al development in arid regions. ORCID Sultan Md Monwarul Islam http://orcid.org/0009-0002-7219-2104 Izzat Sidahmed Ali Tahir http://orcid.org/0000-0002-1711-6961 Kinya Akashi http://orcid.org/0000-0002-9991-5766 Data availability statement The root images of wheat N61 and MSD417 are deposited in the Zenodo data repository under https://doi.org/10.5281/zenodo.18080159 and https://doi.org/10.5281/zenodo.18079748, respectively. The microscopic images of coleorhiza are deposited under https://doi.org/10.5281/zenodo.18091131. The other original contributions presented in the study are included in the article and/or supplementary material. References Alahmad, S., El Hassouni, K., Bassi, F. M., Di
Open resource ↗Zenodo · 10.5281/zenodo.18080159 · pdf-raw-page:14 lines:1-49Unverified paper record
A practical phenotyping framework for root system architecture reveals enhanced root vigor in an Aegilops tauschii -derived wheat line
Cogent Food & Agriculture · 29 Jun 2026 · 10.1080/23311932.2026.2689176
Abstract
Wild-relative introgression broadens wheat diversity, as exemplified by the Multiple Synthetic Derivatives (MSD) population, a unique hexaploid wheat resource capturing extensive genetic diversity from Aegilops tauschii. However, root system architecture (RSA), a key determinant of resource acquisition and stress adaptation, remains poorly characterized in this population. Here, we established a practical two-dimensional root phenotyping framework that enables continuous imaging to track RSA traits and their responses to heat stress. Using this framework we evaluated MSD417 as a representative genotype against its recurrent parent, Norin 61 (N61). Under control conditions, MSD417 displayed greater total root length, root system width, and convex hull area than N61 (p < 0.001), indicating enhanced early root vigor. MSD417 also exhibited larger second pair seminal root angle (p < 0.001) and length (p < 0.01) across both conditions, suggesting enhanced horizontal root exploration while maintaining similar rooting depth to N61 (p = 0.981). Heat stress reduced overall root growth and narrowed genotypic differences, limiting RSA expression. Microscopic observations revealed a lower coleorhiza height-to-width ratio in MSD417. These findings demonstrate the effectiveness of the two-dimensional platform for early-stage RSA phenotyping and highlight Aegilops tauschii-derived germplasm as a source of favorable root traits in wheat breeding.
Plant phenotyping relevance
二次元画像による根系構造フェノタイピング基盤を構築し、連続撮像で根形質を追跡する方法が研究の中心であるため含める。
abstractHere, we established a practical two-dimensional root phenotyping framework that enables continuous imaging to track RSA traits and their responses to heat stress.
abstractThese findings demonstrate the effectiveness of the two-dimensional platform for early-stage RSA phenotyping
Code and data availability
The paper's data availability statement deposits the paper-specific phenotyping inputs publicly on Zenodo: root images of wheat N61 and MSD417 (the two genotypes measured for RSA traits) and microscopic coleorhiza images. These are public, paper-specific image datasets directly underlying the study's measurements. No作者
Md Monwarul Islam http://orcid.org/0009-0002-7219-2104 Izzat Sidahmed Ali Tahir http://orcid.org/0000-0002-1711-6961 Kinya Akashi http://orcid.org/0000-0002-9991-5766 Data availability statement The root images of wheat N61 and MSD417 are deposited in the Zenodo data repository under https://doi.org/10.5281/zenodo.18080159 and https://doi.org/10.5281/zenodo.18079748, respectively. The microscopic images of coleorhiza are deposited under https://doi.org/10.5281/zenodo.18091131. The other original contributions presented in the study are included in the article and/or supplementary material. References Alahmad, S., El Hassouni, K., Bassi, F. M., Dinglasan, E., Youssef, C., Quarry, G., Aksoy,
Open resource ↗Zenodo · 10.5281/zenodo.18079748 · pdf-raw-page:14 lines:1-49-6961 Kinya Akashi http://orcid.org/0000-0002-9991-5766 Data availability statement The root images of wheat N61 and MSD417 are deposited in the Zenodo data repository under https://doi.org/10.5281/zenodo.18080159 and https://doi.org/10.5281/zenodo.18079748, respectively. The microscopic images of coleorhiza are deposited under https://doi.org/10.5281/zenodo.18091131. The other original contributions presented in the study are included in the article and/or supplementary material. References Alahmad, S., El Hassouni, K., Bassi, F. M., Dinglasan, E., Youssef, C., Quarry, G., Aksoy, A., Mazzucotelli, E., Juhász, A., Able, J. A., Christopher, J., Voss-Fels, K. P., & Hickey, L. T. (2019). A maj
Open resource ↗Zenodo · 10.5281/zenodo.18091131 · pdf-raw-page:14 lines:1-49This is an automatically classified, unverified record. Curator approval is required before any resource enters the Catalog.