Nondestructive Detection and Quantification of Dysprosium in Plant Tissues

Abstract

ABSTRACT Background The growing demand for rare-earth elements (REEs), particularly dysprosium (Dy), in part driven by clean energy technologies, underscores the need for sustainable extraction methods. Recovery of Dy, particularly from geographically distributed waste sources is challenging. This gap positions phytomining—a technique using plants to accumulate metals— as a promising alternative. However, plant species differ in their ability to accumulate metals in high concentrations, necessitating efficient screening methods. In this study, we developed a high-throughput fluorescence-based assay to detect and quantify Dy uptake in plant tissues. Results Our Dy detection method exploits Dy’s unique spectroscopic properties for sensitive and efficient analysis, enabling detection of concentrations as low as 0.3 µM. By incorporating sodium tungstate (Na WO) as a fluorescence enhancer, we achieved robust emissions at 480 and 580 nm, facilitating Dy quantification in complex plant matrices. Additionally, time-resolved fluorescence techniques reduced background autofluorescence from plant tissues, enhancing signal specificity. Validation against Inductively Coupled Plasma Mass Spectrometry (ICP-MS) demonstrated strong correlation. Greenhouse trials confirmed the method’s utility for screening Dy accumulation in living plants and highlight the potential for rapid standoff detection. Conclusions This fluorescence-based approach offers a scalable, efficient tool for identifying Dy-accumulating plants, advancing phytomining as a sustainable strategy for REE recovery.

Plant phenotyping relevance

植物組織中のDy蓄積という植物状態を対象に、蛍光による高スループット測定法を開発し、ICP-MSで検証しているため、フェノタイピング手法が中心である。

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