compensation of the Circadian Clock.

Abstract

The pace of circadian rhythms remains relatively unchanged across a physiologically relevant range of temperatures, a phenomenon known as temperature compensation. Temperature compensation is a defining characteristic of circadian rhythms, ensuring that clock-regulated processes occur at approximately the same time of day across a wide range of conditions. Despite the identification of several genes involved in the regulation of temperature compensation, the molecular mechanisms underlying this process are still not well understood. High-throughput assays of circadian period are essential for the investigation of temperature compensation. In this chapter, we present a luciferase imaging-based method that enables robust and accurate examination of temperature compensation in the plant circadian clock.

Plant phenotyping relevance

植物の概日時計の周期を高スループットかつ高精度に測定するルシフェラーゼ画像化法を提示しており、植物生理状態の取得手法が中心である。

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