Estimation of Stomatal Aperture in Arabidopsis thaliana Using Silicone Rubber Imprints.

Abstract

Estimation of stomatal aperture using low viscosity silicone-base impression material has the advantage of working with the whole leaf. The developmental stage and the environment strongly affect the stomatal aperture. Therefore, it is mandatory to have accurate estimations of the stomatal aperture of intact leaves under different situations. With this technique, it is possible to get the real picture at any moment. The outputs of the data include studies on cell area and morphology, epidermis cell and stomata lineages, among others. This protocol is useful for the accurate estimation of stomatal aperture in many samples of intact leaves in Arabidopsis thaliana .

Plant phenotyping relevance

シリコーンゴム印象法により、無傷葉の気孔開度を正確に推定する実験プロトコルであり、植物生理形質の取得方法自体が中心である。

Code and data availability

This protocol paper describes silicone-imprint stomatal aperture measurement using ImageJ, but provides no public dataset, images, author code, or model deposit. ImageJ is a generic third-party tool, not a paper-specific asset.

No evidence-backed public reproduction asset is currently recorded.