Unverified paper record
Detection of Reactive Oxygen Species (ROS) Levels in the Fruitlet Abscission Zone of Litchi.
Methods in molecular biology (Clifton, N.J.) · 1 Jan 2025 · 10.1007/978-1-0716-4470-6_7
Abstract
Reactive oxygen species (ROS) are pivotal in regulating plant organ abscission. The buildup of ROS in the fruitlet abscission zone (FAZ) actively triggers the abscission of litchi fruitlets. In this chapter, we present a simple method to measure intracellular ROS levels in the FAZ of litchi using 2,7-dichlorodi-hydrofluorescein diacetate (DCFH-DA). Litchi FAZ samples are transverse sectioned and then incubated with a 50 μM DCFH-DA solution at room temperature for 1 h. DCF fluorescence can be visualized using a laser scanning confocal microscope, and the fluorescence intensity is then analyzed with ImageJ software.
Plant phenotyping relevance
リュウガン果実の離層における植物のROS状態を、蛍光イメージングと画像解析で測定する実験プロトコルが中心であり、植物生理フェノタイピング手法に該当する。
abstractwe present a simple method to measure intracellular ROS levels in the FAZ of litchi using 2,7-dichlorodi-hydrofluorescein diacetate (DCFH-DA).
abstractDCF fluorescence can be visualized using a laser scanning confocal microscope, and the fluorescence intensity is then analyzed with ImageJ software.
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