High-throughput phenotyping reveals differential transpiration behavior within the banana wild relatives highlighting diversity in drought tolerance

Abstract

Crop wild relatives, the closely related species of crops, may harbor potentially important sources of new allelic diversity for (a)biotic tolerance or resistance. However, to date wild diversity is only poorly characterized and evaluated. Banana has a large wild diversity but only a narrow proportion is currently used in breeding programs. The main objective of this work was to evaluate genotype-dependent transpiration responses in relation to the environment. By applying continuous high-throughput phenotyping, we were able to construct genotype-specific transpiration response models in relation to light, VPD and soil water potential. We characterized and evaluated 6 (sub)species and discerned four phenotypic clusters. Significant differences were observed in leaf area, cumulative transpiration and transpiration efficiency. We confirmed a general stomatal-driven ‘isohydric’ drought avoidance behavior, but discovered genotypic differences in the onset and intensity of stomatal closure. We pinpointed crucial genotype specific environmental conditions when drought avoidance mechanisms were initiated and when stress kicked in. Differences between (sub)species were more pronounced under certain environmental conditions, illustrating the need for high-throughput dynamic phenotyping, modelling and validation. We conclude that the banana wild relatives contain useful drought tolerance traits, emphasizing the importance of their conservation and potential for use in breeding programs.

Plant phenotyping relevance

連続的なハイスループット表現型計測とモデル構築により、遺伝型特異的な蒸散応答や乾燥耐性形質を抽出・検証しており、フェノタイピング手法が研究の中心である。

Code and data availability

The supplied blocks contain only the title page and abstract. The sole hosted file is the manuscript document itself (Eyland_EPPN_version4.docx), which is the article, not a paper-specific dataset, image, code, or model asset. No data or code availability statement, repository, identifier, or public URL for phenotyping

No evidence-backed public reproduction asset is currently recorded.