Quantifying Plant Growth and Cell Proliferation with MorphoGraphX.

Abstract

Confocal microscopy is widely used to live-image plant tissue. Cell outlines can be visualized using fluorescent probes that mark the cell wall or plasma membrane, enabling the confocal microscope to be used as a 3D scanner with submicron precision. After imaging, the data needs to be analyzed by specialized software to quantify the features of interest, such as cell size and shape, growth rates and anisotropy, and gene expression. Here we present a protocol for the 3D image processing software MorphoGraphX ( www.MorphoGraphX.org ) using time-lapse images of an Arabidopsis thaliana sepal and the shoot apex of tomato.

Plant phenotyping relevance

MorphoGraphXによる3D画像処理と時系列画像解析が中心で、植物細胞のサイズ・形状・成長率などの表現型を定量化するプロトコルを提示している。

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