Low-Cost Continuous-Wave Diffusive Microtomography with Fiber-Scanned White-Light Illumination

Abstract

Tomographic microscopy enables three-dimensional internal imaging but often requires expensive optical or X-ray instrumentation. Here we present an ultra-low-cost continuous-wave diffusive tomography (CWDT) system for biological samples. The system uses a smartphone microscope, a white LED coupled into an optical fiber, 3D-printed micropositioners, and a physics-based forward model optimized with machine learning. We demonstrate full-color volumetric reconstructions from a tartrazine-cleared poplar section, a scattering phantom, fungal mycelium near an Arabidopsis root, and thick poplar branch imaging with an inserted side-emitting fiber. The current results are qualitative and exploratory, but they show that scanned fiber illumination and inexpensive hardware can produce useful three-dimensional reconstruction outputs for low-cost microscopy experiments.

Plant phenotyping relevance

低コスト三次元断層イメージング法そのものを開発し、ポプラ組織・枝やシロイヌナズナ根近傍を対象に植物の内部構造を可視化しているため、植物形態の取得法として中心的です。

Code and data availability

The paper's raw imaging inputs, configurations, and reconstruction outputs for Figures 2–5 are publicly deposited on Kaggle. The analysis code repository is only 'prepared for release' (no confirmed public deposit yet), so it is listed as request-only. Hardware CAD mirrors are public but are instrument designs, not the