New sources of lentil germplasm for aluminium toxicity tolerance identified by high throughput hydroponic screening.

Abstract

Aluminium (Al) toxicity in acid soils inhibits root elongation and development causing reduced water and nutrient uptake by the root system, which ultimately reduces the crop yield. This study established a high throughput hydroponics screening method and identified Al toxicity tolerant accessions from a set of putative acid tolerant lentil accessions. Four-day old lentil seedlings were screened at 5 µM Al (pH 4.5) for three days in hydroponics. Measured pre and post treatment root length was used to calculate the change in root length (ΔRL) and relative root growth (RRG%). A subset of 15 selected accessions were used for acid soil Al screening, and histochemical and biochemical analyses. Al treatment significantly reduced the ΔRL with an average of 32.3% reduction observed compared to the control. Approximately 1/4 of the focused identification of germplasm strategy accessions showed higher RRG% than the known tolerant line ILL6002 which has the RRG% of 37.9. Very tolerant accessions with RRG% of > 52% were observed in 5.4% of the total accessions. A selection index calculated based on all root traits in acid soil screening was highest in AGG70137 (636.7) whereas it was lowest in Precoz (76.3). All histochemical and biochemical analyses supported the hydroponic results as Northfield, AGG70137, AGG70561 and AGG70281 showed consistent good performance. The identified new sources of Al tolerant lentil germplasm can be used to breed new Al toxicity tolerant lentil varieties. The established high throughput hydroponic method can be routinely used for screening lentil breeding populations for Al toxicity tolerance. Future recommendations could include evaluation of the yield potential of the selected subset of accessions under acid soil field conditions, and the screening of a wider range of landrace accessions originating from areas with Al toxic acid soils.

Plant phenotyping relevance

アルミニウム耐性 germplasm の同定が主目的だが、高スループット水耕スクリーニング法の確立と、酸性土壌・組織化学・生化学による検証が明示され、根の成長を定量する再利用可能な表現型取得法が中心的に扱われている。

Code and data availability

The article describes hydroponic and soil screening of lentil accessions for aluminium tolerance, with data in Online Resources, but no public phenotype dataset, image/sensor inputs, author analysis code, or trained model is deposited or linked. The only URLs present (FAOSTAT, MCPD descriptors, NLWRA soil report) are a

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