Confocal Live Imaging of Reproductive Organs Development in Arabidopsis .

Abstract

Understanding how multicellular organisms are shaped requires high-resolution, quantitative data to unravel how biological structures grow and develop over time. In recent years, confocal live imaging has become an essential tool providing insights into developmental dynamics at cellular resolution in plant organs such as leaves or meristems. In the context of flowers, growth tracking has primarily been limited to sepals, the outermost floral organs, or the post-fertilization gynoecium, which are easily accessible for microscopy. Here, we describe a detailed pipeline for the preparation, dissection, and confocal imaging of the development of internal reproductive floral organs of Arabidopsis thaliana including both the stamen and gynoecium. We also discuss how to acquire high-quality images suitable for efficient 2D and 3D segmentation that allow the quantification of cellular dynamics underlying their development. Key features • Fine dissection of tiny and tightly enclosed floral organs. • Confocal live imaging method allowing long-term observation of plant reproductive morphogenesis. • Assessing the quality of acquired images for efficient segmentation at cellular resolution in 2D and 3D.

Plant phenotyping relevance

植物の生殖器官を対象とした共焦点ライブイメージングと、2D/3Dセグメンテーションによる細胞動態定量化の実験・解析パイプラインが中心であり、植物表現型取得法の開発に該当する。

Code and data availability

This is a methods/protocol article describing confocal live imaging of Arabidopsis reproductive organs. No public phenotype datasets, author-deposited images, analysis code, or trained models are mentioned. MorphoGraphX and its website/forum are generic third-party analysis tools, not paper-specific assets, and no data

No evidence-backed public reproduction asset is currently recorded.