Quantitative and dynamic cell polarity tracking in plant cells

Abstract

Summary Quantitative information on the spatiotemporal distribution of polarised proteins is central for understanding cell‐fate determination, yet collecting sufficient data for statistical analysis is difficult to accomplish with manual measurements. Here we present Polarity Measurement (P ome ), a semi‐automated pipeline for the quantification of cell polarity and demonstrate its application to a variety of developmental contexts. P ome analysis reveals that, during asymmetric cell divisions in the Arabidopsis thaliana stomatal lineage, polarity proteins BASL and BRXL2 are more asynchronous and less mutually dependent than previously thought. A similar analysis of the linearly arrayed stomatal lineage of Brachypodium distachyon revealed that the MAPKKK BdYDA1 is segregated and polarised following asymmetrical divisions. Our results demonstrate that P ome is a versatile tool, which by itself or combined with tissue‐level studies and advanced microscopy techniques can help to uncover new mechanisms of cell polarity.

Plant phenotyping relevance

植物細胞の極性を定量化する半自動パイプラインを開発・適用しており、植物の細胞状態を抽出する方法が研究の中心です。

Code and data availability

The paper describes the Pome pipeline (Fiji macro + R script) and its Supporting Information Methods S1/Notes S1-S2 user guide, but no public deposit or authors' URL for the code, data, or images is provided in the supplied blocks. The only URLs present are ORCID profiles, license links, and generic CRAN/R project URLs

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