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Evaluation of LiDAR-based Canopy Trait Estimation in Midwestern Row Crops

bioRxiv (Cold Spring Harbor Laboratory) · 30 Sept 2025 · 10.1101/2025.09.28.678542

Abstract

Abstract Stand count, the number of plants per unit ground area, and leaf area index (LAI), the ratio of leaf area to ground area, are critical traits for crop research but are traditionally measured using labor-intensive methods. While new sensing technologies are being developed, quantifying improvement in measurement efficiency and data quality, relative to traditional techniques, is lacking. In this study, we use LiDAR to generate 3D scans of corn and soybean plots and evaluate two computational methods: a gap fraction approach to estimate LAI and a persistent homology algorithm to estimate stand count by detecting structural peaks in the canopy. Validation experiments and statistical comparisons of bias and variance demonstrate that LiDAR-derived LAI estimates in corn are comparable in quality to those from established instruments. However, in soybean, the LiDAR method performs poorly, likely due to dense canopies limiting light penetration and structural differentiation. Stand count estimations in corn closely match manual counts, with the added benefit of full-plot coverage and significantly faster data collection. In soybean, stand count estimates are unreliable under dense canopy conditions. These results offer practical guidance for the use of LiDAR in field phenotyping and highlight both its current capabilities and limitations. While a trade-off between speed and precision remains, particularly in high-density canopies, LiDAR’s scalability and multi-trait potential make it a promising tool for high-throughput breeding programs. Continued improvements in LiDAR hardware and algorithm design may further enhance measurement accuracy and extend applicability across crops and growth stages.

Plant phenotyping relevance

LiDARによるLAI・立ち株数推定を開発・比較検証し、バイアス、分散、精度、適用限界を評価しており、植物表現型取得法が研究の中心である。

abstractwe use LiDAR to generate 3D scans of corn and soybean plots and evaluate two computational methods: a gap fraction approach to estimate LAI and a persistent homology algorithm to estimate stand count by detecting structural peaks in the canopy.
abstractValidation experiments and statistical comparisons of bias and variance demonstrate that LiDAR-derived LAI estimates in corn are comparable in quality to those from established instruments.

Code and data availability

The supplied blocks describe LiDAR point cloud collection and persistent homology/gap-fraction analyses for stand count and LAI, but contain no data availability statement, no public phenotype dataset or point cloud deposit, and no author code with a public URL. All URLs in the text are literature citations, not paper-

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