Imaging of Potassium and Calcium Distribution in Plant Tissues and Cells to Monitor Stress Response and Programmed Cell Death.

Abstract

In plants, the response to stress, such as salinity, pathogen attack, drought, high concentration of metals, hyperthermia, and hypothermia, is usually accompanied by potassium ion (K + ) leakage from the cytosol to the cell wall, mediated by plasma membrane cation conductivity. Stress-induced electrolyte leakage co-occurs with accumulation of reactive oxygen species (ROS) and calcium ions (Ca 2+ ) and often results in programmed cell death (PCD). The development of X-ray and mass spectrometry (MS) based imaging techniques has enabled insight into the spatial tissue and cell-specific redistribution of major and trace elements during the stress response. In this chapter a workflow for sample preparation, imaging, and image analysis by X-ray and MS based techniques is presented.

Plant phenotyping relevance

植物組織・細胞のストレス応答や細胞死に伴うイオン分布を可視化するX線/MS画像化について、試料調製・画像取得・画像解析のワークフローを中心に扱っており、植物状態の計測手法が中核です。

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