Probing the in-situ volumes of Arabidopsis leaf plastids using 3D confocal and scanning electron microscopy

Abstract

Leaf plastids harbor a plethora of biochemical reactions including photosynthesis, one of the most important metabolic pathways on earth. Scientists are eager to unveil the physiological processes within the organelle but also their interconnection with the rest of the plant cell. An increasingly important feature of this venture is to use experimental data in the design of metabolic models. A remaining obstacle has been the limited in situ volume information of plastids and other cell organelles. To fill this gap for chloroplasts, we established three microscopy protocols delivering in situ volumes based on: 1) chlorophyll fluorescence emerging from the thylakoid membrane, 2) a CFP marker embedded in the envelope, and 3) calculations from serial block-face scanning electron microscopy (SBFSEM). The obtained data were corroborated by comparing wild-type data with two mutant lines affected in the plastid division machinery known to produce small and large mesophyll chloroplasts, respectively. Furthermore, we also determined the volume of the much smaller guard cell plastids. Interestingly, their volume is not governed by the same components of the division machinery which defines mesophyll plastid size. Based on our three approaches the average volume of a mature Col-0 wild-type mesophyll chloroplasts is 93 {micro}m3. Wild-type guard cell plastids are approximately 18 {micro}m3. Lastly, our comparative analysis shows that the chlorophyll fluorescence analysis can accurately determine chloroplast volumes, providing an important tool to research groups without access to transgenic marker lines expressing genetically encoded fluorescence proteins or costly SBFSEM equipment. Significance statement -sentence summaryThis work describes and compares three different strategies to obtain accurate volumes of leaf plastids from Arabidopsis, the most widely used model plant. We hope our contribution will support quantitative metabolic flux modeling and spark other projects aimed at a more metric-driven plant cell biology.

Plant phenotyping relevance

葉緑体体積という植物器官・細胞形態形質を取得する3種類の顕微鏡プロトコルを確立・比較し、変異体で検証しているため、表現型取得法が研究の中心です。

Code and data availability

The supplied blocks describe confocal/SBFSEM chloroplast volume measurements and ImageJ/Amira processing, but no public phenotype dataset, image data, author code, or supplement with such assets is stated. The only URL mentioned (Bio-Formats) is a generic third-party library used for file import, not a paper-specific.

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