This study compiles and releases the first standardized rapeseed phenology observation dataset spanning forty-four years (1981-2024) over the core winter rapeseed production region of the Middle and Lower Yangtze River Plain in China. The data originate from systematic observations at 50 national-level agrometeorological stations across six provinces: Jiangsu, Zhejiang, Anhui, Jiangxi, Hubei, and Hunan. The dataset provides complete records of the specific dates for each phenology stage from sowing to maturity, including eight key phenology periods: Sowing (SO), Emergence (EM), Five-leaf (FV), Bud Formation (BF), Stem Elongation (SE), Flowering (FL), Green Ripening (GR), and Maturity (MA), along with the calculated durations of six distinct growth lengths. We implemented a multi-level quality control protocol encompassing internal logical checks, statistical outlier detection, climatological validation, time series homogenization, and expert arbitration. This protocol effectively constrained data uncertainty and corrected non-climatic discontinuities. Univariate linear regression was further employed to quantify the decadal change trends of each phenology period and growth length, supplemented by Kernel Density Estimation (KDE) to characterize their probability distribution features. The final dataset is presented as structured tables (in xlsx format) and high-resolution diagnostic plots (including trend and density plots), with a total volume of approximately 470 MB, systematically organized by province and station. This dataset fills a critical gap in long-term, standardized rapeseed phenology data for the region. The integrated analysis of phenology dates, growth stage durations, and their trends across the entire network provides an indispensable, high-quality empirical foundation. It is designed to support in-depth investigations into the nonlinear response mechanisms of overwintering crops to climate warming, improve crop model parameterization and validation, and inform regional adaptive management strategies.
Why it matches plant phenotyping methods44年間のナタネの生育段階日を標準化・品質管理して公開するデータセット研究であり、植物状態（フェノロジー）の測定データ整備が中心です。
abstractThis study compiles and releases the first standardized rapeseed phenology observation dataset spanning forty-four years (1981-2024)
Reproduction assets foundThe paper's rapeseed phenology dataset (1981–2024, 50 stations) is openly deposited in Science Data Bank under DOI 10.57760/sciencedb.34086, containing structured xlsx tables and diagnostic plots. No custom code was created per the authors.Dataset · publicThe dataset described in this work has been deposited in the Science Data Bank (ScienceDB) under
accession code https://doi.org/10.57760/sciencedb.34086 [27].Open asset ↗Science Data Bank · 10.57760/sciencedb.34086pdf-page:12 lines:1-68Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Clubroot disease, caused by Plasmodiophora brassicae , is one of the major constraints in rapeseed production. Breeding disease-resistant cultivars is the best way to control this devastating disease. However, breeding reliable resistant germplasm and genes is limited. Inactivation of susceptible genes has been shown to be a new and effective strategy for developing resistant crops. Therefore, we aimed to screen key candidate susceptible genes in this study. Firstly, we established a stable, high-throughput visualization method for identifying gall formation at the early stage of P.brassicae infection. At 14 days post-inoculation (dpi), the earliest time point with a clear record of scorable root swelling, remarkable variations in the speed of gall formation were observed among 85 genotypes. Secondly, genome-wide association studies (GWAS) were performed to identify genes involved in gall development. Three and two consecutive significant peaks were detected at 14 and 21 dpi, respectively. Thirdly, comparative transcriptomic analysis was conducted between 2AF195 and 2AF058 at 7 and 14 dpi; these two materials exhibit contrasting speeds of gall development. Gene clustering analysis revealed two opposite expression patterns at 14 dpi. One pattern comprised 1,383 genes downregulated in 2AF195 but upregulated in 2AF058, which were significantly enriched in 10 KEGG pathways, including Environmental Information Processing and Plant-pathogen interaction, and involved core repressors JAZ8/10 in the jasmonic acid (JA) signaling pathway, as well as nucleotide-binding site (NBS) protein-encoding genes. The opposite pattern consisted of 79 genes upregulated in 2AF195 but downregulated in 2AF058, which were enriched in an additional 10 KEGG pathways, predominantly related to Carbohydrate Metabolism and the Ubiquitin System. These genes were functionally annotated mainly as pectin methylesterases, xyloglucan endotransglucosylase/hydrolases (XTHs), and lignin biosynthesis-related enzymes. These findings demonstrated that distinct regulatory networks exist in different susceptible rapeseed genotypes. Finally, through the combined analysis of haplotype and transcriptome data, we co-localized and identified the candidate gene BnaC08g46100D , a nodulin-related gene belonging to the MtN21 transporter family. These results provide a theoretical basis for developing novel disease-resistant materials by editing the key susceptibility genes involved in root gall formation. The candidate genes identified in this study are the most promising targets for this purpose.
Why it matches plant phenotyping methods根こぶ形成を高スループットに可視化・判定する方法の確立が明示され、感染植物の病徴を測定する手法として研究の主要な技術要素になっている。
abstractwe established a stable, high-throughput visualization method for identifying gall formation at the early stage of P.brassicae infection.
Reproduction assets found本文中に内容が明示された植物フェノタイピング関連の補足表と、その公開リンクを確認しました。Supplement · publicSupplementary Table 2
Disease incidence data of 85 rapeseed accessions at various time points following inoculation with the Xinmin strain.Open asset ↗lines:502-594Code / dataset availability confirmedCrossref · Europe PMC · OpenAlex · checked 5 Sept 2026
Quantitative characterization of complete canopy architecture is essential for accurate evaluation of crop photosynthesis and yield potential, thereby supporting crop ideotype design. Although various sensing technologies enable three-dimensional (3D) reconstruction of individual plants and canopies, they often fail to describe canopy architecture accurately because of severe occlusion in dense populations. To address this limitation, we developed an effective framework for the 3D reconstruction of complex and dynamic population-scale canopy architecture in rapeseed using unmanned aerial vehicle multi-view imagery combined with a novel point cloud completion model. A complete point cloud generation pipeline was first established to enable automated training data annotation, allowing discrimination between surface points and occluded points within the canopy. The proposed crop population point cloud completion network (CP-PCN) integrates a multi-resolution dynamic graph convolutional encoder, a point pyramid decoder, a dynamic graph convolutional feature extractor, and a generative adversarial network-based loss function to predict occluded canopy points. CP-PCN achieved chamfer distance values of 3.35 to 4.51 cm across four growth stages, outperforming the state-of-the-art transformer-based method PoinTr. Ablation analyses confirmed that each of the four modules contributes to overall model accuracy. In addition, validation experiments showed that the improved architectural completeness achieved by CP-PCN resulted in more accurate yield estimation compared with incomplete and PoinTr-completed point clouds. CP-PCN also demonstrated strong cross-crop generalizability by successfully reconstructing mature rice canopies. Overall, this framework provides a scalable approach for quantitative analysis of complex canopy architectures in field-grown crops.
Why it matches plant phenotyping methodsUAVマルチビュー画像から遮蔽点を補完し、作物群落の3Dキャノピー構造を再構成する手法を開発・検証しており、植物表現型取得が研究の中心です。
abstractwe developed an effective framework for the 3D reconstruction of complex and dynamic population-scale canopy architecture in rapeseed using unmanned aerial vehicle multi-view imagery combined with a novel point cloud completion model
Reproduction assets foundThe paper's Data and code availability statement explicitly deposits all source code and test data for the CP-PCN phenotyping pipeline on a public GitHub repository, matching an allowed URL.Code · publicAll source code and test data used in this study are publicly available on GitHub ( https://github.com/Ziyue-Guo/CP-PCN.git ).Open asset ↗https://github.com/Ziyue-Guo/CP-PCN.git · CP-PCNlines:133-158Code / dataset availability confirmedOpenAlex · arXiv · checked 6 Sept 2026
High resolution phenotyping at the level of individual leaves offers fine-grained insights into plant development and stress responses. However, the full potential of accurate leaf tracking over time remains largely unexplored due to the absence of robust tracking methods-particularly for structurally complex crops such as canola. Existing plant-specific tracking methods are typically limited to small-scale species or rely on constrained imaging conditions. In contrast, generic multi-object tracking (MOT) methods are not designed for dynamic biological scenes. Progress in the development of accurate leaf tracking models has also been hindered by a lack of large-scale datasets captured under realistic conditions. In this work, we introduce CanolaTrack, a new benchmark dataset comprising 5,704 RGB images with 31,840 annotated leaf instances spanning the early growth stages of 184 canola plants. To enable accurate leaf tracking over time, we introduce LeafTrackNet, an efficient framework that combines a YOLOv10-based leaf detector with a MobileNetV3-based embedding network. During inference, leaf identities are maintained over time through an embedding-based memory association strategy. LeafTrackNet outperforms both plant-specific trackers and state-of-the-art MOT baselines, achieving a 9% HOTA improvement on CanolaTrack. With our work we provide a new standard for leaf-level tracking under realistic conditions and we provide CanolaTrack - the largest dataset for leaf tracking in agriculture crops, which will contribute to future research in plant phenotyping. Our code and dataset are publicly available at https://github.com/shl-shawn/LeafTrackNet.
Why it matches plant phenotyping methods葉レベルの時系列追跡という植物表現型取得手法を開発し、専用ベンチマークデータセットで評価しているため、方法が中心である。
abstractTo enable accurate leaf tracking over time, we introduce LeafTrackNet, an efficient framework that combines a YOLOv10-based leaf detector with a MobileNetV3-based embedding network.
Reproduction assets foundThe authors explicitly state that the CanolaTrack dataset (5,704 annotated RGB images of 184 canola plants), the LeafTrackNet code, and trained model weights are publicly available at their GitHub repository.Code · publicOur code and dataset are publicly
available at https://github.com/shl-shawn/LeafTrackNet.Open asset ↗shl-shawn/LeafTrackNet · LeafTrackNetpdf-page:1 lines:1-53Code / dataset availability confirmedOpenAlex · Europe PMC · checked 15 Sept 2026
Rapeseed / canolaNeRF / 3D Gaussian SplattingLiDAR / point cloudFruitCountingSegmentationFruit / seed / panicle traits
Accurate analysis of plant phenotypic traits is crucial for crop breeding and precision agriculture. This study proposes a lightweight semantic segmentation model named KAN-GLNet (Kolmogorov-Arnold Network with Global-Local Feature Modulation), based on an enhanced PointNet++ architecture and integrated with an optimized Density-Based Spatial Clustering of Applications with Noise (DBSCAN) algorithm, to achieve high-precision segmentation and automatic counting of canola siliques. A multi-view point cloud acquisition platform was built, and high-fidelity canola point clouds were reconstructed using Neural Radiance Fields (NeRF) technology. The proposed model includes three key modules: Reverse Bottleneck Kolmogorov-Arnold Network Convolution, a Global-Local Feature Modulation (GLFN) block, and a contrastive learning-based normalization module called ContraNorm. KAN-GLNet contains only 5.72M parameters and achieves 94.50% mIoU, 96.72% mAcc, and 97.77% OAcc in semantic segmentation tasks, outperforming all baseline models. In addition, the DBSCAN workflow was optimized, achieving a counting accuracy of 97.45% in the instance segmentation task. This method achieves an excellent balance between segmentation accuracy and model complexity, providing an efficient solution for high-throughput plant phenotyping. The code and dataset have been made publicly available at: https://anonymous.4open.science/r/KAN-GLNet-6432/.
Why it matches plant phenotyping methodsカノーラ莢のセグメンテーションと自動計数という植物形質抽出手法を、3D点群取得基盤・NeRF再構成・新規モデル・DBSCANワークフローとして開発・評価しており、植物フェノタイピング手法が中心である。
abstractThis study proposes a lightweight semantic segmentation model named KAN-GLNet (Kolmogorov-Arnold Network with Global-Local Feature Modulation), based on an enhanced PointNet++ architecture and integrated with an optimized Density-Based Spatial Clustering of Applications with Noise (DBSCAN) algorithm, to achieve high-precision segmentation and automatic counting of canola siliques.
Reproduction assets foundThe authors explicitly state that their curated code and dataset (canola silique point cloud phenotyping data and KAN-GLNet analysis code) are publicly available at an anonymous.4open.science repository, which is an allowed URL.Code · publicDBSCAN workflow was optimized, achieving a counting accuracy of 97.45% in the instance segmentation task. This method achieves an excellent balance between segmentation accuracy and model complexity, providing an efficient solution for high-throughput plant phenotyping. The code and dataset have been made publicly available at: https://anonymous.4open.science/r/KAN-GLNet-6432/ .
http://dx.doi.org/10.13039/501100001809 National Natural Science Foundation of China
32301762
Liu Jie
This project is supported by National Natural Science Foundation of China, grant number 32301762.
pmc-status-qastatus 0 pmc-status-live yes pmc-status-embargo no pmc-status-released yes pmc-prop-open-access yes pmc-pOpen asset ↗anonymous.4open.science/r/KAN-GLNet-6432lines:1-65Code / dataset availability confirmedEurope PMC · Crossref · checked 6 Sept 2026
Canola is an important economic and agronomic crop globally, but its yield is under threat due to climate change. Stomata are a key breeding target because of their importance in carbon capture and water use efficiency. However, screening for elite stomatal traits could be laborious and time-consuming. We developed a new toolkit called Stomatal Comprehensive Automated Neural Network or SCAN that combines the use of high-resolution portable digital microscopy with machine learning to automate stomatal trait phenotyping in canola. We show that SCAN can rapidly measure stomatal density, size, and pore area in canola at 97-99% accuracy, and capture real-time stomatal pore status that strongly correlated with leaf porometer measurement in canola. Here we use SCAN to investigate how leaf stomatal traits vary through a canopy in different ecotypes of canola grown in the field and glasshouse conditions. SCAN revealed that stomatal density in canola decreases in more expanded leaves with the abaxial surface having up to 40% more stomata that are 2× more open than the adaxial surface. SCAN also showed that patterns of stomatal traits in canola vary between leaf position in the canopy and change with environment in an ecotype-dependent manner.
Why it matches plant phenotyping methods葉の気孔形質を自動取得する画像・機械学習ツールを開発し、精度検証と既存測定との相関評価を行っており、植物表現型取得法が研究の中心である。
abstractWe developed a new toolkit called Stomatal Comprehensive Automated Neural Network or SCAN that combines the use of high-resolution portable digital microscopy with machine learning to automate stomatal trait phenotyping in canola.
Reproduction assets foundThe paper publicly releases its authors' analysis code, trained model weights, and training image datasets for the SCAN stomatal phenotyping pipeline via two GitHub repositories and two Roboflow datasets, with explicit availability statements in the Data availability section. Raw phenotype measurements are in a journalCode · publicThe full details of the weights, hyperparameters, training scripts, and datasets of the models can be found at https://github.com/William-Yao0993/FD_detection .Open asset ↗William-Yao0993/FD_detectionlines:45-53Dataset · publicThe microscopy images used to train the SCAN model are available as two public datasets: https://app.roboflow.com/danila-lab/fd-project-1 and https://app.roboflow.com/danila-lab/pore-segmentation/ .Open asset ↗danila-lab/fd-project-1lines:233-272Dataset · publicThe microscopy images used to train the SCAN model are available as two public datasets: https://app.roboflow.com/danila-lab/fd-project-1 and https://app.roboflow.com/danila-lab/pore-segmentation/ .Open asset ↗danila-lab/pore-segmentationlines:233-272Code / dataset availability confirmedCrossref · OpenAlex · Europe PMC · checked 6 Sept 2026
Understanding the genetic basis of quantitative traits related to crop growth, yield, and stress response requires the acquisition of large-scale, high-quality phenotypic datasets. High-throughput phenotyping platforms have become effective tools for meeting this requirement. Autonomous mobile robots have gained prominence owing to their ability to carry heavy payloads, their operational flexibility, and their proximity to crops, which allows for higher imaging resolution. In this study, we introduce PhenoRob-F (a phenotyping robot for the field), a cross-row, wheeled robot designed for efficient and automated phenotyping under field conditions. The mobile platform and phenotyping module of the robot were engineered to meet the specific demands of field phenotyping, with integrated visual and satellite navigation systems enabling autonomous operation. We validated the performance of the robot through a series of experiments involving various crop canopies. By capturing RGB images of rice and wheat, we independently performed wheat ear detection and rice panicle segmentation. For wheat ear detection, we achieve a precision of 0.783, a recall of 0.822, and a mean average precision (mAP) of 0.853 when the YOLOv8m model is used. For rice panicle segmentation, the SegFormer_B0 model yielded a mean intersection over union (mIoU) of 0.949 and an accuracy of 0.987. Additionally, by capturing RGB-D data of maize canopies, we performed 3D reconstructions to calculate plant height, achieving an R 2 of 0.99 compared with manual measurements. Similar experiments with rapeseed yielded an R 2 of 0.97. Near-infrared spectral data collected from drought-stressed rice plants enabled the classification of drought severity into five categories, with classification accuracies ranging from 0.977 to 0.996. Our results reveal that PhenoRob-F is an effective tool for high-throughput phenotyping and is capable of providing precise data to support phenotypic trait analysis and the selection of superior crop genotypes.
Why it matches plant phenotyping methods圃場用自律ロボットと複数の画像・分光センシング、形質抽出手法を開発し、作物キャノピーで性能検証しているため、植物フェノタイピング手法が研究の中心である。
abstractwe introduce PhenoRob-F (a phenotyping robot for the field), a cross-row, wheeled robot designed for efficient and automated phenotyping under field conditions.
Reproduction assets foundThe paper's data availability statement explicitly links a public GitHub repository containing part of the data and code supporting this PhenoRob-F phenotyping study; remaining data are available on request.Code · publicPart of the data and code supporting this study are openly available with the following link: https://github.com/balloonhaha/PhenoRob-F. All other reasonable requests for data and research materials will be fulfilled upon contacting the corresponding authors.Open asset ↗balloonhaha/PhenoRob-Fhtml-lines:193-220Code / dataset availability confirmedOpenAlex · arXiv · checked 15 Sept 2026
Quantitative descriptions of the complete canopy architecture are essential for accurately evaluating crop photosynthesis and yield performance to guide ideotype design. Although various sensing technologies have been developed for three-dimensional (3D) reconstruction of individual plants and canopies, they failed to obtain an accurate description of canopy architectures due to severe occlusion among complex canopy architectures. We proposed an effective method for 3D reconstruction of complex, dynamic population canopy architecture for rapeseed crops with a novel point cloud completion model. A complete point cloud generation framework was developed for automated annotation of the training dataset by distinguishing surface points from occluded points within canopies. The crop population point cloud completion network (CP-PCN) was then designed with a multi-resolution dynamic graph convolutional encoder (MRDG) and a point pyramid decoder (PPD) to predict occluded points. To further enhance feature extraction, a dynamic graph convolutional feature extractor (DGCFE) module was proposed to capture structural variations over the whole rapeseed growth period. The results demonstrated that CP-PCN achieved chamfer distance (CD) values of 3.35 cm -4.51 cm over four growth stages, outperforming the state-of-the-art transformer-based method (PoinTr). Ablation studies confirmed the effectiveness of the MRDG and DGCFE modules. Moreover, the validation experiment demonstrated that the silique efficiency index developed from CP-PCN improved the overall accuracy of rapeseed yield prediction by 11.2% compared to that of using incomplete point clouds. The CP-PCN pipeline has the potential to be extended to other crops, significantly advancing the quantitatively analysis of in-field population canopy architectures.
Why it matches plant phenotyping methods作物群落キャノピーの3D形態を復元する点群補完法を開発し、既存法との比較、アブレーション、収量予測への有効性検証まで行っており、植物フェノタイピング手法が研究の中心である。
abstractWe proposed an effective method for 3D reconstruction of complex, dynamic population canopy architecture for rapeseed crops with a novel point cloud completion model.
Reproduction assets foundThe paper's availability statement explicitly deposits all source code and test data (rapeseed canopy point cloud completion, CP-PCN) on GitHub at the allowed URL.Code · publicn Wang, Yi Feng,
Mengjie Gong and Guangyu Wu, for their participation in the experiments, and
to the Jiaxing Academy of Agricultural Sciences for their assistance with the
experimental data acquisition.
Availability of supporting data and source code
All source codes and test data involved in this study are available on
GitHub (https://github.com/Ziyue-Guo/RP-PCN.git).
Declaration of Competing Interest
The authors declare that they have no known competing financial interests
or personal relationships that could have appeared to influence the work
reported in this paper.
Contributions
Z. G. designed the study, conducted the experiments, and wrote the
manuscript. Y. S. contributed to the expeOpen asset ↗Ziyue-Guo/RP-PCNpdf-layout-page:42 lines:1-42Code / dataset availability confirmedOpenAlex · Crossref · Europe PMC · checked 6 Sept 2026
Introduction Recent advancements in sensor technologies have enabled collection of many large, high-resolution plant images datasets that could be used to non-destructively explore the relationships between genetics, environment and management factors on phenotype or the physical traits exhibited by plants. The phenotype data captured in these datasets could then be integrated into models of plant development and crop yield to more accurately predict how plants may grow as a result of changing management practices and climate conditions, better ensuring future food security. However, automated methods capable of reliably and efficiently extracting meaningful measurements of individual plant components (e.g. leaves, flowers, pods) from imagery of whole plants are currently lacking. In this study, we explore interdisciplinary application of MapReader, a computer vision pipeline for annotating and classifying patches of larger images that was originally developed for semantic exploration of historical maps, to time-series images of whole oilseed rape (Brassica napus) plants. Methods Models were trained to classify five plant structures in patches derived from whole plant images (branches, leaves, pods, flower buds and flowers), as well as background patches. Three modelling methods are compared: (i) 6-label multi-class classification, (ii) a chain of binary classifiers approach, and (iii) an approach combining binary classification of plant and background patches, followed by 5-label multi-class classification of plant structures. Results A combined plant/background binarization and 5-label multi-class modelling approach using a ‘resnext50d_4s2x40d’ model architecture for both the binary classification and multi-class classification components was found to produce the most accurate patch classification for whole B. napus plant images (macro-averaged F1-score = 88.50, weighted average F1-score = 97.71). This combined binary and 5-label multi-class classification approach demonstrate similar performance to the top-performing MapReader ‘railspace’ classification model. Discussion This highlights the potential applicability of the MapReader model framework to images data from across scientific and humanities domains, and the flexibility it provides in creating pipelines with different modelling approaches. The pipeline for dynamic plant phenotyping from whole plant images developed in this study could potentially be applied to imagery from varied laboratory conditions, and to images datasets of other plants of both agricultural and conservation concern.
Why it matches plant phenotyping methods植物全体画像から葉・花・莢などの構造を自動抽出・分類する動的フェノタイピング手法を開発・評価しており、方法が研究の中心である。
abstractautomated methods capable of reliably and efficiently extracting meaningful measurements of individual plant components (e.g. leaves, flowers, pods) from imagery of whole plants are currently lacking.
Reproduction assets foundThe paper's phenotyping analysis is based on a public RGB image dataset of Brassica napus plants, explicitly deposited by the authors with a public URL. MapReader is a generic pre-existing library and the HuggingFace railspace models are cited prior work, not paper-specific assets.Dataset · publicThe datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: https://research.aber.ac.uk/en/datasets/collection-of-side-view-and-top-view-rgb-images-of-brassica-napus .Open asset ↗research.aber.ac.uklines:982-1027Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Winter oilseed rape (WOSR, Brassica napus L.) is the third largest oil crop worldwide that also provides a source of high quality plant-based proteins. Nitrogen (N) and carbon (C) play a key role in plant growth. Determination of N and C contents of plant tissues throughout the growth cycle is crucial in assessing plant nutritional status and allowing precise input management. In the dataset presented in this article, 2427 WOSR samples arising from a large diversity of tissues collected on WOSR diversity were analyzed by near infrared spectroscopy from 4000 to 12,000 cm -1 . At the same time, reference chemical data for the N and C contents of the same samples were determined by elemental analysis using the Dumas method. Partial least squares regression has been used to develop predictive models linking spectral and chemical data, so that new samples can be characterized without the need for reference methods. This dataset could be used to test new calculation algorithms in order to enhance prediction performance or for training purposes. These models can be used as a rapid method for determining N and/or C content, adding to decision-support tools for fertilizer application throughout the plant developmental cycle.
Why it matches plant phenotyping methods植物組織の窒素・炭素含量を近赤外分光で推定する予測モデルと大規模データセットが研究の中心であり、植物形質・栄養状態の取得手法として実質的です。
abstractIn the dataset presented in this article, 2427 WOSR samples arising from a large diversity of tissues collected on WOSR diversity were analyzed by near infrared spectroscopy
Reproduction assets foundThe article is a Data in Brief describing a paper-specific public dataset of NIR spectra and N/C reference measurements for 2427 Brassica napus tissue samples, deposited in Data INRAE with an explicit DOI and direct URL. The dataset includes the raw spectral data (.csv), chemical reference data, and the PLS calibrationDataset · publicData source location
Institution:
Institute of Genetics, Environment and Plant Protection (IGEPP); INRAE,
Institut Agro, University of Rennes
City/Town/Region: 35,650 Le Rheu
Country: France
Data accessibility
Repository name: Data INRAE ( https://data.inrae.fr/ )
Data identification number: 10.57745/6VYUQN
Direct URL to data: https://entrepot.recherche.data.gouv.fr/dataset.xhtml?persistentId=doi:10.57745/6VYUQN
Related research article
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Value of the Data
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The dataset establishes a link between spectral properties and chemical composition (N, C) of a wide variety of plant tissues in winter oilseed rape. The prediction models can be used by diverse communities (scientists, breeders, prOpen asset ↗Data INRAE · 10.57745/6VYUQNlines:1-63Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
The present dataset combines transcriptomic and microscopic analyses to investigate the responses of winter oilseed rape (WOSR, Brassica napus L., cultivar Aviso) to soil drought, with a focus on differences between young and early-senescent old leaves. For microscopy, 36 scans of 1 to 5 leaf cross-sections were acquired from paraffin-embedded leaf disc samples using a scanner with a 40x lens (Pannoramic Confocal, 3DHistech), capturing a large field of view (8-mm-long observed leaf tissue). The raw scanned cross-sections and analyzed images are available under doi.org/10.57745/RK5PM3 in the Recherche Data Gouvrepository. These high-quality scans enable the differentiation of mesophyll cells and tissues. Software analysis yielded a dataset with 54 selected cross-sectional areas, 291 delimited surfaces of palisade, spongy, and vessel tissues, and 11,136 individually delimited cells from the palisade and spongy layers. For transcriptomics, an Illumina Novaseq sequencer was used to generate 390 Gb of mRNA paired-end reads. The raw reads were filtered, mapped, and assigned to genes from the Brassica napus reference genome Darmor-bzh v10, which were subsequently used to identify differentially expressed genes (DEGs) and to perform gene ontology enrichment analysis. The raw reads are accessible under accession PRJNA939927 at the NCBI Sequence Read Archive (SRA). This high-quality dataset provides insights into the molecular mechanisms underlying oilseed rape's response to soil drought and may aid in the development of drought-tolerant cultivars. A total of 17,975 DEGs were identified between well-watered and severe drought conditions across the contrasted leaf developmental stages.
Why it matches plant phenotyping methods葉の断面画像を取得・解析し、組織面積や個別細胞などの植物形態形質を構造化した再利用可能なデータセットを提供しており、画像ベースの表現型取得が実質的な構成要素である。
abstractFor microscopy, 36 scans of 1 to 5 leaf cross-sections were acquired from paraffin-embedded leaf disc samples using a scanner with a 40x lens
Reproduction assets foundThe article deposits its own plant-phenotyping assets publicly: raw and analyzed leaf cross-section microscopy scans (Recherche Data Gouv, doi:10.57745/RK5PM3) and the transcriptomic dataset (Recherche Data Gouv doi:10.57745/7HQSM3, mirrored at NCBI SRA under PRJNA939927). The analysis pipelines cited (nf-core/rnaseq, Dataset · publicThe raw scanned cross-sections and analyzed images are available under doi.org/10.57745/RK5PM3 in the Recherche Data Gouvrepository.Open asset ↗Recherche Data Gouv · 10.57745/RK5PM3lines:1-41Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Plant population counts are highly valued by crop producers as important early-season indicators of field health. Traditionally, emergence rate estimates have been acquired through manual counting, an approach that is labor-intensive and relies heavily on sampling techniques. By applying deep learning-based object detection models to aerial field imagery, accurate plant population counts can be obtained for much larger areas of a field. Unfortunately, current detection models often perform poorly when they are faced with image conditions that do not closely resemble the data found in their training sets. In this paper, we explore how specific facets of a plant detector's training set can affect its ability to generalize to unseen image sets. In particular, we examine how a plant detection model's generalizability is influenced by the size, diversity, and quality of its training data. Our experiments show that the gap between in-distribution and out-of-distribution performance cannot be closed by merely increasing the size of a model's training set. We also demonstrate the importance of training set diversity in producing generalizable models, and show how different types of annotation noise can elicit different model behaviors in out-of-distribution test sets. We conduct our investigations with a large and diverse dataset of canola field imagery that we assembled over several years. We also present a new web tool, Canola Counter, which is specifically designed for remote-sensed aerial plant detection tasks. We use the Canola Counter tool to prepare our annotated canola seedling dataset and conduct our experiments. Both our dataset and web tool are publicly available.
Why it matches plant phenotyping methods航空画像からカノーラ個体数（個体群密度）を推定する検出モデルの汎化性能を検証し、注釈付きデータセットと専用Webツールを提示しており、植物表現型取得手法が中心である。
abstractBy applying deep learning-based object detection models to aerial field imagery, accurate plant population counts can be obtained for much larger areas of a field.
Reproduction assets foundThe paper's aerial canola seedling dataset (images and annotations) is publicly deposited on Zenodo, and the authors' Canola Counter analysis/annotation tool is open source on GitHub. The arXiv 2108.05789 entry is a cited prior work (CropAndWeed dataset), not a paper-specific asset.Dataset · publicData Availability Statement
The canola seedling dataset used in this study is publicly available and can be found at: https://doi.org/10.5281/zenodo.11055599 . The Canola Counter tool is open source and is available at: https://github.com/eandvaag/agricounter .Open asset ↗zenodo · 10.5281/zenodo.11055599lines:236-237Code · publicData Availability Statement
The canola seedling dataset used in this study is publicly available and can be found at: https://doi.org/10.5281/zenodo.11055599 . The Canola Counter tool is open source and is available at: https://github.com/eandvaag/agricounter .Open asset ↗github · eandvaag/agricounterlines:236-237Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
To address the underestimation of rape yield by traditional gramineous crop yield simulation methods based on crop models, this study used the WOFOST crop model to estimate rape yield in the main producing areas of southern Hunan based on 2 years of field-measured data, with consideration given to the photosynthesis of siliques, which are non-foliar green organs. First, the total photosynthetic area index (TPAI), which considers the photosynthesis of siliques, was proposed as a substitute for the leaf area index (LAI) as the calibration variable in the model. Two parameter calibration methods were subsequently proposed, both of which consider photosynthesis by siliques: the TPAI-SPA method, which is based on the TPAI coupled with a specific pod area, and the TPAI-Curve method, which is based on the TPAI and curve fitting. Finally, the 2 proposed parameter calibration methods were validated via 2 years of observed rape data. The results indicate that compared with traditional LAI-based crop model calibration methods, the TPAI-SPA and TPAI-Curve methods can improve the accuracy of rape yield estimation. The estimation accuracy ( R 2 ) for the total weight of storage organs (TWSO) and above-ground biomass (TAGP) increased by 9.68% and 49.86%, respectively, for the TPAI-SPA method and by 14.04% and 42.94%, respectively, for the TPAI-Curve method. Thus, the 2 calibration methods proposed in this study are of important practical importance for improving the accuracy of rape yield simulations. This study provides a novel technical approach for utilizing crop growth models in the yield estimation of oilseed crops.
Why it matches plant phenotyping methods非葉部器官の光合成を組み込んだTPAIを新たな校正変数として提案し、作物モデルによる収量・バイオマス推定法を開発・検証しているため、表現型推定手法が中心である。
abstractthe total photosynthetic area index (TPAI), which considers the photosynthesis of siliques, was proposed as a substitute for the leaf area index (LAI) as the calibration variable in the model.
Reproduction assets foundThe paper's Data Availability statement explicitly deposits the authors' TPAI-SPA and TPAI-Curve calibration code/training scripts for the WOFOST rape yield estimation on GitHub at a public URL, which is an allowed URL and matches the paper's computational analysis.Code · publicThe code and training script of TPAI-SPA and TPAI-Curve has been hosted to GitHub and is available at https://github.com/rsw1998/TPAI-SPA-Curve-for-WOFOST .Open asset ↗rsw1998/TPAI-SPA-Curve-for-WOFOSTlines:675-747Code / dataset availability confirmedOpenAlex · Europe PMC · bioRxiv · checked 15 Sept 2026
Abstract Background Automating stomatal trait measurement has gained popularity because of their inherent importance for field phenotyping application as stomata are critical for both carbon capture and water use efficiency in plants. Such tool has been reported for rice, wheat, tomato, barley and oil palm. However, none exist yet for canola, which is an important economic and agronomic crop globally. Results We developed a new toolkit called Stomatal Comprehensive Automated Neural Network or SCAN by combining the use of high-resolution portable digital microscopy with machine learning based on You Only Look Once algorithm (YOLOv8). Digital micrographs of leaf surfaces enter the SCAN pipeline, which includes stomata detection, stomata segmentation and stomatal pore segmentation models, to measure stomatal density, stomatal size and stomatal pore area, respectively. In addition to SCAN’s ability to measure leaf stomatal traits in canola at 89 to 94% accuracy, we also showed that SCAN can be used to predict stomatal density even in species not included in the training set such as Arabidopsis, tobacco, rice, wheat, maize and proso millet. SCAN was designed for the biological science community with the premise that users are not required to possess advanced programming capabilities to manage dependency prerequisites, execute the models, and integrate the analysis. This was achieved by packaging the models into a desktop application system that can be accessed offline. Conclusion Overall, SCAN provides a non-destructive, real-time, portable, and high-throughput measurement of leaf stomatal traits in canola. The minimised hardware requirement and user-friendly desktop application system make SCAN suitable for field phenotyping application.
Why it matches plant phenotyping methodsカノーラ葉の気孔形質を画像と機械学習で自動抽出するツールを開発し、精度検証と他種での適用性評価を行っており、フェノタイピング手法が中心である。
abstractWe developed a new toolkit called Stomatal Comprehensive Automated Neural Network or SCAN by combining the use of high-resolution portable digital microscopy with machine learning based on You Only Look Once algorithm (YOLOv8).
Reproduction assets foundThe paper's authors publicly deposit the SCAN pipeline's model weights, hyperparameters, training scripts, and datasets in the FD_detection GitHub repository, and provide the SCAN application itself (with download and demonstration) in a second GitHub repository. Both are paper-specific, public, and actionable.Code · publicin Table S1.
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The training tasks were carried out on an Ubuntu 20.04 Linux server at the Research School of Biology in
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Australian National University, using two Nvidia A30 (24G) Graphic Processing Units (GPUs). The full
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details of models’ weights, hyperparameters, training scripts and datasets can be found at
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https://github.com/William-Yao0993/FD_detection.128
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Model evaluation
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Mean Average Precision (mAP, Fig. 3) and F1 score were used to assess model ability (Fig. 4). mAP is
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calculated as the mean value of each class area under the precision-recall curve over thresholds, and the
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F1 score is the harmonic mean of precision and recall. The formulas are deOpen asset ↗William-Yao0993/FD_detectionpdf-raw-page:4 lines:1-81Code / dataset availability confirmedOpenAlex · Crossref · Europe PMC · checked 7 Sept 2026
Background Autofluorescence-based imaging has the potential to non-destructively characterize the biochemical and physiological properties of plants regulated by genotypes using optical properties of the tissue. A comparative study of stress tolerant and stress susceptible genotypes of Brassica rapa with respect to newly introduced stress-based phenotypes using machine learning techniques will contribute to the significant advancement of autofluorescence-based plant phenotyping research. Methods Autofluorescence spectral images have been used to design a stress detection classifier with two classes, stressed and non-stressed, using machine learning algorithms. The benchmark dataset consisted of time-series image sequences from three Brassica rapa genotypes (CC, R500, and VT), extreme in their morphological and physiological traits captured at the high-throughput plant phenotyping facility at the University of Nebraska-Lincoln, USA. We developed a set of machine learning-based classification models to detect the percentage of stressed tissue derived from plant images and identified the best classifier. From the analysis of the autofluorescence images, two novel stress-based image phenotypes were computed to determine the temporal variation in stressed tissue under progressive drought across different genotypes, i.e., the average percentage stress and the moving average percentage stress. Results The study demonstrated that both the computed phenotypes consistently discriminated against stressed versus non-stressed tissue, with oilseed type (R500) being less prone to drought stress relative to the other two Brassica rapa genotypes (CC and VT). Conclusion Autofluorescence signals from the 365/400 nm excitation/emission combination were able to segregate genotypic variation during a progressive drought treatment under a controlled greenhouse environment, allowing for the exploration of other meaningful phenotypes using autofluorescence image sequences with significance in the context of plant science.
Why it matches plant phenotyping methods自家蛍光画像と機械学習により植物のストレス組織割合を抽出し、新規な時系列ストレス表現型を算出する方法が研究の中心である。
abstractWe developed a set of machine learning-based classification models to detect the percentage of stressed tissue derived from plant images and identified the best classifier.
Reproduction assets foundThe paper's autofluorescence image dataset (UNL-UW-AFD, 3360 images of three Brassica rapa genotypes) is explicitly stated to be publicly available for download at the authors' URL. No author analysis code with a public URL is stated.Dataset · publicwe built and made publicly available Autofluorescence Dataset collaboratively developed by the University of Nebraska–Lincoln and the University of Wyoming (UNL-UW-AFD) as a benchmark dataset, at https://plantvision.unl.edu/dataset . The dataset consists of 3360 autofluorescence images captured for three genotypes, i.e., R500 , CC , and VT .Open asset ↗plantvision.unl.edu · UNL-UW-AFDlines:339-346Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
Oilseed rape is an important oilseed crop planted worldwide. Maturity classification plays a crucial role in enhancing yield and expediting breeding research. Conventional methods of maturity classification are laborious and destructive in nature. In this study, a nondestructive classification model was established on the basis of hyperspectral imaging combined with machine learning algorithms. Initially, hyperspectral images were captured for 3 distinct ripeness stages of rapeseed, and raw spectral data were extracted from the hyperspectral images. The raw spectral data underwent preprocessing using 5 pretreatment methods, namely, Savitzky-Golay, first derivative, second derivative (D2nd), standard normal variate, and detrend, as well as various combinations of these methods. Subsequently, the feature wavelengths were extracted from the processed spectra using competitive adaptive reweighted sampling, successive projection algorithm (SPA), iterative spatial shrinkage of interval variables (IVISSA), and their combination algorithms, respectively. The classification models were constructed using the following algorithms: extreme learning machine, k -nearest neighbor, random forest, partial least-squares discriminant analysis, and support vector machine (SVM) algorithms, applied separately to the full wavelength and the feature wavelengths. A comparative analysis was conducted to evaluate the performance of diverse preprocessing methods, feature wavelength selection algorithms, and classification models, and the results showed that the model based on preprocessing-feature wavelength selection-machine learning could effectively predict the maturity of rapeseed. The D2nd-IVISSA-SPA-SVM model exhibited the highest modeling performance, attaining an accuracy rate of 97.86%. The findings suggest that rapeseed maturity can be rapidly and nondestructively ascertained through hyperspectral imaging.
Why it matches plant phenotyping methodsハイパースペクトル画像と機械学習により、ナタネの成熟状態を非破壊的に分類する取得・解析手法を開発し、前処理、波長選択、モデル性能を比較評価しているため、フェノタイピング手法が中心である。
abstracta nondestructive classification model was established on the basis of hyperspectral imaging combined with machine learning algorithms
Reproduction assets foundThe authors state that the primary script and dataset (spectral reflectance data and classification/feature-wavelength-extraction code) used in this rapeseed hyperspectral maturity classification study are publicly accessible via the provided link, which matches an allowed URL.Dataset · publicAll authors confirm that all raw experimental data are available upon request. The primary script and dataset used during the experimental procedure are accessible via the following link: http://plantphenomics.hzau.edu.cn/usercrop/Rice/download .Open asset ↗lines:421-450Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Background The flowering period is a critical time for the growth of rape plants. Counting rape flower clusters can help farmers to predict the yield information of the corresponding rape fields. However, counting in-field is a time-consuming and labor-intensive task. To address this, we explored a deep learning counting method based on unmanned aircraft vehicle (UAV). The proposed method developed the in-field counting of rape flower clusters as a density estimation problem. It is different from the object detection method of counting the bounding boxes. The crucial step of the density map estimation using deep learning is to train a deep neural network that maps from an input image to the corresponding annotated density map. Results We explored a rape flower cluster counting network series: RapeNet and RapeNet+. A rectangular box labeling-based rape flower clusters dataset (RFRB) and a centroid labeling-based rape flower clusters dataset (RFCP) were used for network model training. To verify the performance of RapeNet series, the paper compares the counting result with the real values of manual annotation. The average accuracy (Acc), relative root mean square error (rrMSE) and [Formula: see text] of the metrics are up to 0.9062, 12.03 and 0.9635 on the dataset RFRB, and 0.9538, 5.61 and 0.9826 on the dataset RFCP, respectively. The resolution has little influence for the proposed model. In addition, the visualization results have some interpretability. Conclusions Extensive experimental results demonstrate that the RapeNet series outperforms other state-of-the-art counting approaches. The proposed method provides an important technical support for the crop counting statistics of rape flower clusters in field.
Why it matches plant phenotyping methodsUAV-RGB画像から菜種の花房数という植物器官形質を推定する深層学習手法を開発・評価しており、フェノタイピング手法が中心である。
abstractwe explored a deep learning counting method based on unmanned aircraft vehicle (UAV).
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Code · publicThe codes, datasets RFRB and RFCP used in the study are available online at: https://github.com/CV-Wang/RapeNet .Open asset ↗CV-Wang/RapeNetlines:216-232Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Flowering is a crucial developing stage for rapeseed ( Brassica napus L.) plants. Flowers develop on the main and branch inflorescences of rapeseed plants and then grow into siliques. The seed yield of rapeseed heavily depends on the total flower numbers per area throughout the whole flowering period. The number of rapeseed inflorescences can reflect the richness of rapeseed flowers and provide useful information for yield prediction. To count rapeseed inflorescences automatically, we transferred the counting problem to a detection task. Then, we developed a low-cost approach for counting rapeseed inflorescences using YOLOv5 with the Convolutional Block Attention Module (CBAM) based on unmanned aerial vehicle (UAV) Red-Green-Blue (RGB) imagery. Moreover, we constructed a Rapeseed Inflorescence Benchmark (RIB) to verify the effectiveness of our model. The RIB dataset captured by DJI Phantom 4 Pro V2.0, including 165 plot images and 60,000 manual labels, is to be released. Experimental results showed that indicators R 2 for counting and the mean Average Precision (mAP) for location were over 0.96 and 92%, respectively. Compared with Faster R-CNN, YOLOv4, CenterNet, and TasselNetV2+, the proposed method achieved state-of-the-art counting performance on RIB and had advantages in location accuracy. The counting results revealed a quantitative dynamic change in the number of rapeseed inflorescences in the time dimension. Furthermore, a significant positive correlation between the actual crop yield and the automatically obtained rapeseed inflorescence total number on a field plot level was identified. Thus, a set of UAV- assisted methods for better determination of the flower richness was developed, which can greatly support the breeding of high-yield rapeseed varieties.
Why it matches plant phenotyping methodsUAV RGB画像と深層学習を用いてナタネの花序数を自動計数する手法を開発し、ベンチマークデータセットで検証しているため、植物表現型取得が中心である。
abstractwe developed a low-cost approach for counting rapeseed inflorescences using YOLOv5 with the Convolutional Block Attention Module (CBAM) based on unmanned aerial vehicle (UAV) Red-Green-Blue (RGB) imagery.
Reproduction assets foundThe paper's Rapeseed Inflorescence Benchmark (RIB) — 165 UAV RGB plot images with 60,000 manual inflorescence labels used for the counting model — is stated as publicly available at the authors' GitHub repository. The YOLOv5 repository is a generic third-party library, not a paper-specific asset.Dataset · publicg. Considering the insufficient data of the whole flowering period, we will increase the sampling frequency in flowering period to better fit the change curve of the number of rapeseed inflorescences in future work.
Data availability statement
Publicly available datasets were analyzed in this study. This data can be found here: https://github.com/LYLWYH/Rapeseed-Data .
Author contributions
All authors made significant contributions to this manuscript. JL, YL, and JQ performed field data collection and wrote the manuscript. JQ and LL designed the experiment. JY, XW, and GL provided suggestions on the experiment design. All authors read and approved the final manuscript.
Acknowledgments
A larOpen asset ↗LYLWYH/Rapeseed-Datalines:437-471Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Why it matches plant phenotyping methods植物の害虫抵抗性を取得する中スループット表現型プロトコルを開発し、代替法との比較検証と大規模適用まで行っており、表現型取得法が研究の中心である。
abstractHere we present a phenotyping protocol allowing mid-throughput (i.e., increased throughput compared to current methods) acquisition of resistance data, which could then be used for genetic mapping of QTLs.
Reproduction assets foundThe paper's pollen-beetle resistance phenotyping data (feeding damage on white mustard and OSR genotypes across whole-plant, miniaturized-plant, and detached-bud protocols) are openly deposited on Figshare per the authors' data availability statement.Dataset · publicetle www.soci.org
increases with the population size and the number of repetitions DATA AVAILABILITY STATEMENT
per individual,10–13 this protocol was tested on a large white mus- The data that support the findings of this study are openly available
tard population of 620 individual plants. We found significant var- in Figshare at https://figshare.com/account/articles/20764747.
iation in pollen beetle feeding damage among white mustard
genotypes, which indicates the potential for large-scale applica-
tion of this phenotyping protocol. Further improvements of this SUPPORTING INFORMATION
protocol can be envisaged. Placing plants and insects inside the Supporting information may be found in the onlOpen asset ↗Figshare · 20764747pdf-layout-page:6 lines:1-49Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Background Researchers interested in the seed size of rapeseed need to quantify the cell size and number of cells in the seed coat, embryo and silique wall. Scanning electron microscope-based methods have been demonstrated to be feasible but laborious and costly. After image preparation, the cell parameters are generally evaluated manually, which is time consuming and a major bottleneck for large-scale analysis. Recently, two machine learning-based algorithms, Trainable Weka Segmentation (TWS) and Cellpose, were released to overcome this long-standing problem. Moreover, the MorphoLibJ and LabelsToROIs plugins in Fiji provide user-friendly tools to deal with cell segmentation files. We attempted to verify the practicability and efficiency of these advanced tools for various types of cells in rapeseed. Results We simplified the current image preparation procedure by skipping the fixation step and demonstrated the feasibility of the simplified procedure. We developed three methods to automatically process multicellular images of various tissues in rapeseed. The TWS-Fiji (TF) method combines cell detection with TWS and cell measurement with Fiji, enabling the accurate quantification of seed coat cells. The Cellpose-Fiji (CF) method, based on cell segmentation with Cellpose and quantification with Fiji, achieves good performance but exhibits systematic error. By removing border labels with MorphoLibJ and detecting regions of interest (ROIs) with LabelsToROIs, the Cellpose-MorphoLibJ-LabelsToROIs (CML) method achieves human-level performance on bright-field images of seed coat cells. Intriguingly, the CML method needs very little manual calibration, a property that makes it suitable for massive-scale image processing. Through a large-scale quantitative evaluation of seed coat cells, we demonstrated the robustness and high efficiency of the CML method at both the single-cell level and the sample level. Furthermore, we extended the application of the CML method to developing seed coat, embryo and silique wall cells and acquired highly precise and reliable results, indicating the versatility of this method for use in multiple scenarios. Conclusions The CML method is highly accurate and free of the need for manual correction. Hence, it can be applied for the low-cost, high-throughput quantification of diverse cell types in rapeseed with high efficiency. We envision that this method will facilitate the functional genomics and microphenomics studies of rapeseed and other crops.
Why it matches plant phenotyping methodsアブラナの種皮・胚・長角果壁の細胞形質を画像から自動定量する手法を開発・検証しており、フェノタイピング手法が研究の中心です。
abstractWe developed three methods to automatically process multicellular images of various tissues in rapeseed.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Dataset · publicA cell image of 30 DAF silique wall acquired under 100 × optical microscope.Open asset ↗lines:547-634Code / dataset availability confirmedCrossref · checked 8 Sept 2026
Biophysical parameter retrieval using remote sensing has long been utilized for crop yield forecasting and economic practices. Remote sensing can provide information across a large spatial extent and in a timely manner within a season. Plant Area Index (PAI), Vegetation Water Content (VWC), and Wet-Biomass (WB) play a vital role in estimating crop growth and helping farmers make market decisions. Many parametric and non-parametric machine learning techniques have been utilized to estimate these parameters. A general non-parametric approach that follows a Bayesian framework is the Gaussian Process (GP). The parameters of this process-based technique are assumed to be random variables with a joint Gaussian distribution. The purpose of this work is to investigate Gaussian Process Regression (GPR) models to retrieve biophysical parameters of three annual crops utilizing combinations of multiple polarizations from C-band SAR data. RADARSAT-2 full-polarimetric images and in situ measurements of wheat, canola, and soybeans obtained from the SMAPVEX16 campaign over Manitoba, Canada, are used to evaluate the performance of these GPR models. The results from this research demonstrate that both the full-pol (HH+HV+VV) combination and the dual-pol (HV+VV) configuration can be used to estimate PAI, VWC, and WB for these three crops.
Why it matches plant phenotyping methodsSARデータとGPRモデルにより作物のPAI・VWC・湿重量バイオマスを推定する手法を開発・評価しており、植物形質取得が研究の中心である。
abstractThe purpose of this work is to investigate Gaussian Process Regression (GPR) models to retrieve biophysical parameters of three annual crops utilizing combinations of multiple polarizations from C-band SAR data.
Reproduction assets foundThe paper's Data Availability Statement explicitly provides a public GitHub repository containing the authors' GPR analysis code for crop biophysical parameter retrieval from RADARSAT-2 data. The in situ SMAPVEX16-MB measurements and RADARSAT-2 imagery themselves are not stated as publicly released by the authors.Code · publicData Availability Statement: The code for the present work is available at: https://github.com/
Swarnendu-sekhar-ghosh/GPR_biophysical_parameter_retrieval_RS2, accessed 15 February 2022.Open asset ↗Swarnendu-sekhar-ghosh/GPR_biophysical_parameter_retrieval_RS2pdf-page:24 lines:1-60Code / dataset availability confirmedOpenAlex · Crossref · checked 15 Sept 2026
With the ongoing cost decrease of genotyping and sequencing technologies, accurate and fast phenotyping remains the bottleneck in the utilizing of plant genetic resources for breeding and breeding research. Although cost-efficient high-throughput phenotyping platforms are emerging for specific traits and/or species, manual phenotyping is still widely used and is a time- and money-consuming step. Approaches that improve data recording, processing or handling are pivotal steps towards the efficient use of genetic resources and are demanded by the research community. Therefore, we developed PhenoApp, an open-source Android app for tablets and smartphones to facilitate the digital recording of phenotypical data in the field and in greenhouses. It is a versatile tool that offers the possibility to fully customize the descriptors/scales for any possible scenario, also in accordance with international information standards such as MIAPPE (Minimum Information About a Plant Phenotyping Experiment) and FAIR (Findable, Accessible, Interoperable, and Reusable) data principles. Furthermore, PhenoApp enables the use of pre-integrated ready-to-use BBCH (Biologische Bundesanstalt für Land- und Forstwirtschaft, Bundessortenamt und CHemische Industrie) scales for apple, cereals, grapevine, maize, potato, rapeseed and rice. Additional BBCH scales can easily be added. The simple and adaptable structure of input and output files enables an easy data handling by either spreadsheet software or even the integration in the workflow of laboratory information management systems (LIMS). PhenoApp is therefore a decisive contribution to increase efficiency of digital data acquisition in genebank management but also contributes to breeding and breeding research by accelerating the labour intensive and time-consuming acquisition of phenotyping data.
Why it matches plant phenotyping methods植物表現型データのデジタル記録・取得を目的とするオープンソースアプリの開発であり、表現型測定ワークフローが中心的です。
abstractTherefore, we developed PhenoApp, an open-source Android app for tablets and smartphones to facilitate the digital recording of phenotypical data in the field and in greenhouses.
Reproduction assets foundThe paper describes PhenoApp, an open-source Android phenotyping app. Authors provide the app's source code (Gitea, archived on Zenodo) and underlying example input/output phenotype data files on Zenodo under CC0. The SHAPE II project website is a project page, not a paper-specific data deposit, and is excluded.Code · publice ‘in’ folder of the app main directory and no additional source data is required).
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Output_example.xls (sample output file created by PhenoApp).
Data are available under the terms of the
Creative Commons Zero “No rights reserved” data waiver (CC0 1.0 Public domain dedication).
Software availability
Source code available from:
https://gitea.julius-kuehn.de/JKI/pheno-app
Archived source code at time of publication:
https://doi.org/10.5281/zenodo.5525779
36
License:
Apache-2.0
Acknowledgements
We are grateful to Moritz Cappel, Teresa Claus and Claudia Vogel for ongoing testing, recommendations and bug report of PhenoApp during development.
Funding Statement
This work was supported by grants fOpen asset ↗gitea.julius-kuehn.de · JKI/pheno-applines:333-433Code · publicput_example.xls (sample output file created by PhenoApp).
Data are available under the terms of the
Creative Commons Zero “No rights reserved” data waiver (CC0 1.0 Public domain dedication).
Software availability
Source code available from:
https://gitea.julius-kuehn.de/JKI/pheno-app
Archived source code at time of publication:
https://doi.org/10.5281/zenodo.5525779
36
License:
Apache-2.0
Acknowledgements
We are grateful to Moritz Cappel, Teresa Claus and Claudia Vogel for ongoing testing, recommendations and bug report of PhenoApp during development.
Funding Statement
This work was supported by grants from the German Federal Ministry of Education and Research to FS (SelWineQ, FKZ 031B0889Open asset ↗Zenodo · 10.5281/zenodo.5525779lines:333-433Code / dataset availability confirmedOpenAlex · Crossref · Europe PMC · checked 8 Sept 2026
Phenotyping is considered a significant bottleneck impeding fast and efficient crop improvement. Similar to many crops, Brassica napus, an internationally important oilseed crop, suffers from low genetic diversity, and will require exploitation of diverse genetic resources to develop locally adapted, high yielding and stress resistant cultivars. A pilot study was completed to assess the feasibility of using indoor high-throughput phenotyping (HTP), semi-automated image processing, and machine learning to capture the phenotypic diversity of agronomically important traits in a diverse B. napus breeding population, SKBnNAM, introduced here for the first time. The experiment comprised 50 spring-type B. napus lines, grown and phenotyped in six replicates under two treatment conditions (control and drought) over 38 days in a LemnaTec Scanalyzer 3D facility. Growth traits including plant height, width, projected leaf area, and estimated biovolume were extracted and derived through processing of RGB and NIR images. Anthesis was automatically and accurately scored (97% accuracy) and the number of flowers per plant and day was approximated alongside relevant canopy traits (width, angle). Further, supervised machine learning was used to predict the total number of raceme branches from flower attributes with 91% accuracy (linear regression and Huber regression algorithms) and to identify mild drought stress, a complex trait which typically has to be empirically scored (0.85 area under the receiver operating characteristic curve, random forest classifier algorithm). The study demonstrates the potential of HTP, image processing and computer vision for effective characterization of agronomic trait diversity in B. napus, although limitations of the platform did create significant variation that limited the utility of the data. However, the results underscore the value of machine learning for phenotyping studies, particularly for complex traits such as drought stress resistance.
Why it matches plant phenotyping methods屋内ハイスループット表現型解析、画像処理、機械学習を用いて作物形質を抽出・予測し、プラットフォーム性能も評価しているため、方法が研究の中心である。
abstractA pilot study was completed to assess the feasibility of using indoor high-throughput phenotyping (HTP), semi-automated image processing, and machine learning to capture the phenotypic diversity of agronomically important traits
Reproduction assets foundThe paper's full LemnaTec HTP image dataset (RGB, NIR, FLUOR, HYP images of 50 B. napus founder lines) is openly available at the authors' P2IRC USask repository, directly reproducing this paper's phenotyping measurements. The genomevis tool concerns SNP/genotype visualization, not phenotyping, and no analysis code is,Dataset · publicThe full image dataset is openly available at https://p2irc-data-dev.usask.ca/dataset/10.1109.SciDataManager.2020.7284788 (Dataset name: P2IRC Flagship 1 Data).Open asset ↗P2IRC Flagship 1 Data · 10.1109.SciDataManager.2020.7284788lines:323-329Code / dataset availability confirmedCrossref · checked 14 Sept 2026
Abstract Fractional vegetation cover (FVC) is the key trait of interest for characterizing crop growth status in crop breeding and precision management. Accurate quantification of FVC among different breeding lines, cultivars, and growth environments is challenging, especially because of the large spatiotemporal variability in complex field conditions. This study presents an ensemble modeling strategy for phenotyping crop FVC from unmanned aerial vehicle (UAV)-based multispectral images by coupling the PROSAIL model with a gap probability model (PROSAIL-GP). Seven field experiments for four main crops were conducted, and canopy images were acquired using a UAV platform equipped with RGB and multispectral cameras. The PROSAIL-GP model successfully retrieved FVC in oilseed rape (Brassica napus L.) with coefficient of determination, root mean square error (RMSE), and relative RMSE (rRMSE) of 0.79, 0.09, and 18%, respectively. The robustness of the proposed method was further examined in rice (Oryza sativa L.), wheat (Triticum aestivum L.), and cotton (Gossypium hirsutum L.), and a high accuracy of FVC retrieval was obtained, with rRMSEs of 12%, 6%, and 6%, respectively. Our findings suggest that the proposed method can efficiently retrieve crop FVC from UAV images at a high spatiotemporal domain, which should be a promising tool for precision crop breeding.
Why it matches plant phenotyping methodsUAVマルチスペクトル画像から作物のFVCという形態・生育形質を推定するモデルを開発し、複数作物・圃場実験で精度と頑健性を検証しており、表現型取得手法が研究の中心である。
abstractThis study presents an ensemble modeling strategy for phenotyping crop FVC from unmanned aerial vehicle (UAV)-based multispectral images by coupling the PROSAIL model with a gap probability model (PROSAIL-GP).
Reproduction assets foundThe paper's Data availability statement explicitly deposits the authors' PROSAIL-GP model code and all datasets (UAV-derived canopy reflectance/FVC measurements) in a public GitHub repository, plus detailed protocols on protocols.io. The PROSAIL model itself is a generic prior tool and is excluded.Code · publicle.
Conflict of interest
The authors declare no conflict of interest.
Data availability
Data supporting this work,such as details and source code of the PROSAIL
model used in this study,are openly available at http://teledetection.ipgp.jussieu.fr/prosail/.The code of the PROSAIL-GP model and all of the
datasets are available at https://github.com/WanLiangZJU/Crop-FVC-retrieval. The detailed protocols can be found at protocols.io (https://
dx.doi.org/10.17504/protocols.io.btmynk7w).
References
Aballa A, Cen H, Wan L, Mehmood K, He Y. 2020. Nutrient status diag-
nosis of infield oilseed rape via deep learning-enabled dynamic model. IEEE
Transactions on Industrial Informatics 17, 4379–4389.
BacOpen asset ↗WanLiangZJU/Crop-FVC-retrievalpdf-raw-page:15 lines:1-89Code / dataset availability confirmedEurope PMC · Crossref · checked 9 Sept 2026
Rapeseed is an important oil crop in China. Timely estimation of rapeseed stand count at early growth stages provides useful information for precision fertilization, irrigation, and yield prediction. Based on the nature of rapeseed, the number of tillering leaves is strongly related to its growth stages. However, no field study has been reported on estimating rapeseed stand count by the number of leaves recognized with convolutional neural networks (CNNs) in unmanned aerial vehicle (UAV) imagery. The objectives of this study were to provide a case for rapeseed stand counting with reference to the existing knowledge of the number of leaves per plant and to determine the optimal timing for counting after rapeseed emergence at leaf development stages with one to seven leaves. A CNN model was developed to recognize leaves in UAV-based imagery, and rapeseed stand count was estimated with the number of recognized leaves. The performance of leaf detection was compared using sample sizes of 16, 24, 32, 40, and 48 pixels. Leaf overcounting occurred when a leaf was much bigger than others as this bigger leaf was recognized as several smaller leaves. Results showed CNN-based leaf count achieved the best performance at the four- to six-leaf stage with F-scores greater than 90% after calibration with overcounting rate. On average, 806 out of 812 plants were correctly estimated on 53 days after planting (DAP) at the four- to six-leaf stage, which was considered as the optimal observation timing. For the 32-pixel patch size, root mean square error (RMSE) was 9 plants with relative RMSE (rRMSE) of 2.22% on 53 DAP, while the mean RMSE was 12 with mean rRMSE of 2.89% for all patch sizes. A sample size of 32 pixels was suggested to be optimal accounting for balancing performance and efficiency. The results of this study confirmed that it was feasible to estimate rapeseed stand count in field automatically, rapidly, and accurately. This study provided a special perspective in phenotyping and cultivation management for estimating seedling count for crops that have recognizable leaves at their early growth stage, such as soybean and potato.
Why it matches plant phenotyping methodsUAV画像とCNNを用いて rapeseed の葉を認識し、植物体数（stand count）を自動推定する手法の開発・性能評価が研究の中心であるため、植物フェノタイピング方法論に該当します。
abstractA CNN model was developed to recognize leaves in UAV-based imagery, and rapeseed stand count was estimated with the number of recognized leaves.
Reproduction assets foundThe paper's data availability statement explicitly deposits the 'Rapeseed_seedling_counting' data (supporting the UAV imagery-based stand count findings) in a public GitHub repository with an authors' URL, qualifying as a paper-specific public asset.Dataset · publicThe “Rapeseed_seedling_counting” data that support the findings of this study are available in “LARSC-Lab/Rapeseed_seedling_counting” in GitHub, which can be found at https://github.com/LARSC-Lab/Rapeseed_seedling_counting .Open asset ↗LARSC-Lab/Rapeseed_seedling_countinglines:590-664Code / dataset availability confirmedOpenAlex · Europe PMC · checked 14 Sept 2026
Rapeseed is the second most important oil crop species and is widely cultivated worldwide. However, overcoming the 'phenotyping bottleneck' has remained a significant challenge. A clear goal of high-throughput phenotyping is to bridge the gap between genomics and phenomics. In addition, it is important to explore the dynamic genetic architecture underlying rapeseed plant growth and its contribution to final yield. In this work, a high-throughput phenotyping facility was used to dynamically screen a rapeseed intervarietal substitution line population during two growing seasons. We developed an automatic image analysis pipeline to quantify 43 dynamic traits across multiple developmental stages, with 12 time points. The time-resolved i-traits could be extracted to reflect shoot growth and predict the final yield of rapeseed. Broad phenotypic variation and high heritability were observed for these i-traits across all developmental stages. A total of 337 and 599 QTLs were identified, with 33.5% and 36.1% consistent QTLs for each trait across all 12 time points in the two growing seasons, respectively. Moreover, the QTLs responsible for yield indicators colocalized with those of final yield, potentially providing a new mechanism of yield regulation. Our results indicate that high-throughput phenotyping can provide novel insights into the dynamic genetic architecture of rapeseed growth and final yield, which would be useful for future genetic improvements in rapeseed.
Why it matches plant phenotyping methodsアブラナの高スループット表現型解析施設を用い、自動画像解析パイプラインを開発して43の動的形質を定量化しており、表現型取得・抽出手法が研究の中心である。
abstracta high-throughput phenotyping facility was used to dynamically screen a rapeseed intervarietal substitution line population during two growing seasons.
Reproduction assets foundThe paper explicitly deposits its rapeseed phenotyping data (RGB images, genotypic and phenotypic i-trait data for both growing seasons) in the HZAU plant phenomics database, and its image analysis pipeline source code (LabVIEW, DLL, cpp, test images) on the first author's public GitHub repositories. Both are paper-‐‑Dataset · publice points (every ~7 days starting from 53 to 138 days after sowing). The trials were performed using a randomized block design with five replications in each growing season: 2015–2016 and 2016–2017. The screening generated a total of 1.62 terabytes of RGB images (16,986,93 images; PNG format), which are available in a database ( http://plantphenomics.hzau.edu.cn/search_rape.action , 2015‐2016‐QTL and 2016‐2017‐QTL). A movie of the growth of the recurrent parent and two select ISLs is shown in Movies [Link] , [Link] , [Link] . The inspected lines and inspection dates are shown in Table S1 , where T1‐T12 represent the twelve time points. In our greenhouse experiment, the final yield per plant wOpen asset ↗plantphenomics.hzau.edu.cnlines:165-171Code · publiceasons in this study are available at http://plantphenomics.hzau.edu.cn/search_rape.action under the sections 2015‐2016‐QTL and 2016‐2017‐QTL. The phenotypic data are also shown in Table S13 . All the source code, including that of LabVIEW programs, the dynamic link library, cpp documents and test images, can be downloaded from https://github.com/fenghuifh2006?tab=repositories .
Conflicts of interest
The authors declare that they have no conflicts of interest.
Author Contributions
H.L., H.F. and W.Y. performed the experiments, analysed the data and wrote the manuscript. C.G., S.Y., W.H., X.X., J.L. G.C. and Q.L. assisted in the data analysis and database information construction. W.Y., L.X. Open asset ↗github.com/fenghuifh2006lines:189-223Code / dataset availability confirmedOpenAlex · checked 15 Sept 2026
Crop leaf purpling is a common phenotypic change when plants are subject to some biotic and abiotic stresses during their growth. The extraction of purple leaves can monitor crop stresses as an apparent trait and meanwhile contributes to crop phenotype analysis, monitoring, and yield estimation. Due to the complexity of the field environment as well as differences in size, shape, texture, and color gradation among the leaves, purple leaf segmentation is difficult. In this study, we used a U-Net model for segmenting purple rapeseed leaves during the seedling stage based on unmanned aerial vehicle (UAV) RGB imagery at the pixel level. With the limited spatial resolution of rapeseed images acquired by UAV and small object size, the input patch size was carefully selected. Experiments showed that the U-Net model with the patch size of 256 × 256 pixels obtained better and more stable results with a F-measure of 90.29% and an Intersection of Union (IoU) of 82.41%. To further explore the influence of image spatial resolution, we evaluated the performance of the U-Net model with different image resolutions and patch sizes. The U-Net model performed better compared with four other commonly used image segmentation approaches comprising support vector machine, random forest, HSeg, and SegNet. Moreover, regression analysis was performed between the purple rapeseed leaf ratios and the measured N content. The negative exponential model had a coefficient of determination (R²) of 0.858, thereby explaining much of the rapeseed leaf purpling in this study. This purple leaf phenotype could be an auxiliary means for monitoring crop growth status so that crops could be managed in a timely and effective manner when nitrogen stress occurs. Results demonstrate that the U-Net model is a robust method for purple rapeseed leaf segmentation and that the accurate segmentation of purple leaves provides a new method for crop nitrogen stress monitoring.
Why it matches plant phenotyping methodsUAV画像から紫色葉という植物ストレス表現型を抽出するセグメンテーション手法の開発・比較が中心であり、植物フェノタイピング方法論に該当する。
abstractThe extraction of purple leaves can monitor crop stresses as an apparent trait and meanwhile contributes to crop phenotype analysis, monitoring, and yield estimation.
Reproduction assets foundThe paper's Data Availability statement links a public figshare deposit containing the rapeseed UAV image/segmentation datasets used in this study. No author code or trained model deposit is stated.Dataset · publicadded,
and purple leaf area will be assessed as a visual trait to find the optimal nitrogen threshold for
balancing crop yield and environmental impact. Moreover, other crops and stress types (e.g., water
stress) will be studied based on purple leaves.
Data Availability: The rapeseed datasets of this experience are available at
https://figshare.com/s/e7471d81a1e35d5ab0d1
Author Contributions: All authors have read and agreed to the published version of the manuscript. J.Z. and
T.X. designed the method, conducted the experiment, analyzed the data, discussed the results, and wrote the
majority of the manuscript. C.Y. guided the study design, advised on data analysis, and revised the manuscriptOpen asset ↗figsharepdf-raw-page:13 lines:1-34Code / dataset availability confirmedOpenAlex · Europe PMC · checked 10 Sept 2026
The use of spectral imaging within the plant phenotyping and breeding community has been increasing due its utility as a non-invasive diagnostic tool. However, there is a lack of imaging systems targeted specifically at plant science duties, resulting in low precision for canopy-scale measurements. This study trials a prototype multispectral system designed specifically for plant studies and looks at its use as an early detection system for visually asymptomatic disease phases, in this case Pyrenopeziza brassicae in Brassica napus . The analysis takes advantage of machine learning in the form of feature selection and novelty detection to facilitate the classification. An initial study into recording the morphology of the samples is also included to allow for further improvement to the system performance. The proposed method was able to detect light leaf spot infection with 92% accuracy when imaging entire oilseed rape plants from above, 12 days after inoculation and 13 days before the appearance of visible symptoms. False colour mapping of spectral vegetation indices was used to quantify disease severity and its distribution within the plant canopy. In addition, the structure of the plant was recorded using photometric stereo, with the output influencing regions used for diagnosis. The shape of the plants was also recorded using photometric stereo, which allowed for reconstruction of the leaf angle and surface texture, although further work is needed to improve the fidelity due to uneven lighting distributions, to allow for reflectance compensation. The ability of active multispectral imaging has been demonstrated along with the improvement in time taken to detect light leaf spot at a high accuracy. The importance of capturing structural information is outlined, with its effect on reflectance and thus classification illustrated. The system could be used in plant breeding to enhance the selection of resistant cultivars, with its early and quantitative capability.
Why it matches plant phenotyping methods植物向けマルチスペクトル撮像システムと画像解析法を開発・実証し、病徴前の病害検出、重症度・分布、植物構造を定量化しているため、フェノタイピング手法が中心である。
abstractThis study trials a prototype multispectral system designed specifically for plant studies and looks at its use as an early detection system for visually asymptomatic disease phases
Reproduction assets foundThe paper's Availability of data and materials statement deposits the raw MSI and photometric stereo datasets from both trials (canopy and detached leaf assays) on Mendeley Data, a public repository with a DOI, directly reproducing this paper's phenotyping measurements.Dataset · publicThe MSI and PS datasets for both trials undertaken are available in RAW format, compatible with all ENVI enabled software packages, from Mendeley Data ( https://doi.org/10.17632/ydmtggnzbw.1 ).Open asset ↗Mendeley Data · 10.17632/ydmtggnzbw.1lines:358-484Code / dataset availability confirmedOpenAlex · Europe PMC · checked 15 Sept 2026
Boron (B) is an essential micronutrient for seed plants. Information on B-efficiency mechanisms and B-efficient crop and model plant genotypes is very scarce. Studies evaluating the basis and consequences of B-deficiency and B-efficiency are limited by the facts that B occurs as a trace contaminant essentially everywhere, its bioavailability is difficult to control and soil-based B-deficiency growth systems allowing a high-throughput screening of plant populations have hitherto been lacking. The crop plant Brassica napus shows a very high sensitivity towards B-deficient conditions. To reduce B-deficiency-caused yield losses in a sustainable manner, the identification of B-efficient B. napus genotypes is indispensable. We developed a soil substrate-based cultivation system which is suitable to study plant growth in automated high-throughput phenotyping facilities under defined and repeatable soil B conditions. In a comprehensive screening, using this system with soil B concentrations below 0.1 mg B (kg soil)-1, we identified three highly B-deficiency tolerant B. napus cultivars (CR2267, CR2280 and CR2285) amongst a genetically diverse collection comprising 590 accessions from all over the world. The B-efficiency classification of cultivars was based on a detailed assessment of various physical and high-throughput imaging-based shoot and root growth parameters in soil substrate or in in vitro conditions, respectively. We identified cultivar-specific patterns of B-deficiency-responsive growth dynamics. Elemental analysis revealed striking differences only in B contents between contrasting genotypes when grown under B-deficient but not under standard conditions. Results indicate that B-deficiency tolerant cultivars can grow with a very limited amount of B which is clearly below previously described critical B-tissue concentration values. These results suggest a higher B utilization efficiency of CR2267, CR2280 and CR2285 which would represent a unique trait amongst so far identified B-efficient B. napus cultivars which are characterized by a higher B-uptake capacity. Testing various other nutrient deficiency treatments, we demonstrated that the tolerance is specific for B-deficient conditions and is not conferred by a general growth vigor at the seedling stage. The identified B-deficiency tolerant cultivars will serve as genetic and physiological ‘tools’ to further understand the mechanisms regulating the B nutritional status in rapeseed and to develop B-efficient elite genotypes.
Why it matches plant phenotyping methods土壌B条件を制御した自動ハイスループット表現型解析システムを開発し、画像ベースの生長形質で590系統を評価しており、表現型取得基盤が研究の中心的役割を担う。
abstractWe developed a soil substrate-based cultivation system which is suitable to study plant growth in automated high-throughput phenotyping facilities under defined and repeatable soil B conditions.
Reproduction assets foundThe paper's phenotyping measurements (590-accession B-efficiency screen, root cessation assay, imaging-derived traits, substrate nutrient quantification) are distributed as Supplementary Data Sheets S1–S5, publicly available via the Frontiers article's supplementary material page. No author analysis code or trained模型的专Supplement · publiccation number: 031A053).
1
www.fao.org
2
https://gbis.ipk-gatersleben.de/gbis2i/
3
https://gbis.ipk-gatersleben.de/gbis2i/
4
http://www.ipk-gatersleben.de/en/dept-genebank/satellite-collections-north/
5
http://apps.fas.usda.gov/psdonline/
Supplementary Material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2018.01142/full#supplementary-material
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Click here for additioOpen asset ↗lines:224-299Code / dataset availability confirmedCrossref · checked 10 Sept 2026
Abstract. Ground reference data are a prerequisite for the calibration, update, and validation of retrieval models facilitating the monitoring of land parameters based on Earth Observation data. Here, we describe the acquisition of a comprehensive ground reference database which was created to test and validate the recently developed Earth Observation Land Data Assimilation System (EO-LDAS) and products derived from remote sensing observations in the visible and infrared range. In situ data were collected for seven crop types (winter barley, winter wheat, spring wheat, durum, winter rape, potato, and sugar beet) cultivated on the agricultural Gebesee test site, central Germany, in 2013 and 2014. The database contains information on hyperspectral surface reflectance factors, the evolution of biophysical and biochemical plant parameters, phenology, surface conditions, atmospheric states, and a set of ground control points. Ground reference data were gathered at an approximately weekly resolution and on different spatial scales to investigate variations within and between acreages. In situ data collected less than 1 day apart from satellite acquisitions (RapidEye, SPOT 5, Landsat-7 and -8) with a cloud coverage ≤ 25 % are available for 10 and 15 days in 2013 and 2014, respectively. The measurements show that the investigated growing seasons were characterized by distinct meteorological conditions causing interannual variations in the parameter evolution. Here, the experimental design of the field campaigns, and methods employed in the determination of all parameters, are described in detail. Insights into the database are provided and potential fields of application are discussed. The data will contribute to a further development of crop monitoring methods based on remote sensing techniques. The database is freely available at PANGAEA (https://doi.org/10.1594/PANGAEA.874251).
Why it matches plant phenotyping methods複数作物の植物パラメータ、表現型、ハイパースペクトル反射を体系的に取得した地上基準データベースであり、取得設計と各パラメータの測定法を詳細に記述して、リモートセンシングモデルの校正・検証に用いる点が中心的です。
abstractwe describe the acquisition of a comprehensive ground reference database which was created to test and validate the recently developed Earth Observation Land Data Assimilation System (EO-LDAS) and products derived from remote sensing observations
Reproduction assets foundThis is a data descriptor paper whose plant-phenotyping measurements (biophysical/biochemical plant parameters, phenology, hyperspectral reflectance, FVC/PSM, soil moisture, photos, survey data) are explicitly deposited as public PANGAEA datasets with DOIs listed in the text. Multiple paper-specific public assets are直接Dataset · publicThe database is freely available at PANGAEA
(https://doi.org/10.1594/PANGAEA.874251).Open asset ↗PANGAEA · 10.1594/PANGAEA.874251pdf-page:1 lines:1-54