Focused Ion Beam Milling and Cryo-electron Tomography Methods to Study the Structure of the Primary Cell Wall in Allium cepa .

Abstract

Cryo-electron tomography (cryo-ET) is a formidable technique to observe the inner workings of vitrified cells at a nanometric resolution in near-native conditions and in three-dimensions. One consequent drawback of this technique is the sample thickness, for two reasons: i) achieving proper vitrification of the sample gets increasingly difficult with sample thickness, and ii) cryo-ET relies on transmission electron microscopy (TEM), requiring thin samples for proper electron transmittance ( This protocol was validated in: Curr Biol (2022), DOI: 10.1016/j.cub.2022.04.024.

Plant phenotyping relevance

タマネギ細胞壁の三次元構造を観察するためのFIBミリングとクライオ電子線トモグラフィーの手法・プロトコルが中心であり、植物の形態・構造状態を取得する方法を扱う。

Code and data availability

植物フェノタイピング解析を再現する公開資産であることを、入力本文と直接リンクから確認できなかったため保留しました。

No evidence-backed public reproduction asset is currently recorded.