High-resolution synchrotron imaging studies of intact fresh roots reveal soil bacteria promoted bioremediation and bio-fortification

Abstract

Plant-microbe interactions can be utilized in bio-based processes such as bioremediation and biofortification, either to remove hazardous radionuclides and heavy metals from the soil, or to increase the accumulation of desired elements into crops to improve their quality. Optimizing such elegant plant-microbe interactions requires detailed understanding of the chemical element compositions of fresh plant tissues at cellular organelle resolution. However, such analyses remain challenging because conventional methods lack the required spatial resolution, contrast or sensitivity. Using a novel combination of nanoscaleresolution 3D cryogenic synchrotron-light ptychography, holotomography and fluorescence tomography, we show how soil bacteria interact with Arabidopsis thaliana and promote the uptake of various metals. Co-cultivation with Pseudomonas sp. strain T5-6-I alters root anatomy and increases levels of selenium (Se), iron (Fe) and other micronutrients in roots. Our approach highlights the interaction of plants and microbes in bioremediation and biofortification on the subcellular level.

Plant phenotyping relevance

植物組織の元素分布・根形態を高分解能で取得する新規イメージング手法が研究の中心であり、植物状態の定量的解析に直接用いられているため。

Code and data availability

The supplied blocks describe synchrotron PXCT/XNH/XRNF imaging of Arabidopsis roots and reference supplementary material (e.g., 'Se_Fe statistics.xlsx' and 3D XRNF videos 'provided in online material'), but no public repository, dataset deposit, or author-provided URL for these assets appears anywhere in the text. No D

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