Validation of Brix for predicting sugar concentrations of alfalfa and orchardgrass

Abstract

Objectives: The objective of this study was to evaluate the accuracy of Brix in predicting sugar concentrations in fresh alfalfa (ALF; Medicago sativa L.) and orchardgrass (OG; Dactylis glomerata L.) forages to be used as an inexpensive and rapid field-level assessment of relative energy in forages. Materials and Methods: In a 2-yr study, fresh forages samples from ALF and OG monoculture pastures in central Pennsylvania, USA, were collected once monthly from May to September. Samples were immediately evaluated for Brix values with a hand-held digital refractometer, and the remaining biomass was frozen immediately with liquid N to halt cellular respiration. Samples were lyophilized and analyzed for individual sugars and wet chemistry nutritive analyses. Results and Discussion: Brix was positively correlated with total and individual sugars in ALF during late spring and late summer (0.53-0.93), but correlations were nonexistent or negative in mid-summer. However, Brix values did not correlate with any notable sugar parameters in OG beyond the first sampling date. These findings were attributed to greater fibrous fraction contamination, lower sugar concentrations found in grasses and legumes compared with horticultural crops, and changes in seasonal growth of ALF and OG. Implications and Applications: Brix values did not consistently predict sugar concentrations in fresh ALF and OG forages. Because Brix measures dissolved solids in solution (not just sugars), Brix readings collected from crushed ALF or OG samples may be confounded by fibrous fractions found in the solution. Brix accuracy may also be dependent on seasonal temperature patterns, plant growth stage, and daily weather patterns. Other solutions should be investigated that rapidly assess sugar profiles and nutritive values of fresh forages.

Plant phenotyping relevance

アルファルファとオーチャードグラスの糖濃度という植物形質を対象に、携帯型屈折計によるBrix測定の予測精度と適用限界を検証しており、測定法の技術評価が研究の中心である。

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