Pepper / chilliPotatoTomatoLeafClassificationVisualization / data managementDisease symptoms / severity
Early detection of plant leaf diseases is critical for minimizing crop losses and supporting precision agriculture. While Convolutional Neural Networks (CNNs) have demonstrated high accuracy in image-based diagnosis, conventional architectures may not optimally balance spatial localization and channel-wise feature refinement, particularly in multi-crop classification settings. This study proposes a redundancy-aware dual-attention architecture, termed ATSA-DenseNet, which integrates the spatial branch of the Convolutional Block Attention Module (CBAM-Spatial) with Efficient Channel Attention (ECA) within a DenseNet121 backbone. Unlike prior dual-attention frameworks that retain full CBAM and introduce channel-level redundancy, the proposed design isolates complementary spatial and channel mechanisms to improve representational efficiency without increasing computational complexity. The framework is evaluated on controlled multi-crop PlantVillage-derived datasets comprising tomato, potato, pepper, and maize. Across both 3-crop and 4-crop configuration, ATSA-DenseNet consistently outperforms baseline DenseNet121 and single-attention variants, achieving 99.94% accuracy and 0.9994 macro-F1 on the 4-crop setting while maintaining a lightweight footprint (6.96M parameters, 2.87G FLOPs). Grad-CAM visualizations indicate improved localization of disease-relevant regions compared to the baseline. While results are obtained under controlled imaging conditions, the findings demonstrate that redundancy-aware dual-attention enhances feature discrimination efficiency in multi-class agricultural classification tasks. Future work will extend validation to real-field datasets with natural variability.
Why it matches plant phenotyping methods植物葉の病害状態を画像から推定するCNN手法を提案・評価しており、病害表現型の取得・分類手法が研究の中心である。
abstractThis study proposes a redundancy-aware dual-attention architecture, termed ATSA-DenseNet, which integrates the spatial branch of the Convolutional Block Attention Module (CBAM-Spatial) with Efficient Channel Attention (ECA) within a DenseNet121 backbone.
Agricultural operations such as pepper harvesting, fruit counting, and field phenotyping rely on accurate visual recognition and instance segmentation algorithms. However, pepper fruits in complex field environments often exhibit slender and curved shapes, partial occlusion, ambiguous boundaries, and adhesion between adjacent instances. Existing object detection and instance segmentation methods therefore struggle to obtain complete fruit masks, which adversely affects subsequent fruit counting, contour measurement, and picking-point localization. To improve the instance segmentation accuracy of occluded peppers in complex field scenes, this study proposes OccPepSeg-YOLO, an improved model based on YOLO11n-seg. First, a P2FreqFusion module is introduced to fuse shallow, high-resolution detail features with deep semantic features, thereby enhancing the representation of fruit edges and tip regions. Second, an ASC module is designed to model the directional and scale-related morphological characteristics of pepper fruits, while a BoundaryGate module strengthens responses at occlusion interfaces and boundaries between adjacent instances. Finally, an OccPepSegment multi-scale prototype segmentation head is constructed, and a BDoU loss function is introduced to improve the boundary consistency of instance masks. Experiments on a self-constructed field-pepper instance segmentation dataset showed that OccPepSeg-YOLO achieved M-P, M-R, M-mAP50, and M-mAP50–95 values of 93.87%, 92.09%, 97.17%, and 82.31%, respectively, representing improvements of 5.59, 3.18, 3.83, and 9.52 percentage points over YOLO11n-seg. Further comparisons with representative YOLO-based instance segmentation models, including YOLOv8n-seg, YOLOv9c-seg, YOLO12n-seg, and YOLOv26n-seg, demonstrated that OccPepSeg-YOLO achieved the best overall segmentation performance. In particular, its M-mAP50–95 exceeded the best competing result obtained by YOLOv9c-seg by 8.35 percentage points. Under a unified repeated-inference protocol on an RTX 3090 GPU using FP32 precision, a batch size of 1, and 640 × 640 inputs, OccPepSeg-YOLO achieved a mean inference latency of 15.801 ± 1.238 ms, a P95 latency of 17.323 ms, and a throughput of 63.29 FPS. These results demonstrate that the proposed model can produce more complete pepper instance masks under leaf occlusion, fruit overlap, and complex background conditions, providing technical support for field-pepper recognition, fruit counting, and visual perception by agricultural robots.
Why it matches plant phenotyping methods圃場画像からピーマン果実のインスタンスマスクを抽出する手法を開発・比較検証しており、果実カウントや輪郭計測に利用可能な植物形質取得が中心である。
abstractAgricultural operations such as pepper harvesting, fruit counting, and field phenotyping rely on accurate visual recognition and instance segmentation algorithms.
Abstract Purpose : The system for diagnosing diseases in Solanaceae crops (SolanAPP), including tomatoes, potatoes, peppers, and eggplants, represents a promising tool for supporting decision-making in agricultural fields using AI. This system reconciles the computational intensity of multitasking models with the infrastructural limitations of rural environments, thereby increasing digital literacy. Its architecture is based on two fundamental pillars: (i) autonomous, offline operation for the detection and classification of diseases in Solanaceae crops; (ii) a georeferenced epidemiological surveillance network with agricultural recommendations for crop monitoring. Methods : The core diagnostic process combines crop-specific semantic segmentation and disease classification models exported to TensorFlow Lite, enabling on-device visual inference and pixel-level severity estimation. An optional online layer integrates Groq’s large language model (LLM)-based reasoning and Firebase services to generate structured agronomic explanations and facilitate the creation of georeferenced community reports when a connection is available. Preprocessing and management of the dataset were performed using the Roboflow platform. The mobile app was developed natively in Kotlin. Model performance was rigorously evaluated using accuracy, recall, F1 score, mean IoU, mPA, inference latency, model size, and decision matrix. The optimal model for the crop was selected using Simple Additive Weighting (SAW). Finally, the overall framework quality and usability were evaluated in Cuba through a user validation survey using a 5-point Likert scale and aligned with the ISO/IEC 25010 software quality model. Results : The model that yielded the best results for most crops was DeepLabV3+ with MobileNetV2, which achieved a classification accuracy of over 97\% while operating with lower inference latency. Beyond individual diagnoses, the system incorporates a collaborative georeferencing feature that allows users to share observations and precise geographic coordinates of detected pathologies to facilitate regional epidemiological monitoring. The user satisfaction survey yielded a satisfaction rating of 4.5/5, with users highlighting the importance of offline diagnosis. Conclusion : Plant disease diagnosis using computer vision can support earlier intervention in resource-constrained agricultural settings, but practical deployment requires models that are accurate, lightweight, interpretable, and usable under limited connectivity. SolanAPP, an offline-first Android framework for detecting foliar pathologies in Solanaceae crops, not only establishes a framework for disease identification in complex natural environments but also provides a theoretical and practical foundation for automated agronomic treatment recommendations and community-based crop surveillance. Impact SolanAPP is a free framework that supports the synergy between multitask deep learning for offline disease diagnosis and LLM-driven reasoning for decision-making in the field. Beyond the quantitative metrics obtained from the selected models, the deployment of SolanAPP in rural contexts serves a fundamental socio-technical purpose: it acts as a catalyst for open access, digital literacy, and agronomic decision-making under unfavorable development conditions. It also represents a strong effort to foster a collaborative epidemiological surveillance network in the agricultural sector. Although it faces challenges, such as the use of field images for model training, this framework marks a promising step in the deployment of edge AI, balancing technical accuracy with practical utility.
Why it matches plant phenotyping methods植物病害を画像からセグメンテーションし、病害のピクセル単位の重症度を推定する手法と、オフライン実装・性能評価を中心とした研究であり、植物状態の計測方法が中核です。
abstractThe core diagnostic process combines crop-specific semantic segmentation and disease classification models exported to TensorFlow Lite, enabling on-device visual inference and pixel-level severity estimation.
Abstract Plant diseases represent major constraints on agricultural productivity, often resulting in significant yield losses. This study presents FitoView, a cloud-based mobile decision support system that integrates deep learning with real-time weather forecasting for sustainable plant disease management. Demonstrated through a case study on Cercospora leaf spot in chili pepper, the system employs custom YOLOv8 models for automated disease detection, classification, and pixel-level severity quantification, combined with meteorological data from the OpenMeteo API. The core innovation lies in an integrated decision matrix that considers three dimensions: AI-assessed disease severity, 48-hour climatic risk forecasts, and optimal spraying conditions, generating four contextualized management scenarios with tailored re-evaluation periods (3-10 days). OpenMeteo API validation across four cities in Sergipe demonstrated very strong correlations, with Pearson coefficients (r) of 0.90-0.97 for temperature, 0.81-0.95 for humidity, and 0.92-0.95 for solar radiation, corresponding to R² values of 0.65-0.94. The YOLOv8 object detection model achieved perfect precision (100%) and macro-averaged recall of 89% across all disease classes, with Cercospora leaf spot detection reaching perfect metrics (100% precision, recall, and F1-score). Field validation in Lagarto, Sergipe, confirmed the system’s practical use: it accurately detected Cercospora leaf spot, estimated severity, and, combined with climatic risk, generated recommendations for alternative treatment and short-term re-evaluation. The Progressive Web Application architecture, deployed on a Cloud Platform, ensures accessibility without installation requirements, while the modular design enables scalability to additional crops and diseases, representing a significant advancement toward democratizing AI-powered precision agriculture tools for smallholder farmers in Brazil.
Why it matches plant phenotyping methods植物病害の検出・分類と病斑のピクセルレベル重症度推定をYOLOv8で実装・検証したシステムであり、植物状態の取得・定量化が中心的な技術貢献です。
abstractThe core innovation lies in an integrated decision matrix that considers three dimensions: AI-assessed disease severity, 48-hour climatic risk forecasts, and optimal spraying conditions
The prompt identification and precise categorization of chili plant diseases are crucial for promoting sustainable agriculture and reducing crop losses due to pests and pathogens. This research presents an automated detection method using the Single Shot Detector (SSD) framework, with ResNet-50 as the backbone and a Feature Pyramid Network (FPN) to manage multi-scale feature representations. Dataset including 14,248 images of chili plants, categorized into six classes like Leaf Spot, Rotten Fruit, Healthy Fruit, Healthy Leaf, Mosaic Curl, and Yellowing, underwent preprocessing involving segmentation, resizing, and augmentation, followed by a division into 90% training data and 10% testing data. Transfer learning was implemented using a COCO-pretrained SSD ResNet-50 FPN model, enhanced with cosine-decay learning-rate scheduling and momentum optimization. The assessment results indicated an overall accuracy of 92.7%, with the highest F1-scores achieved for Healthy Fruit (0.965) and Rotten Fruit (0.967). Under the COCO evaluation protocol, the model achieved an mAP@0.5 of 91.5% and mAP@[0.5:0.95] of 65.2%. Model ran at approximately 30 FPS on an NVIDIA T4 GPU. Reduced precision values were noted for Leaf Spot (0.866) and Mosaic Curl (0.850), suggesting a propensity for misclassification due to visual similarities among disease symptoms. Nonetheless, all classes attained F1-scores exceeding 0.86, illustrating the robustness of the proposed model. Importantly, the SSD-ResNet-50 approach offers both efficiency and accuracy within a single pipeline, enabling rapid inference practical for real-world applications. These findings emphasize the potential of deep learning-based solutions to strengthen plant disease monitoring systems. In conclusion, SSD with ResNet-50 FPN provides an effective and scalable methodology for the automated identification of chili plant diseases, contributing directly to sustainable agriculture and improved crop management practices.
Why it matches plant phenotyping methods植物の病徴・病害状態を画像から推定する深層学習手法の開発と評価が中心であり、植物フェノタイピング手法として適格。
abstractThis research presents an automated detection method using the Single Shot Detector (SSD) framework, with ResNet-50 as the backbone and a Feature Pyramid Network (FPN) to manage multi-scale feature representations.
Plant disease detection is critical for sustainable agriculture and food security. While deep learning models achieve high accuracy in leaf disease classification, their black box nature poses limitations for trust and adoption among agricultural practitioners. This study presents a comparative evaluation of three convolutional neural network architectures (ConvNeXt-Tiny, MobileNetV2, and VGG16) for classifying potato, maize, and pepper leaf diseases, with emphasis on explainability through Gradient-weighted Class Activation Mapping (Grad-CAM). The experimental results demonstrate that ConvNeXt-Tiny achieves 99-100% accuracy across all plant species, MobileNetV2 attains 97-100% accuracy with lower computational requirements, and VGG16 yields 97-99.5% accuracy. Grad-CAM visualizations reveal that modern architectures precisely focus on lesion regions, whereas older models occasionally attend to irrelevant features such as leaf veins and edges. Misclassification analysis identifies shadows and natural leaf patterns as primary error sources. This research demonstrates that explainable artificial intelligence is not merely complementary but essential for developing trustworthy agricultural decision support systems.
Why it matches plant phenotyping methods植物葉の病変領域を画像から分類・可視化する手法を比較評価しており、病害状態の表現型抽出が研究の中心です。
abstractThis study presents a comparative evaluation of three convolutional neural network architectures (ConvNeXt-Tiny, MobileNetV2, and VGG16) for classifying potato, maize, and pepper leaf diseases, with emphasis on explainability through Gradient-weighted Class Activation Mapping (Grad-CAM).
Abstract Chili is an important economic and nutritional crop with a relatively limited availability of different disease-resistant varieties. Leaf diseases, including those caused by fungi, bacteria, viruses, pests, or nutritional deficiencies, significantly compromise production and crop quality. Early detection of these diseases is key to reducing yield loss; however, traditional visual examinations are limited by time constraints, human subjectivity, and low detection sensitivity at early stages of infection. To overcome these challenges, this study proposes a deep learning–based framework for early multi-class detection of chili leaf diseases using convolutional neural networks (CNNs). A real-field dataset comprising 16,392 high-resolution images of chili leaves across six disease and healthy classes was collected from multiple regions of Bangladesh. The dataset was preprocessed, augmented, and split into training and validation sets using an 80:20 ratio. Five pre-trained CNN architectures—DenseNet121, EfficientNetB3, MobileNetV2, ResNet50, and InceptionV3 were evaluated using a transfer learning strategy. Experimental results demonstrate that MobileNetV2 achieved the best performance, attaining an overall classification accuracy of 96%. The results indicate that the proposed system demonstrates strong generalization capability and effectively discriminates visually similar chili leaf diseases. This work can be considered a valuable application in precision agriculture, providing an efficient, automated, and practical approach for in situ early diagnosis of chili leaf diseases through smart farm management.
Why it matches plant phenotyping methods唐辛子葉の病害状態を画像から分類するCNN手法の開発・比較評価とデータセット構築が研究の中心であり、植物病害フェノタイピングに該当する。
abstractA real-field dataset comprising 16,392 high-resolution images of chili leaves across six disease and healthy classes was collected from multiple regions of Bangladesh.
Chili plants (Capsicum sp.) are one of the important horticultural commodities in Indonesia with high economic value. However, chili productivity is often reduced due to leaf diseases such as leaf curl, yellow leaf virus, and leaf spot disease. Manual disease identification conducted by farmers still has several limitations because it requires considerable time, experience, and is prone to observation errors. Therefore, an automatic system is needed to support early detection of chili leaf diseases quickly and accurately. This study aims to develop a chili leaf disease classification system using a Convolutional Neural Network (CNN) based on leaf images. The dataset used in this study consists of chili leaf images categorized into four classes, namely healthy leaves, leaf curl, yellow leaf, and leaf spot. The research stages include dataset collection, image preprocessing, data augmentation, CNN model training, and model evaluation using a confusion matrix with performance metrics including accuracy, precision, recall, and F1-score. The results show that the CNN model is capable of classifying chili leaf diseases with satisfactory performance. Based on the evaluation results, the model achieved a precision value of 0.75, a recall value of 0.53, and a mean Average Precision (mAP@0.5) value of 0.60. The developed system is also able to display classification results along with the confidence score of the prediction. Therefore, the CNN method has strong potential to be implemented as an image-based early detection system for chili leaf diseases to assist farmers in monitoring plant conditions more effectively.
Why it matches plant phenotyping methods葉画像から健全・各種病徴を分類するCNNシステムの開発と性能評価が研究の中心であり、植物の病害状態を直接推定する画像ベース表現型計測に該当する。
abstractThis study aims to develop a chili leaf disease classification system using a Convolutional Neural Network (CNN) based on leaf images.
Smartphone-based precision spraying aims to restrict chemical application to target organs, but it also requires an organ-level perception model that operates on RGB alone at deployment, without dedicated depth sensors. Combining with monocular depth foundation models such as Depth Anything V2, RGB images can be enriched with dense pseudo-depth during training, turning close-range crop segmentation into a privileged-depth-guided learning problem where depth is available at training but absent at inference. Based on such close-range handheld capture, intra-foreground depth separability collapses while only the foreground–background depth gap remains partially preserved, exposing an imaging regime that we refer to as Close-Range Depth Degeneracy (CRDD) and that undermines the discriminative value of monocular depth for organ segmentation. Even with the available privileged-depth signal, existing RGB-D fusion and depth-branch hallucination routes still either introduce inference-time depth dependence or fail to retain the depth-conditioned effect inside RGB-side parameters under CRDD. How monocular pseudo-depth can serve as training-only privileged information for RGB-only close-range crop spraying under CRDD remains insufficiently studied. To this end, this paper proposes a privileged-depth modulation framework, MDM-Seg, by converting monocular depth from an inference-time modality into a CRDD-conditioned training signal to remove the inference-time depth dependence. Specifically, we characterize CRDD and propose a four-indicator depth-usability diagnostic that delivers a pre-training verdict on the target dataset, with verdicts validated against post-training segmentation outcomes. Then, we formalize the CRDD-conditioned training intervention as a depth-conditioned affine network (DepAN) that injects depth-gradient cues into decoder features, and define a depth hard-pixel mining loss (DHPM) to reweight per-pixel cross-entropy with depth-value evidence, both operating inside the main segmentation stream. Finally, we deploy MDM-Seg on RGB input alone and derive a lightweight consistency trace between the RGB-only and depth-conditioned heads, producing an image-level reliability cue for targeted-versus-fallback spraying without depth at inference. Extensive experiments on Pepper-Field-3940 demonstrate that our MDM-Seg achieves effective depth-conditioned RGB-only segmentation under CRDD, i.e., depth-conditioned modulation retained inside RGB-side parameters with no inference-time depth dependence, such as mIoU 0.8995 with the ResNet-101 configuration ( + 3.73 pp over the RGB-only DeepLabV3+ baseline), a lightweight training-side DepAN module, and an image-level reliability cue rank-correlated with segmentation quality (Spearman ρ = 0.74 ). On a Jetson Orin NX 16 GB with TensorRT FP16, the complete deployment-oriented MobileNetV2 dual-head prescription pipeline runs at 23.87 ms per image, corresponding to 41.89 FPS, 0.62 GB peak memory, and 0.56 J per image.
Why it matches plant phenotyping methods植物器官の画像セグメンテーション手法を開発・評価し、散布対象器官の状態をRGB画像から推定することが中心であるため。
abstractthis paper proposes a privileged-depth modulation framework, MDM-Seg
Abstract Crop disease is a major worldwide problem in agricultural production and food security, adversely affecting yield and quality for a variety of plant species. To overcome these drawbacks, this research provides a Double Transfer Learning-based Capsule Network (DTL-CapsNet) approach for automated plant disease classification with multiple crops. Based on the image pre-processing, segmentation, double transfer learning, and Capsule Networks technologies, the proposed framework extracts discriminative features of the diseases and maintains spatial relations between the leaves symptoms effectively. This double transfer learning approach involves extracting general visual features from pre-trained deep learning models and then fine-tuning these features to classify plant diseases. Capsule Networks then leverage the visual similarity of disease patterns to make the recognition more robust, while simultaneously adding hierarchical part–whole relationships in leaf structures, thereby improving the feature representation. Experiments were performed on a heterogeneous data set consisting of 21,927 leaf images belonging to 17 different healthy and diseased classes of apple, chilli, cotton, corn and potato crops. The experimental results proposed DTL-CapsNet framework is more accurate compared to the traditional CNN-based models and conventional transfer learning models. The proposed method of double transfer learning and Capsule Networks offers an efficient and scalable approach for intelligent plant disease diagnosis, offering significant potential in precision agriculture and real-time crop monitoring systems.
Why it matches plant phenotyping methods葉画像から植物病害状態を分類する画像・計算手法が研究の中心であり、提案手法の開発と既存モデルとの比較検証が行われているため。
abstractBased on the image pre-processing, segmentation, double transfer learning, and Capsule Networks technologies, the proposed framework extracts discriminative features of the diseases and maintains spatial relations between the leaves symptoms effectively.
Abstract Plant diseases affecting leaves and fruits cause substantial yield and economic losses worldwide, particularly in horticultural crops cultivated under diverse agro-climatic conditions. Early and accurate disease diagnosis is essential for effective crop management; however, manual inspection is time-consuming, subjective, and often infeasible at large scale. In this work, we present the Tomato–Chilli–Papaya (TCP) Fruit and Leaf Disease Dataset, a comprehensive multi-crop image dataset designed to support deep learning-based plant disease recognition. The dataset comprises labeled RGB images of healthy and diseased leaves and fruits from three economically important crops—tomato, chilli, and papaya—captured under real-field and semi-controlled environments, reflecting significant variability in illumination, background complexity, and disease severity. To demonstrate the applicability of the dataset, several commonly used convolutional neural network (CNN) architectures, including VGG, ResNet, DenseNet, MobileNet, and EfficientNet models, were trained and evaluated on the TCP dataset using transfer learning. Experimental results show that deep CNN models can effectively learn discriminative visual features corresponding to disease-specific patterns such as leaf spots, lesions, discoloration, curling, and fruit surface abnormalities. Lightweight models such as MobileNet achieve competitive performance with reduced computational cost, while deeper architectures provide improved accuracy at the expense of higher complexity. The results highlight the importance of dataset diversity for robust model generalization across multiple crops and plant organs. The TCP dataset provides a challenging benchmark for single-crop and multi-crop disease classification and supports the development of advanced deep learning, attention-based, and explainable AI models for precision agriculture. By enabling reproducible research and realistic performance evaluation, this dataset contributes toward scalable and practical AI-driven plant disease diagnosis systems aimed at reducing yield losses and supporting sustainable agriculture.
Why it matches plant phenotyping methods植物の葉・果実の病徴を画像から評価する大規模データセットとベンチマークを中心に扱っており、植物病害状態の画像ベース表現型解析に該当する。
abstractwe present the Tomato–Chilli–Papaya (TCP) Fruit and Leaf Disease Dataset, a comprehensive multi-crop image dataset designed to support deep learning-based plant disease recognition.
Reproduction assets foundThe paper introduces the TCP (Tomato-Chilli-Papaya) fruit and leaf disease image dataset and reports CNN experiments on it. The dataset is publicly deposited on Mendeley Data, and the authors state that analysis code is available on GitHub. Both are paper-specific, public, and actionable.Dataset · publicData is available on Mendeley:1Open asset ↗pdf-page:27 lines:1-51Code · publicCode availability: Code is available on GitHub 2Open asset ↗GitHubpdf-page:27 lines:1-51Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Traditional manual grading of fresh chili peppers suffers from inconsistent quality control and low efficiency. To meet the demand for accurate fruit shape recognition during the post-harvest stage, this study proposes an intelligent recognition method based on an improved DenseNet-121 network. This approach facilitates the application of machine vision in agricultural sorting equipment. DenseNet-121 serves as the backbone network. The Convolutional Block Attention Module (CBAM) is introduced to enhance feature focus on fruit shapes. A regularization strategy (Dropout = 0.3, weight decay = 1 × 10 -4 ) and a cross-entropy loss function with label smoothing (LS = 0.1) are integrated to optimize decision boundaries. These configurations prevent the model from overfitting to hard training labels and yield a robust classification architecture. Experimental results demonstrate that the proposed model achieves a precision of 90.09%, a recall of 89.60%, an F1-score (the harmonic mean of precision and recall) of 89.53%, and an overall accuracy of 89.74%. The model contains 7.09 M parameters and requires a single-frame inference time of 7.35 ms. Comprehensive evaluations indicate that the proposed model achieves an optimal balance among environmental noise robustness, prediction accuracy, and computational efficiency. Consequently, by maintaining high fine-grained classification accuracy alongside a low memory footprint and rapid inference speed, the model demonstrates strong potential for real-time deployment on resource-constrained edge devices within actual agricultural optical sorting equipment.
Why it matches plant phenotyping methodsチリペッパー果実の形状という植物器官形質を画像から分類する深層学習手法の開発・評価が中心であり、単なる品質測定ではない。
abstractExperimental results demonstrate that the proposed model achieves a precision of 90.09%, a recall of 89.60%, an F1-score (the harmonic mean of precision and recall) of 89.53%, and an overall accuracy of 89.74%.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Dataset · publicThe original image dataset is provided as Supplementary Materials .Open asset ↗lines:30-40Plant phenotyping relevance match · UnverifiedEurope PMC · checked 5 Sept 2026
Background Breeding for cold tolerance in pepper (Capsicum annuum L.) is critical to mitigate yield losses caused by unpredictable temperature fluctuations associated with climate change. However, genetic improvement of this trait is hindered by challenges in accurate phenotyping, particularly at the adult stage, and by its complex genetic architecture involving numerous minor-effect loci. While genomic selection (GS) offers a promising solution to accelerate genetic gain, its predictive ability is often limited by statistical noise from uninformative markers within whole-genome marker sets. This study aimed to overcome this limitation by developing a robust phenotypic index and implementing a genome-wide association study (GWAS)-informed GS strategy. Results We phenotyped 192 pepper accessions from a core collection for cold tolerance using a visual survival score (Surv) and a newly developed composite cold-tolerance index (CTI). Both CTI (h 2 = 0.55) and Surv (h 2 = 0.53) showed moderate heritability, suggesting a substantial contribution from additive genetic variance to the phenotypic variation of cold tolerance in adult plants. GWAS identified 13 candidate genomic regions associated with cold tolerance; these regions included TRM9, CAP1, and PP2A-2, genes previously implicated in abiotic stress responses. For genomic prediction, we applied nested CV and LOOCV in which GWAS and marker selection were performed within the training set before fitting the prediction model, so that phenotypic information from the test individuals was not incorporated into the marker selection step. Compared with the full marker set of 73,502 markers, the best GWAS-selected marker sets achieved prediction accuracies of 0.237 for CTI and 0.197 for Surv in nested CV, and 0.349 for CTI and 0.294 for Surv in LOOCV. At the same marker numbers and model conditions, random marker sets showed lower accuracies of 0.205 and 0.166 for the nested CV, and 0.068 and - 0.064 for the LOOCV, respectively. Conclusions Our study demonstrates that assessing cold tolerance via the CTI helps overcome the limitations of discrete survival scoring. By turning ordinal data into a continuous spectrum, the CTI can unmask hidden genetic variation. In addition, GWAS identified candidate genomic regions and genes associated with cold response, and nested CV and LOOCV showed that GWAS-selected marker sets could achieve higher prediction accuracy than the full marker set and random marker sets of the same marker number. This integrated framework offers a practical approach for interpreting the genetic basis of adult-stage cold tolerance in pepper and improving the efficiency of genomic prediction models for complex abiotic stress traits.
Why it matches plant phenotyping methods成体ペッパーの耐寒性を測定する新規複合表現型指標(CTI)の開発・評価が明示され、単なる形質の routine 測定を超えて表現型取得法の中心的貢献となっている。
abstractThis study aimed to overcome this limitation by developing a robust phenotypic index and implementing a genome-wide association study (GWAS)-informed GS strategy.
Accurate detection of plant leaf diseases is essential for enhancing crop productivity and supporting global food security. In addition to disease classification, understanding how environmental and soil conditions affect model performance is important for developing robust real-world agricultural monitoring systems. Although deep learning (DL) models achieve high accuracy on benchmark datasets, their performance in real-world settings is often limited by variations in illumination, background complexity, and environmental conditions. This study proposes a smart DL framework for detecting and classifying multiple leaf diseases in tomato, potato, and pepper plants. The framework combines U2-Net-based leaf segmentation with a Convolutional Neural Network–Bidirectional Gated Recurrent Unit (CNN–Bi-GRU) architecture. MobileNetV2 is employed as the feature extraction backbone to capture spatial characteristics, while Bi-GRU layers model sequential feature dependencies, forming a spatio-temporal network whose architectural design prioritizes parameter efficiency through depthwise separable convolutions and reduced gating complexity. The model was trained and validated using the PlantVillage benchmark dataset and achieved a classification accuracy of 99.8% with a macro-averaged F1-score of 94%, outperforming several state-of-the-art architectures. To assess robustness under real-world conditions, the trained model was further tested on leaf images collected from open-field environments near Eluru, South India. The field evaluation revealed a reduction in classification accuracy to 61.97%, indicating the impact of domain shift and environmental variability. To investigate potential contributing factors, soil parameters, including pH, temperature, moisture, and NPK levels, were monitored using an IoT-based Arduino sensing system over ten consecutive days. Rather than serving as direct inputs to the disease classification model, these environmental measurements were analyzed to assess their potential influence on disease symptom expression and the observed reduction in model performance under field conditions. The results suggest that environmental conditions may influence disease symptom expression and model transferability. This study highlights the importance of integrating DL-based disease recognition with environmental monitoring for reliable field-level agricultural applications. Nevertheless, computational complexity metrics, including inference latency and memory footprint, were not evaluated in the present work and are identified as a priority for future edge deployment studies.
Why it matches plant phenotyping methods植物葉の病徴を画像から分類するセグメンテーション・深層学習手法を開発し、ベンチマークと圃場画像で性能を検証しているため、植物フェノタイピング手法が中心である。
abstractThis study proposes a smart DL framework for detecting and classifying multiple leaf diseases in tomato, potato, and pepper plants.
Precise identification of chili flowers and buds is essential for enabling intelligent robotic pollination, continuous crop monitoring, and early yield prediction in protected horticulture. Reliable visual sensing remains challenging because chili targets are extremely small, densely distributed, and frequently occluded by foliage, while greenhouse environments introduce strong illumination variations and background reflections. These factors often lead to insufficient feature extraction and unstable detection accuracy in existing models, limiting their practical deployment in automated monitoring systems. To address these challenges, this study proposes GCP-YOLO, a lightweight yet high-performance detection framework built upon the YOLOv11n architecture. The model enhances small-target perception through three key improvements. First, a redesigned Generalized Feature Pyramid Network (GFPN) strengthens cross-scale feature interaction, improving the fusion of fine-grained texture cues and deep semantic information. Second, a C2CGA context-guided attention module is introduced to emphasize floral structural features while suppressing background noise caused by reflections and canopy clutter. Third, extended multi-scale detection heads (P2-P6) incorporate broader contextual information to reduce missed detections and false positives in dense planting scenarios. Experimental results on a custom chili flower dataset show that the proposed method achieves 92.8% precision, 83.7% recall, 90.8% mAP50, and 72.7% mAP50-95, improving upon the YOLOv11n baseline by 2.1, 1.3, 3.9, and 6.6 percentage points, respectively. Deployment on an NVIDIA Jetson AGX Orin edge platform demonstrates real-time inference at 97.9 FPS, confirming its suitability for on-device phenotyping. Overall, the proposed approach significantly improves detection robustness under complex greenhouse conditions, providing an effective visual sensing methodology for automated crop monitoring and data-driven yield estimation.
Why it matches plant phenotyping methodsチリの花・蕾という植物器官の画像検出手法を中心に、モデル改良、専用データセットでの性能評価、エッジ実装まで行っており、植物表現型取得の技術的貢献が明確である。
titleGCP-YOLO: high-precision detection of tiny chili flowers in complex greenhouse scenes.
Leaf water status is a key indicator for irrigation scheduling and early stress detection, but many spectroscopic prediction studies have mainly relied on near-infrared features. Here, practical prediction models were developed using mid-infrared (MIR) ATR-FTIR spectra of capsicum (Capsicum annuum L.) leaves collected under glasshouse conditions during a 10-day gradual dehydration period, alongside an irrigated control. Spectra (4000-450 cm⁻1) were measured with minimal sample preparation, and leaf water traits were quantified using fuel moisture content (FMC), equivalent water thickness (EWT), and specific leaf weight (SLW). Water-related MIR bands at 3370 and 1641 cm⁻1 showed the most consistent response to dehydration, and simple band ratios generally provided stronger predictions than single bands. The best ratios were A1641/A2159 for FMC (R2 = 0.81; RMSE = 12.80) and A3370/A2849 for EWT (R2 = 0.72; RMSE = 0.0034) and SLW (R2 = 0.62; RMSE = 6.95 × 10⁻4), while predicted-versus-measured performance yielded R2 values of 0.72 for FMC, 0.68 for EWT, and 0.52 for SLW. These results indicate that MIR ATR-FTIR spectroscopy, when coupled with selected band ratios, can provide a rapid, low-preparation laboratory-based approach for estimating capsicum leaf water traits under controlled dehydration, supporting plant-based water stress assessment under controlled conditions and providing a basis for further irrigation-related sensing studies. However, the models are preliminary and require validation with larger independent datasets and tightly standardised measurement conditions before operational use in irrigation management.
Why it matches plant phenotyping methodsMIR ATR-FTIRスペクトルと選択バンド比を用いて、葉の水分形質を推定するセンシング・予測手法の開発と性能評価が中心である。
abstractHere, practical prediction models were developed using mid-infrared (MIR) ATR-FTIR spectra of capsicum (Capsicum annuum L.) leaves
Early detection of chili plant diseases is essential for preventing yield loss, yet practical deployment remains challenging due to inconsistent illumination, leaf orientation variability, and the limited computational capacity of low-cost imaging hardware. This study proposes an integrated detection framework combining an ESP32-CAM acquisition pipeline, MQTT-based transmission, and a Python inference engine running a fine-tuned ResNet-18 model optimized for real-world noise conditions. The research aims to determine whether domain-aligned fine-tuning meaningfully improves generalization performance compared to older non-optimized models under field-like variability. Using a four-day observational design with two leaf subsets, the fine-tuned models consistently outperformed their non-fine-tuned counterparts in overall accuracy, per-class stability, and positional robustness. Real-time deployment using the Telegram Bot API successfully delivered classification results and images with low latency, demonstrating operational feasibility for remote plant health monitoring. These findings indicate that targeted fine-tuning is essential for transforming CNN-based classifiers from laboratory prototypes into stable, field-ready systems capable of supporting early disease detection in resource-constrained agricultural environments. Additionally, due to chili is widely cultivated in agroforestry systems in Indonesia, the proposed early disease detection framework offers substantial benefits for maintaining productivity in heterogeneous microclimatic conditions where manual diagnosis is more difficult.
Why it matches plant phenotyping methodsチリ葉の病害状態を画像から分類するCNNベースの取得・推論フレームワークを開発し、非最適化モデルとの性能比較と実環境展開を検証しており、植物フェノタイピング手法が中心である。
abstractThis study proposes an integrated detection framework combining an ESP32-CAM acquisition pipeline, MQTT-based transmission, and a Python inference engine running a fine-tuned ResNet-18 model optimized for real-world noise conditions.
Xylem tissue enables efficient long-distance water transport but is a primary target for vascular pathogens. This study investigates how systemic invasion by Verticillium dahliae impairs the hydraulic function of pepper (Capsicum annuum) plants, focussing on xylem colonisation and its anatomical and physiological effects. Real-time sap flow was continuously monitored with custom-built ExoBeat sensors, while periodic stem water potential measurements allowed calculation of changes in stem hydraulic conductance as an additional indicator of xylem performance. Fungal colonisation was assessed by quantitative polymerase chain reaction, and vessel occlusions and embolised conduits were visualised using scanning electron microscopy and micro-computed tomography, complemented by direct hydraulic conductivity measurements. By 14 d post inoculation, V. dahliae had progressed from roots to aboveground tissues, coinciding with a marked decrease in sap flow, water potential, and soil-to-stem hydraulic conductance, alongside the onset of dwarfing. Direct fungal blockage and anatomical changes were the primary contributors to hydraulic dysfunction. Vessel occlusion by tyloses, gels, and air embolisms played a negligible role. This study reveals how V. dahliae progressively impairs pepper hydraulics through systemic xylem colonisation, highlighting the value of real-time sap flow monitoring. Our integrative, multidisciplinary approach offers a powerful framework to unravel the complexity of dynamic plant-fungal vascular interactions.
Why it matches plant phenotyping methodsカスタムセンサーによるリアルタイム・サップフロー測定を中心に、植物の水理機能・病原体による機能低下を定量化しており、単なる生物学的測定にとどまらない実質的なフェノタイピング手法の適用である。
abstractReal-time sap flow was continuously monitored with custom-built ExoBeat sensors
An early and precise identification of plant diseases helps to increase the efficiency of farming operations and minimize the economic losses associated with plant diseases. Nevertheless, applying deep learning models for plant disease identification in an agricultural setting poses certain difficulties due to high computational costs and insufficient edge device computing power. This paper presents a transfer learning-based framework for multi-class plant disease detection using MobileNetV2 and EfficientNet-B0 models. For the purpose of research, the PlantVillage dataset including potato, bell pepper, and tomato leaves was used. Images from this source underwent pre-processing that included resizing, normalizing, and augmenting images. Both transfer learning approach and fine-tuning helped to modify pre-trained CNNs for multi-class classification of different diseases affecting plants' leaves. Experiments have shown that EfficientNet-B0 model performed much better with accuracy of 95.7% and AUC of 0.98. Moreover, the proposed algorithm was exported as a TensorFlow Lite model and implemented in the Streamlit application for efficient edge deployment.
Why it matches plant phenotyping methods植物葉画像から病害を推定する深層学習フレームワークの開発・性能評価が研究の中心であり、植物の病害状態を直接推定するため、植物フェノタイピング手法として採録する。
abstractThis paper presents a transfer learning-based framework for multi-class plant disease detection using MobileNetV2 and EfficientNet-B0 models.
Plant phenotyping relevance match · UnverifiedOpenAlex · Crossref · Europe PMC · checked 5 Sept 2026
Quantitative phenotyping of pepper seedlings is important for greenhouse plug tray seedling cultivation, but it remains constrained by inefficient manual monitoring, complex greenhouse backgrounds, and growth-stage-dependent discrepancies between two-dimensional image traits and actual leaf biomass. In this study, a cascaded vision framework with stage-specific morphological correction was developed for nondestructive seedling phenotyping. The framework integrated Visual Dynamic Momentum YOLO (VDM-YOLO) for individual seedling localization and growth-stage recognition, Variance Guided Strip Ghost Gated UNet (VSG-UNet) for lightweight, high-resolution leaf segmentation, and a stage-aware correction model for leaf dry biomass estimation. In performance evaluation, VDM-YOLO achieved a mean average precision at an intersection over union threshold of 0.5 (mAP0.5) of 89.27%, improving mAP0.5 by 1.82 percentage points over YOLOv12. VSG-UNet achieved a mean intersection over union (mIoU) of 83.9% and a Dice coefficient of 81.8%, while reducing floating point operations (FLOPs) and parameters by 44.2% and 61.2%, respectively, compared with U-Net. After stage-aware calibration, the coefficient of determination (R2) between segmented area and leaf dry weight increased from 0.764 to 0.813, and the root mean square error (RMSE) decreased from 0.0210 g to 0.0190 g. These results demonstrated that the proposed framework provided a proof of concept approach based on RGB images for the nondestructive assessment of leaf area and leaf dry biomass in pepper seedlings under restricted experimental conditions.
Why it matches plant phenotyping methodsRGB画像による葉の検出・セグメンテーションと、葉面積から葉乾燥バイオマスを推定する手法を開発・評価しており、植物表現型取得が研究の中心である。
abstracta cascaded vision framework with stage-specific morphological correction was developed for nondestructive seedling phenotyping.
ToBRFV is a major threat to tomato and pepper crops because it spreads quickly and survives for a long time in the environment. Since there are few ways to control it after infection, early detection before symptoms are visible is crucial. Yet, only limited public datasets are available for this research. We present one of the first openly accessible, longitudinal multispectral image dataset dedicated to ToBRFV detection. In this study, two tomato cultivars and two pepper cultivars, all of which are commercially important and widely cultivated in greenhouses, were selected. Using these plants ensures that the dataset reflects real-world agricultural practices and captures variability across commercially grown types. Both healthy and ToBRFV-inoculated plants from each cultivar were included in the imaging process. All plants were cultivated under fully controlled greenhouse conditions in Adana Province, Türkiye. Healthy and infected tomato plants were grown in two separate greenhouses to prevent cross-contamination. Imaging was conducted over a 29-day period using Red-Green-Blue (RGB) and Visible Near Infrared (VNIR) cameras, including narrowband captures at 800 nm and 1000 nm, from multiple viewing angles. Infection status was confirmed via Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) analysis at multiple time points. The dataset is organized into four clean, labelled subsets and released under a CC BY 4.0 license. This resource provides unique opportunities for developing and benchmarking computer vision and machine learning approaches for pre-symptomatic plant disease detection, spectral feature analysis, and integration into precision agriculture systems. By combining controlled experimental design, spectral diversity, and open access, it establishes a robust foundation for cross-disciplinary research in plant pathology, agricultural engineering, and artificial intelligence.
Why it matches plant phenotyping methods植物病害状態を対象にした縦断マルチスペクトル画像データセットであり、公開データセットとして開発・ベンチマーク利用を目的とするため、表現型取得が中心です。
abstractWe present one of the first openly accessible, longitudinal multispectral image dataset dedicated to ToBRFV detection.
Reproduction assets foundThe article is a Data in Brief describing the authors' own openly released longitudinal multispectral plant image dataset (ToBRFV-LMID) for tomato and pepper disease detection, deposited on Zenodo under CC BY 4.0 with a direct DOI URL. This is a paper-specific, public, directly actionable phenotype/image asset. No codeDataset · publicData accessibility
Repository name: ZENODO
Data identification number: 10.5281/zenodo.17244968
Direct URL to data: https://doi.org/10.5281/zenodo.17244968Open asset ↗ZENODO · 10.5281/zenodo.17244968html-lines:98-126Plant phenotyping relevance match · UnverifiedCrossref · OpenAlex · checked 15 Sept 2026
High-quality, real-time three-dimensional (3D) reconstruction and phenotyping of field crops are essential for advancing digital farm management. However, challenges such as diverse crop postures and fluctuating illumination in open-field environments significantly hinder reconstruction accuracy. To address these issues, this study proposes AgriGaussian, a novel 3D phenotyping method based on 3D Gaussian Splatting (3DGS), designed to enable low-cost, high-fidelity reconstruction and phenotypic analysis of agricultural fields, with chili pepper plants as an example. Specifically, a dataset encompassing three distinct growth stages of chili pepper plants was collected and preprocessed using a phenotyping robot (PR) equipped with oblique-view imaging. The AgriGaussian method integrates three core components: (1) a Depth-Supervised Strategy (DSS) for adaptively enhancing phenotypic features based on gradient variations; (2) a Crop Appearance Extraction (CAE) module that suppresses the effects of illumination and environmental interference while preserving fine phenotypic details; and (3) a Multi-scale Chunking Training (MCT) strategy that enables scalable reconstruction of large agricultural scenes. Experimental results demonstrate that AgriGaussian achieves high-fidelity reconstruction, with peak signal-to-noise ratio (PSNR) of 26.85 dB, structural similarity index (SSIM) of 0.86, and learned perceptual image patch similarity (LPIPS) of 0.24—surpassing existing baseline algorithms. Compared to manual measurements, the reconstructed canopy height and volume exhibit strong agreement, with coefficients of determination (R2) reaching 0.90 and 0.86, respectively, and average absolute percentage errors as low as 5.1%. Furthermore, the method accurately reconstructs fine-grained features such as foliar lesions and surface textures. In summary, AgriGaussian enables large-scale, high-fidelity reconstruction of field crop phenotypes and provides a promising foundation for advancing 3D phenotyping and digital twin technologies in precision agriculture.
Why it matches plant phenotyping methods植物の3D形態・構造を抽出する低コスト画像ベース表現型解析法を開発し、手動測定との一致性も検証しているため、表現型取得法が研究の中心である。
abstractthis study proposes AgriGaussian, a novel 3D phenotyping method based on 3D Gaussian Splatting (3DGS)
Burkina Faso's agriculture sector faces major challenges, with annual crop losses reaching 40% due to plant diseases, affecting 2.7 million people in a situation of food insecurity. This research presents an innovative automatic plant disease detection system using computer vision, specifically developed for West African constraints. The system is based on the YOLOv11 (You Only Look Once) unified detection architecture, recognised for its optimal balance between speed and accuracy in real time, essential for mobile deployment to detect diseases in maize, tomatoes and chillies with an overall accuracy of ~99%. The image dataset from Kaggle has been validated by local agronomic expertise from INERA, ensuring the relevance of disease classes specific to the Sahelian context. The proposed architecture demonstrates superior performance to existing approaches while being optimised for mobile deployment. This solution contributes to the development of decision support tools for precision agriculture in West Africa.
Why it matches plant phenotyping methods葉画像から植物病害を自動検出するコンピュータビジョン手法の開発が中心で、植物の病害状態を直接推定しているため、植物フェノタイピング手法として採用。
abstractThis research presents an innovative automatic plant disease detection system using computer vision
Optimizing crop yield while minimizing energy consumption remains a central challenge in greenhouse horticulture. This study introduces an integrated deep learning framework that couples multi-horizon time-series forecasting with dual-layered explainability to address the critical need for spatiotemporal transparency in optimizing greenhouse crop yield and energy efficiency. Four deep learning architectures, including the One-Dimensional Convolutional Neural Network (1D-CNN), Long Short-Term Memory Network (LSTM), Bidirectional Long Short-Term Memory Network (BiLSTM), and TinyTimeMixer (TTM), were evaluated across two varieties of capsicum. LSTM and BiLSTM achieved the highest accuracy for incremental yield prediction, whereas TTM outperformed other models in forecasting daily energy usage, reflecting the distinct temporal characteristics of biological growth and environment-driven energy demand. To uncover the factors driving these predictions, two complementary explainability methods were applied: Gradient SHapley Additive exPlanations (SHAP) for feature-level attribution and a Temporal Convolutional Network with Convolutional Block Attention Module (TCN–CBAM) attention mechanism for joint temporal-feature interpretation. Radiation and drainage-related variables consistently emerged as the dominant contributors to yield, whereas external temperature, and humidity were the primary determinants of energy usage. Temporal attention further showed that yield is influenced by both recent irrigation responses and longer-term developmental dynamics, while energy consumption is driven mainly by short-term climatic fluctuations. These findings provide actionable insights for irrigation scheduling, climate-control strategies, and energy optimization, supporting more transparent and sustainable greenhouse management.
Why it matches plant phenotyping methods温室作物の収量という植物形質を深層学習で予測し、複数モデル比較と説明可能性解析を行う計算的形質推定手法が研究の中心である。
abstractThis study introduces an integrated deep learning framework that couples multi-horizon time-series forecasting with dual-layered explainability
Chili peppers (Capsicum annuum L.) are a strategic horticultural commodity in Indonesia, but their productivity is often hampered by pathogen infections that cause leaf diseases such as anthracnose, leaf spot, and yellow virus. Early detection by farmers is still dominated by subjective visual observation and prone to misdiagnosis due to the similarity of symptoms between diseases. Although Deep Learning technology through Convolutional Neural Networks (CNN) offers an automated solution, implementation in real-world conditions still faces significant challenges such as lighting variations, complex backgrounds, and limited local datasets. This often leads to a drastic decrease in model performance compared to testing in a controlled environment. To address these issues, this study proposes an optimization of the transfer learning strategy on the MobileNetV2 architecture by integrating progressive layer-wise fine-tuning and adaptive data augmentation techniques. The fine-tuning method is carried out gradually on the pre-trained model layers, while adaptive augmentation dynamically manipulates images based on environmental characteristics to improve model robustness. The results of this study, which include multi-class classification on cross-location image data, are projected to be able to boost the accuracy and generalization ability of the model in heterogeneous field conditions. Practically, this research provides a framework for a more precise and robust disease detection system to accelerate the implementation of precision agriculture in the future.
Why it matches plant phenotyping methods植物葉の病徴を画像から分類するCNN手法の改良が研究の中心であり、病害状態の表現型推定に該当する。転移学習のファインチューニングと適応的画像拡張、異なる圃場条件での頑健性評価を扱っている。
abstractThe results of this study, which include multi-class classification on cross-location image data, are projected to be able to boost the accuracy and generalization ability of the model in heterogeneous field conditions.
Reproduction assets foundThe paper states its chili leaf image dataset was supplemented with data from a supporting repository, cited as a public Mendeley Data deposit (reference [2]). This is a public plant-image dataset directly used for the paper's disease-classification phenotyping. No authors' analysis code or trained model checkpoint is,Dataset · public[2] F. Wajidi and N. Arifin, “Deteksi Penyakit Daun Cabai Menggunakan Kombinasi GLCM dan HSV dengan
Klasifikasi SVM,” vol. 11, no. 02, 2025. [Online]. Available:
https://data.mendeley.com/datasets/w9mr3vf56s/1Open asset ↗w9mr3vf56s/1pdf-page:9 lines:1-56Plant phenotyping relevance match · UnverifiedCrossref · checked 14 Sept 2026
Published19 May 2026International Journal on Advanced Computer Theory and EngineeringCited by 0 · OpenAlex ↗
Agriculture constitutes a foundational pillar of the Indian economy, yet crop diseases remain one of the most persistent threats to agricultural productivity, particularly for smallholder farmers who lack immediate access to plant pathology expertise. To bridge this critical gap, the present work proposes Smart Crop Doctor, an intelligent web-based platform that leverages Artificial Intelligence to perform automated detection of crop diseases. Within this framework, users submit photographs of plant foliage, which are subsequently analyzed by a trained Convolutional Neural Network (CNN) capable of recognizing pathological conditions across 15 distinct disease categories spanning tomato, potato, and bell pepper cultivars. A dedicated input validation mechanism is incorporated to ascertain whether a submitted photograph genuinely depicts leaf tissue, thereby filtering out extraneous objects such as rocks or paper-based documents. Upon successful identification, the platform furnishes comprehensive output including disease characterization, recommended treatment protocols, and guidance on both organic and chemical fertilizer application, in addition to broader agronomic advisory content. Beyond disease diagnosis, the system integrates a suite of ancillary services: a%, confirming that the system delivers dependable performance suited to practical deployment in agricultural settings.
Why it matches plant phenotyping methods植物葉の画像から病害状態をCNNで推定する診断システムが研究の中心であり、植物病害フェノタイピング手法・プラットフォームに該当する。
abstractthe present work proposes Smart Crop Doctor, an intelligent web-based platform that leverages Artificial Intelligence to perform automated detection of crop diseases.
This study developed a model to predict zeaxanthin content in peppers using multispectral imaging and chemical data. A one-dimensional convolutional neural network (1D CNN) model was identified as the optimal single-modal model after comparing four machine learning algorithms. On the prediction dataset, the model achieved a determination coefficient ( Rp 2 ) of 0.7639. Building upon the 1D CNN framework, a multimodal feature fusion model (MCSF) was constructed by integrating the chemical measurements of capsanthin and total carotenoid contents using a multilayer perceptron. This enhanced model demonstrated excellent predictive accuracy and robustness, with Rp 2 values of 0.9318 and 0.9211 across different spectral ranges. For high-throughput detection purposes, a simplified model that replaced measured capsanthin with a comprehensive red index still performed well, with an Rp 2 of 0.8912 and an RPD of 3.11. This strategy provides a new solution for the efficient spectral detection of plant chemicals affected by multicollinearity in their absorption spectra.
Why it matches plant phenotyping methodsマルチスペクトル画像と機械学習を用いて、トウガラシ果皮のゼアキサンチン含量という植物器官の形質を非破壊・高スループット推定する手法を開発・評価しており、フェノタイピング手法が中心である。
abstractThis study developed a model to predict zeaxanthin content in peppers using multispectral imaging and chemical data.
Reproduction assets foundThe paper's data availability statement explicitly states that the datasets (multispectral imaging and chemical trait measurements) and the main model code are publicly available in the authors' GitHub repository, which is an allowed URL.Dataset · publicThe datasets and the main model code are available online at https://github.com/liang-wei-tian/Chili-Peppers-Zeaxanthin.Open asset ↗liang-wei-tian/Chili-Peppers-Zeaxanthinhtml-lines:303-325Plant phenotyping relevance match · UnverifiedEurope PMC · checked 5 Sept 2026
Chili pepper (Capsicum spp.) is a high-value industrial horticultural crop widely utilized in food processing, pharmaceuticals, and natural pigment production. Accurate monitoring of flowering is critical for yield formation, pollination management, and early-stage production forecasting in industrial chili production systems. However, in greenhouse environments, chili flowers typically exhibit small object scale and are affected by issues such as lighting variations and occlusion, which pose significant challenges for reliable visual detection. These factors often result in missed detections and unstable performance in practical phenological monitoring tasks. To address these challenges, this study proposes CF-DETR, a robust transformer-based framework for small-scale chili flower detection. Built upon the RT-DETR architecture, the proposed method introduces an efficiency-optimized FasterNet backbone to enhance fine-grained feature extraction for small targets while maintaining computational efficiency. In addition, a dynamic upsampling mechanism is incorporated to preserve structural details during feature reconstruction, and a Bidirectional Multi-scale Attention Feature Pyramid Network (BiMAFPN) is designed to strengthen cross-scale feature interaction under complex greenhouse backgrounds and occlusion conditions. Experiments conducted on a self-constructed greenhouse dataset demonstrate that CF-DETR achieves a Precision of 94.1%, mAP50 of 83.5%, and mAP50-95 of 64.5%, outperforming the baseline RT-DETR-r18 model. Furthermore, deployment on an NVIDIA Jetson AGX Orin platform achieves real-time inference at 30.65 FPS, validating its practical applicability in edge-enabled agricultural systems. The proposed framework provides a reliable visual sensing solution for small-scale phenology monitoring, enabling intelligent pollination management, early yield prediction, and data-driven decision-making in industrial chili production. This work contributes to the advancement of precision horticulture and the digital transformation of industrial crop production systems.
Why it matches plant phenotyping methodsチリ花の検出を目的とする画像ベースの手法を開発し、データセット上で性能評価とエッジ実装検証を行っており、植物の開花状態を取得する方法が中心である。
abstractthis study proposes CF-DETR, a robust transformer-based framework for small-scale chili flower detection.
Accurate pixel-level delineation of crop disease in greenhouse images is challenging due to weak lesion margins, scale variations, leaf-vein interference, specular highlights and partial occlusion. This paper introduces a boundary-refined DeepLabV3+ model that keeps the encoder-decoder efficiency of the original framework and adds a boundary supervision branch, uncertainty-aware cross-scale fusion, and an adaptive refinement gate. The network is tested on a set of 6,840 curated greenhouse images that include leaves of tomatoes, cucumbers, peppers and eggplants, as well as 8 disease categories and healthy tissue. Under a fixed split by greenhouse compartment, the proposed model has achieved a mean intersection over union of 89.7%, a mean F1 score of 94.8%, and a boundary F1 of 86.9% at a two-pixel tolerance. The corresponding values are 3.8, 2.4 and 7.6 percentage points higher than those of the standard DeepLabV3+. The mean Intersection over Union (IoU) under low-illumination and condensation-blur conditions are 3.1 and 2.7, respectively. Ablation studies show that boundary supervision is responsible for most of the contour improvement, and uncertainty-aware fusion reduces false lesion expansion along veins. The model has 31.6 million parameters and, after mixed-precision optimisation, runs at 18.7 frames per second on an embedded graphics chip. Based on the above results, explicit boundary reasoning can improve the precision of disease-area estimation without sacrificing the efficiency required in practice; it is thus suitable for greenhouse scouting, targeted spraying and longitudinal severity assessment.
Why it matches plant phenotyping methods温室画像から作物病斑の画素レベル境界と病害面積を推定する画像解析手法を開発・検証しており、植物病害状態の表現型取得が中心である。
abstractThis paper introduces a boundary-refined DeepLabV3+ model that keeps the encoder-decoder efficiency of the original framework and adds a boundary supervision branch, uncertainty-aware cross-scale fusion, and an adaptive refinement gate.
ABSTRACT Smart agriculture based on the use of Artificial Intelligence for crop disease detection to ensure food security. Fungal disease is one of the major causes that affects the quality of the vegetables. Convolutional neural networks (CNNs) and vision transformers (ViTs) enable the detection of crop diseases at an early stage, allowing farmers to take preventive measures and minimize further losses. The proposed method introduces an ensemble of Custom CNN to understand multilevel local features and Pretrained ViT to capture global dependencies as well as contextual information from the dataset. An interactive cross attention module (ICAM) facilitates bidirectional information exchange between CNN and transformer token representations, while an attention guided gated fusion (AGGF) mechanism adaptively combines complementary features. The Indian Crop Visual Disease Dataset (ICVDD‐5) has been developed in a real field for the proposed work with the help of domain experts. The dataset contains 880 diverse images depicting both healthy and diseased specimens of five vegetable crops. The crops selected for this research initiative include Brinjal, Cabbage, Chili, Okra, and Tomato. These five crops are examined for about 21 distinct disease classes. Comprehensive ablation studies are conducted to prove the contributions of each architectural component, including CNN‐only, ICAM‐disabled, and AGGF‐disabled configurations. Experimental results demonstrate that the proposed ICAG‐Net achieves a test accuracy of approximately 70%–73% with improved macro‐F1 score compared to baseline CNN models under identical training settings. The novelty of this work lies in an extensible solution for real world crop disease diagnosis systems and offers insights into hybrid CNN–transformer architectures for small scale agricultural datasets.
Why it matches plant phenotyping methodsCNN・Transformerによる植物病害症状の画像検出手法を開発し、実圃場画像データセットの構築、アブレーション、ベースライン比較で性能検証しているため、植物フェノタイピング手法が中心である。
abstractThe proposed method introduces an ensemble of Custom CNN to understand multilevel local features and Pretrained ViT to capture global dependencies as well as contextual information from the dataset.
Plant diseases are a major problem for farmers around the world, reducing crop yields. The absence of expertise makes plant disease detection difficult and complicated. Plant disease detection is made easier by deep learning algorithms; however, they are computationally demanding and need huge training datasets. This research work proposes a novel Conv-7 DCNN model with modified ParNet attention layer to classify plant leaves into distinct categories with improved accuracy. Because of its architecture, the proposed network can identify leaf diseases with more accuracy by extracting the wider range of features from the images. The proposed Conv-7 DCNN model classifies the leaf diseases of three plants such as tomato, potato, and pepper-bell into fifteen categories. The CNN model is trained using publicly accessible Kaggle dataset, utilising image augmentation techniques. It is evident from the simulation results that the proposed model outperforms several pre-trained, and other trending deep learning models. Proposed model achieves 99.18% classification accuracy with an average precision of 99.17% and area under the curve (AUC) of 1, making this model highly effective in leaf diseases detection. Additionally, Conv-7 DCNN achieved high FPS of 112.49, low inference time of 18.34 s, and low GFLOPS of 13.98, making it suitable for real-time applications in smart agriculture systems.
Why it matches plant phenotyping methods植物葉画像から病害状態を推定する深層学習モデルを開発・比較しており、病害表現型の取得・分類手法が研究の中心である。
abstractThis research work proposes a novel Conv-7 DCNN model with modified ParNet attention layer to classify plant leaves into distinct categories with improved accuracy.
Reproduction assets foundThe paper's plant-phenotyping measurements (leaf disease classification of tomato, potato, and pepper-bell) are based on a publicly available Kaggle dataset explicitly named in the Data Availability statement. No author analysis code, trained model checkpoints, or other paper-specific assets are disclosed.Dataset · publicThe dataset is available online at https://www.kaggle.com/datasets/emmarex/plantdisease.Open asset ↗Kaggle · emmarex/plantdiseasehtml-lines:824-854Plant phenotyping relevance match · UnverifiedOpenAlex · Europe PMC · checked 5 Sept 2026
Recent technological advancements employ imaging techniques to examine the morphological, physiological, and genetic differences among plant accessions, enhancing precision and productivity. High-throughput phenotyping serves as an essential method for selecting traits and reducing errors tied to manual data collection. However, the effects of camera-to-object distance in imaging acquisition for plant phenomics have received less attention. We analyzed the imaging parameters that define well the morphological characteristics of pepper and the effects of camera-to-object distance on the imaging of plant growth, leaf dimensions, fruit, and seed characteristics. Three camera-to-object distances (0.8, 1.0, and 1.2 m) were studied for the vegetative stage, and six camera-to-object distances (0.35-0.85 m) were used for the reproductive stages. The results demonstrated that imaging parameters such as Major (the longest line that can be drawn within the leaf) and Minor (the shortest line perpendicular to the major axis) are more effective for assessing canopy spread, while imaging Height provides a strong correlation (r = 0.9) for actual plant height measurement. An optimal camera-to-object distance of 0.8 m yielded better correlations for vegetative traits across all pepper genotypes, likely due to resolution factors at different growth stages. For fruit and seed traits, shorter distances of 0.55 m and 0.65 m were suitable. Additionally, the weights of fresh and dry fruit correlated highly with image area (r = 0.94 and 0.89, respectively, at 0.55 m). The studied pepper genotypes exhibited distinct seed characteristics, including variations in Roundness, Solidity, and Circularity. The imaging approach can accurately capture various plant characteristics and has the potential to replace traditional methods for assessing plants.
Why it matches plant phenotyping methodsRGB画像による植物形質取得を中心に、カメラ距離と撮像パラメータを最適化・検証しており、方法開発および技術検証に該当する。
titleDigitalised phenotyping of pepper (Capsicum spp.) using effective RGB imaging and optimised camera positioning.
Plant phenotyping relevance match · UnverifiedCrossref · Europe PMC · checked 5 Sept 2026
Pepper / chilliTomatoGreenhouseFruitClassificationSegmentationGrowth / development / phenology
Accurate classification of fruit maturity is essential for automated grading and robotic manipulation in modern greenhouse cultivation. Most existing methods rely on crop-specific models, severely restricting their scalability in multi-crop scenarios. To overcome this limitation, this study presents SCEA-YOLO, a unified and efficient instance segmentation framework built on YOLOv11s-seg, for simultaneous maturity classification of tomatoes and sweet peppers. To boost feature discrimination, reduce computational redundancy, and alleviate class imbalance, SCEA-YOLO integrates spatial-channel reconstruction convolution and an efficient multi-scale attention mechanism, while replacing the original detection head with the proposed EA-Head. The model is evaluated on a hybrid dataset captured under diverse greenhouse conditions, including varying illumination, fruit occlusion, and overlapping canopies. Its robustness to different viewing angles and camera distances is further validated via deployment on an automated grading robot. Compared with the baseline, SCEA-YOLO enhances classification precision and mAP50–95 by 5.3% and 2.3% for tomatoes, and 1.2% and 1.4% for sweet peppers, respectively. With only 33.2 GFLOPs, the model satisfies real-time inference demands. Benefiting from its lightweight structure and real-time performance, SCEA-YOLO can be readily deployed on embedded systems and robotic platforms. It offers a practical, unified, and scalable solution for intelligent fruit maturity evaluation in multi-crop greenhouse production.
Why it matches plant phenotyping methodsトマトとピーマン果実の成熟度を画像から分類・評価するモデルを開発し、データセットおよびロボット上で性能検証しており、植物表現型取得手法が中心である。
abstractthis study presents SCEA-YOLO, a unified and efficient instance segmentation framework built on YOLOv11s-seg, for simultaneous maturity classification of tomatoes and sweet peppers.
Crop disease detection is critical for agricultural productivity and global food security. Traditional methods rely on labour-intensive field surveys prone to human error. This paper presents an image-based crop disease detection system using a hybrid Convolutional Neural Network (CNN) augmented with pre-trained AlexNet weights. The system captures leaf images, applies preprocessing (resizing, normalization, augmentation), and classifies diseases with high accuracy. A Flask-based web application enables real-time prediction accessible to farmers via smartphone. Trained on 2,000 field-collected images across potato, pepper, and tomato crops, the proposed model achieves approximately 97% classification accuracy, outperforming standalone classifiers including SVM, Logistic Regression, Decision Tree, and Naïve Bayes
Why it matches plant phenotyping methods葉画像から植物病害を推定する画像ベース手法の開発・比較検証が研究の中心であり、植物の病害状態を直接評価しているため。
abstractThis paper presents an image-based crop disease detection system using a hybrid Convolutional Neural Network (CNN) augmented with pre-trained AlexNet weights.
施設園芸における環境制御では,温度や湿度の空間変動など環境の不均一性を考慮せずに,平均化された指標に基づいた制御が行われており,作物の生育にばらつきが生じる問題があった.これらを解決するためには,主に日照や温度・湿度などの不均一の原因となっている作物群落のキャノピー構造を可視化することが重要である.本研究では,低コストの2D LiDAR(Light Detection and Ranging)計測により,作物のキャノピー構造の可視化を試み,薄い葉や細い茎によるレーザー反射の有効性と適切なスキャン条件を検証した.機器を設置した台車を移動プラットフォームとして,圃場の畝に沿って移動させることで,畝に沿った作物のキャノピー構造を把握する.2つのLiDARの走査面を変えて用いることとし,水平スキャンにより,台車進行方向の作物及び障害物の2Dマッピング,垂直スキャンにより作物の高さ方向のスキャンを時系列的に重ねることで,3Dマッピングを行う.結果として,水平スキャンのデータは,台車の走行制御のための状況把握としては十分な精度で利用可能である.垂直スキャンのデータは,作物の高さ方向の構造を把握できることが確認された.2つのLiDARを搭載したシステムを用いて,圃場で定期的に移動計測を行うことで,作物のキャノピー構造を把握することができ,環境の不均一の要因として利用可能となることが期待される.
Why it matches plant phenotyping methods低コスト2D LiDARによる作物キャノピー構造の可視化手法を開発し、反射の有効性と走査条件を検証しているため、植物表現型取得が研究の中心である。
abstract本研究では,低コストの2D LiDAR(Light Detection and Ranging)計測により,作物のキャノピー構造の可視化を試み,薄い葉や細い茎によるレーザー反射の有効性と適切なスキャン条件を検証した.
Modern precision agriculture requires the incorporation of high-accuracy diagnostic instruments to guarantee food security for inexperienced practitioners. This paper introduces an AI-driven agricultural web architecture that connects deep learning-based diagnostics with real-world farm management. The main contribution is a Convolutional Neural Network (CNN) framework that can automatically find diseases in five common crops: Capsicum annuum, Vitis vinifera, Zea mays, Solanum tuberosum, and Solanum lycopersicum. The proposed model reached a final training accuracy of 98.30% and a validation accuracy of 90.12% over 10 epochs by using a sequential architecture with optimized convolutional layers and data augmentation. The platform has a localized marketplace, a government scheme eligibility engine, and a Crop Journal for long-term record-keeping to make it useful in the real world. Results demonstrate that this unified ecosystem provides a transparent and accessible framework for data-informed agricultural management, effectively lowering the technical barrier for new farmers.
Why it matches plant phenotyping methodsCNNによる作物病害の自動検出が中心的な技術貢献であり、植物の病害状態を画像ベースで推定するため、農業サービス部分を含んでも植物フェノタイピング手法として採用する。
abstractThis paper introduces an AI-driven agricultural web architecture that connects deep learning-based diagnostics with real-world farm management.
Accurate fresh weight (FW) estimation is essential for growth monitoring and yield prediction in greenhouse fruit vegetables, but remains challenging due to the dynamic allocation between vegetative and reproductive organs. This study aimed to systematically evaluate modeling strategies for FW estimation in sweet pepper and identify which approach is most suitable under conditions of dynamic biomass partitioning. Non-destructive morphological measurements were collected under greenhouse cultivation, and allometric models based on geometric equations were established as baselines. Their performance was compared with machine learning (ML) models and ensemble learning frameworks. To address limited data availability, numerical data augmentation with Gaussian noise and a variational autoencoder was applied. Among the allometric models, the stick model combined with a sigmoid function showed the highest performance, with an R2 of 0.80 for shoot FW and 0.54 for fruit FW. All ML models outperformed the allometric models, and the ensemble model achieved the highest predictive accuracy, with an R2 of 0.96 for shoot FW and 0.89 for fruit FW. Data augmentation further improved predictive performance across all ML models, particularly for fruit FW prediction. Feature contribution analysis revealed that temporal progression was the dominant predictor of fruit FW, while structural traits played the primary role in shoot FW estimation. Ensemble-based ML, combined with data augmentation, provides a methodological framework for non-destructive FW estimation of sweet pepper in controlled environments such as greenhouses and smart farming systems.
Why it matches plant phenotyping methods非破壊形態計測からピーマンの地上部・果実の生重を推定するアロメトリック/機械学習/アンサンブル手法を比較・評価しており、表現型取得・推定手法が研究の中心である。
abstractThis study aimed to systematically evaluate modeling strategies for FW estimation in sweet pepper
Early and precise detection of plant diseases is essential for safeguarding crop yield and ensuring sustainable agricultural practices. In this study, we propose the Modified RefineNet with Attention based Fusion (MoRefNet-AF), a Modified RefineNet architecture enhanced with attention-based fusion for multi-class classification of corn (maize) and Pepper leaf diseases. Unlike the original RefineNet, which was segmentation-oriented and computationally heavy, MoRefNet-AF is redesigned for lightweight and discriminative classification. The modifications include replacing standard convolutions with depthwise separable convolutions for efficiency, adopting the Mish activation function for smoother gradient flow, redesigning the multi-resolution fusion module with concatenation and shared convolution for richer cross-scale integration, and incorporating Squeeze-and-Excitation (SE) blocks for adaptive channel recalibration. Additionally, Chained Residual Pooling (CRP) with atrous convolutions enhances contextual representation, while global average pooling with dense layers improves classification readiness. When evaluated on a curated six-class dataset combining PlantVillage and Mendeley leaf disease repositories, MoRefNet-AF achieved 99.88% accuracy, 99.74% precision, 99.73% recall, 99.95% F1-score, and 99.73% specificity. These results outperform strong baselines including ResNet152V2, DenseNet201, EfficientNet-B0, and ConvNeXt-Tiny, while maintaining only 0.3 M parameters. With its compact design and TensorFlow Lite (v2.13) compatibility, MoRefNet-AF offers a robust, lightweight, and real-time deployable solution for precision agriculture and smart plant disease monitoring.
Why it matches plant phenotyping methods植物葉の病徴を画像から分類する軽量深層学習手法を開発し、複数データセットとベースラインで性能評価しているため、植物フェノタイピング手法が中心である。
abstractwe propose the Modified RefineNet with Attention based Fusion (MoRefNet-AF), a Modified RefineNet architecture enhanced with attention-based fusion for multi-class classification of corn (maize) and Pepper leaf diseases.
Plant diseases pose a significant threat to global agriculture, impacting crop yields and quality. Early and accurate detection is essential for effective health management but remains challenging due to visual similarity among diseases and complex field backgrounds. This study introduces AgriMM, a novel multi-modal detection framework that integrates visual images with expert-validated textual descriptions to improve diagnostic precision. The framework features three key innovations: a Hybrid Convolutional-Attention Collaborative Backbone (HCACB) to capture both fine-grained lesions and global context; a Context-enhanced Visual-Language Path Aggregation Network (CVL-PAN) for multi-scale feature fusion; and an Adaptive Region-Text Contrastive Learning (AR-TCL) module to enforce precise semantic alignment. We constructed a comprehensive dataset comprising 30,000 images and detailed symptom descriptions across five major crops (tomato, cucumber, pepper, eggplant, and squash). Experimental results demonstrate that AgriMM achieves a mean Average Precision (mAP) of 95.2%, significantly outperforming state-of-the-art unimodal baselines by 11.6%. These findings confirm that integrating linguistic semantic priors effectively resolves visual ambiguity, providing a robust tool for precision agriculture and sustainable crop protection.
Why it matches plant phenotyping methods植物病害の症状を画像から検出・診断するマルチモーダル手法を開発し、データセットと性能比較で検証しているため、植物フェノタイピング手法が中心である。
abstractThis study introduces AgriMM, a novel multi-modal detection framework that integrates visual images with expert-validated textual descriptions to improve diagnostic precision.
Abstract Background Breeding for cold tolerance in pepper ( Capsicum annuum L.) is critical to mitigate yield losses caused by unpredictable temperature fluctuations associated with climate change. However, genetic improvement of this trait is hindered by challenges in accurate phenotyping, particularly at the adult stage, and by its complex genetic architecture involving numerous minor-effect loci. While genomic selection (GS) offers a promising solution to accelerate genetic gain, its predictive ability is often limited by statistical noise from uninformative markers within whole-genome marker sets. This study aimed to overcome this limitation by developing a robust phenotypic index and implementing a genome-wide association study (GWAS)-informed GS strategy. Results We phenotyped 192 pepper accessions from a core collection for cold tolerance using a visual survival score (Surv) and a newly developed composite cold-tolerance index (CTI). Both CTI ( h 2 = 0.55) and Surv ( h 2 = 0.53) showed moderate heritability, suggesting a substantial contribution from additive genetic variance to the phenotypic variation of cold tolerance in adult plants. GWAS identified 13 candidate genomic regions associated with cold tolerance; these regions included TRM9 , CAP1 , and PP2A-2 , genes previously implicated in abiotic stress responses. A GWAS-informed GS model using a selected subset of 1,024 markers achieved a prediction accuracy of 0.78, representing a substantial improvement over that obtained with the standard model using the full marker set of 73,502 markers (0.203). Notably, a control model using a random marker set of identical size (1,024 markers) yielded an accuracy of only 0.122, confirming that the greater predictive power of the GWAS-informed GS model was driven by the genetic relevance of the selected markers rather than by lower marker density. Conclusions Our study demonstrates that assessing cold tolerance via the CTI is pivotal for overcoming the limitations of small-scale sample collection. By turning ordinal data into a continuous spectrum, the CTI can effectively unmask hidden genetic variation. Integrating this refined phenotype with GWAS-informed marker selection into prediction models significantly enhanced the accuracy of genomic prediction for cold tolerance in adult pepper plants. This integrated framework offers a practical and efficient roadmap for accelerating breeding cycles and improving selection precision for complex abiotic stress traits in pepper breeding.
Why it matches plant phenotyping methods成体ペッパーの耐寒性を測定する新規複合表現型指数(CTI)を開発し、視覚スコアとの比較および遺伝的予測への有用性を評価しており、表現型取得法が研究の中心である。
abstractThis study aimed to overcome this limitation by developing a robust phenotypic index and implementing a genome-wide association study (GWAS)-informed GS strategy.
This paper investigated the feasibility of snapshot multispectral fluorescence imaging for nondestructive identification of cold stress in pepper plants. Fluorescence spectra were obtained by exciting the plant with a 405 nm ultraviolet LED. The plants were grown under three temperature conditions: 17 °C (control), 10 °C (moderate cold stress), and 5 °C (severe cold stress). Raw fluorescence spectra extracted from the demosaiced snapshot images were used as inputs for a deep-learning pipeline consisting of feature extraction, an encoder-decoder GRU, and a multilayer perceptron (MLP), and the results were compared with conventional machine learning classifiers, including linear discriminant analysis (LDA), quadratic discriminant analysis (QDA), and a Gaussian support vector machine (G-SVM). Tukey's HSD test indicated that the proposed deep-learning model achieved the highest cross-validation accuracy and consistently produced superior classification metrics (accuracy of 85.7%, precision of 85.3%, recall of 85.3%, F1-score of 85.2). The trained model was further applied to hyperspectral cubes to generate classification maps; however, moderate misclassification was observed, consistent with the overall prediction performance.
Why it matches plant phenotyping methodsスナップショット多波長蛍光画像からピーマンの低温ストレス状態を推定する画像取得・深層学習手法が研究の中心であり、性能比較と検証も実施している。
abstractThis paper investigated the feasibility of snapshot multispectral fluorescence imaging for nondestructive identification of cold stress in pepper plants.
To address the high-throughput real-time detection requirements in industrial seed sorting scenarios, this study proposes an innovative solution coupling a lightweight detection algorithm with an industrial control system. By optimizing and integrating the YOLOv11-S architecture with the MobileNetV4 depth-wise separable convolution backbone, introducing the Focus operation for 4x downsampling via slicing concatenation without increasing computation, and embedding a mixed local channel attention mechanism, an industrially applicable model, Anomalous Seed Detection-YOLO(ASD-YOLO), with a parameter size of only 9.5 MB, was constructed. This model achieves a mean average precision (mAP) of 96.5 % while reaching a maximum processing capability of 62 FPS on a single device. Simultaneously, by incorporating algorithms such as a feedback error correction mechanism developed in conjunction with an industrial-grade pulse coordination control mechanism, the system achieves stable end-to-end latency control at the 35 ms level in a pepper seed anomaly detection production line environment. It supports continuous 24-h stable operation at a throughput of 10,000 seeds/min, with a relative error controlled to 3.3 mm. Based on the detection results, a fuzzy grading algorithm was developed to categorize the seed quality into five levels using membership functions. This provides a quantitative basis for refined storage management and differentiated processing, achieving a statistically significant 16.2 % reduction in the misjudgment rate compared with traditional grading methods. By constructing an “artificial intelligent algorithm-pulse coordination-protocol coupling” trinity architecture, the proposed model establishes a universal methodological framework for lightweight model deployment in agricultural intelligent manufacturing scenarios, offering a scalable standardized solution for seed quality control.
Why it matches plant phenotyping methods種子の異常を画像検出し品質を5段階評価する軽量モデルと産業用制御システムを開発しており、植物器官の状態取得・抽出が研究の中心である。
abstractthis study proposes an innovative solution coupling a lightweight detection algorithm with an industrial control system
Robotic pollination represents a pivotal component of smart agriculture, with foundational architectures for target recognition, path planning, and motion control having been progressively established. However, developing an efficient and robust pollination system that integrates perception, decision-making, and execution within real-world scenarios remains confronted with complex challenges. This study systematically reviews recent advancements in the field and distills the core technical issues of greenhouse robotic pollination into three primary domains: target detection and pose estimation, end-effector design, and pollination strategies combined with motion control. Focusing on the visual perception of flowers, actuator architecture, and operational tactics, this review synthesizes existing academic findings to evaluate the state-of-the-art in flower detection and pose estimation, characterize diverse end-effector designs, and analyze the evolutionary trajectory of motion control techniques. Specifically, the analysis encompasses the impact of detection algorithms on recognition accuracy and robustness, the structural classification and performance attributes of pollination mechanisms, and the optimization of control strategies. Furthermore, the study categorizes global research backgrounds, technical methodologies, and paradigmatic system cases, offering a critical evaluation of experiences in constructing automated pollination systems. Despite these advances, current robotic pollination technologies for peppers (chili) face significant bottlenecks characterized by immature methods for precise flower detection and pose estimation, the need for optimized specialized end-effector designs, and insufficient robustness in decision-making systems under dynamic environmental conditions. To address these issues, future development should prioritize constructing diverse, large-scale flower image and pose datasets while developing detection algorithms adaptable to complex environments to achieve high-precision identification. Additionally, implementing this system requires a hierarchical architecture where perception drives adaptive actuation. Deep learning models must localize flower targets and assess maturity in real-time, feeding coordinates to path planners that generate collision-free trajectories through foliage. These trajectories are executed via multimodal motion control, synchronizing the rigid manipulator with soft end-effectors. By embedding tactile feedback into the machine learning loop, the system creates a unified sensorimotor framework. This enables dynamic force modulation based on physical resistance, ensuring precise, non-destructive pollination tailored to chili plants.
Why it matches plant phenotyping methods温室コショウの花の検出・姿勢推定など、植物器官の観測・形質抽出を中核とするロボット受粉技術のレビューであり、方法論的貢献が中心。
abstractThis study systematically reviews recent advancements in the field and distills the core technical issues of greenhouse robotic pollination into three primary domains: target detection and pose estimation, end-effector design, and pollination strategies combined with motion control.
Plant Leaf Diseases represent a significant risk to global agricultural production. Crops ranging from Peppers and Tomatoes to Potatoes are affected by these diseases. Traditional methods of identifying leaf diseases based primarily on visual inspection have historically been slow and relatively inaccurate. A deep learning-based solution for the automated identification of plant leaf diseases utilizes Convolutional Neural Networks (CNNs) as the primary methodology. To identify leaf images into 15 disease categories, both pre-trained models such as VGG16, EfficientNetB3, Inception V3 and a custom CNN model were used. Using pre-trained models to allow for the use of Transfer Learning helps to mitigate some of the issues associated with computational resource limitations and data limitations in providing faster convergence rates and higher accuracy when compared to training a model from scratch. The Plant Village image collection which contains over 20,000 images of different plant leaf diseases was utilized for training and testing purposes. Each model's performance was evaluated based on its accuracy, loss and generalization capabilities. Additionally, each model was fine-tuned through hyperparameter optimization. As a result, the model that achieved the highest validation accuracy rate of 95% was the EfficientNetB3 model while the second highest accuracy rate was achieved by the Inception V3 model at 92%. This methodology provides an excellent answer to addressing early disease detection, enabling farmers to take the necessary actions quickly to reduce their losses and maximize their harvest.
Why it matches plant phenotyping methods植物葉画像から病害状態を推定する深層学習手法を比較・評価しており、病害フェノタイピングと手法検証が研究の中心です。
abstractA deep learning-based solution for the automated identification of plant leaf diseases utilizes Convolutional Neural Networks (CNNs) as the primary methodology.
Reproduction assets foundThe paper's plant-phenotyping measurements (CNN classification of 15 leaf disease classes) were performed on the public PlantVillage-derived Kaggle 'Plant Disease Dataset' by E. Marrex, which the authors cite as their training/testing image source. No author analysis code, trained model checkpoints, or paper-specific衍生Dataset · publicD. E. Popescu, M. K. Chowdary, and J. Hemanth, "Deep learning-based leaf
disease detection in crops using images for agricultural applications," Agronomy, vol. 12, no. 10,
p. 2395, 2022. doi: 10.3390/agronomy12102395. Available:
https://doi.org/10.3390/agronomy12102395.11. E. Marrex, "Plant Disease Dataset," Kaggle, Available:
https://www.kaggle.com/datasets/emmarex/ plantdisease. [Accessed: 03- Apr-2025].Open asset ↗Kagglepdf-raw-page:12 lines:1-8Plant phenotyping relevance match · UnverifiedCrossref · checked 14 Sept 2026
Published18 Feb 2026International Journal For Multidisciplinary ResearchCited by 0 · OpenAlex ↗
Plant diseases continue to pose a serious challenge to agriculture, leading to substantial yield losses and posing a threat to global food security. Accurate and early identification of plant diseases is crucial for effective crop management. However, traditional manual inspection methods are time-consuming, subjective, and heavily dependent on expert knowledge. With recent advances in artificial intelligence and computer vision, automated plant disease detection systems have emerged as a reliable alternative. These systems depend strongly on large, well-annotated image datasets for training and evaluation. Among publicly available resources, the PlantVillage dataset is one of the most widely used benchmarks for plant disease research. It contains over 50,000 high-resolution RGB leaf images captured under controlled conditions and covers 14 crop species, including tomato, potato, and bell pepper, along with multiple other plants. The dataset represents 38 distinct classes encompassing both healthy and diseased leaf categories. All images are collected against uniform backgrounds, providing visual consistency and making the dataset suitable for benchmarking machine learning and deep learning–based plant disease classification models. This survey provides a comprehensive review of the PlantVillage dataset and its contribution to the advancement of automated plant disease detection. It traces the progression from traditional handcrafted feature–based classifiers to convolutional neural networks, transfer learning approaches, and recent transformer-based architectures. Various methods are compared in terms of classification accuracy, generalization ability, computational efficiency, and robustness. In the survey we have identified that Transfer Learning model showed 99.75% accuracy. The survey also discusses key limitations of the dataset, particularly its controlled imaging conditions and challenges related to real-field deployment. Finally, future research directions are highlighted, including domain adaptation, explainable artificial intelligence, multi-disease recognition, and real-world agricultural applications. This work aims to offer researchers a structured understanding of the PlantVillage dataset and support the development of next-generation intelligent crop disease diagnostic systems.
Why it matches plant phenotyping methodsPlantVillage画像を用いた植物病害状態の画像ベース推定手法とデータセットを中心に、複数の機械学習手法を比較・レビューしているため、植物フェノタイピング方法論のレビューとして含める。
abstractThis survey provides a comprehensive review of the PlantVillage dataset and its contribution to the advancement of automated plant disease detection.
Pepper / chilliField / plotLiDAR / point cloudWhole plant / canopy / plot / fieldClassificationObject detectionSegmentationTrackingGrowth / development / phenology
Pepper (Capsicum annuum) is a globally significant horticultural crop cultivated for its culinary, medicinal, and economic value. Traditional approaches for boosting the agricultural production of pepper, notably, expanding farmland, have become increasingly unsustainable. Recent advancements in artificial intelligence and 3D computer vision have started to transform crop cultivation and phenotyping, which has shed new light on increasing production by advanced breeding. However, currently, the field still lacks 3D pepper data that contains enough detail for organ-level analysis. Therefore, we propose Pepper-4D, a new, high-precision 4D point cloud dataset that records both the spatial structure and temporal development of pepper plants across various continuous growth stages. Our dataset is divided into three subsets, including a total of 916 individual point clouds from 29 indoor-cultivated pepper plant samples. Our dataset provides manual annotations at both the plant-level and organ-level, supporting phenotyping tasks such as pepper growth status classification, organ semantic segmentation, organ instance segmentation, organ growth tracking, new organ detection, and even the generation of synthetic 3D pepper plants.
Why it matches plant phenotyping methods植物の器官レベル表現型解析を支援する4D点群データセットを構築し、成長状態分類・器官分割・追跡などを可能にする研究であり、フェノタイピング用データ基盤が中心です。
abstractPepper-4D, a new, high-precision 4D point cloud dataset that records both the spatial structure and temporal development of pepper plants across various continuous growth stages.
Reproduction assets foundThe authors publicly release the Pepper-4D spatiotemporal 3D pepper point cloud dataset (with plant- and organ-level annotations) and associated code via a GitHub repository stated in the Data Availability Statement. CloudCompare is a generic third-party tool, not a paper-specific asset.Dataset · public.J.; writing—original draft preparation, F.A.; writing—review and editing, D.L.; visualization, F.A. and D.L.; supervision, D.L.; project administration, H.Y.; funding acquisition, D.L. and H.Y. All authors have read and agreed to the published version of the manuscript.
Data Availability Statement
Data and code can be found at https://github.com/foysalahmed10/Pepper-4D (accessed on 9 February 2026).
Conflicts of Interest
The authors declare no conflicts of interest.
Funding Statement
This work was supported in part by the Shanghai Sailing Program under Grant 24YF2701200, in part by the Fundamental Research Funds for the Central Universities under Grant 2232025D-50, and in part by Donghua UnOpen asset ↗foysalahmed10/Pepper-4Dlines:238-264Code · publicang Q., Zeng Y., Hou J., Zhe X. WarpingGAN: Warping multiple uniform priors for adversarial 3D point cloud generation; Proceedings of the IEEE/CVF Conference on Computer Vision and Pattern Recognition; New Orleans, LA, USA. 18–24 June 2022; pp. 6397–6405.
Associated Data
Data Availability Statement
Data and code can be found at https://github.com/foysalahmed10/Pepper-4D (accessed on 9 February 2026).Open asset ↗foysalahmed10/Pepper-4Dlines:308-314Plant phenotyping relevance match · UnverifiedEurope PMC · checked 5 Sept 2026
Food security continues to be a significant challenge the world over, with crop production becoming increasingly threatened by crop diseases and pest infestations. In the case of chili production, farmers often suffer significant yield loss and economic insecurity due to the unpredictable nature of both of these problems. Current pest control options (agrochemical and organic methods alike) have not reliably been enough for timely and effective control and demonstrate the importance of early and effective pest and disease identification processes. To solve this problem, the present work describes a deep learning implementation that leverages advanced YOLO-based architectures for the detection and classification of leaf diseases and pest on chili plant leaves. The present work trains and evaluates three models (YOLOv5, YOLOv7, and YOLOv8) on a newly created and balanced dataset of over 28,800 images combining 20 total classes of pest and leaf diseases. The dataset was supplemented by preprocessing the images and conducting an augmentation process to create a total of 32,000 images for training to generate reliable models. The results from the experiments found that YOLOv8 provided the best baseline performance of 95.1% mean Average Precision (mAP), while YOLOv5 had an mAP of 86.1%, and YOLOv7 had an mAP of 67.5%. An additional enhancement in the construction of a Modified YOLOv8 hybrid model—reflecting all advantages of YOLOv5, YOLOv7, and YOLOv8—achieved a highest mAP of 99.5% to be the most effective model in this study, the results suggest that the newly proposed Modified YOLOv8 framework, is highly accurate and reliable for the early detection of pests and diseases in chili, and is helpful to improve sustainable agricultural practices, mitigate crop losses, and increase global food security.
Why it matches plant phenotyping methodsチリ葉の病害を画像から検出・分類する深層学習手法の開発、比較評価、データセット構築が研究の中心であり、植物の病害状態を直接推定するため対象範囲に該当する。
abstractthe present work describes a deep learning implementation that leverages advanced YOLO-based architectures for the detection and classification of leaf diseases and pest on chili plant leaves.
Chili pepper (Capsicum annuum L.) is a strategic horticultural commodity in Indonesia with high economic value. However, chili plants are often infected by diseases such as Anthracnose, Fusarium Wilt, Fruit Fly, and Thrips, which can lead to significant yield losses. Early and accurate identification of these diseases is crucial for effective control measures. This study aims to classify chili plant diseases based on leaf images using the Gray Level Co-occurrence Matrix (GLCM) for feature extraction and the K-Nearest Neighbor (K-NN) algorithm for classification. A total of 736 leaf images were used, divided into four disease classes. The pre-processing stages included resizing the images to 300×300 pixels, rotation augmentation (0°, 45°, 75°, 90°), and conversion to grayscale. Textural features were extracted using GLCM at four angles, and K-NN was applied with K values of 5, 7, and 9. The highest classification accuracy of 88.19% was achieved at a GLCM angle of 0° and K=5, with an overall average accuracy across all angles of 85.06%. These findings not only reinforce previous findings on the effectiveness of GLCM and K-NN but also contribute by identifying the optimal parameter configuration (angle 0° and K=5) for the specific chili disease dataset. The results have the potential to be applied as a foundation for developing an automated plant disease detection system in the field.
Why it matches plant phenotyping methods葉画像から植物病害状態を推定する画像特徴抽出・分類手法が研究の中心であり、精度評価とパラメータ比較も行っているため、植物フェノタイピング手法として採用。
abstractThis study aims to classify chili plant diseases based on leaf images using the Gray Level Co-occurrence Matrix (GLCM) for feature extraction and the K-Nearest Neighbor (K-NN) algorithm for classification.
Pepper / chilliField / plotFruitClassificationObject detectionGrowth / development / phenology
Accurate identification of pepper berry maturity is essential for ensuring optimal harvest timing and maintaining quality standards in spice production. This study proposes " YOLO-AVCA-CBAMNet" an integrated detection-and-classification framework designed to operate effectively under natural field conditions. A self-collected dataset of pepper berries, captured using a smartphone across diverse illumination settings and background complexities, forms the basis of the evaluation. The pipeline first applies YOLOv8 to detect individual berries within cluttered scenes. The extracted regions are then classified using convolutional neural networks enhanced with two complementary attention mechanisms. The Adaptive Visual Cortex Attention Module (AVCAM) strengthens global contextual weighting by adaptively recalibrating salient features, while the Convolutional Block Attention Module (CBAM) improves spatial and channel-specific discrimination through sequential attention refinement. This dual-attention design enables more reliable separation of visually similar maturity stages. Experimental results indicate accuracy gains of 5-9 % across all backbone architectures, with the DenseNet121-based configuration achieving a peak accuracy of 96.19 % . The findings demonstrate the potential of attention-driven models to support interpretable, efficient, and scalable maturity assessment solutions in precision agriculture.•Developed an end-to-end framework "YOLO-AVCA-CBAMNet" integrating object detection and attention-driven classification for pepper maturity assessment in natural field conditions.•Employed a field-derived image dataset of pepper berries collected under naturally varying illumination and environmental conditions, thereby supporting the ecological validity and practical relevance of the proposed maturity assessment approach.•Incorporated complementary attention mechanisms-AVCAM to enhance global contextual representation and CBAM to refine spatial and channel-specific feature responses-thereby improving discrimination among visually similar maturity stages.
Why it matches plant phenotyping methods圃場画像から個々のトウガラシ果実を検出し、成熟段階という植物器官の状態を分類する手法を開発・評価しており、フェノタイピング手法が中心である。
abstractThis study proposes " YOLO-AVCA-CBAMNet" an integrated detection-and-classification framework designed to operate effectively under natural field conditions.
With the continuous improvement of the intelligence level of facility agriculture, agricultural robots are undertaking more and more autonomous tasks in greenhouse environments, and the multifunctional integration of visual perception systems has become a key technological bottleneck. A perception system for agricultural robots that integrates visual navigation and phenotype recognition is developed to address issues such as path recognition being susceptible to environmental interference and poor real-time plant detection. The system consists of a path navigation module and a plant detection module. The former introduces an image segmentation method based on visual transformation structure to extract agricultural path information. The latter adopts a lightweight instance segmentation structure to achieve precise segmentation and structural localization of crop phenotype regions. In the navigation model test, in the rain and fog disturbance scene, the average delay is 51.0 ms, the frame rate is 44.2 FPS, and the control jitter amplitude is 1.36°. The test results of the detection module show that its boundary F1 values for Tomato, Cucumber, Pepper, and Lettuce crops are 90.2%, 87.6%, 88.8%, and 86.3%, respectively. The experimental results show that the proposed scheme reduces inference delay while ensuring accuracy, has good environmental adaptability and edge deployment potential, and demonstrates good robustness and practicality in complex greenhouse environments.
Why it matches plant phenotyping methods植物の表現型領域を画像分割・認識する手法を開発し、複数作物で精度と実時間性能を評価しており、表現型取得が中心的な技術貢献です。
abstractA perception system for agricultural robots that integrates visual navigation and phenotype recognition is developed
Addressing the challenges of variable target morphology, small critical regions, and complex background interference in eggplant picking point detection within complex agricultural scenarios, this study proposes MDAD-YOLO (Multi-dimensional Attention and DySample YOLO), a detection model improved based on the YOLOv10n-pose framework. First, the model’s cross-dimensional perception ability for fruits and picking points is enhanced by integrating the collaborative mechanism of regional receptive field attention with channel-space joint attention. Next, within the Neck structure, coordinate attention is incorporated to optimize the spatial localization accuracy of fine-grained features, enhancing sensitivity to minute regions such as the fruit stem apex. Additionally, dynamic pixel reorganization is applied to enhance feature map reconstruction details, addressing the detail loss caused by traditional interpolation methods. Finally, cascading adaptive fine-grained channel attention with position-sensitive attention enables multi-level modeling of channel dependencies and collaborative spatial context enhancement. Through a seven-tier validation framework, the model’s effectiveness, robustness, and generalizability have been comprehensively demonstrated. Experimental results show that the model achieves 93.6% mAP@50 for object detection, 94.7% mAP@50 and 92.1% mAP for keypoints detection, and an average pixel Euclidean distance error of 19.41 on the self-built eggplant dataset, outperforming YOLOv12 and other high-performance models. Additionally, cross-crop experiments on the pepper dataset showed a 2.1% and 2.7% improvement in mAP for object and picking point detection, respectively, compared to the baseline model, confirming its cross-crop robustness. This study reveals the synergistic enhancement of dynamic upsampling and attention mechanisms in agricultural object detection, providing new insights for lightweight model design in complex scenarios.
Why it matches plant phenotyping methods果実と収穫点の画像ベース検出・キーポイント推定モデルを開発し、複数データセットで性能と頑健性を検証しているため、植物形質取得手法が中心である。
abstractthis study proposes MDAD-YOLO (Multi-dimensional Attention and DySample YOLO), a detection model improved based on the YOLOv10n-pose framework.
Abstract: Agriculture forms a cornerstone of the Indian economy, with food and cash crops playing a critical role in sustaining both the environment and human livelihoods. However, crop yields are significantly impacted each year by various plant diseases. The lack of efficient diagnostic methods, combined with limited awareness of disease symptoms and treatment options, often leads to widespread crop losses. This study explores the application of machine learning for plant disease detection, focusing on Convolutional Neural Networks (CNNs) to identify and classify diseases. The proposed approach employs advanced image processing techniques to analyze infected leaf regions, examining metrics such as time complexity and lesion area. The model was trained and tested on a curated dataset comprising 15 cases, including 12 disease categories such as Bell Pepper Bacterial Spot, Potato Early Blight, and Tomato Leaf Mold, alongside 3 categories of healthy leaves. The system achieved a test accuracy of 88.8%, demonstrating its potential for accurate plant disease detection. Performance evaluation was conducted using standard metrics to validate the model's reliability.
Why it matches plant phenotyping methods感染葉の画像から病害を分類し、病斑面積を解析するCNN手法の開発・評価が中心であり、植物の病害状態を直接推定するため含める。
abstractThis study explores the application of machine learning for plant disease detection, focusing on Convolutional Neural Networks (CNNs) to identify and classify diseases.
High-precision plant phenotyping requires efficient 3D reconstruction with high fidelity, yet existing methods such as MVS and NeRF all have problems of feature dependence and error accumulation during 3D reconstruction, which leads to geometric distortion in reconstruction and restricts the reconstruction efficiency. To address this bottleneck, this study first determined the multi-view image acquisition strategy. Further, based on the self-built multi-view dataset of chili peppers, it proposed an algorithm for efficient and high-fidelity 3D reconstruction of complex plant structures through global adaptive pose optimization and gaussian splash rendering technology, referred to as the GAPose-GS algorithm. Experimental results indicate that the Peak Signal-to-Noise Ratio ( PSNR ) improves by 52.0 %, 26.4 %, and 4.2 % compared to NeRF, Instant-NGP, and 3D Gaussian Splatting respectively. Additionally, the Structural Similarity Index Measure ( SSIM ) increases by 22.9 %, 12.8 %, and 4.3 % respectively over above methods. The point cloud data reconstructed based on this algorithm also has advantages in the measurement of phenotypic parameters. Compared with the actual measured values, the R² of the phenotypic parameters such as pepper plant height, canopy width, and leafstalk angle obtained in this study are 0.997, 0.954 and 0.978 respectively, and the RMSE are 0.236 cm, 1.082 cm and 2.344° respectively, and the MAE are 0.209 cm, 0.880 cm and 1.965° respectively. The accuracy was significantly better than that of the existing phenotypic calculation methods. Verification across different growth stages of wheat and maize was performed universally, with all errors remaining below 1.1 %, providing new ideas and technologies for high-precision, low-cost, and high-throughput crop phenotypic research.
Why it matches plant phenotyping methods植物の多視点画像から3D再構成し、草丈・群落幅・葉柄角などの形質を推定する手法を開発・比較検証しており、フェノタイピング手法が中心的である。
abstractit proposed an algorithm for efficient and high-fidelity 3D reconstruction of complex plant structures through global adaptive pose optimization and gaussian splash rendering technology, referred to as the GAPose-GS algorithm.
Introduction. Chlorophyll plays a crucial role in absorbing and transforming light energy into a chemical form that provides organic matter production in plants. Monitoring of chlorophyll content helps to assess plant-environment interactions and the degree of influence of stress factors that are essential for yield management. Traditional laboratory methods of analyzing are time-consuming, destroying samples and unsuitable for rapid field evaluations. A more reasonable solution is to use lowcost, portable devices. Aim of the Study. The study is aimed at developing and training an ANN architecture to predict the chlorophyll content in plant leaves based on their optical density within specific visible spectrum ranges. Materials and Methods. The artificial neural network dataset was compiled from experi- mental measurements using the DP-1M densitometer and the CCM-200 chlorophyll meter. Data were collected from lettuce, pepper, tomato and zucchini leaves of different ages, which were grown in different light environments. The artificial neural network training was carried out in the Google Colab environment with subsequent adaptation of the model for using in a microcontroller device – a photocolorimeter for leaves. Results. The dataset with 1,000 entries showed that the leaf optical density range isfrom 0.57 to 2.54 relative units (red), from 0.9 to 1.66 relative units (green), and from 1.09 to 3.53 relative units (blue). According to these data, the chlorophyll content variations are from 3.1 to 156.5 relative units. In the study, there were compared six artificial neural network architectures that differed by hidden-layer neurons. The structure “32:32” had the highest accuracy (MAE = 6.64 rel. units, MAPE = 16.34%, R² = 0.8886). A simplified structure “4:4” was selected to simplify the model and improve the microcontroller efficiency. This structure maintained the performance (MAE = 6.83 rel. units, MAPE = 16.86%, R² = 0.8808) with much smaller amount of resources used – 41 weight parameters and 164 bytes of memory. A comparative evaluation with classical machine learning algorithms demonstrated the superiority of the developed model across all metrics. Discussion and Conclusion. The trained artificial neural network was implemented on a microcontroller-based photocolorimeter for leaves that enabled the non-destroying optical density measurements. The developed model allows implementing non-destroying and operational monitoring of the condition of plants, which is especially important in precision farming systems. This approach has significant potential for ecological monitoring and precision agriculture. The study results demonstrate the viability of machine learning for improving plant status assessment and developing digital agrotechnology solutions.
Why it matches plant phenotyping methods葉の光学密度からクロロフィル含量を推定するANNとマイコン実装型フォトカラリメータを開発・比較評価しており、植物形質取得が研究の中心です。
abstractThe study is aimed at developing and training an ANN architecture to predict the chlorophyll content in plant leaves based on their optical density within specific visible spectrum ranges.
Plant growth chambers provide controlled environments for agricultural research, enabling precise monitoring of crop diseases under optimal microclimate conditions. This paper presents an integrated IoT-based smart plant growth chamber system utilizing YOLOv8 machine learning for the automated classification of anthracnose disease severity in chili peppers (Capsicum annuum L.). The system integrates multiple subsystems, including environmental control, a robotic camera with 2-axis movement, a gateway for data communication, and remote monitoring capabilities through a cloud server and a web interface. Dataset labeling was performed using LabelImg and Roboflow, with data augmentation increasing training samples from 70% to 86%. Three YOLOv8 models were evaluated: YOLOv8L (150 epochs), YOLOv8N (100 epochs), and YOLOv8N (398 epochs). Based on our test so far, the YOLOv8L model achieved the best performance with mAP of 67.4% and successfully detected 44 out of 102 test samples (43% detection rate) across multiple disease severity scores (0-9). The system enables both onsite and remote access, automatic data logging, real-time image capture with PyQt5-based GUI, and environmental parameter control (temperature: 5-50°C, humidity: 40-90%RH, light: 0-15,000 lux), which can be manually set and automatically set based on the requirements of the user. This integrated approach demonstrates practical deployment of edge AI and IoT technologies for precision agriculture and disease monitoring applications.
Why it matches plant phenotyping methods植物の病害症状を画像から重症度分類するYOLOv8ベースの撮像・解析システムが研究の中心であり、植物状態の表現型推定手法として適格。
abstractThis paper presents an integrated IoT-based smart plant growth chamber system utilizing YOLOv8 machine learning for the automated classification of anthracnose disease severity in chili peppers (Capsicum annuum L.).
While the root architecture of potted crop seedlings directly determines subsequent crop productivity and adaptability, these root systems remain challenging to quantify using conventional methods due to their structural complexity. To investigate the microscopic characteristics of the root systems of pepper seedlings within pots, Micro-CT was employed to scan the seedling pots. After three-dimensional (3D) reconstruction was conducted on the data acquired from the pot scans, the 3D model of the root system was segmented and extracted using the watershed algorithm. Vertically, the three-dimensional root model was divided from top to bottom into four equally spaced regions (a, b, c, and d), showing the volumetric distribution characteristics of pepper seedling roots within the pots. The results showed that region a had the largest average root volume proportion (29.72%), primarily due to the substantial volume contribution of the taproot. Region d followed with an average proportion of 27.26%, resulting from root coiling and entanglement at the pot bottom caused by the spatial constraints of the seedling tray. The middle regions of the pot, b and c, showed average root volume proportions of 23.14% and 19.89%, respectively. To further investigate the influence of root system characteristics on root injury during seedling gripping, the seedlings were categorized into three types based on their taproot growth positions. A gripping experiment was conducted on these three seedling types using spatula-equipped needles. The results showed that the greatest root injury (12.67%) was observed in Type 1 seedlings, which had taproots located closest to the needle insertion point. In contrast, the least injury (4.09%) was found in Type 3 seedlings, characterized by centrally positioned taproots. Type 2 seedlings, with their taproots growing on the side (laterally away from the insertion point), sustained intermediate injury (5.45%). This was because their lateral positioning led to an uneven distribution of mechanical stress during gripping compared with Type 3 seedlings. A validation experiment conducted on an automated seedling retrieval platform confirmed the root injury analysis. The experimental results showed maximum root injury in Type 1 seedlings (14.16%), followed by Type 2 (6.03%) and Type 3 (4.82%) seedlings, with a successful retrieval rate of 95.29%. These findings were consistent with the Micro-CT analysis. This study could provide a theoretical foundation for low-injury seedling gripping in fully automated seedling transplanters.
Why it matches plant phenotyping methodsMicro-CT、3D再構成、watershed分割を用いて苗の根系形態を定量化する手法が研究の中心であり、根容積分布と根傷害の評価まで検証している。
abstractMicro-CT was employed to scan the seedling pots. After three-dimensional (3D) reconstruction was conducted on the data acquired from the pot scans, the 3D model of the root system was segmented and extracted using the watershed algorithm.
Chili plants (Capsicum annuum L.) constitute a significant horticultural commodity in Indonesia, with production on the rise. Chili producers frequently encounter difficulties, including insufficient knowledge regarding diseases that impact chili plant foliage and the application of technology. Identified major diseases include leaf spot, curly top, and gemini, which can diminish chili output. The application of the ResNet 101 architecture in Convolutional Neural Networks (CNN) for disease detection in plant pictures presents a viable approach. The ResNet 101 architecture is employed to recognize and classify various illnesses on chili leaves, facilitating early symptom detection and diagnosis. The residual architecture (ResNet 101) acquires intricate elements in images to improve disease diagnosis precision. This research examines the utilization, challenges, and benefits of disease detection systems through various methodologies, including observations and field investigations. The process entails gathering picture models from various disease categories (leaf spot, curly top, gemini) and healthy leaves to construct a dataset, in addition to seeking expert consultation on illness classifications and employing the ResNet 101 architecture for modeling. The use of the ResNet 101 architecture in the CNN model for disease detection on chili leaves, utilizing a dataset of 1,518 images categorized into four groups (leaf spot, curly top, gemini, and healthy leaves), yielded substantial outcomes, achieving an overall accuracy of 0.9382. The established architecture must be evaluated against alternative designs to enhance the model's outcomes and to expand the dataset utilized to improve accuracy substantially.
Why it matches plant phenotyping methodsチリ葉の画像から病害状態を分類するCNN手法の開発・評価が中心であり、植物病害の表現型推定に該当する。
abstractThe application of the ResNet 101 architecture in Convolutional Neural Networks (CNN) for disease detection in plant pictures presents a viable approach.
We explored the application of hyperspectral imaging (400-1100 nm) for non-destructive evaluation of peroxidase (POD) and polyphenol oxidase (PPO) enzymes responsible for browning processes in bell pepper (Capsicum annuum L.) cultivars. Several preprocessing techniques, including Standard Normal Variate (SNV), were applied to spectral data to enhance signal quality. Analysis using Partial Least Squares Regression (PLSR) showed that raw spectral data provided stronger correlations and lower prediction errors compared to processed. Discriminant spectral bands were identified using Support Vector Machine (SVM) combined with metaheuristic optimization, with SVM-Learning Automata (LA) resulting as the most effective wavelength selection strategy. Enzyme activities were then predicted using selected wavelengths with Artificial Neural Network (ANN) and PLSR models. Model performance was evaluated using the coefficient of determination (R 2 ), Root Mean Square Error (RMSE), and Ratio of Performance to Deviation (RPD) on independent validation sets. ANN consistently outperformed PLSR, achieving high cultivar-specific R 2 values for POD of 0.86, 0.93, and 0.98, for Orange, Yellow, and Red pepper varieties, respectively and PPO R 2 values of 0.91, 0.97, and 0.99, for the same pepper cultivars. A combined "Total Model" integrating data from all cultivars further demonstrated robust generalization, with R 2 values of 0.9082 for POD and 0.9604 for PPO. Findings confirm that hyperspectral imaging, coupled with an effective wavelength selection technique and ANN modeling provides a rapid, reliable, and robust approach for industrial evaluation of enzymatic activity in bell peppers. The proposed methodology offers significant potential for quality monitoring, process optimization, and large-scale application in industrial environments.
Why it matches plant phenotyping methodsピーマンの酵素活性という植物状態を、ハイパースペクトル画像と波長選択・機械学習で非破壊推定する方法が研究の中心で、独立検証も実施している。
abstractWe explored the application of hyperspectral imaging (400-1100 nm) for non-destructive evaluation of peroxidase (POD) and polyphenol oxidase (PPO) enzymes responsible for browning processes in bell pepper (Capsicum annuum L.) cultivars.
Abstract Inefficient fertilizer application in agriculture leads to reduced crop productivity, nutrient losses, and reduced crop resilience, highlighting the urgent need for real-time monitoring of plant–soil nutrient dynamics. This research aims to develop and validate a multiplexed sensing platform for simultaneous, in-situ measurement of key soil nutrients (Soil-FIT) and plant phytohormones (Stem-FIT) involved in nitrogen signaling and stress regulation. The proposed sensor suite integrates three 3D-printed modules for continuous monitoring of nitrate, ammonium, and pH in both soil and plant sap, along with salicylic acid (SA), indole-3-acetic acid (IAA), methyl jasmonate (MeJA), and ethylene (ET) in plant sap. The sensors, functionalized with non-enzymatic electrode coatings, were deployed in bell pepper plants grown under four treatment combinations of irrigation (full vs. deficit) and nitrogen application (medium vs. high). Data were collected every three hours over the growing period and analyzed using a long short-term memory (LSTM) model for short-term prediction of nutrient and hormone fluctuations. The sensors exhibited high sensitivity and stability, achieving detection limits of 0.218 µM for IAA, 1.07 µM for MeJA, 1.315 µM for SA, 1.08 ppm for nitrate, 1.017 ppm for ammonium, 0.29 ppm for ethylene, and 0.01 pH. The LSTM model demonstrated strong predictive capability (R² = up to 0.86), accurately forecasting short-term variations in plant and soil nutrient–hormone profiles. These findings demonstrate that coupling real-time, multiplexed sensing with machine learning enables early detection and prediction of crop stress, supporting precision nitrogen management and advancing sustainable agricultural practices.
Why it matches plant phenotyping methods植物体内のホルモン・栄養状態を連続測定するセンサープラットフォームの開発と性能検証が中心で、機械学習による予測も含むため、植物フェノタイピング手法として適格です。
abstractThis research aims to develop and validate a multiplexed sensing platform for simultaneous, in-situ measurement of key soil nutrients (Soil-FIT) and plant phytohormones (Stem-FIT) involved in nitrogen signaling and stress regulation.
Plant leaf diseases compromise yield, quality, and farmer income, while late or inaccurate diagnosis drives excess pesticide use and production losses. Automated, image-based detection offers a scalable alternative to manual scouting, yet field images suffer from illumination shifts, background clutter, and class imbalance, which degrade model reliability. This work proposes an end-to-end pipeline for robust plant leaf disease recognition that combines physics-guided preprocessing with hybrid deep representations and class-aware learning. First, a preprocessing module applies illumination normalization (Retinex-inspired color constancy) and haze suppression, followed by Multi-Stage Attention Leaf Segmentation (MSALS) to isolate lamina and lesions from complex backgrounds. Next, for feature extraction, we introduce a Hybrid Shifted Vision Transformer (HS- ViT) that fuses a lightweight CNN stem (local texture cues) with cross-scale window-shifted transformer blocks (global lesion geometry), augmented by channel-spatial attention to emphasize symptomatic regions. Finally, classification uses a prototype-aware focal objective with temperature scaling to handle imbalance and sharpen decision boundaries; an uncertainty-weighted ensemble stabilizes predictions under domain shift. To improve minority classes, an Adaptive Augmentation module synthesizes realistic variations in lesion color, size, and spread. We evaluate on PlantVillage (~54k images, 38 classes) and a field collection from Telangana (chilli and sugarcane leaves; expert-annotated). The proposed system attains 99.0% top-1 accuracy on PlantVillage and 95.3% balanced accuracy in field conditions, exceeding recent CNN/ViT baselines by 2–6 percentage points and improving macro- F1 on rare diseases. The results indicate a practical path to deployable, edge-friendly diagnosis that can reduce chemical inputs and support precision agronomy at scale.
Why it matches plant phenotyping methods植物葉の病徴・病斑を画像から分離し、病害状態を推定する画像ベースの表現型解析パイプラインを開発・評価しており、フェノタイピング手法が研究の中心である。
abstractAutomated, image-based detection offers a scalable alternative to manual scouting
Abstract Particularly in the most lowland areas of Ethiopia, Pepercorn is an essential crop that makes a substantial contribution to the country's agricultural economy. However, several diseases that affect crop output and quality provide a barrier to Pepercorn production. Conventional disease detection techniques depend on specialist knowledge and manual inspections, which are frequently time-consuming and ineffective, limiting prompt response. Digital image processing, computer vision, and deep learning technologies have a lot of potential, but their use in Ethiopia's agriculture industry is still unexplored. The need for more sophisticated methods is highlighted by the fact that previous studies primarily used manual feature extraction techniques for disease detection. After a careful analysis of relevant literature, four deep learning architectures were selected: VGG16, VGG19, DenseNet121 and YOLOv11n. Several train-test data splits, such as 70%/30%, 80%/20% and 90%/10% were explored to assess model performance; the VGG19 with 90%/10% split produced the best accuracy 98.05% in case of VGGNet. And the DenseNet121 with 80%/20% achieves better accuracy 98.75% than VGG19. But YOLOv11n is the better model among the entire models researchers used. It achieves a mean average precision (MAP) of 99.03%. When we see the results in terms of performance (speed) the YOLOv11n model performs its preprocessing and post processing tasks in 1Hr, 096 Seconds, while DenseNet121 takes a speed of 2Hr, 35 Seconds. According to the study's finding, out of all the algorithms studied, YOLOv11 is the best model for Pepercorn leaf disease detection and classification.
Why it matches plant phenotyping methodsペッパーコーン葉の病害状態を画像から検出・分類する深層学習モデルを開発・比較し、精度を評価しており、植物フェノタイピング手法が研究の中心である。
titlePepercorn Leaf Disease Detection and Classification model Using Deep Learning Approach
The expected population rise will require a maximum exploitation of agricultural lands with a consequent increase in the demand for freshwater for irrigation uses. Future trends predict increasing periods of drought stress, which may impact on crop performance and limit the future production. Pepper is one of the most economically important crops and globally consumed vegetables. This crop is highly demanding in terms of water supply, and so far, developing tolerant cultivars is one of the main targets for breeding. The aim of this study is to accurately determine how pepper plants react to water stress at the vegetative stage in order to select genotypes that better cope with drought. We implemented the PhenoHort Plant Eye phenotyping platform to precisely assess changes in plant architecture and morpho-physiological parameters on 25 cultivated pepper genotypes (Capsicum annuum) under drought stress conditions. Three different irrigation supply levels were considered, including the control, intense, and severe water stress, by irrigating every 24, 72, and 96 h, respectively. Daily monitoring of 20 traits allowed ~190,000 multispectral and tridimensional data points through scans over 6 weeks of cultivation, thus shedding light on changes in plant architecture and vegetation indices’ values during stress. The dissection of genotype (G) and treatment (T) interactions revealed that digital biomass and plant height traits were strongly affected by the T factor (more than 50% of total variance), whereas color and multispectral parameters were under greater genotypic control, accounting for 58.27% and 64.97% of the total variance for HUE and NPCI, respectively. The comparison of each accession with respect to the control and the application of multivariate models allowed us to select four drought-tolerant lines (G1, G2, G22, and G25) able to reduce the effects of drought on the morphological parameters and architecture of the plant with positive effects on vegetative indices. This work represents the first attempt to dissect the response of pepper under drought stress at the vegetative stage using a high-throughput and non-invasive phenotyping system, offering new insights for selecting resilient genotypes.
Why it matches plant phenotyping methods植物の乾燥応答を評価するため、マルチスペクトル・3D高スループット表現型解析プラットフォームを中心的に実装し、植物形態・生理形質を長期的かつ非侵襲的に取得しているため。
abstractWe implemented the PhenoHort Plant Eye phenotyping platform to precisely assess changes in plant architecture and morpho-physiological parameters on 25 cultivated pepper genotypes (Capsicum annuum) under drought stress conditions.
The detection of virus infection attacking plants mainly depends on polymerase chain reaction (PCR) testing. Nevertheless, the COVID-19 pandemic, during which the availability of the PCR test was limited, highlights the need for reliable alternative methods for detecting viruses. An effective technique for diagnosing plant disease involves the use of an electronic nose (e-nose) that can detect volatile organic compounds (VOCs) emitted by plants. However, the extensive use of e-noses is limited by the noise that can come from temperature and humidity changes. In order to address this limitation, this research focused on optimising filtering techniques to improve e-nose performance in detecting pepper yellow leaf curl virus (PYLCV) infected chilli plants. The samples were taken from commercial plantations, ensuring that those infected grew in a controlled environment, and ensuring PYLCV detection in diverse conditions. The methods of Fast Fourier Transform (FFT), Discrete Wavelet Transform (DWT), and Savitzky-Golay (SG) filtering were used for the purpose of noise filtering. The optimisation of each filtering technique was performed, such as cutoff frequency for the FFT, the decomposition levels and types of mother wavelets for the DWT, and the polynomial degree and number of windows for the SG filter. The optimisation was performed using a deep neural network (DNN). As a result, the DWT symlet4 level 10 with a specific filter length outperformed the FFT and SG method, with DNN accuracy reaching 97.8% and increasing the accuracy of the unfiltered signal by 5.4%. The result was then validated with other classification models. This proves that with a suitable filtering technique, the e-nose can be a reliable instrument for plant disease detection.
Why it matches plant phenotyping methods植物が放出するVOCを電子鼻で測定し、植物ウイルス感染状態を推定する信号処理・分類手法の最適化と検証が研究の中心であるため、植物病害フェノタイピング手法として収載する。
abstractthis research focused on optimising filtering techniques to improve e-nose performance in detecting pepper yellow leaf curl virus (PYLCV) infected chilli plants.
To enhance crop yield, detecting leaf diseases has become a crucial research focus. Deep learning and computer vision excel in digital image processing. Various techniques grounded in deep learning have been utilized for detecting plant leaf diseases; however, achieving high accuracy remains a challenge. Basic convolutional neural networks (CNNs) in deep learning struggle with issues such as the abnormal orientation of images, rotation, and others, resulting in subpar performance. CNNs also need extensive data covering a wide range of variations to deliver strong performance. CapsNet is an innovative deep-learning architecture designed to address the limitations of CNNs. It performs well without needing a vast amount of data in various variations. CapsNets have their limitations, such as the encoder network considering every element in the image and the crowding issue. Due to this, they perform well on simple image recognition tasks but struggle with more complex images. To address these challenges, we introduced a new CapsNet model known as CCFM-CapsNet. This model incorporates CLAHE to reduce image noise and CDH to extract crucial features. Also, max-pooling and dropout layers are incorporated in the original CapsNet model for identifying and classifying diseases in apples, bananas, grapes, corn, mangoes, pepper, potatoes, rice, tomato and also for classifying fashion-MNIST and CIFAR-10 datasets. The proposed CCFM-CapsNet demonstrates significantly high validation accuracies, achieving 99.53%, 95.24%, 99.75%, 97.40%, 99.13%, 100%, 99.77%, 100%, 98.54%, 93.48%, and 82.34% with corresponding parameters in millions(M) 4.68M, 4.68M, 4.68M, 4.68M, 4.79M, 4.63M, 4.66M, 4.68M, 4.84M, 2.39M, and 4.84M for the datasets aforementioned respectively, outperforming the traditional CapsNet and other advanced CapsNet models. Consequently, the CCFM-CapsNet model can be utilized effectively as a smart tool for identifying plant diseases and also in achieving Sustainable Development Goal 2 (Zero Hunger), which aims to end global hunger by the year 2030.
Why it matches plant phenotyping methods植物葉画像から病害状態を推定する深層学習モデルを提案・評価しており、画像取得・分類手法が研究の中心であるため。
abstractTo address these challenges, we introduced a new CapsNet model known as CCFM-CapsNet.
Background Field-scale assessment of chili leaf curl complex presents a significant diagnostic challenge, as both chili leaf curl virus (ChiLCV) and mite infestations produce visually overlapping symptoms difficult to distinguish by untrained personnel. This diagnostic confusion frequently leads to inappropriate application of either insecticides or acaricides, resulting in economic losses and environmental concerns. To address this issue, we propose SCA-MobiPlant, an improved MobileNetV3-Small model integrated with a novel multistage Squeeze-and-Excitation Coordinate Attention (SCA) fusion mechanism, designed for accurate differentiation of these apparently similar symptoms and precise field assessment of the disease. Results The proposed model effectively focuses on subtle diagnostic features including leaf texture, petiole elongation, and irregular curling patterns to achieve reliable classification. The multistage SCA fusion module demonstrated superior performance, achieving 99.64% accuracy, 99.61% precision, 99.64% recall, and 99.62% F1-score through K = 5 cross-validation, outperforming other attention modules such as the Convolutional Block Attention Module (CBAM) and Coordinate Attention (CA). Gradient-Weighted Class Activation Mapping (Grad-CAM) provided visual interpretability of the model's decision-making process. Comparative evaluation against state-of-the-art architectures, including EfficientNetB0, ResNet50, VGG19 and YOLO advanced series, confirmed the computational efficiency of the proposed model for mobile deployment. Conclusions The final system, termed SCA-MobiPlant, has been successfully implemented on smartphones, along with a Disease Incidence (DI) calculation module, enabling rapid and accurate field assessment of the disease. This facilitates appropriate intervention strategies while minimizing unnecessary pesticide use. The study highlights the potential of lightweight, attention-enhanced models for real-world plant disease diagnostics, particularly in resource-constrained agricultural settings.
Why it matches plant phenotyping methods植物病徴画像から葉の症状を分類し、病害発生率を算出するスマートフォン実装手法の開発・比較評価が研究の中心であり、植物の病害状態を直接推定しているため。
abstractwe propose SCA-MobiPlant, an improved MobileNetV3-Small model integrated with a novel multistage Squeeze-and-Excitation Coordinate Attention (SCA) fusion mechanism, designed for accurate differentiation of these apparently similar symptoms and precise field assessment of the disease.
Abstract Background Drought stress can significantly impede plant productivity, adversely impacting crop yields. The root system is an important plant organ contributing to drought resistance mechanisms. Therefore, assessing root systems under drought stress conditions can provide insights to identify root traits associated with enhanced drought resistance. When seeking dense and high-quality root data, root phenotyping can be complex, costly, and time-consuming. The objectives of this study were to establish a method to grow chili pepper plants in a soil-based rhizobox container under water deficit conditions and compare two methods for collecting two-dimensional root trait data from their roots. Method We grew two chile peppers ( Capsicum annuum ) accessions in soil-based rhizobox containers to analyze the responses of root architecture traits under well-watered and water-deficit conditions during the vegetative stage. The root traits were phenotyped using two different methods. The first method involved non-destructive in-box imaging of roots in situ through acrylic glass while the plant grew. The second method involved scanning destructively harvested and washed roots—the gold standard for root measurements. For the first method, we developed a pipeline for rhizobox studies to demonstrate the response of root system architecture to water deficit over time and assessed the quality of non-destructive in-box imaging methods as compared to scans of destructively harvested and washed roots. We used a relatively large rhizobox (53.34 cm in width x 78.73 cm in height) into which we established and maintained well-watered and water deficit conditions based on the field capacity and permanent wilting point of the soil (Bodner et al. 2017; Cassel & Nielsen 1986).Our in-box root imaging pipeline captures high-resolution root images with an affordable camera that can achieve a maximum resolution of 9152 x 6944 pixels, as well as high-quality root segmentation using a robust graphical user interface-based software called RootPainter (Smith et al. 2022). Results Root growth decreased under water deficit compared to well-watered conditions. There were strong positive relationships between total root length using the washed scanned method and the in-box imaging method. The same was observed for root perimeter and most of the total root length distinct root diameter classes, but not for average root diameter. Some of these relationships weakened under water deficit conditions. In addition, we also found a strong relationship between root biomass and total root length using both phenotyping methods. Conclusion Overall, we developed a rhizobox pipeline for phenotyping the root system architecture of chile pepper plants under both well-watered and water-deficit conditions. We showed that measurements taken via non-destructive in-box imaging strongly predict those taken directly on washed scanned roots, with the added benefit of allowing repeated measurements over time.
Why it matches plant phenotyping methods根系表現型取得のためのrhizobox画像化パイプラインを開発し、非破壊画像法を洗浄根スキャン法と比較検証しており、フェノタイピング手法が研究の中心である。
abstractThe objectives of this study were to establish a method to grow chili pepper plants in a soil-based rhizobox container under water deficit conditions and compare two methods for collecting two-dimensional root trait data from their roots.
Accurate monitoring of crop phenotypic traits is essential for efficient farm management and automation in agriculture. Multi-object tracking (MOT) and video instance segmentation (VIS) offer promising approaches to enhance agricultural robotic-vision systems, yet a major limitation is the scarcity of high-quality spatial-temporal datasets. In this paper, we introduce BUP-ST20, a novel weakly labelled spatial-temporal dataset for sweet pepper tracking and segmentation captured on a robotic platform. Our dataset is generated by leveraging still image annotations and utilizing a neural radiance field approach (PAg-NeRF) to automatically obtain consistent object semantics and identities across video sequences. BUP-ST20 contains 16,240 images from 275 sequences, with weak labels for training and validation, and human-annotated ground truth for evaluation. We describe how this pseudo-labelling approach can be adapted to any robotic platform with the required inputs, greatly reducing the annotation requirements for dataset creation, with a focus on agriculture and horticulture. Utilizing BUP-ST20, we evaluate state-of-the-art MOT approaches and propose two novel tracklet matching criteria, enhancing robustness in frame-skipped scenarios and low frame rate cameras. When we decrease the frame rate to approximately 1 frame per second our offline MOT based matching criteria is able to improve performance by an absolute value of 19.63, outlining its validity as a tracklet aggregation technique in this scenario. Our experiments demonstrate the effectiveness of the dataset in benchmarking MOT and VIS techniques within the agricultural domain. This also allows us to highlight challenges such as occlusion, shape variations, and weak-labelling limitations. BUP-ST20 serves as a valuable resource for further advancements in robotic crop monitoring and agricultural automation, while demonstrating the ability to create future weakly labelled datasets using robotic platforms.
Why it matches plant phenotyping methods植物の追跡・セグメンテーションを対象とする時空間データセットを開発し、ロボット撮像、弱ラベル生成、ベンチマークまで扱っており、植物フェノタイピング手法が中心である。
abstractOur experiments demonstrate the effectiveness of the dataset in benchmarking MOT and VIS techniques within the agricultural domain.
Accurate characterization of chili pepper morphology is essential for breeding programs and genetic studies. Traditional phenotyping approaches are often constrained by small sample sizes and a limited set of measurable traits, restricting comprehensive analysis. In this study, we present an automated, image-based phenotyping framework that leverages computer vision and machine learning to extract detailed morphological features from longitudinal slice images of chili peppers. To accurately detect chili fruits and their seeds, the framework employs the YOLOv7 object detection model, achieving a precision of 0.92 and a mean Average Precision (mAP) of 0.87. Building upon these detections, we apply advanced image processing techniques to quantify key phenotypic traits, including seed count, fruit color intensity, length, width, surface area, and surface wrinkle characteristics. These parameters provide critical insights for variety classification, breeding selection, and genetic resource management. The proposed methodology not only enables scalable and reproducible phenotypic assessment but also establishes a searchable dataset of chili pepper varieties, thereby enhancing the efficiency, accuracy, and analytical depth of chili pepper research and breeding programs.
Why it matches plant phenotyping methods画像と機械学習を用いてトウガラシ果実・種子の形態形質を抽出する枠組みが研究の中心であり、実測精度も評価しているため。
abstractwe present an automated, image-based phenotyping framework that leverages computer vision and machine learning to extract detailed morphological features from longitudinal slice images of chili peppers.
As a vegetable crop with high economic value, the yield of pepper is often significantly restricted by leaf diseases, and the spots formed by these diseases on the surface of leaves are highly complex in color and texture characteristics. To overcome the shortcomings of traditional manual identification methods, such as low efficiency, time-consuming, and labor-consuming, an integrated multi-network model (IMNM) was established by combining an improved ResNet, a dynamic convolution network (DCN), and a progressive prototype network (PPN), which was aimed at five typical pepper leaf samples (healthy, virus, leaf blight, brown spot, and phyllosticta). The experimental results show that IMNM achieves 98.55% accuracy in pepper disease identification, which is significantly better than the benchmark models such as Inception-V4, ShuffleNet-V3, and EfficientNet-B7. In the cross-species generalization verification, the average identification accuracy of the model for apple, wheat, and rice leaf diseases increased to 99.81%, and its four core indicators of specificity, precision, sensitivity, and accuracy were all stable over 98%. This demonstrates that IMNM can effectively analyze the color and texture characteristics of highly heterogeneous disease spots and possesses strong cross-crop generalization capabilities. Its technical path lays a theoretical foundation for the development of field mobile disease diagnosis equipment based on deep learning, and is of great value for promoting the engineering application of an intelligent monitoring system for crop diseases and insect pests.
Why it matches plant phenotyping methods葉表の病斑を画像から識別する深層学習モデルを開発・比較評価しており、植物病害状態の取得・推定手法が中心である。
abstractan integrated multi-network model (IMNM) was established by combining an improved ResNet, a dynamic convolution network (DCN), and a progressive prototype network (PPN)
This article is coined for investigating the Village Plant dataset. Many researchers worldwide, carrying out their research in the domain of agriculture, are dependent on this open source dataset. A plant is vulnerable to several infirmities during its period of growth. Detection of the plant’s ill health and monitoring the environmental parameters is the most challenging task in agriculture. Plant disease epidemic may have a significant effect on crop production, reducing the country’s wealth. Early diagnosis of the occurrence of ill health in plants and the remedies are feasible using Artificial Intelligence (AI). Currently, methods like Deep Learning (DL) algorithms, machine vision techniques, and robotics play an important role in monitoring plant diseases and the growth status. This dataset contains multi-fold in-information about the plants. They include the normal and diseased images of plants like Bell Pepper, Tomato, Cucumber, and Potato. An Internet of Things (IoT) based plant data collection and integration system will provide data for this research, which optimizes the feature set through Ant Colony Optimization (ACO) for improving prediction in feature selection using deep learning models like DenseNet, ResNet 50, VGG 19, and Long Short-Term Memory (LSTM) networks, which in turn enhances plant productivity with advances in AI-driven agricultural diagnostics for plant stress prediction.
Why it matches plant phenotyping methods植物の正常・罹病画像から植物の病気・ストレス状態を推定する画像解析ワークフローとデータセット利用が研究の中心であり、植物状態のフェノタイピング手法に該当する。
abstractThis dataset contains multi-fold in-information about the plants. They include the normal and diseased images of plants like Bell Pepper, Tomato, Cucumber, and Potato.
Pepper / chilliObject detectionGrowth / development / phenology
Abstract has not been obtained from indexed metadata or an accessible article page.
Why it matches plant phenotyping methods唐辛子の成熟度という植物状態を画像認識で推定するAI手法の開発が題名の中心であり、植物フェノタイピング手法に該当する。
titleEV2M-YOLOv8: enhancing AI computer vision with EfficientNetV2 and multi-head self-attention for low-complexity agricultural crop, chilli maturity detection
Fruit shape significantly impacts the quality and market value of chili peppers (Capsicum annuum). However, predicting their fruit shapes in F₁ hybrids remains challenging, often relying on skilled breeders. This study aimed to clarify the potential of elliptic Fourier descriptors (EFDs) to predict fruit shape of F₁ progeny in chili peppers based on parental data. Using images of 291 accessions (132 inbred and 159 F₁ from 20 parental inbreds), EFDs were extracted to reconstruct shape contours. The initial prediction method, PPₘᵢd, used midpoint EFDs of the parents, achieving accuracies comparable to genomic methods. To improve accuracy, a new method, PPδ, was developed. PPδ incorporates dominance effects observed in F₁ progeny, yielding significantly better predictions. Over 80% of F₁ accessions showed improved accuracy with PPδ, and the predicted contours aligned closely with real shapes. Cross-validation confirmed the reproducibility of PPδ predictions. These findings suggest that combining parental EFDs with dominance effect ratios enables accurate fruit shape predictions without genetic data. This is the first study demonstrating EFD applicability in F₁ hybrid breeding for fruit shape, offering a promising tool for developing innovative breeding techniques in chili peppers.
Why it matches plant phenotyping methods画像から抽出した楕円フーリエ記述子を用いてトウガラシ果実形状を予測する新手法PPδを開発し、交差検証で再現性を評価しており、果実形状フェノタイピング手法が研究の中心である。
abstractTo improve accuracy, a new method, PPδ, was developed.
Root hair counting is a specialized aspect of plant biology and agronomy research that offers valuable insights into plant health, nutrient uptake, and overall growth potential. Root hairs are tiny extensions from the root epidermis that significantly increase the surface area and constitute roughly 70% of the total root area of a plant root system, enhancing the ability of plants to absorb water and nutrients from the soil. Understanding the importance of root hair counting involves looking at various aspects of plant physiology and soil–plant interactions. Despite these benefits, counting root hairs, especially manually, can be tedious, time-consuming, and, more often, inaccurate due to differences in the perception of individuals. Therefore, we have proposed a novel method for root hair counting and further observed an improvement in root hair count measurements when utilizing image super-resolution as a preprocessing step. Our approach of counting root hairs can tackle real-world challenges and be able to count overlapping hairs as well. By visualizing the rhizosphere in binary space, we can see a considerable increase in root hair count from 37 to 68 when counting manually to our approach for Bell pepper, and from 44 to 88, when counting manually to our method for Arabidopsis root images. To the best of the authors’ knowledge, this research study is specifically designed for root hair counting and measurement improvement using super-resolution, is the first of its kind, and has yet to be acknowledged.
Why it matches plant phenotyping methods植物の根毛数という形態形質を画像から自動計測する新規手法を開発し、超解像前処理による測定改善も検証しており、フェノタイピング手法が中心である。
titleA Novel Approach for Plant Root Hair Counting and its Improvement via Image Super-Resolution
Plant diseases significantly harm crops, resulting in significant economic losses across the globe. In order to reduce the harm that these diseases produce, plant diseases must be diagnosed accurately and timely manner. In this work, a YOLO-LeafNet approach is proposed for detecting diseases from leaf images of four distinct species, namely, grape, bell pepper, corn, and potato. About 8850 leaf images have been acquired for this work from five different publicly available datasets on Kaggle. All the acquired images were pre-processed by applying four different image pre-processing operations. The number of images in the training dataset was tripled for better model performance by applying five different augmentation operations. The augmented dataset was then used to train YoloV5, YoloV8, and the proposed YOLO-LeafNet. The performance of all three models is evaluated in terms of recall, precision, and Mean Average Precision (mAP). The YoloV5 attained a precision of 0.861, recall of 0.868, mAP50 of 0.944, and 0.815 of mAP50-95, and YoloV8 attained 0.977 precision, 0.975 recall, 0.984 of mAP50, and mAP50-95 of 0.915, whereas the proposed the YOLO-LeafNet attained precision of 0.985, recall of 0.980, mAP50 of 0.990, and mAP50-95 of 0.940. The experimental results reveal that the proposed YOLO-LeafNet outperformed YOLOv5 and YOLOv8 in terms of all performance metrics.
Why it matches plant phenotyping methods葉画像から植物病害を検出する深層学習手法を開発し、既存モデルと性能比較しており、植物状態の画像ベース表現型取得が中心である。
abstractIn this work, a YOLO-LeafNet approach is proposed for detecting diseases from leaf images of four distinct species, namely, grape, bell pepper, corn, and potato.
Aiming to address the problems of asynchronous acquisition time of multiple sensors in the crop phenotype acquisition system and high cost of the acquisition equipment, this paper developed a low-cost crop phenotype synchronous acquisition system based on the PTP synchronization protocol, realizing the synchronous acquisition of three types of crop data: visible light images, thermal infrared images, and laser point clouds. The paper innovatively proposed the Difference Structural Similarity Index Measure (DSSIM) index, combined with statistical indicators (average point number difference, average coordinate error), distribution characteristic indicators (Charm distance), and Hausdorff distance to characterize the stability of the system. After 72 consecutive hours of synchronization testing on the timing boards, it was verified that the root mean square error of the synchronization time for each timing board reached the ns level. The synchronous trigger acquisition time for crop parameters under time synchronization was controlled at the microsecond level. Using pepper as the crop sample, 133 consecutive acquisitions were conducted. The acquisition success rate for the three phenotypic data types of pepper samples was 100%, with a DSSIM of approximately 0.96. The average point number difference and average coordinate error were both about 3%, while the Charm distance and Hausdorff distance were only 1.14 mm and 5 mm. This system can provide hardware support for multi-parameter acquisition and data registration in the fast mobile crop phenotype platform, laying a reliable data foundation for crop growth monitoring, intelligent yield analysis, and prediction.
Why it matches plant phenotyping methods作物表現型取得のための低コスト・高速・時刻同期システムを開発し、可視画像、熱画像、レーザ点群の取得性能と安定性を検証しており、表現型取得法が中心である。
abstractthis paper developed a low-cost crop phenotype synchronous acquisition system based on the PTP synchronization protocol
Leaf area index (LAI) of chili peppers is an important indicator of plant growth and productivity. Accurate monitoring of LAI is crucial for optimizing growth conditions, and improving crop yields. However, existing remote sensing-based methods for LAI prediction often face low accuracy, especially in high-density vegetation and across different growth stages. These limitations primarily stem from issues such as multicollinearity and the constraints of spectral information processing mechanisms, which affect the stability and accuracy of predictions. To address these challenges, this study proposed a novel prediction method combining a tree-structured parzen estimator (TPE)-optimized two-band vegetation indices (TPE-2BVIs) with random forest regression (RFR). The TPE-2BVIs band optimization algorithm effectively extracts latent spectral information, enhancing LAI prediction stability and accuracy by addressing multicollinearity and nonlinear model complexity. Experimental results show that: (1) hyperspectral images provide more detailed spectral information compared to multispectral images, significantly improving the accuracy of LAI prediction; (2) the use of 2BVIs alleviates the effects of spectral saturation and dynamic variations during different growth stages, further improving prediction accuracy; (3) the proposed TPE-2BVIs band optimization method significantly enhances both the performance and stability of the model. When combined with RFR, the model achieves R² = 0.887, RMSE = 0.520, and NRMSE = 7.554 %. The TPE-2BVIs band optimization algorithm introduced in this study effectively extracts latent spectral information, overcoming the limitations of multicollinearity and the complexity of nonlinear models in traditional methods. This approach significantly improves the stability and accuracy of LAI predictions. The proposed method provides an innovative solution for remote sensing vegetation monitoring and agricultural applications, offering broad potential for estimating phenotypic parameters under diverse environmental conditions.
Why it matches plant phenotyping methodsUAVハイパースペクトル画像から植物形質であるLAIを推定する新規バンド最適化・回帰手法を開発し、精度と安定性を実験的に評価しているため、植物フェノタイピング手法が中心である。
abstractthis study proposed a novel prediction method combining a tree-structured parzen estimator (TPE)-optimized two-band vegetation indices (TPE-2BVIs) with random forest regression (RFR).
Computer vision heavily relies on features, especially in image classification tasks using feature-based architectures. Dimensionality reduction techniques are employed to enhance computational performance by reducing the dimensionality of inner layers. Convolutional Neural Networks (CNNs), originally designed to recognize critical image components, now learn features across multiple layers. Bidirectional LSTM (BiLSTM) networks store data in both forward and backward directions, while traditional Long Short-Term Memory (LSTM) networks handle data in a specific order. This study proposes a computer vision system that integrates BiLSTM with CNN features for image categorization tasks. The system effectively reduces feature dimensionality using learned features, addressing the high dimensionality problem in leaf image data and enabling early, accurate disease identification. Utilizing CNNs for feature extraction and BiLSTM networks for temporal dependency capture, the method incorporates label information as constraints, leading to more discriminative features for disease classification. Tested on datasets of pepper and maize leaf images, the method achieved a 99.37% classification accuracy, outperforming existing dimensionality reduction techniques. This cost-effective approach can be integrated into precision agriculture systems, facilitating automated disease detection and monitoring, thereby enhancing crop yields and promoting sustainable farming practices. The proposed Efficient Labelled Feature Dimensionality Reduction utilizing CNN-BiLSTM (ELFDR-LDC-CNN-BiLSTM) model is compared to current models to show its effectiveness in reducing extracted features for leaf detection and classification tasks.
Why it matches plant phenotyping methods葉画像から植物病害状態を推定するCNN-BiLSTM画像解析手法の開発・比較が中心であり、植物フェノタイピング手法に該当する。
abstractThis study proposes a computer vision system that integrates BiLSTM with CNN features for image categorization tasks.
SUMMARY Monitoring endogenous nutrient levels is crucial for maximizing crop yields and optimizing fertilizer use. Here, focusing on phosphorus, an essential nutrient for plant growth, we developed a low-cost and non-invasive biosensor to visualize and predict early stress signaling in plants. By combining plant phosphate (Pi)-deficiency-induced promoter systems with fungal self-sustained bioluminescence systems genetically engineered into tobacco plants, we created sensor plants that emitted more light when experiencing Pi deficiency. This light emission correlated with the expressions of known phosphate-responsive genes and the total phosphorus content in plants, and decreased during Pi recovery conditions, demonstrating the responsiveness and robustness of the sensor plants in reflecting endogenous phosphorus deficiency. The sensor plants responded primarily to Pi deficiency rather than nitrogen or potassium deficiencies and were sensitive to different ranges of external Pi concentrations. Additionally, when grafted onto tomato and chili pepper plants, the sensor plants responded to external phosphorus deficiency, showing promise for monitoring stress signals in different crop species. Using deep-learning-based image analysis techniques, auto-luminescent signals of sensor plants could be detected and used to predict phosphorus deficiency. This study outlines a strategy of creating a self-luminous biosensor to visualize phosphate dynamics in planta and predict nutrient deficiency for sustainable agriculture.
Why it matches plant phenotyping methods植物内リン欠乏状態を自己発光センサーと画像解析で可視化・予測する手法を開発し、応答性・頑健性を検証しているため、植物フェノタイピング手法が中心です。
abstractwe developed a low-cost and non-invasive biosensor to visualize and predict early stress signaling in plants
An accurate yet rapid assessment of pungency and color values is pivotal for the commercial processing of pepper, underscoring the critical need for high-throughput detection techniques. This study investigates a rapid method for determining the pungency and color value of paprika via LED-induced fluorescence spectroscopy. A simultaneous detection instrument was developed with a proposed fluorescence correction method to reduce the impact of excitation light reflected by the sample. The corrected spectra could improve the performance of pungency prediction. The best pungency model achieved a R c 2 of 0.941, R cv 2 of 0.935, RMSEC of 6813.41 SHU and RMSECV of 6959.97 SHU. The best color value model achieved an R c 2 of 0.862, R cv 2 of 0.822 RMSEC of 1.233 and RMSECV of 1.402. This study offers a practical solution for simultaneous quality assessment suitable for industrial deployment, with the potential to extend this methodology to diverse cultivars.
Why it matches plant phenotyping methodsLED誘起蛍光分光法による paprika の辛味・色価という植物器官形質の同時推定装置を開発し、補正法と予測性能を検証しており、表現型取得法が中心である。
abstractThis study investigates a rapid method for determining the pungency and color value of paprika via LED-induced fluorescence spectroscopy.
Chili plant diseases significantly impact global agriculture, necessitating accurate and rapid classification for effective management. The study introduces VGG-EffAttnNet, a hybrid deep learning model combining VGG16 and EfficientNetB0 with attention mechanisms and Monte Carlo Dropout (MCD) for robust chili plant disease classification. VGG16 captures spatial and hierarchical features, while EfficientNetB0 ensures efficient, high-accuracy learning. Attention enhances focus on disease-relevant areas, and MCD improves robustness by estimating uncertainty. The study utilizes a chili plant disease dataset sourced from Kaggle, comprising 5000 images across five classes: Healthy, Leaf Curl, Leaf Spot, Whitefly, and Yellowish, after extensive data augmentation techniques, including rotation, flipping, zooming, and brightness adjustment, to improve model generalization. Feature extraction is performed using VGG16 and EfficientNetB0, followed by concatenation and refinement through attention mechanisms, enabling the model to focus on disease-relevant features while suppressing background noise. MCD is integrated to estimate model uncertainty and mitigate overfitting. Experimental results demonstrate the superior performance of the proposed hybrid model. The concatenated VGG16 and EfficientNetB0 model achieved a classification accuracy of 99%, precision, and recall of 99%, surpassing individual model performances (VGG16: 96.8%, EfficientNetB0: 96.5%, and attention-integrated variants reached up to 98%). The F1-score reached 99% across all disease categories, ensuring high precision and recall. Compared to state-of-the-art models like InceptionV3 (98.83%) and MobileNet (97.18%), the proposed hybrid model demonstrates improved classification accuracy and robustness. The study underscores the potential of deep learning-based automated disease classification in precision agriculture, enabling early intervention and reducing reliance on chemical treatments. Future work aims to extend the approach to real-time deployment on mobile and edge devices, integrate explainability techniques for enhanced interpretability, and explore federated learning for decentralized agricultural diagnostics.
Why it matches plant phenotyping methods植物画像から病徴・健全状態を分類する深層学習手法が研究の中心であり、植物病害状態の画像ベース表現型推定に該当する。
abstractThe study introduces VGG-EffAttnNet, a hybrid deep learning model combining VGG16 and EfficientNetB0 with attention mechanisms and Monte Carlo Dropout (MCD) for robust chili plant disease classification.
Reproduction assets foundThe paper's chili plant disease image dataset (500 images, 5 classes, augmented to 5000) is explicitly stated to be publicly available on Kaggle, with a direct URL in the Data Availability Statement. No author analysis code or trained model checkpoints are reported as publicly available.Dataset · publicThe dataset used in this study is publicly available on Kaggle: https://www.kaggle.com/datasets/dhenyd/chili‐plant‐disease .Open asset ↗Kagglelines:927-975Plant phenotyping relevance match · UnverifiedCrossref · checked 14 Sept 2026
Ultrasonic sensing technology can contribute significantly to improving smart agricultural practices by recognizing plants and land features. Accurate detection of these field features is essential for the development of unmanned vehicles, which require precision navigation, obstacle avoidance, and successful field operation. Therefore, the objectives of the study were to employ ultrasonic sensors to detect key parameters of pepper plants and land features, specifically plant height, canopy volume, row spacing, and ridge spacing. Row spacing is the space between rows of plants, and ridge features are the raised soil beds that are often made for planting in upland farming systems. A data collection device was developed and tested in both laboratory and open-field environments. Initially, laboratory tests were conducted to evaluate the sensor accuracy of pepper plant height and canopy volume detection. Following successful validation, field trials were carried out in a pepper cultivation area using a remote-controlled vehicle platform to measure plant height, canopy volume, and row and ridge spacing. An open-source application was used to collect data and visualize the outcomes in real-time. The algorithm presented in the study effectively estimated the height, canopy volume, row spacing, and ridge spacing for pepper plants and associated land features. The results showed plant height of 61.34 and 61.49 cm, canopy volume of 0.29 and 0.31 m³, ridge spacing of 28.88 and 28.94 cm, and row spacing of 44.42 and 43.88 cm, respectively. No significant differences (p>0.05) were found between the measured and estimated plant and land features. Estimation values were strongly correlated with the measured values, with simple linear coefficients of determination (r2) of 0.95, 0.93, 0.88, and 0.81 for height, canopy volume, row spacing, and ridge spacing, respectively. The RMSE of these measurements ranged from 0.93 to 2.08 cm, highlighting relatively high accuracy of the proposed methods. The developed system shows the potential of ultrasonic sensors to develop automatic crop monitoring systems and support smart crop production and be adaptable to greenhouses, open fields or on-farm vehicles to identify different types of plants and land features.
Why it matches plant phenotyping methods超音波センサーと車載データ収集システムを開発し、コショウ植物の草丈・樹冠体積を検出・推定して実測値と検証しており、植物形質取得法が研究の中心である。
abstractA data collection device was developed and tested in both laboratory and open-field environments.
Abstract Ensuring food security is a crucial priority for nations worldwide, but plant diseases significantly hinder this objective through their impacts on agricultural productivity. Chili pepper (Capsicum spp.) is a major crop in West Africa, including in Benin. However, its production is challenged by diseases such as anthracnose and Tomato Yellow Leaf Curl Virus (TYLCV), which severely impact yields and farmer livelihoods. While traditional methods for disease detection and management have been commonly used, they are no longer sufficient to combat rising pest infestations and declining agricultural productivity. To tackle this issue, we built a comprehensive dataset of 213 images of anthracnose-affected leaves, 202 images of TYLCV-infected leaves, and 119 images of healthy leaves, collected under diverse environmental conditions in Benin. The study compared the performance of thirteen (13) deep learning models, including YOLOv8, MobileNetV2, and DenseNet121, for the classification of chili diseases using transfer learning techniques. Performance was evaluated using metrics such as Accuracy, Precision, Recall, and F1-Score. Results show that YOLOv8 outperformed other models in real-time detection and localization of leaf diseases, achieving a mean Average Precision (mAP@0.5) of 0.995 and mAP@0.5-0.95 of 0.941, with precision and recall exceeding 99%. Among CNN models, MobileNetV2 and DenseNet121 achieved 96.25% accuracy. These findings demonstrate that deep learning models, particularly YOLOv8, hold immense potential for real-time, automated detection of chili pepper diseases. Future research should focus on expanding datasets, integrating climatic variations, and improving disease severity assessment for enhanced agricultural sustainability.
Why it matches plant phenotyping methodsトウガラシ葉の病徴を画像から分類・検出する深層学習手法を開発・比較検証しており、植物の病害状態のフェノタイピングが研究の中心です。
abstractwe built a comprehensive dataset of 213 images of anthracnose-affected leaves, 202 images of TYLCV-infected leaves, and 119 images of healthy leaves
In order to characterize plant water deficiencies, this paper presents a custom-developed bioimpedance (BIS) measurement setup designed for in vivo studies that extracts plant leaf parameters using a novel optimization approach based on the particle swarm optimization (PSO) algorithm. The system performs, four-electrode measurements on plant leaves and employs a custom multi-objective cost function to validate parameters for the Double Shell Cole-Cole model. The experiment consisted of two parts: first, pepper plants (Capsicum annuum L.) as a model plant were exposed to drought stress in a light chamber, and their impedance and physiological parameters were measured. In the second part of the experiment, detached pepper leaves were allowed to dry naturally, and impedance measurements were recorded at hourly and tri-hourly intervals. Impedance spectrum measurements from 230 samples (1 Hz to 100 kHz), collected during both experiments, demonstrated that extracellular fluid resistance increases linearly with water loss. The proposed PSO-optimized Double Shell model showed a stronger correlation between extracellular fluid resistance and water loss compared to the widely used Zfit algorithm, which exhibited higher coefficient of variation in the Cole-Cole parameters. Both algorithms showed a significant negative correlation between relative water content and extracellular fluid resistance, but only the proposed PSO-based model detected a relationship between cell membrane capacity and membrane stability index. Additionally, extracellular fluid resistance correlated with photosynthetic efficiency. The results highlight the effectiveness of impedance measurements for assessing plant water status and support the reliability of proposed PSO-based optimization for bioimpedance analysis.
Why it matches plant phenotyping methods植物の水分状態を測定するバイオインピーダンス計測系とPSO最適化モデルを開発・比較検証しており、植物フェノタイプ取得手法が研究の中心である。
abstractthis paper presents a custom-developed bioimpedance (BIS) measurement setup designed for in vivo studies that extracts plant leaf parameters using a novel optimization approach based on the particle swarm optimization (PSO) algorithm.
The accurate assessment of plant disease severity is crucial for effective crop management. Deep learning, especially via CNNs, is widely used for image segmentation in plant lesion detection, but accurately assessing disease severity across varied environmental conditions remains challenging. This study evaluates eight deep learning models for detecting and quantifying Cercospora leaf spot ( Cercospora capsici ) severity in chili peppers under natural field conditions. A custom dataset of 1645 chili pepper leaf images, collected from a Brazilian plantation and annotated with 6282 lesions, was developed for real-world robustness, reflecting real-world variability in lighting and background. First, an algorithm was developed to process raw images, applying ROI selection and background removal. Then, four YOLOv8 and four Mask R-CNN models were fine-tuned for pixel-level segmentation and severity classification, comparing one-stage and two-stage models to offer practical insights for agricultural applications. In pixel-level segmentation on the test dataset, Mask R-CNN achieved superior precision with a Mean Intersection over Union (MIoU) of 0.860 and F1-score of 0.924 for the mask_rcnn_R101_FPN_3x model, compared to 0.808 and 0.893 for the YOLOv8s-Seg model. However, in severity classification, Mask R-CNN underestimated higher severity levels, with an accuracy of 72.3% for level III, while YOLOv8 attained 91.4%. Additionally, YOLOv8 demonstrated greater efficiency, with an inference time of 27 ms versus 89 ms for Mask R-CNN. While Mask R-CNN excels in segmentation accuracy, YOLOv8 offers a compelling balance of speed and reliable severity classification, making it suitable for real-time plant disease assessment in agricultural applications.
Why it matches plant phenotyping methods植物病斑の検出・重症度定量化を目的とする画像解析手法を開発し、複数モデルの精度・速度・分類性能を比較検証しており、植物表現型取得が中心である。
abstractA custom dataset of 1645 chili pepper leaf images, collected from a Brazilian plantation and annotated with 6282 lesions, was developed for real-world robustness
The object of the study is the forecasting and optimizing the plant growth rather. The data distribution at each iteration in the continuous optimization process tends to produce premature convergence because the optimum points are found at the beginning of the iteration, so that the actual optimum condition cannot be achieved. For this reason, a method is needed to see the optimum points at each iteration in the continuous optimization process. A multi-linear regression approach is used to predict the variables generated at each iteration, and then optimized using a neural network method approach for each optimum point found. This research is implemented and observed on the growth morphology of chili plants with a total sample of 100 stems, for 100 days of growth. The testing process consists of 5 different experimental scenarios based on the activation function, and the iteration process is carried out at 250, 500, and 1000 epochs. Furthermore, with a percentage of 70% training data and 30% testing data, the results obtained using the ReLU activation function have an ideal value compared to the Tanh, Softplus, Elu, and Sigmoid activation functions. Compared to the time series method with an MSE value of 4.62, this value is much better than the value of 8.6 for the time series. The RMSE and MAPE values of 16.36 and 36.53 are also excellent. Comparison of the level of forecasting accuracy of the results of continuous optimization carried out with the activation function ReLU and tanh compared to the time series method, the value with the activation function ReLU and tanh has a percentage value 46.36% and 46.86% and this value is a good value compared to using the time series method, which is exactly 67.39%
Why it matches plant phenotyping methods植物の成長形態を予測・最適化する機械学習手法が研究の中心であり、観測可能な植物形態を推定対象として技術比較・評価しているため。
abstractA multi-linear regression approach is used to predict the variables generated at each iteration, and then optimized using a neural network method approach for each optimum point found.
As an emerging vegetable cultivation technology, plug seedling cultivation significantly improves seedling production efficiency and reduces costs through standardization. Grading and transplanting, as the final step before the sale of plug seedlings, categorizes seedlings into different grades to ensure consistent quality. However, most current grading methods can only detect seedling emergence but cannot classify the emerged seedlings. Therefore, this study proposes an intelligent grading method for pepper plug seedlings based on RGB and point cloud images, enabling precise grading using both RGB and 3D point cloud data. The proposed method involves the following steps: First, RGB and point cloud images of the seedlings are acquired using 2D and 3D cameras. The point cloud data is then converted into a 2D representation and aligned with the RGB images. Next, a deep learning-based object detection algorithm identifies the positions of individual seedlings in the RGB images. Using these positions, the seedlings are segmented from both the RGB and 2D point cloud images. Subsequently, a deep learning-based leaf recognition algorithm processes the segmented RGB images to determine leaf count, while another deep learning-based algorithm segments the leaves in the 2D point cloud images to extract their spatial information. Their surface area is measured using 3D reconstruction method to calculate leaf area. Additionally, plant height is derived from the point cloud’s height data. Finally, a classification model is trained using these extracted features to establish a grading system. Experimental results demonstrate that this automated grading method achieves a success rate of 97%, and compared with manual methods, this method has higher production efficiency. Meanwhile, it can grade different tray seedlings by training different models and provide reliable technical support for the quality evaluation of seedlings in industrialized transplanting production.
Why it matches plant phenotyping methodsRGB画像と3D点群から葉数・葉面積・草丈を抽出し、苗の品質等級化を行う植物フェノタイピング手法が研究の中心である。
abstractthis study proposes an intelligent grading method for pepper plug seedlings based on RGB and point cloud images
Accurate assessment of plant disease severity is crucial for effective crop management. Deep learning, especially CNNs, is widely used for image segmentation in plant lesion detection, but accurately assessing disease severity across varied environmental conditions remains challenging. This study evaluates eight deep learning models for detecting and quantifying Cercospora leaf spot severity in chili peppers under natural field conditions. A custom dataset of 1,645 chili pepper leaf images, collected from a Brazilian plantation and annotated with 6,282 lesions, was developed for real-world robustness, reflecting real-world variability in lighting and background. First, an algorithm was developed to process raw images, applying ROI selection and background removal. Then, four YOLOv8 and four Mask R-CNN models were fine-tuned for pixel-level segmentation and severity classification, comparing one-stage and two-stage models to offer practical insights for agricultural applications. In pixel-level segmentation on the test dataset, Mask R-CNN achieved superior precision with a Mean Intersection over Union (MIoU) of 0.860 and F1-score of 0.924 for the mask_rcnn_R101_FPN_3x model, compared to 0.808 and 0.893 for the YOLOv8s-Seg model. However, in severity classification, Mask R-CNN underestimated higher severity levels, with an accuracy of 72.3% for level III, while YOLOv8 attained 91.4%. Additionally, YOLOv8 demonstrated greater efficiency, with an inference time of 27 ms versus 89 ms for Mask R-CNN. While Mask R-CNN excels in segmentation accuracy, YOLOv8 offers a compelling balance of speed and reliable severity classification, making it suitable for real-time plant disease assessment in agricultural applications.
Why it matches plant phenotyping methods植物病斑の画像セグメンテーションと病害重症度推定を開発・比較評価しており、植物表現型取得手法が研究の中心である。
abstractThis study evaluates eight deep learning models for detecting and quantifying Cercospora leaf spot severity in chili peppers under natural field conditions.
Abstract E arly disease detection is fundamental to protect the crops and provide early treatment. Plants with medicinal values are rare and need complete care. Black pepper is a spice herb, highly used as medicinal plant. The diseases prominent in black pepper spice are anthracnose, phytophthora, slow wilt, quick wilt, and yellowing. The proposed work suggests deep learning-based prediction and classification. The proposed work utilizes a benchmark dataset created in real time environment. The dataset is preprocessed, segmented, and labeled into classes under expert supervision. Deep neural network ResNet − 50 is trained with novice data. The trained features are saved in an array. This data is matched in linear with extracted feature of Support vector machine SVM, a machine learning algorithm used for disease prediction. This is done by passing and mapping the hyperparameter features of Renet-50 as an array and mapping it to feature array of multiclass support vector machine. Hence there is no requirement of training machine learning separately. This results in fast training and early disease prediction .
Why it matches plant phenotyping methods黒コショウ葉の病害症状を画像から分類・予測する深層学習/SVM手法と、前処理・セグメンテーション・ラベリング済みデータセットを中心に扱っており、植物病態の画像ベース表現型計測に該当する。
abstractThe proposed work suggests deep learning-based prediction and classification.
The primary objective of this study was to evaluate the reliability of chili leaf image classification as a method for distinguishing chili germplasm accessions, thereby supporting germplasm conservation efforts. Traditional methods for identifying chili varieties rely on manual leaf observation, which is labor-intensive and error-prone, highlighting the need for advanced fine-grained classification techniques. To address this challenge, we introduce a curated chili leaf database, JNUCLS, to support future research. We evaluate state-of-the-art deep learning methods for chili variety classification and propose a novel approach, LGENetB4CA, which combines a modified EfficientNetB4 model with a LeafGabor filter. The EfficientNetB4 incorporates a Coordinate Attention block following its layers to effectively capture both spatial and channel-wise information. This enables the model to focus on critical regions, such as fine-grained leaf textures, while maintaining a global context. The LeafGabor filter enhances intricate leaf details, such as vein structures, while suppressing noisesuch as blurred shadows, significantly improving input quality. Experiments on the JNUCLS and COLD chili datasets demonstrate high accuracy in chili variety and leaf disease classification, with LGENetB4CA achieving 89.61% accuracy on JNUCLS and 85.90% on COLD chili. These findings highlight the potential of leaf image classification as an effective, cost-efficient tool for exploring phenotypic diversity among chili cultivars. The proposed method also demonstrates promise for broader applications, including plant classification systems, targeted crop management, agricultural product tracking, market analysis, and biodiversity preservation.
Why it matches plant phenotyping methods葉画像から品種差と葉病害を分類する深層学習手法を開発・評価し、データセットも構築しているため、植物表現型取得・分類が中心的です。
abstractwe introduce a curated chili leaf database, JNUCLS, to support future research.
Despite the significant progress in deep learning-based object detection, existing models struggle to perform optimally in complex agricultural environments. To address these challenges, this study introduces YOLO-Pepper, an enhanced model designed specifically for greenhouse pepper disease and pest detection, overcoming three key obstacles: small target recognition, multi-scale feature extraction under occlusion, and real-time processing demands. Built upon YOLOv10n, YOLO-Pepper incorporates four major innovations: (1) an Adaptive Multi-Scale Feature Extraction (AMSFE) module that improves feature capture through multi-branch convolutions; (2) a Dynamic Feature Pyramid Network (DFPN) enabling context-aware feature fusion; (3) a specialized Small Detection Head (SDH) tailored for minute targets; and (4) an Inner-CIoU loss function that enhances localization accuracy by 18% compared to standard CIoU. Evaluated on a diverse dataset of 8046 annotated images, YOLO-Pepper achieves state-of-the-art performance, with 94.26% mAP@0.5 at 115.26 FPS, marking an 11.88 percentage point improvement over YOLOv10n (82.38% mAP@0.5) while maintaining a lightweight structure (2.51 M parameters, 5.15 MB model size) optimized for edge deployment. Comparative experiments highlight YOLO-Pepper's superiority over nine benchmark models, particularly in detecting small and occluded targets. By addressing computational inefficiencies and refining small object detection capabilities, YOLO-Pepper provides robust technical support for intelligent agricultural monitoring systems, making it a highly effective tool for early disease detection and integrated pest management in commercial greenhouse operations.
Why it matches plant phenotyping methodsコショウの病害を画像から検出する深層学習手法を開発し、注釈画像データセットと複数モデルで性能比較・検証しており、植物の病害状態の取得方法が中心です。害虫検出も含みますが、病害検出の技術的貢献が明確なため採用します。
abstractthis study introduces YOLO-Pepper, an enhanced model designed specifically for greenhouse pepper disease and pest detection
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Dataset · publicThe data can be accessed at https://data.mendeley.com/datasets/ disease? Adv Multimed. 2018;2018:6710865.Open asset ↗pdf-page:17 lines:1-54Plant phenotyping relevance match · UnverifiedEurope PMC · checked 6 Sept 2026
Monitoring the root system plays an important role in understanding plant physiological processes; however, its assessment using non-destructive methods remains challenging. Here, we evaluate the utility of root capacitance (C R ) as a practical indicator of root function and its relationship to plant growth parameters in Capsicum annuum L. To improve the accuracy of root function assessment, we applied artificial neural networks (ANN) as a novel data evaluation approach, comparing its predictive performance against multiple linear regression (MLR). Across two soil types (sandy and sandy loam), we applied multiple treatments ranging from microbial inoculants to wool pellet and inorganic nitrogen sources primarily to test whether C R could detect differences in root activity and biomass production under different conditions. We measured root dry biomass, shoot dry biomass, and leaf N content, treating these variables as independent predictors in a statistical framework. Multiple linear regression (MLR) initially showed strong relationship between C R and both root and shoot biomass in sandy soil, and between C R and total plant N content in sandy loam. However, an ANN model consistently outperformed MLR in predicting C R from plant physiological parameters, as evidenced by lower mean absolute error (MAE) in all treatments. These findings confirm that C R correlates strongly with plant growth parameters and can reliably distinguish the effects of different soil amendments even those with markedly different nutrient-release profiles.
Why it matches plant phenotyping methods根キャパシタンスを根機能・バイオマスの非破壊指標として評価し、ANNによる予測性能をMLRと比較しているため、処理試験にとどまらずフェノタイピング手法の実用性・精度評価が中心的です。
abstractwe evaluate the utility of root capacitance (C R ) as a practical indicator of root function and its relationship to plant growth parameters
Common beanPepper / chilliTomatoLeafClassificationStress / disease detectionDisease symptoms / severity
Sustainable agriculture holds the key in meeting food production requirements for a rapidly growing population without exacerbating environmental degradation. Plant leaf diseases pose a critical threat to crop yield and quality. Existing inspection methods are labor-intensive and prone to human errors, while lacking support for large-scale agriculture. This research aims to enhance plant health by developing advanced deep learning models for the detection and classification of plant diseases across a variety of species. A deep learning model based on the paradigm of the MobileNet architecture is proposed, which employs a dedicated design through deeper convolutional layers, dropout regularization, and fully connected layers. This results in significant improvements in disease classification in tomato, bean, and chili plants, with accuracy rates of 97.90%, 98.12%, and 97.95%, respectively. Moreover, Grad-CAM is used to shed light on the decision-making process of the proposed model. The work contributes to the advancement of precision farming and sustainable agricultural practices, supporting timely and accurate plant disease diagnosis.
Why it matches plant phenotyping methods植物葉の病害状態を画像から検出・分類する深層学習法の開発と評価が研究の中心であり、植物フェノタイピング手法に該当します。
abstractThis research aims to enhance plant health by developing advanced deep learning models for the detection and classification of plant diseases across a variety of species.
The effective acquisition of crop phenotypic structure information is crucial for monitoring and analyzing crop growth. However, in high-density agricultural environments, the complexity and redundancy of the data, coupled with the need for sweet pepper plants to be supported by surrounding frames and wires, significantly complicate the extraction of phenotypic structures. To address these challenges, this paper proposes an EGF-Former-based method for extracting phenotypic structures of sweet peppers in complex environments. The model's ability to capture fine structures is enhanced through the EfficientMod Module (EMM), which extracts information from multiple feature spaces. A refined attention allocation strategy, Global Masked Attention (GMA), is introduced to construct separate masks for foreground and background regions, allowing for accurate segmentation of these areas. Additionally, we propose a module based on the Fast Fourier Transform (FFTM), which improves local contour extraction by obtaining high-frequency information in the frequency domain and performing cross-domain fusion with spatial domain features. This approach significantly enhances the segmentation of edge-blurred phenotypic structures. Experimental results demonstrate that our method exhibits strong robustness compared to state-of-the-art techniques, achieving 83.45 % and 90.37 % in the mIoU and mAcc metrics, respectively. Compared to the baseline model, the segmentation mIoU score improves by up to 3.1 %, while the model’s parameter count is reduced by 10 %, greatly enhancing the usability of EGF-Former in complex agricultural environments.
Why it matches plant phenotyping methodsスイートペッパーの構造的表現型を画像から抽出するセグメンテーション手法を開発し、既存手法と比較評価しているため、植物表現型取得法が研究の中心である。
abstractthis paper proposes an EGF-Former-based method for extracting phenotypic structures of sweet peppers in complex environments
Introduction Pepper leaf segmentation plays a pivotal role in monitoring pepper leaf diseases across diverse backgrounds and ensuring healthy pepper growth. However, existing Transformer-based segmentation methods grapple with computational inefficiency, excessive parameterization, and inadequate utilization of edge information. Methods To address these challenges, this study introduces an Adaptive Multi-Scale MLP (AMS-MLP) framework. This framework integrates the Multi-Path Aggregation Module (MPAM) and the Multi-Scale Context Relation Mask Module (MCRD) to refine object boundaries in pepper leaf segmentation. The AMS-MLP includes an encoder, an Adaptive Multi-Scale MLP (AM-MLP) module, and a decoder. The encoder's MPAM fuses five-scale features for accurate boundary extraction. The AM-MLP splits features into global and local branches, with an adaptive attention mechanism balancing them. The decoder enhances boundary feature extraction using MCRD. Results To validate the proposed method, extensive experiments were conducted on three pepper leaf datasets with varying backgrounds. Results demonstrate mean Intersection over Union (mIoU) scores of 97.39%, 96.91%, and 97.91%, and F1 scores of 98.29%, 97.86%, and 98.51% across the datasets, respectively. Discussion Comparative analysis with U-Net and state-of-the-art models reveals that the proposed method significantly improves the accuracy and efficiency of pepper leaf image segmentation.
Why it matches plant phenotyping methodsコショウ葉画像のセグメンテーション手法を開発し、複数データセットで性能検証しており、植物器官の観測・抽出方法が研究の中心である。
abstractthis study introduces an Adaptive Multi-Scale MLP (AMS-MLP) framework
Aim: This study aimed to propose a computationally cost-effective method for automated estimation of plant leaf disease severity in resource-limited settings. Study Design: The performance of four image segmentation algorithms—global thresholding, adaptive thresholding, Otsu thresholding, and edge detection—was evaluated using nine curated images of disease-affected leaves from tomato, bell pepper, and potato plants. Each image was segmented into healthy and diseased regions, and quantitative metrics—including diseased pixel counts, percentage of affected area, healthy-to-diseased ratios, and computational time—were analyzed to assess algorithm performance. Results: The segmentation methods executed with near-instantaneous speed (0–0.001 seconds per image). Global and Otsu thresholding consistently demonstrated high segmentation accuracy, leading to reliable severity estimations. Adaptive thresholding tended to overestimate disease severity, while edge detection, despite providing precise lesion boundaries, significantly underestimated overall disease severity. Conclusion: Comparative analysis, supported by visual validation, suggests that Otsu thresholding, closely followed by global thresholding, is the most effective approach for leaf disease severity estimation, offering high accuracy with minimal computational overhead. These findings indicate that classical computer vision techniques can play a valuable role in supporting plant disease diagnostics and estimation in resource-constrained environments.
Why it matches plant phenotyping methods植物葉の病害重症度という観察可能な状態を、画像セグメンテーションで定量推定する手法を提案し、複数アルゴリズムを比較評価しているため、植物フェノタイピング手法が中心である。
abstractThis study aimed to propose a computationally cost-effective method for automated estimation of plant leaf disease severity in resource-limited settings.
Effective water management is crucial for ensuring the healthy growth and high yield of crops, and it relies on accurate monitoring of plant water status. As a core indicator of plant gas exchange capacity, stomatal conductance (Gs) directly determines the efficiency of photosynthesis and transpiration, significantly impacting crop growth and yield formation. Therefore, timely and accurate prediction of stomatal conductance is essential for optimizing water management strategies and improving crop yield and quality. However, stomatal conductance is influenced by a variety of environmental factors and plant physiological traits, making its variability complex and dynamic. These challenges result in difficulties in selecting key features, insufficient prediction accuracy, and a lack of transparency in model decision-making processes. To address these issues, this study proposes a novel approach that combines a random forest (RF) feature selection method with a Tree-structured Parzen Estimator (TPE)-optimized light gradient boosting machine (LightGBM) model (TPE-LightGBM). This approach leverages UAV-based hyperspectral, thermal infrared imagery, and meteorological data to improve predictive performance. Additionally, SHAP (SHapley Additive exPlanations) analysis is incorporated to offer insights into the model’s decision-making process by revealing feature dependencies. In the feature selection process, we compared four common methods, including mutual information (MI), successive projection algorithm (SPA), recursive feature elimination (RFE), and least absolute shrinkage and selection operator (LASSO) to ensure the significance and effectiveness of the selected features. To comprehensively evaluate model performance, we also compared five predictive models: ridge regression (RR), partial least squares regression (PLSR), random forest regression (RFR), random search-optimized LightGBM (Random-LightGBM), and grid search-optimized LightGBM (Grid-LightGBM). The experimental results revealed that the combination of RF and TPE-optimized LightGBM significantly outperformed all other models, achieving the highest prediction accuracy. The optimal number of features was determined to be N = 15, with a coefficient of determination (R²) of 0.862, a root mean square error (RMSE) of 0.037, and a mean absolute error (MAE) of 0.029. Through SHAP analysis, the study not only identifies key influencing factors such as photosynthetically active radiation (PAR), canopy temperature (CT), and red-edge spectral bands, but also sheds light on how these factors interact with each other to influence stomatal conductance. The proposed model provides an innovative approach to effectively predicting stomatal conductance, enabling agricultural managers to better understand and regulate chili pepper’s water status, thereby promoting healthy chili pepper growth and efficient resource management.
Why it matches plant phenotyping methodsUAVのハイパースペクトル・熱赤外画像からチリ pepper の気孔コンダクタンスという生理形質を推定するモデルを開発・比較・解釈しており、表現型取得・推定手法が研究の中心です。
abstractThis approach leverages UAV-based hyperspectral, thermal infrared imagery, and meteorological data to improve predictive performance.
• Chili pepper seedling responses to heat stress were analyzed under controlled environmental conditions. • Morphological and spectral traits were identified through non-destructive 3D imaging. • PCA and clustering revealed three distinct response patterns to heat stress among genotypes. • A novel PC distance-based approach quantified heat stress stability among genotypes. • The developed phenotyping method provides an efficient tool for large-scale heat tolerance screening in breeding programs. Climate change-driven heat stress presents a significant threat to global pepper production, highlighting the urgent need for efficient methods to assess heat tolerance in breeding programs. This study presents a robust approach for assessing heat stress responses in pepper integrating high-throughput phenotyping and multivariate analysis. Twenty pepper genotypes were evaluated under controlled temperature conditions (40/35 °C day/night) for 14 days using the TraitFinder system equipped a pair of 3D multispectral scanner. Principal component analysis (PCA) of morphological and spectral traits revealed progressive divergence between control and heat-treated groups, with the maximum separation observed at day 10 (ΔC = 2.05). Three distinct response groups were identified based on Euclidean distances in the PCA space: low response (five genotypes), moderate response (nine genotypes), and high response (six genotypes). The PC-based distance metric showed strong correlations with conventional stress tolerance indicators, including biomass retention ( r = 0.66) and root system maintenance ( r = 0.48). Notably, genotype 'Pep17 (GPC121710)' demonstrated enhanced growth under heat stress (26 % increase in 3D leaf area), while 'Pep06 (GPC003350)' showed marked growth reduction (27 % decrease). This study validated the integration of high-throughput phenotyping with PCA-based metrics for the quantitative assessment of heat stress responses. The method offers an efficient tool for identifying heat-tolerant pepper genotypes and holds potential for application to other crops and stress conditions, supporting climate resilience breeding programs.
Why it matches plant phenotyping methods3Dマルチスペクトル画像による高スループット形質取得と、PCA距離指標による耐暑性評価を中心に開発・検証しており、植物フェノタイピング手法が中核である。
abstractThis study presents a robust approach for assessing heat stress responses in pepper integrating high-throughput phenotyping and multivariate analysis.
Abstract Pepper, Hibiscus, and Basil are medicinal plants with a rich history in traditional medicine and health benefits. They are essential in culinary and medicinal applications, contributing to natural health solutions. Disease detection is crucial to protect their agricultural, economic, and medicinal value. Early detection minimizes crop losses, maintains plant health, and ensures plant availability for traditional medicine and culinary uses. This promotes sustainable and eco-friendly agricultural practices. Traditional logistic regression for plant leaf disease detection struggles with imbalanced data and a fixed linear decision boundary, making it less effective in capturing complex disease patterns. The modified logistic regression model with the One Half Constant improves performance metrics and handling intricate features of leaf images by addressing class imbalance more effectively. It adjusts the decision boundary to handle imbalanced datasets, enhancing classification accuracy for minority classes while maintaining simplicity and interpretability. This study uses a dataset collected from Kaggle and surrounding of Kadapa district AP, India. For the evaluation of the proposed model in disease detection, the traditional logistic regression and other machine learning algorithms were used, and the corresponding key metrics of accuracy, precision, recall, false positive rate (FPR) and F-Measure were assessed. A comparison with existing methods show overwhelming improvement of 32.94% in accuracy, 17.64% in precision, 33.6% in recall, 86.91% in FPR improvement, 34.6% in F-Measure. The proposed approach seeks to improve overall diagnostic accuracy, thereby providing a reliable tool for early detection and treatment planning in clinical sectors.
Why it matches plant phenotyping methods植物葉画像から病害状態を推定する分類手法を改良し、既存手法との性能比較で検証しているため、植物フェノタイピング手法が中心である。
titleEnhanced Plant Leaf Disease Detection Using Modified Logistic Regression for Sustainable Agriculture Practices
Reproduction assets foundThe paper states that the pepper leaf images used in its disease-detection experiments are publicly available in the 'Pepper disease classification' Kaggle repository, matching an allowed URL. Basil and hibiscus images were locally collected with no deposit, and no author analysis code or models are shared.Dataset · publicthe Pepper leaf images
utilized are available in “Pepper disease classification” repository:
https://www.kaggle.com/code/longqua69/pepper-disease-classification/output.Open asset ↗Kaggle · longqua69/pepper-disease-classificationpdf-page:22 lines:1-62Plant phenotyping relevance match · UnverifiedEurope PMC · checked 15 Sept 2026
Early diagnosis of plant diseases is essential for reducing crop losses and improving agricultural production. Plant diseases markedly diminish output and food supply, highlighting the critical necessity for effective diagnostic instruments. Leaf analysis is an effective technique for evaluating plant health, providing information on diseases and deficiencies. This research underscores the significance of machine learning (ML) in predicting plant diseases, utilising its capacity to analyse characteristics from leaf photos and categorize plants as healthy or ill. This study presents 35 prediction models that integrate seven leading machine learning techniques—Support Vector Machines (SVM), Random Forest (RF), Naïve Bayes (NB), Decision Tree (DT), k-Nearest Neighbor (KNN), Logistic Regression (LR) and Multilayer Perceptron (MLP)—with five crop varieties: maize, apple, grapes, tomato, and bell pepper. An equitable crop selection strategy guarantees the inclusion of grains, fruits, and vegetables. The PlantVillage dataset is employed for model training and testing, with performance assessed by measures such as Accuracy, Precision, Recall, F1-Measure, ROC, and AUC. The Random Forest method demonstrated superior efficacy, with the greatest accuracy (91.8%), F1-Measure (91.4%), and AUC score (96.2%), surpassing SVM, KNN, and MLP. The Friedman test provided statistical support, affirming the trustworthiness of these findings. This study enhances the comprehension of machine learning applications in agriculture and establishes a comprehensive framework for the early diagnosis of plant diseases, therefore contributing to food security and sustainable agricultural practices.
Why it matches plant phenotyping methods葉画像から植物の健全・罹病状態を推定する機械学習手法を比較・評価しており、病害表現型の取得・分類が研究の中心です。
abstractThis research underscores the significance of machine learning (ML) in predicting plant diseases, utilising its capacity to analyse characteristics from leaf photos and categorize plants as healthy or ill.
China is the world's largest producer of chili peppers, which occupy particularly important economic and social values in various fields such as medicine, food and industry.However, during its production process, chili peppers are affected by pests and diseases resulting in significant yield reduction due to temperature, environment and other reasons. In this study, a lightweight pepper disease identification method DD-YOLO based on the YOLOv8n model is proposed. First, the deformable convolutional module DCNv2 (Deformable Convolutional Networks) and the inverted residual mobile block iRMB (Inverted Residual Mobile Block) are introduced into the C2Fmodule to improve the accuracy of the sampling range and reduce the computational amount; secondly, the DySample sampling operator (Dynamic Sample) is integrated into the head network toreduce the amount of data and reduce the complexity of computation. Finally, we use Large Separable Kernel Attention (LSKA) to improve the SPPF module (Spatial Pyramid Pooling Fast) to enhance the performance of multi-scale feature fusion. The experimental results show that the accuracy, recall and average precision of the DD-YOLO model are 91.6%, 88.9% and 94.4%, respectively, compared with the base network YOLOv8n, it improves 6.2, 2.3 and 2.8 percentage points respectively, the model weight is reduced by 22.6%, and the number of floating-point operations per second is improved by 11.1%. This method provides a technical basis for intensive cultivation and management of chili peppers as well as efficiently and cost-effectively accomplishes the task of identifying chili pepper pests and diseases.
Why it matches plant phenotyping methods画像からトウガラシの病害状態を識別するYOLOベース手法を開発し、精度・再現率・計算量を比較評価しており、植物病害フェノタイピング手法が中心である。
abstracta lightweight pepper disease identification method DD-YOLO based on the YOLOv8n model is proposed.
The effective acquisition of crop phenotypic structure information is crucial for monitoring and analyzing crop growth. However, in high-density agricultural environments, the complexity and redundancy of the data, coupled with the need for sweet pepper plants to be supported by surrounding frames and wires, significantly complicate the extraction of phenotypic structures. To address these challenges, this paper proposes an EGF-Former-based method for extracting phenotypic structures of sweet peppers in complex environments. The model's ability to capture fine structures is enhanced through the EfficientMod Module (EMM), which extracts information from multiple feature spaces. A refined attention allocation strategy, Global Masked Attention (GMA), is introduced to construct separate masks for foreground and background regions, allowing for accurate segmentation of these areas. Additionally, we propose a module based on the Fast Fourier Transform (FFTM), which improves local contour extraction by obtaining high-frequency information in the frequency domain and performing cross-domain fusion with spatial domain features. This approach significantly enhances the segmentation of edge-blurred phenotypic structures. Experimental results demonstrate that our method exhibits strong robustness compared to state-of-the-art techniques, achieving 83.45 % and 90.37 % in the mIoU and mAcc metrics, respectively. Compared to the baseline model, the segmentation mIoU score improves by up to 3.1 %, while the model’s parameter count is reduced by 10 %, greatly enhancing the usability of EGF-Former in complex agricultural environments. • Proposes EGF-Former, a novel method for extracting sweet pepper phenotypic structures in complex backgrounds. • Develops FFTM, a high-frequency-based edge enhancement method to improve feature sensitivity. • Achieves significant parameter reduction, enabling applications on high-throughput phenotyping platforms.
Why it matches plant phenotyping methodsスイートペッパーの表現型構造を画像セグメンテーションで抽出する手法を開発し、複雑環境での性能評価と高スループット表現型プラットフォームへの適用性を示しており、表現型取得が中心的です。
abstractExperimental results demonstrate that our method exhibits strong robustness compared to state-of-the-art techniques
Volatile organic compounds (VOCs) are common constituents of fruits, vegetables, and crops, and are closely associated with their quality attributes, such as firmness, sugar level, ripeness, translucency, and pungency levels. While VOCs are vital for assessing vegetable quality and phenotypic classification, traditional detection methods, such as Gas Chromatography-Mass Spectrometry (GC-MS) and Proton Transfer Reaction Mass Spectrometry (PTR-MS) are limited by expensive equipment, complex sample preparation, and slow turnaround time. Additionally, the transient nature of VOCs complicates their detection using these methods. Here, we developed a paper-based colorimetric sensor array combined with needles that could: 1) induce vegetable VOC release in a minimally invasive fashion, and 2) analyze VOCs in situ with a smartphone reader device. The needle sampling device helped release specific VOCs from the studied vegetables that usually require mechanic stimulation, while maintaining the vegetable viability. On the other hand, the colorimetric sensor array was optimized for sulfur compound-based VOCs with a limit of detection (LOD) in the 1-25 ppm range, and classified fourteen different vegetable VOCs, including sulfoxides, sulfides, mercaptans, thiophenes, and aldehydes. By combining principal components analysis (PCA) analysis, the integrated sensor platform proficiently discriminated between four vegetable subtypes originating from two major categories within 2 min of testing time. Additionally, the sensor demonstrates the capability to distinguish between different types of tested fruits and vegetables, including garlic, green pepper, and nectarine. This rapid and minimally invasive sensing technology holds great promise for conducting field-based vegetable quality monitoring.
Why it matches plant phenotyping methods野菜のVOCを低侵襲に取得し、センサーアレイとスマートフォンで分類する測定プラットフォームの開発が研究の中心であり、野菜の品質・表現型分類に直接用いられている。
abstractHere, we developed a paper-based colorimetric sensor array combined with needles that could: 1) induce vegetable VOC release in a minimally invasive fashion, and 2) analyze VOCs in situ with a smartphone reader device.
Developing F3 transgenic segregants has significant potential to improve cayenne pepper varieties. However, current evaluation methods are often inconsistent and inaccurate, hindering the identification of effective traits. Traditional approaches only focus on a few aspects, thus not covering the full potential performance of the genotype. Utilizing morphometric image processing and categorical parameter assessment can fill the gap of traditional approaches to improving accuracy and objectivity in evaluation. In addition, environmental factors affecting the evaluation process are not adequately considered, making the results unreliable. Therefore, a systematic evaluation framework integrating morphometric analysis, categorical assessment, and environmental correction is essential for optimizing F3 cayenne transgressive segregants. The study aims to develop a synchronized assessment and selection approach based on agronomic, fruit morphometric, and categorical traits in evaluating F3 cayenne transgressive segregants. This research was designed with a randomized completed block design with 16 transgressive segregant genotypes and three check varieties. Each genotype was repeated three times, resulting in 57 experimental units. Based on the results of this study, quantitative and categorical indices could be used to selectively and systematically evaluate potential transgressive segregants in F3 cayenne peppers. The quantitative index is formed from outcome selection criteria, number of productive branches, area, and major axes weighted through an unbiased linear estimation approach, heritability, and best path analysis. Seven genotypes demonstrated superior transgressive performance based on quantitative indices, with G10.9.2, G10.7.1, and G6.8.5 excelling in both agronomic traits and categorical evaluations. These lines can be recommended for yield evaluation and hybrid cross-parents.
Why it matches plant phenotyping methodsトウガラシF3系統の評価・選抜を目的に、果実のモルフォメトリック画像処理を含む統合的な表現型評価フレームワークを開発しており、形質取得・評価手法が中心である。
abstractUtilizing morphometric image processing and categorical parameter assessment can fill the gap of traditional approaches to improving accuracy and objectivity in evaluation.
Chili pepper (Capsicum annuum L.), one of the most economically important vegetable crops globally, faces significant economic risks from anthracnose, leading to yield losses of 10% as well as decreasing marketability. Early and accurate detection is essential for mitigating these effects. Recent advancements in deep learning, particularly in image recognition, offer promising solutions for plant disease detection. This study applies deep learning models-MobileNet, ResNet50v2, and Xception-using transfer learning to diagnose anthracnose in chili peppers. A key challenge is the need for large, labeled datasets, which are costly to obtain. The study aims to identify the minimum dataset size required for accurate and efficient disease diagnosis using limited data. Performance metrics, including precision, recall, F1-score, and accuracy, were evaluated across different dataset sizes (500, 1,000, 2,000, 3,000, and 4,000 samples). Results indicated that model performance improves with larger datasets, with ResNet50v2 and Xception requiring more data to achieve optimal accuracy, while MobileNet showed strong generalization even with smaller datasets. These findings underscore the effectiveness of transfer learning-based models in plant disease detection, offering practical guidelines for balancing data availability and model performance in agricultural applications. Source code available at https://github.com/smart-able/Anthracnose.git.
Why it matches plant phenotyping methods唐辛子の病徴を画像から診断する深層学習モデルを比較し、データセット規模と性能の関係を評価しており、植物病害状態の取得・推定手法が中心である。
abstractThis study applies deep learning models-MobileNet, ResNet50v2, and Xception-using transfer learning to diagnose anthracnose in chili peppers.
Reproduction assets foundThe paper's abstract explicitly states that the authors' source code for the CNN-based anthracnose diagnosis analysis is publicly available on GitHub. No separate image dataset deposit by the authors is stated (images came from AIHub and their own collection), and supplementary materials are only hosted on the journal.Code · publicSource code available at https://github.com/smart-able/Anthracnose.git .Open asset ↗smart-able/Anthracnoselines:1-28Plant phenotyping relevance match · UnverifiedCrossref · Europe PMC · checked 6 Sept 2026
In agriculture, promptly and accurately identifying leaf diseases is crucial for sustainable crop production. To address this requirement, this research introduces a hybrid deep learning model that combines the visual geometric group version 19 (VGG19) architecture features with the transformer encoder blocks. This fusion enables the accurate and précised real-time classification of leaf diseases affecting grape, bell pepper, and tomato plants. Incorporating transformer encoder blocks offers enhanced capability in capturing intricate spatial dependencies within leaf images, promising agricultural sustainability and food security. By providing farmers and farming stakeholders with a reliable tool for rapid disease detection, our model facilitates timely intervention and management practices, ultimately leading to improved crop yields and mitigated economic losses. Through extensive comparative analyses on various datasets and filed tests, the proposed depth wise separable convolutional-TransNet (DSC-TransNet) architecture has demonstrated higher performance in terms of accuracy (99.97%), precision (99.94%), recall (99.94), sensitivity (99.94%), F1-score (99.94%), AUC (0.98) for Grpae leaves across different datasets including bell pepper and tomato. Furthermore, including DSC layers enhances the computational efficiency of the model while maintaining expressive power, making it well-suited for real-time agricultural applications. The developed DSC-TransNet model is deployed in NVIDIA Jetson Nano single board computer. This research contributes to advancing the field of automated plant disease classification, addressing critical challenges in modern agriculture and promoting more efficient and sustainable farming practices.
Why it matches plant phenotyping methods葉画像から植物病害を分類する深層学習モデルを開発し、複数データセットで比較評価するとともにエッジデバイスへ実装しており、植物病害状態の取得・推定手法が研究の中心である。
abstractthe proposed depth wise separable convolutional-TransNet (DSC-TransNet) architecture has demonstrated higher performance in terms of accuracy (99.97%), precision (99.94%), recall (99.94), sensitivity (99.94%), F1-score (99.94%), AUC (0.98)
Chili pepper (Capsicum spp.) fruits are used as vegetables, spices, and ornamental plants, necessitating various fruit characteristics. However, their genetic improvement is challenging through conventional crossbreeding due to the quantitative traits, which makes it difficult to predict phenotypes in the progeny. As a breakthrough, we focused on phenotypic simulation via genomic prediction (GP) and aimed to clarify its utility for fruit-related traits in chili peppers. The present study used 291 C. annuum accessions, including two populations: inbred lines and F 1 accessions derived from 20 inbred parents. We collected data of fruit length, width, shape index (length/width), weight, and pericarp thickness, and obtained single nucleotide polymorphism data via multiplexed inter-simple sequence repeat genotyping by sequencing. We simulated the fruit-related traits in the F 1 accessions by inputting their estimated genotypes (based on their parents) into the GP model using the GBLUP-GAUSS model, which was shown to be the most accurate regardless of population or trait differences in the present study. As a result, we observed strong positive correlations (r = 0.833-0.908) between the simulated and observed phenotypic values across all traits, suggesting that accurate ranking of F 1 progenies based on fruit-related traits can be achieved using parental information. This is the first report demonstrating the utility of phenotypic simulation via GP in chili pepper breeding, offering valuable insights for its application in this field.
Why it matches plant phenotyping methods親由来のゲノム情報から果実形質を予測する計算的フェノタイピング手法を開発・評価し、観測値との相関で精度を検証しているため、方法が中心的である。
abstractWe simulated the fruit-related traits in the F 1 accessions by inputting their estimated genotypes (based on their parents) into the GP model using the GBLUP-GAUSS model
Pepper production is a critical component of the global agricultural economy, with exports reaching a remarkable $6.9B in 2023. This underscores the crop’s importance as a major economic driver of export revenue for producing nations. Botrytis cinerea, the causative agent of gray mold, significantly impacts crops like fruits and vegetables, including peppers. Early detection of this pathogen is crucial for a reduction in fungicide reliance and economic loss prevention. Traditionally, visual inspection has been a primary method for detection. However, symptoms often appear after the pathogen has begun to spread. This study employs the Deep Learning algorithm YOLO for single-class segmentation on plant images to extract spatial details of pepper leaves. The dataset included hyperspectral images at discrete wavelengths (460 nm, 540 nm, 640 nm, 775 nm, and 875 nm) from derived vegetation indices (CVI, GNDVI, NDVI, NPCI, and PSRI) and from RGB. At an Intersection over Union with a 0.5 threshold, the Mean Average Precision (mAP50) achieved by the leaf-segmentation solution YOLOv11-Small was 86.4%. The extracted leaf segments were processed by multiple Transformer models, each yielding a descriptor. These descriptors were combined in ensemble and classified into three distinct classes using a K-nearest neighbor, a Long Short-Term Memory (LSTM), and a ResNet solution. The Transformer models that comprised the best ensemble classifier were as follows: the Swin-L (P:4 × 4–W:12 × 12), the ViT-L (P:16 × 16), the VOLO (D:5), and the XCIT-L (L:24–P:16 × 16), with the LSTM-based classification solution on the RGB, CVI, GNDVI, NDVI, and PSRI image sets. The classifier achieved an overall accuracy of 87.42% with an F1-Score of 81.13%. The per-class F1-Scores for the three classes were 85.25%, 66.67%, and 78.26%, respectively. Moreover, for B. cinerea detection during the initial as well as quiescent stages of infection prior to symptom development, qPCR-based methods (RT-qPCR) were used for quantification of in planta fungal biomass and integrated with the findings from the AI approach to offer a comprehensive strategy. The study demonstrates early and accurate detection of B. cinerea on pepper plants by combining segmentation techniques with Transformer model descriptors, ensembled for classification. This approach marks a significant step forward in the detection and management of crop diseases, highlighting the potential to integrate such methods into in situ systems like mobile apps or robots.
Why it matches plant phenotyping methods植物画像から葉領域を抽出し、病害状態を分類するAI画像解析手法が研究の中心であり、植物の病害表現型を直接推定しているため収載。
abstractThis study employs the Deep Learning algorithm YOLO for single-class segmentation on plant images to extract spatial details of pepper leaves.
Due to the small and irregular shapes of vegetable seeds, modeling them is challenging, and the imprecision of physical parameters hinders the performance of vegetable seeders, impeding simulation development. In this study, seeds of cucumber, pepper, and tomato were seen as examples. A 3D point cloud reconstruction method based on Structure-from-Motion Multi-View Stereo (SfM-MVS) was employed to accurately extract 3D models of small and irregularly shaped seeds. Corresponding discrete element models were established. Combining physical and simulation experiments on seed angle of repose(AOR), significant parameters influencing seed AOR and their ranges were identified through Plackett-Burman Design (PBD) and steepest ascent test. Within this range, the GA-BP-GA algorithm was used to accurately inverse the optimal parameter combination. The results indicate that the SfM-MVS 3D point cloud reconstruction method can extract more detailed shape information of small and irregularly shaped seeds. The GA-BP-GA algorithm achieved an inversion of physical parameters with the smallest relative error of cucumber, pepper, and tomato seeds being 0.26%, 0.98%, and 0.51%, respectively. Through experimental comparative analysis, the feasibility and accuracy of this method in calibrating discrete element parameters for small and irregularly shaped seeds were validated. The established seed models and calibrated parameters in this study can be implemented to the simulation optimization design of vegetable seeders, enhancing development efficiency and operational performance.
Why it matches plant phenotyping methodsSfM-MVSによる種子の3D形状取得と、形状情報を用いたDEMパラメータ校正が中心的な技術貢献であり、種子という植物器官の形態計測を扱っている。
abstractA 3D point cloud reconstruction method based on Structure-from-Motion Multi-View Stereo (SfM-MVS) was employed to accurately extract 3D models of small and irregularly shaped seeds.
Published1 Jan 2025Journal of food composition and analysis : an official publication of the United Nations University, International Network of Food Data Systems
Chilli is a widely produced crop, highly valued for its capsaicin content, a key economic trait. Traditional wet chemistry methods for estimating capsaicin are time-taking and laborious, while non-destructive methods like NIRS coupled with chemometrics, offer efficient alternatives, simplifying and accelerating biochemical assessments. This study is the first to develop and validate and tested for applicability of NIRS-based prediction model for capsaicin content in Indian chilli germplasm using MPLS regression. Various mathematical treatments were performed, and the most suited model was selected based on high RSQₑₓₜₑᵣₙₐₗ, RPD and lower SEP values in the external validation set, indicating strong prediction accuracy and minimal error. The model achieved high RSQₑₓₜₑᵣₙₐₗ value of 0.808, RPD value of 2.088 and low SEP value of 3.415 for capsaicin content, demonstrating excellent prediction performance. A paired sample t-test p-value of 0.757 (p > 0.05) showed non-significant difference between wet lab and predicted values, confirming the model’s accuracy. The applicability of the model was validated on fresh harvest germplasm the following year, showing a higher reliability score of 0.949, further confirming model’s reliability. This model would aid in high-throughput, accurate screening of chilli germplasm for capsaicin, accelerating chilli crop improvement programs and the development of new high-capsaicin varieties.
Why it matches plant phenotyping methods唐辛子果実のカプサイシン含量という植物形質をNIRSで非破壊推定する予測モデルを開発し、外部検証と翌年 germplasm での適用検証まで行っており、形質取得法が研究の中心である。
abstractThis study is the first to develop and validate and tested for applicability of NIRS-based prediction model for capsaicin content in Indian chilli germplasm using MPLS regression.
This study explores the effects of varying exposure times of microelement fertilization on hydrochemical parameters, plant growth, and nutrient content in an aquaponic system cultivating Capsicum annuum L. (pepper) with Cyprinus carpio ( Common carp L.). It also investigates the potential of visible-near-infrared (VIS-NIR) spectroscopy to differentiate between treated plants based on their spectral characteristics. The findings aim to enhance the understanding of microelement dynamics in aquaponics and optimize the use of VIS-NIR spectroscopy for nutrient and stress detection in crops. The effects of microelement exposure on the growth and health of Cyprinus carpio ( Common carp L.) in an aquaponic system are investigated, demonstrating a 100% survival rate and optimal growth performance. The findings suggest that microelement treatments, when applied within safe limits, can enhance system productivity without compromising fish health. Concerning hydrochemical parameters, conductivity remained stable, with values ranging from 271.66 to 297.66 μS/cm, while pH and dissolved oxygen levels were within optimal ranges for aquaponic systems. Ammonia nitrogen levels decreased significantly in treated variants, suggesting improved water quality, while nitrate and orthophosphate reductions indicated an enhanced plant nutrient uptake. The findings underscore the importance of managing water chemistry to maintain a balanced and productive aquaponic system. The increase in root length observed in treatments 2 and 6 suggests that certain microelement exposure times may enhance root development, with treatment 6 showing the longest roots (58.33 cm). Despite this, treatment 2 had a lower biomass (61.2 g), indicating that root growth did not necessarily translate into increased plant weight, possibly due to energy being directed towards root development over fruit production. In contrast, treatment 6 showed both the greatest root length and the highest weight (133.4 g), suggesting a positive correlation between root development and fruit biomass. Yield data revealed that treatment 4 produced the highest yield (0.144 g), suggesting an optimal exposure time before nutrient imbalances negatively impact growth. These results highlight the complexity of microelement exposure in aquaponic systems, emphasizing the importance of fine-tuning exposure times to balance root growth, biomass, and yield for optimal plant development. The spectral characteristics of the visible-near-infrared region of pepper plants treated with microelements revealed subtle differences, particularly in the green (534-555 nm) and red edge (680-750 nm) regions. SIMCA models successfully classified control and treated plants with a misclassification rate of only 1.6%, highlighting the effectiveness of the spectral data for plant differentiation. Key wavelengths for distinguishing plant classes were 468 nm, 537 nm, 687 nm, 728 nm, and 969 nm, which were closely related to plant pigment content and nutrient status. These findings suggest that spectral analysis can be a valuable tool for the non-destructive assessment of plant health and nutrient status.
Why it matches plant phenotyping methodsVIS-NIR分光とSIMCA分類を用いて、処理植物の識別および健康・栄養状態を非破壊評価する方法が、研究の明示的な主要目的として扱われているため。
abstractIt also investigates the potential of visible-near-infrared (VIS-NIR) spectroscopy to differentiate between treated plants based on their spectral characteristics.
Pepper / chilliGreenhouseLaboratory / benchtopNeRF / 3D Gaussian SplattingLiDAR / point cloudFruitMorphology / geometry measurement2D/3D reconstructionImage / point-cloud registrationGrowth / development / phenology
Accurate collection of plant phenotyping is critical to optimising sustainable farming practices in precision agriculture. Traditional phenotyping in controlled laboratory environments, while valuable, falls short in understanding plant growth under real-world conditions. Emerging sensor and digital technologies offer a promising approach for direct phenotyping of plants in farm environments. This study investigates a learning-based phenotyping method using neural implicit surfaces reconstruction to achieve accurate in situ phenotyping of pepper plants in greenhouse environments. To quantitatively evaluate the performance of this method, traditional point cloud registration on 3D scanning data is implemented for comparison. Experimental result shows that NIR (neural implicit surfaces reconstruction) achieves competitive accuracy compared to the 3D scanning method. The mean distance error between the scanner-based method and the NeRF (neural radiance fields)-based method is 0.811 mm. This study shows that the learning-based NeRF method has similar accuracy to the 3D scanning-based method but with greater scalability and faster deployment capabilities.
Why it matches plant phenotyping methods植物の3D表現型を取得するNeRFベース手法を開発し、3Dスキャン法と精度比較・検証しており、表現型取得法が研究の中心である。
abstractThis study investigates a learning-based phenotyping method using neural implicit surfaces reconstruction to achieve accurate in situ phenotyping of pepper plants in greenhouse environments.
This study investigates the sensory qualities, weight loss and texture changes in green bell peppers as indicator of freshness during storage. It assesses the feasibility of using machine learning methods to monitor freshness changes in the commercial variety ‘ Kyohikari ’ over a 16-d storage period at + 5 °C and 95 % relative humidity (RH). Throughout the storage period, the commercial variety of green bell peppers were stored, and RGB images were captured using a DSLR camera. Sensory assessments and measurements of texture, weight loss, chlorophyll, and carotenoids were conducted at various inteval. Several machine learning approaches- including logistic regression, neural networks, random forests, k-nearest neighbors, and support vector machines, were employed to develop classification and prediction models for fruit freshness during storage intervals of 0, 4, 6, 8, 10, 12, 14, and 16 d. The results indicate that hue angle and chlorophyll content remained unchanged throughout the experiment. However, after 10 d of storage, a 3 % weight loss was observed, accompanied by the detection of off-odors and off-flavors, marking the limit of marketability. The models demonstrated exceptional accuracy in classifying and predicting the freshness of green bell peppers on the storage day, achieving 100 % accuracy with the neural network algorithm. • Green bell peppers from 5 different orchards lost freshness by storage at + 5 ºC. • Machine Learning was used to classify and predict fruit freshness by storage days. • Four methods were applied to build the classification and prediction models. • Neural Network showed 100 % accuracy for classification and prediction models.
Why it matches plant phenotyping methodsRGB画像と機械学習によってピーマン果実の保存中の鮮度状態を分類・予測する手法が研究の中心であり、植物器官の状態推定に該当する。
titleUtilizing RGB imaging and machine learning for freshness level determination of green bell pepper (Capsicum annuum L.) throughout its shelf-life
Due to the vulnerability of pepper ( Capsicum spp.) and the virulence of tomato spotted wilt virus (TSWV), seasonal shortages and surges of prices are a challenge and thus threaten household income. Traditional bioassays for detecting TSWV, such as observation for symptoms and reverse transcription-PCR, are time-consuming, labor-intensive, and sometimes lack precision, highlighting the need for a faster and more reliable approach to plant disease assessment. Here, two imaging techniques-Red-Green-Blue (RGB) and hyperspectral imaging (using NDVI and wavelength intensities)-were compared with a bioassay method to study the incidence and severity of TSWV in different pepper accessions. The bioassay results gave TSWV an incidence from 0 to 100% among the accessions, while severity ranged from 0 to 5.68% based on RGB analysis. The normalized difference vegetative index (NDVI) scored from 0.21 to 0.23 for healthy spots on the leaf but from 0.14 to 0.19 for disease spots, depending on the severity of the damage. The peak reflectance of the disease spots on the leaves was identified in the visible light spectrum (430-470 nm) when spectral bands were studied in the broad spectrum (400.93-1004.5 nm). For the selected wavelength in the visible light spectrum, a high reflectance intensity of 340 to 430 was identified for disease areas, but between 270 and 290 for healthy leaves. RGB and hyperspectral imaging techniques can be recommended for precise and accurate detection and quantification of TSWV infection.
Why it matches plant phenotyping methodsRGBおよびハイパースペクトル画像で植物病害の発生・重症度を検出・定量し、バイオアッセイと比較評価することが研究の中心であるため、植物フェノタイピング手法研究として含める。
abstractHere, two imaging techniques-Red-Green-Blue (RGB) and hyperspectral imaging (using NDVI and wavelength intensities)-were compared with a bioassay method to study the incidence and severity of TSWV in different pepper accessions.
The phenomenon of fluctuating chili prices can be resolved in stages, one of which is through multiple crosses. However, this cross requires precise methods in the evaluation and selection process, especially regarding fruit characteristics. Image-based phenotyping 4.0 approaches can increase the potential precision of such evaluation genotypes, especially when this approach is combined with multivariate analysis. Therefore, both methods are needed to evaluate and select these cayenne multiple crosses. This research aims to identify the effectiveness of multivariate analysis and image-based explanatory characteristics of fruit phenotypes and to select multiple crosses that can continue to the F2 generation. The research was designed with a randomized complete block design of ten F1 multiple cross-genotypes and four check varieties. Each genotype was repeated three times, so there were 42 experimental units. Based on the results, multivariate was considered adequate in determining image explanatory characters based on fruit phenotype and genotype mapping of the population diversity of multiple crosses of cayenne pepper. The characteristics of fruit height, fruit area, and fruit Intden are image-based explanatory characters that can map the completeness of cayenne pepper fruit between multiple crosses well. This indicates that image-based phenotyping and multivariate analysis can provide more detailed image information of the potential of cayenne fruit from multiple crosses than just based on fruit weight. Therefore, both approaches are recommended for analyzing cayenne paper fruit potential, especially for multiple crosses. In addition, three crosses (MC4, MC8, and MC9) are optimal for the next generation to be recommended and continued.
Why it matches plant phenotyping methodsトウガラシ果実形質の画像ベース表現型解析と多変量解析が、交雑系統の評価・選抜の中心的手法として扱われているため。
abstractImage-based phenotyping 4.0 approaches can increase the potential precision of such evaluation genotypes, especially when this approach is combined with multivariate analysis.
A new deep-learning model for classifying and detecting plant diseases in chilli plants is described. It is built on a modified version of the MobileNet architecture. The model overcomes conventional diagnostic tools’ high computing costs and restricted adaptability by combining sophisticated optimisation models and reliable training procedures. The model considerably reduces the time and resources needed for an accurate diagnosis while effectively managing complicated illness presentations, with a diagnostic accuracy of 97.18%. Using the chilli leaf picture dataset, data augmentation, and finetuning techniques, the model shows promise for real-time disease diagnosis in agricultural environments. The study underscores the importance of high-quality image data and extensive training datasets, calling for further evaluation across various climatic and environmental conditions to ensure robustness and adaptability. This research opens new opportunities for AI-based models in diverse agricultural contexts, potentially leading to significant advancements in precision farming.
Why it matches plant phenotyping methods唐辛子葉画像から植物病害を検出・分類する深層学習モデルの開発が研究の中心であり、植物の病害状態を直接推定するため、植物フェノタイピング手法に該当する。
abstractA new deep-learning model for classifying and detecting plant diseases in chilli plants is described.
The agricultural sector faces significant losses due to plant diseases, particularly in major crops such as potatoes, tomatoes, and bell peppers. This paper presents a machine learning-based approach to classify diseases in these crops using leaf images. A Convolutional Neural Network (CNN) model was constructed and trained on datasets of healthy leaf images and diseased leaf images from potato, tomato, and bell pepper plants. The model successfully classifies diseases such as Bacterial Spot (for bell peppers), Early Blight, Late Blight, Mosaic Virus, Leaf Mold (for tomatoes), and with a classification accuracy of 93%, this system provides early detection, helping farmers take timely action to reduce disease impact and increase crop yield.
Why it matches plant phenotyping methods葉画像から植物病害を分類するCNN手法が研究の中心であり、植物の病害状態を直接推定するため、植物フェノタイピング手法として採用する。
abstractThis paper presents a machine learning-based approach to classify diseases in these crops using leaf images.
This article aims to develop a novel Artificial Intelligence-powered Internet of Things (AI-powered IoT) system that can automatically monitor the conditions of the plant (crop) and apply the necessary action without human interaction. The system can remotely send a report on the plant conditions to the farmers through IoT, enabling them for tracking the healthiness of plants. Chili plant has been selected to test the proposed AI-powered IoT monitoring and actuating system as it is so sensitive to the soil moisture, weather changes and can be attacked by several types of diseases. The structure of the proposed system is passed through five main stages, namely, AI-powered IoT system design, prototype fabrication, signal and image processing, noise elimination and proposed system testing. The prototype for monitoring is equipped with multiple sensors, namely, soil moisture, carbon dioxide (CO 2 ) detector, temperature, and camera sensors, which are utilized to continuously monitor the conditions of the plant. Several signal and image processing operations have been applied on the acquired sensors data to prepare them for further post-processing stage. In the post processing step, a new AI based noise elimination algorithm has been introduced to eliminate the noise in the images and take the right actions which are performed using actuators such as pumps, fans to make the necessary actions. The experimental results show that the prototype is functioning well with the proposed AI-powered IoT algorithm, where the water pump, exhausted fan and pesticide pump are actuated when the sensors detect a low moisture level, high CO 2 concentration level, and video processing-based pests' detection, respectively. The results also show that the algorithm is capable to detect the pests on the leaves with 75% successful rate.
Why it matches plant phenotyping methods植物状態を監視するAI・IoTシステムを設計し、画像処理とノイズ除去によって葉上の害虫を検出する手法を中心的に開発・試験しているため、植物状態の計測プラットフォームとして収録する。
abstractThis article aims to develop a novel Artificial Intelligence-powered Internet of Things (AI-powered IoT) system that can automatically monitor the conditions of the plant (crop)
Leaf disease detection is critical in agriculture, as it directly impacts crop health, yield, and quality. Early and accurate detection of leaf diseases can prevent the spread of infections, reduce the need for chemical treatments, and minimize crop losses. This not only ensures food security but also supports sustainable farming practices. Effective leaf disease detection systems empower farmers with the knowledge to take timely actions, leading to healthier crops and more efficient resource management. In an era of increasing global food demand and environmental challenges, advanced leaf disease detection technologies are indispensable for modern agriculture. This study presents an innovative approach for detecting pepper bell leaf disease using an ANFIS Fuzzy convolutional neural network (CNN) integrated with local binary pattern (LBP) features. Experiments involve using the models without LBP, as well as, with LBP features. For both sets of experiments, the proposed ANFIS CNN model performs superbly. It shows an accuracy score of 0.8478 without using LBP features while its precision, recall, and F1 scores are 0.8959, 0.9045, and 0.8953, respectively. Incorporating LBP features, the proposed model achieved exceptional performance, with accuracy, precision, recall, and an F1 score of higher than 99%. Comprehensive comparisons with state-of-the-art techniques further highlight the superiority of the proposed method. Additionally, cross-validation was applied to ensure the robustness and reliability of the results. This approach demonstrates a significant advancement in agricultural disease detection, promising enhanced accuracy and efficiency in real-world applications.
Why it matches plant phenotyping methods画像からコショウ葉の病害状態を推定するCNN手法を開発・比較・検証しており、植物病害フェノタイピング手法が中心です。
abstractThis study presents an innovative approach for detecting pepper bell leaf disease using an ANFIS Fuzzy convolutional neural network (CNN) integrated with local binary pattern (LBP) features.
Reproduction assets foundThe paper's leaf disease detection experiments use public Kaggle leaf image datasets. The methods section cites the PlantVillage pepper bell dataset (2,475 images) with a direct access link, and the data availability statement points to the New Plant Diseases Dataset. Both are public, paper-specific image assets used直接Dataset · publicom Forest; HoG, Histogram of Oriented Gradients; SVM, Support Vector Machine; ETC, Extra tree classifier; ANFIS, Adaptive neuro-fuzzy inference system; VRAM, Video random access memory; GPU, General processing unit.
Data availability statement
Publicly available datasets were analyzed in this study. This data can be found here: https://www.kaggle.com/datasets/vipoooool/new-plant-diseases-dataset .
Author contributions
T-hK: Conceptualization, Data curation, Writing – original draft. MS: Conceptualization, Formal Analysis, Writing – original draft. BA: Funding acquisition, Methodology, Writing – original draft. NI: Investigation, Project administration, Writing – original draft. JB: ResourcesOpen asset ↗Kaggle · vipoooool/new-plant-diseases-datasetlines:588-605Plant phenotyping relevance match · UnverifiedEurope PMC · checked 15 Sept 2026
With the recent development of smart farms, researchers are very interested in such fields. In particular, the field of disease diagnosis is the most important factor. Disease diagnosis belongs to the field of anomaly detection and aims to distinguish whether plants or fruits are normal or abnormal. The problem can be solved by binary or multi-classification based on a Convolutional Neural Network (CNN), but it can also be solved by image reconstruction. However, due to the limitation of the performance of image generation, SOTA's methods propose a score calculation method using a latent vector error. In this paper, we propose a network that focuses on chili peppers and proceeds with background removal through GrabCut. It shows a high performance through an image-based score calculation method. Due to the difficulty of reconstructing the input image, the difference between the input and output images is large. However, the serial autoencoder proposed in this paper uses the difference between the two fake images, instead of the actual input, as a score. We propose a method of generating meaningful images using the GAN structure and classifying three results simultaneously by one discriminator. The proposed method showed a higher performance than previous research, and image-based scores showed the best performance.
Why it matches plant phenotyping methodsチリ pepper の病害状態を画像から診断する手法を開発し、背景除去、画像再構成、スコア計算、分類性能を中心に評価しているため、植物表現型(病害状態)の取得・推定方法が中心である。
abstractIn this paper, we propose a network that focuses on chili peppers and proceeds with background removal through GrabCut.
This research explores a new method for assessing chili plant health using multispectral camera imagery and deep learning-based image segmentation. Data from Bale Tatanen Universitas Padjadjaran chili farms were used to create a labeled dataset with four health categories. Initial image processing involved FastSAM inference to generate binary masks, followed by training a YOLOv8 model for improved segmentation accuracy. This model enabled NDVI calculation and automatic health labeling in unseen images, contributing to automated chili plant health monitoring systems. Evaluation showed an average Dice Coefficient of 63.80%, indicating moderate overlap between predicted and true masks. High precision and robust segmentation across various conditions were observed, though improvements are needed in fine detail segmentation. This approach enhances understanding of chili plant health and supports further studies in the field.
Why it matches plant phenotyping methodsマルチスペクトル画像のセグメンテーション、ラベル付きデータセット構築、未見画像での健康状態自動推定を中心とする植物フェノタイピング手法研究であり、性能評価も実施している。
abstractThis research explores a new method for assessing chili plant health using multispectral camera imagery and deep learning-based image segmentation.
Background The automation of pest monitoring is highly important for enhancing integrated pest management in practice. In this context, advanced technologies are becoming increasingly explored. Hyperspectral imaging (HSI) is a technique that has been used frequently in recent years in the context of natural science, and the successful detection of several fungal diseases and some pests has been reported. Various automated measures and image analysis methods offer great potential for enhancing monitoring in practice. Results In this study, the use of hyperspectral imaging over a wide spectrum from 400 to 2500 nm is investigated for noninvasive identification and the distinction of healthy plants and plants infested with Myzus persicae (Sulzer) and Frankliniella occidentalis (Pergande) on bell peppers. Pest infestations were carried out in netted areas, and images of single plants and dissected leaves were used to train the decision algorithm. Additionally, a specially modified spraying robot was converted into an autonomous platform used to carry the hyperspectral imaging system to take images under greenhouse conditions. The algorithm was developed via the XGBoost framework with gradient-boosted trees. Signals from specific wavelengths were found to be associated with the damage patterns of different insects. Under confined conditions, M. persicae and F. occidentalis infestations were distinguished from each other and from the uninfested control for single leaves. Differentiation was still possible when small whole plants were used. However, application under greenhouse conditions did not result in a good fit compared to the results of manual monitoring. Conclusion Hyperspectral images can be used to distinguish sucking pests on bell peppers on the basis of single leaves and intact potted bell pepper plants under controlled conditions. Wavelength reduction methods offer options for multispectral camera usage in high-grown vegetable greenhouses. The application of automated platforms similar to the one tested in this study could be possible, but for successful pest detection under greenhouse conditions, algorithms should be further developed fully considering real-world conditions.
Why it matches plant phenotyping methodsベルペッパー植物の害虫による状態・損傷パターンをハイパースペクトル画像と機械学習で識別する手法を開発・検証し、自律撮像プラットフォームも評価しているため、植物フェノタイピング手法が中心である。
abstractthe use of hyperspectral imaging over a wide spectrum from 400 to 2500 nm is investigated for noninvasive identification and the distinction of healthy plants and plants infested with Myzus persicae (Sulzer) and Frankliniella occidentalis (Pergande) on bell peppers.
Abstract: Preserving plant health and early detection of diseases are crucial in modern agriculture. Artificial intelligence techniques, particularly deep learning networks, are employed for this purpose. In this study, disease recognition was conducted using leaf images from various plant species. The study encompassed important agricultural products such as apples, strawberries, grapes, corn, peppers, and potatoes among the plant species considered. Among the deep learning networks, popular architectures like AlexNet, Vgg16, MobileNetV2, and Inception were compared. The Inception V3 model achieved the highest success rate of 92%, followed by the AlexNet architecture with a success rate of 91%. Among these networks, the InceptionV3 model yielded the best results. The InceptionV3 model effectively learned from plant leaf images and accurately distinguished between diseased and healthy leaves. These findings demonstrate that AI-based systems can be efficiently utilized for disease recognition and prevention in the agriculture sector. In this study, the performance of the InceptionV3 model in disease recognition on plant leaves was analyzed in detail, emphasizing the role of deep learning networks in agricultural applications.
Why it matches plant phenotyping methods植物葉画像から健全・罹病状態を推定する深層学習手法を比較・評価しており、植物病害表現型の取得・分類が研究の中心です。
abstractIn this study, disease recognition was conducted using leaf images from various plant species.
Reproduction assets foundThe paper's plant-disease classification experiments were performed on the public New Plant Diseases Dataset (Kaggle), which the authors explicitly state is openly accessible via a Kaggle URL. This is a paper-specific, public, actionable phenotype image dataset. No author analysis code or trained models are reported asDataset · publicsector. Suggestions for
future research include the use of larger and more
diverse datasets and the application of federated learning
techniques, which can improve the performance of the
model and provide security.
Dataset Access: The dataset used in this study is open
and can be accessed from the relevant source link.
Access:
https://www.kaggle.com/datasets/vipoooool/new-plant-diseases-dataset.Open asset ↗Kaggle · vipoooool/new-plant-diseases-datasetpdf-raw-page:11 lines:97-114Code / dataset availability confirmedOpenAlex · arXiv · checked 7 Sept 2026
In modern agriculture, precise monitoring of plants and fruits is crucial for tasks such as high-throughput phenotyping and automated harvesting. This paper addresses the challenge of reconstructing accurate 3D shapes of fruits from partial views, which is common in agricultural settings. We introduce CF-PRNet, a coarse-to-fine prototype refining network, leverages high-resolution 3D data during the training phase but requires only a single RGB-D image for real-time inference. Our approach begins by extracting the incomplete point cloud data that constructed from a partial view of a fruit with a series of convolutional blocks. The extracted features inform the generation of scaling vectors that refine two sequentially constructed 3D mesh prototypes - one coarse and one fine-grained. This progressive refinement facilitates the detailed completion of the final point clouds, achieving detailed and accurate reconstructions. CF-PRNet demonstrates excellent performance metrics with a Chamfer Distance of 3.78, an F1 Score of 66.76%, a Precision of 56.56%, and a Recall of 85.31%, and win the first place in the Shape Completion and Reconstruction of Sweet Peppers Challenge.
Why it matches plant phenotyping methods果実の部分RGB-D画像から3D形状を再構成する手法を開発・評価しており、植物器官の形態形質取得が研究の中心である。
abstractThis paper addresses the challenge of reconstructing accurate 3D shapes of fruits from partial views
Reproduction assets foundThe paper's authors publicly release their CF-PRNet source code for sweet pepper point cloud completion. The sweet pepper benchmark dataset is cited prior work (ref [2]), not a paper-specific asset, and the challenge website is a generic event page.Code · publicOur source code is
available at https://github.com/uqzhichen/CF-PRNet/.Open asset ↗uqzhichen/CF-PRNetpdf-page:1 lines:1-50Code / dataset availability confirmedarXiv · OpenAlex · checked 13 Sept 2026
Creation of new annotated public datasets is crucial in helping advances in 3D computer vision and machine learning meet their full potential for automatic interpretation of 3D plant models. Despite the proliferation of deep neural network architectures for segmentation and phenotyping of 3D plant models in the last decade, the amount of data, and diversity in terms of species and data acquisition modalities are far from sufficient for evaluation of such tools for their generalization ability. To contribute to closing this gap, we introduce PLANesT-3D; a new annotated dataset of 3D color point clouds of plants. PLANesT-3D is composed of 34 point cloud models representing 34 real plants from three different plant species: \textit{Capsicum annuum}, \textit{Rosa kordana}, and \textit{Ribes rubrum}. Both semantic labels in terms of "leaf" and "stem", and organ instance labels were manually annotated for the full point clouds. PLANesT-3D introduces diversity to existing datasets by adding point clouds of two new species and providing 3D data acquired with the low-cost SfM/MVS technique as opposed to laser scanning or expensive setups. Point clouds reconstructed with SfM/MVS modality exhibit challenges such as missing data, variable density, and illumination variations. As an additional contribution, SP-LSCnet, a novel semantic segmentation method that is a combination of unsupervised superpoint extraction and a 3D point-based deep learning approach is introduced and evaluated on the new dataset. The advantages of SP-LSCnet over other deep learning methods are its modular structure and increased interpretability. Two existing deep neural network architectures, PointNet++ and RoseSegNet, were also tested on the point clouds of PLANesT-3D for semantic segmentation.
Why it matches plant phenotyping methods3D植物点群の注釈付きデータセットを構築し、植物器官のセマンティック・インスタンス分割手法を開発・評価しており、植物フェノタイピング手法が中心である。
abstractwe introduce PLANesT-3D; a new annotated dataset of 3D color point clouds of plants.
Reproduction assets foundThe paper introduces PLANesT-3D, an annotated 3D plant point cloud dataset, and SP-LSCnet segmentation code, both explicitly stated as publicly available at the authors' Aperta record and GitHub repository.Dataset · publicThe PLANesT-3D dataset is publicly available at https://aperta.ulakbim.gov.tr/record/286354 and https://github.com/visionlab-ogu/PLANesT-3D/tree/main/dataOpen asset ↗aperta.ulakbim.gov.tr · 286354lines:83-145Dataset · publicThe 2D color images for all the 34 plants together with their estimated camera poses and parameters are also open to the public to provide input data for recent 3D reconstruction techniques 3 3
3
The data is available at https://github.com/visionlab-ogu/PLANesT-3D/tree/main/data .Open asset ↗github.com/visionlab-ogu/PLANesT-3Dlines:494-505Code · publicThe code for SP-LSCnet is available at https://github.com/visionlab-ogu/PLANesT-3DOpen asset ↗github.com/visionlab-ogu/PLANesT-3Dlines:146-154Plant phenotyping relevance match · UnverifiedEurope PMC · checked 7 Sept 2026
Background Pepper Phytophthora blight is a devastating disease during the growth process of peppers, significantly affecting their yield and quality. Accurate, rapid, and non-destructive early detection of pepper Phytophthora blight is of great importance for pepper production management. This study investigated the possibility of using multispectral imaging combined with machine learning to detect Phytophthora blight in peppers. Peppers were divided into two groups: one group was inoculated with Phytophthora blight, and the other was left untreated as a control. Multispectral images were collected at 0-h samples before inoculation and at 48, 60, 72, and 84 h after inoculation. The supporting software of the multispectral imaging system was used to extract spectral features from 19 wavelengths, and textural features were extracted using a gray-level co-occurrence matrix (GLCM) and a local binary pattern (LBP). The principal component analysis (PCA), successive projection algorithm (SPA), and genetic algorithm (GA) were used for feature selection from the extracted spectral and textural features. Two classification models were established based on effective single spectral features and significant spectral textural fusion features: a partial least squares discriminant analysis (PLS_DA) and one-dimensional convolutional neural network (1D-CNN). A two-dimensional convolutional neural network (2D-CNN) was constructed based on five principal component (PC) coefficients extracted from the spectral data using PCA, weighted, and summed with 19-channel multispectral images to create new PC images. Results The results indicated that the models using PCA for feature selection exhibit relatively stable classification performance. The accuracy of PLS-DA and 1D-CNN based on single spectral features is 82.6% and 83.3%, respectively, at the 48h mark. In contrast, the accuracy of PLS-DA and 1D-CNN based on spectral texture fusion reached 85.9% and 91.3%, respectively, at the same 48h mark. The accuracy of the 2D-CNN based on 5 PC images is 82%. Conclusions The research indicates that Phytophthora blight infection can be detected 48 h after inoculation (36 h before visible symptoms). This study provides an effective method for the early detection of Phytophthora blight in peppers.
Why it matches plant phenotyping methodsマルチスペクトル画像からコショウの病害状態を早期推定する画像解析・CNN手法が研究の中心であり、技術性能も評価しているため。
abstractThis study investigated the possibility of using multispectral imaging combined with machine learning to detect Phytophthora blight in peppers.
Plant height is an important parameter of plant phenotype as one indicator of plant growth. In view of the complexity and scale limitation in current measurement systems, a scaleless method is proposed for the automatic measurement of plant height based on monocular computer vision. In this study, four peppers planted side by side were used as the measurement objects. Two color images of the measurement object were obtained by using a monocular camera at different shooting heights. Binary images were obtained as the images were processed by super-green grayscale and the Otsu method. The binarized images were transformed into horizontal one-dimensional data by the statistical number of vertical pixels, and the boundary points of multiple plants in the image were found and segmented into single-plant binarized images by filtering and searching for valleys. The pixel height was extracted from the segmented single plant image and the pixel displacement of the height was calculated, which was substituted into the calculation together with the reference height displacement to obtain the realistic height of the plant and complete the height measurements of multiple plants. Within the range of 2–3 m, under the light condition of 279 lx and 324 lx, this method can realize the rapid detection of multi-plant phenotypic parameters with a high precision and obtain more accurate plant height measurement results. The absolute error of plant height measurement is not more than ±10 mm, and the absolute proportion error is not more than ±4%.
Why it matches plant phenotyping methods植物高を対象とした単眼コンピュータビジョンによる自動フェノタイピング手法の開発と精度評価が研究の中心である。
abstracta scaleless method is proposed for the automatic measurement of plant height based on monocular computer vision.
As the world population is expected to reach 10 billion by 2050, our agricultural production system needs to double its productivity despite a decline of human workforce in the agricultural sector. Autonomous robotic systems are one promising pathway to increase productivity by taking over labor-intensive manual tasks like fruit picking. To be effective, such systems need to monitor and interact with plants and fruits precisely, which is challenging due to the cluttered nature of agricultural environments causing, for example, strong occlusions. Thus, being able to estimate the complete 3D shapes of objects in presence of occlusions is crucial for automating operations such as fruit harvesting. In this paper, we propose the first publicly available 3D shape completion dataset for agricultural vision systems. We provide an RGB-D dataset for estimating the 3D shape of fruits. Specifically, our dataset contains RGB-D frames of single sweet peppers in lab conditions but also in a commercial greenhouse. For each fruit, we additionally collected high-precision point clouds that we use as ground truth. For acquiring the ground truth shape, we developed a measuring process that allows us to record data of real sweet pepper plants, both in the lab and in the greenhouse with high precision, and determine the shape of the sensed fruits. We release our dataset, consisting of almost 7,000 RGB-D frames belonging to more than 100 different fruits. We provide segmented RGB-D frames, with camera intrinsics to easily obtain colored point clouds, together with the corresponding high-precision, occlusion-free point clouds obtained with a high-precision laser scanner. We additionally enable evaluation of shape completion approaches on a hidden test set through a public challenge on a benchmark server.
Why it matches plant phenotyping methods果実の3D形状という植物器官の形態形質を対象に、RGB-D画像・高精度点群・評価用ベンチマークを構築しており、形状取得と推定の方法論が中心である。
abstractWe provide an RGB-D dataset for estimating the 3D shape of fruits.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Code · publicOur development toolkit including a data loader is available at:
https://github.com/PRBonn/shape_completion_toolkit for handling the dataset and computing metrics.Open asset ↗PRBonn/shape_completion_toolkitlines:55-81Plant phenotyping relevance match · UnverifiedEurope PMC · checked 7 Sept 2026
The use of low-cost depth imaging sensors is investigated to automate plant pathology tests. Spatial evolution is explored to discriminate plant resistance through the hypersensitive reaction involving cotyledon loss. A high temporal frame rate and a protocol operating with batches of plants enable to compensate for the low spatial resolution of depth cameras. Despite the high density of plants, a spatial drop of the depth is observed when the cotyledon loss occurs. We introduce a small and simple spatiotemporal feature space which is shown to carry enough information to automate the discrimination between batches of resistant (loss of cotyledons) and susceptible plants (no loss of cotyledons) with 97% accuracy and with a timing 30 times faster than for human annotation. The robustness of the method-in terms of density of plants in the batch and possible internal batch desynchronization-is assessed successfully with hundreds of varieties of Pepper in various environments. A study on the generalizability of the method suggests that it can be extended to other pathosystems and also to segregating plants, i.e., intermediate state with batches composed of resistant and susceptible plants. The imaging system developed, combined with the feature extraction method and classification model, provides a full pipeline with unequaled throughput and cost efficiency by comparison with the state-of-the-art one. This system can be deployed as a decision-support tool but is also compatible with a standalone technology where computation is done at the edge in real time.
Why it matches plant phenotyping methods低コスト深度画像による植物病害表現型(子葉脱落・抵抗性)の自動抽出、分類パイプラインを開発し、多数品種・環境で性能と頑健性を検証しており、表現型取得手法が中心である。
abstractThe use of low-cost depth imaging sensors is investigated to automate plant pathology tests.
Introduction Green pepper yield estimation is crucial for establishing harvest and storage strategies. Method This paper proposes an automatic counting method for green pepper fruits based on object detection and multi-object tracking algorithm. Green pepper fruits have colors similar to leaves and are often occluded by each other, posing challenges for detection. Based on the YOLOv5s, the CS_YOLOv5s model is specifically designed for green pepper fruit detection. In the CS_YOLOv5s model, a Slim-Nick combined with GSConv structure is utilized in the Neck to reduce model parameters while enhancing detection speed. Additionally, the CBAM attention mechanism is integrated into the Neck to enhance the feature perception of green peppers at various locations and enhance the feature extraction capabilities of the model. Result According to the test results, the CS_YOLOv5s model of mAP, Precision and Recall, and Detection time of a single image are 98.96%, 95%, 97.3%, and 6.3 ms respectively. Compared to the YOLOv5s model, the Detection time of a single image is reduced by 34.4%, while Recall and mAP values are improved. Additionally, for green pepper fruit tracking, this paper combines appearance matching algorithms and track optimization algorithms from SportsTrack to optimize the DeepSort algorithm. Considering three different scenarios of tracking, the MOTA and MOTP are stable, but the ID switch is reduced by 29.41%. Based on the CS_YOLOv5s model, the counting performance before and after DeepSort optimization is compared. For green pepper counting in videos, the optimized DeepSort algorithm achieves ACP (Average Counting Precision), MAE (Mean Absolute Error), and RMSE (Root Mean Squared Error) values of 95.33%, 3.33, and 3.74, respectively. Compared to the original algorithm, ACP increases by 7.2%, while MAE and RMSE decrease by 6.67 and 6.94, respectively. Additionally, Based on the optimized DeepSort, the fruit counting results using YOLOv5s model and CS_YOLOv5s model were compared, and the results show that using the better object detector CS_YOLOv5s has better counting accuracy and robustness.
Why it matches plant phenotyping methods緑 pepper果実の検出・追跡・計数という植物器官形質の画像ベース取得法を開発し、精度と追跡性能を検証しているため、中心的なフェノタイピング手法研究である。
abstractThis paper proposes an automatic counting method for green pepper fruits based on object detection and multi-object tracking algorithm.
Abstract The most significant peril to food safety arises from plant diseases, capable of substantially diminishing both the quantity and quality of agricultural yields. Identifying these plant diseases stands out as the foremost challenge within the agricultural sector. Convolutional and deep neural networks prove effective in resolving image classification challenges within the realm of computer vision. Numerous Deep Neural Network(DNN)-based structures have been employed to diagnose plant diseases. Many DNN models in the field make use of various iterations of Dense and DenseNet layers in order to enhance the receptive field and capture intricate features within the data. However, it is important to note that such models often come with a significant computational burden and can introduce aliasing artifacts due to their complexity and resource-intensive nature. To overcome those limitations, we proposed a novel Residual Nested Dilated DenseNet based deep-learning (RNDDNet) model in this paper. Residual Nested Dilated DenseNet model residual connections are achieving the required receptive field, and their dilation factors are effective in extracting more features. The RNDDNet model exhibits the highest level of accuracy in identifying plant diseases. This research introduces a less computational cost and compact model to detect diseases in plant leaves. The proposed model functions to identify diseases, utilizing a dataset comprising 3,800 photographs of chilli leaves, categorized into six distinct classes: five disorder classes and one healthy chilli class. Through experimentation, the outcomes indicate that the suggested model achieves an accuracy of 98.09 %, along with a precision of 97 %, a recall of 97.25 %, and an F1 score of 97.25%. The presented approach demonstrates its superiority over existing methodologies.
Why it matches plant phenotyping methods唐辛子葉の画像から病害状態を分類する深層学習手法を開発・評価しており、植物病害フェノタイピング手法が研究の中心です。
abstractwe proposed a novel Residual Nested Dilated DenseNet based deep-learning (RNDDNet) model in this paper.
Feeding the increasing global population and reducing the carbon footprint of agricultural activities are two critical challenges of our century. Growing crops under protected horticulture and precise crop monitoring have emerged to address these challenges. Crop monitoring in commercial protected facilities remains mostly manual and labour intensive. Using computer vision to solve specific problems in image-based crop monitoring in these compact and complex growth environments is currently hindered by the scarcity of available data. We collected an RGBD dataset for vertically supported, hydroponically-grown capsicum plants in a commercial-scale glasshouse facility to fill this gap. Data were collected weekly using a single top-angled stereo camera mounted on a mobile platform running between the hydroponic gutters. The RGBD streams covered 80 % of the crop growing season in three different light conditions. The metadata include camera configurations and light condition information. Manually measured plant heights of ten selected plants per gutter are provided as ground truth. The images covered the whole plants and focused on the top third. This dataset will support research on plant height estimation, plant organ identification, object segmentation, organ measurements, 3D reconstruction, 3D data processing, and depth noise reduction. The usability of the dataset has been successfully demonstrated in a previously published study on plant height estimation using machine learning and 3D point cloud.
Why it matches plant phenotyping methods植物の草丈推定や器官計測を目的としたRGBD画像データセットを構築し、地上真値も提供しているため、植物フェノタイピング手法・データセットが中心です。
abstractWe collected an RGBD dataset for vertically supported, hydroponically-grown capsicum plants in a commercial-scale glasshouse facility to fill this gap.
Reproduction assets foundThis data article directly deposits its paper-specific phenotyping assets: the PC4C_CAPSI RGBD image dataset (Rosbag streams, JSON metadata, manual plant-height ground truth) on the Western Sydney University ResearchDirect repository, and the authors' RGBD processing code (image extraction, depth correction, 3D reconssDataset · publicData accessibility
Repository name: Image Data of Capsicum Plant Growth in Protected Horticulture: PC4C_CAPSI. [ 1 ]
Data identification number: 10.26183/1A0R-E318
Direct URL to data: https://rds.westernsydney.edu.au/Institutes/HIE/2024/Jayasuriya_N/Open asset ↗rds.westernsydney.edu.aulines:1-40Plant phenotyping relevance match · UnverifiedEurope PMC · checked 15 Sept 2026
Greenhouse production of fruits and vegetables in developed countries is challenged by labour scarcity and high labour costs. Robots offer a good solution for sustainable and cost-effective production. Acquiring accurate spatial information about relevant plant parts is vital for successful robot operation. Robot perception in greenhouses is challenging due to variations in plant appearance, viewpoints, and illumination. This paper proposes a keypoint-detection-based method using data from an RGB-D camera to estimate the 3D pose of peduncle nodes, which provides essential information to harvest the tomato bunches. Specifically, this paper proposes a method that detects four anatomical landmarks in the colour image and then integrates 3D point-cloud information to determine the 3D pose. A comprehensive evaluation was conducted in a commercial greenhouse to gain insight into the performance of different parts of the method. The results showed: (1) high accuracy in object detection, achieving an Average Precision (AP) of AP@0.5=0.96; (2) an average Percentage of Detected Joints (PDJ) of the keypoints of PhDJ@0.2 = 94.31%; and (3) 3D pose estimation accuracy with mean absolute errors (MAE) of 11ᵒ and 10ᵒ for the relative upper and lower angles between the peduncle and main stem, respectively. Furthermore, the capability to handle variations in viewpoint was investigated, demonstrating the method was robust to view changes. However, canonical and higher views resulted in slightly higher performance compared to other views. Although tomato was selected as a use case, the proposed method has the potential to be applied to other greenhouse crops, such as pepper, after fine-tuning.
Why it matches plant phenotyping methodsRGB-D画像と深度点群を用いてトマトの器官ランドマークを検出し、ペドンクル節の3D姿勢を推定する手法を開発・評価しており、植物形態の取得が中心である。
abstractThis paper proposes a keypoint-detection-based method using data from an RGB-D camera to estimate the 3D pose of peduncle nodes
Early disease detection is crucial for maximizingcrop yields and minimizing financial losses in agriculture. Inorder to categorise leaf diseases in pepper, tomato, and potato plants, this study suggests a revolutionary CNN design. The algorithm collects disease-specific features from input photos by using data augmentation techniques to artificially enlarge the training dataset. ReLU-activated convolutional layers gradually capture features at different levels, from low to high, while max pooling layers minimise spatial dimensionality. After thecharacteristics are extracted, a fully-connected layers with a softmax activation function classify them into illness groups. A well chosen dataset containing labelled healthy and sick leaves is used to train and validate the model. Generalizability to untesteddata is ensured via performance evaluation on an independent testing set. The field of deep learning for agricultural applications benefits from this research. This strategy has the ability to rev- olutionise agricultural practices by enabling automated disease identification. This might result in early interventions, better crop health, and eventually sustainable agricultural production.
Why it matches plant phenotyping methods植物葉の画像から病害状態をCNNで推定する手法の開発・検証が研究の中心であり、植物病害フェノタイピングに該当する。
abstractA well chosen dataset containing labelled healthy and sick leaves is used to train and validate the model.
Pepper is a high-economic-value agricultural crop that faces diverse disease challenges such as blight and anthracnose. These diseases not only reduce the yield of pepper but, in severe cases, can also cause significant economic losses and threaten food security. The timely and accurate identification of pepper diseases is crucial. Image recognition technology plays a key role in this aspect by automating and efficiently identifying pepper diseases, helping agricultural workers to adopt and implement effective control strategies, alleviating the impact of diseases, and being of great importance for improving agricultural production efficiency and promoting sustainable agricultural development. In response to issues such as edge-blurring and the extraction of minute features in pepper disease image recognition, as well as the difficulty in determining the optimal learning rate during the training process of traditional pepper disease identification networks, a new pepper disease recognition model based on the TPSAO-AMWNet is proposed. First, an Adaptive Residual Pyramid Convolution (ARPC) structure combined with a Squeeze-and-Excitation (SE) module is proposed to solve the problem of edge-blurring by utilizing adaptivity and channel attention; secondly, to address the issue of micro-feature extraction, Minor Triplet Disease Focus Attention (MTDFA) is proposed to enhance the capture of local details of pepper leaf disease features while maintaining attention to global features, reducing interference from irrelevant regions; then, a mixed loss function combining Weighted Focal Loss and L2 regularization (WfrLoss) is introduced to refine the learning strategy during dataset processing, enhancing the model's performance and generalization capabilities while preventing overfitting. Subsequently, to tackle the challenge of determining the optimal learning rate, the tent particle snow ablation optimizer (TPSAO) is developed to accurately identify the most effective learning rate. The TPSAO-AMWNet model, trained on our custom datasets, is evaluated against other existing methods. The model attains an average accuracy of 93.52% and an F1 score of 93.15%, demonstrating robust effectiveness and practicality in classifying pepper diseases. These results also offer valuable insights for disease detection in various other crops.
Why it matches plant phenotyping methodsコショウ葉の病害状態を画像から分類する新規認識モデルを開発し、既存手法との比較評価も行っており、植物フェノタイピング手法が中心である。
abstracta new pepper disease recognition model based on the TPSAO-AMWNet is proposed
Introduction Yunnan Xiaomila is a pepper variety whose flowers and fruits become mature at the same time and multiple times a year. The distinction between the fruits and the background is low and the background is complex. The targets are small and difficult to identify. Methods This paper aims at the problem of target detection of Yunnan Xiaomila under complex background environment, in order to reduce the impact caused by the small color gradient changes between xiaomila and background and the unclear feature information, an improved PAE-YOLO model is proposed, which combines the EMA attention mechanism and DCNv3 deformable convolution is integrated into the YOLOv8 model, which improves the model's feature extraction capability and inference speed for Xiaomila in complex environments, and achieves a lightweight model. First, the EMA attention mechanism is combined with the C2f module in the YOLOv8 network. The C2f module can well extract local features from the input image, and the EMA attention mechanism can control the global relationship. The two complement each other, thereby enhancing the model's expression ability; Meanwhile, in the backbone network and head network, the DCNv3 convolution module is introduced, which can adaptively adjust the sampling position according to the input feature map, contributing to stronger feature capture capabilities for targets of different scales and a lightweight network. It also uses a depth camera to estimate the posture of Xiaomila, while analyzing and optimizing different occlusion situations. The effectiveness of the proposed method was verified through ablation experiments, model comparison experiments and attitude estimation experiments. Results The experimental results indicated that the model obtained an average mean accuracy (mAP) of 88.8%, which was 1.3% higher than that of the original model. Its F1 score reached 83.2, and the GFLOPs and model sizes were 7.6G and 5.7MB respectively. The F1 score ranked the best among several networks, with the model weight and gigabit floating-point operations per second (GFLOPs) being the smallest, which are 6.2% and 8.1% lower than the original model. The loss value was the lowest during training, and the convergence speed was the fastest. Meanwhile, the attitude estimation results of 102 targets showed that the orientation was correctly estimated exceed 85% of the cases, and the average error angle was 15.91°. In the occlusion condition, 86.3% of the attitude estimation error angles were less than 40°, and the average error angle was 23.19°. Discussion The results show that the improved detection model can accurately identify Xiaomila targets fruits, has higher model accuracy, less computational complexity, and can better estimate the target posture.
Why it matches plant phenotyping methods唐辛子果実の検出に加え、深度カメラによる姿勢推定手法を開発し、アブレーション・比較・姿勢推定実験で技術検証しているため、植物器官の表現型取得が中心である。
abstractan improved PAE-YOLO model is proposed
Plant phenotyping relevance match · UnverifiedCrossref · Europe PMC · checked 15 Sept 2026
This article presents the chili and onion leaf (COLD) dataset, which focuses on the leaves of chili and onion plants, scientifically known as Allium cepa and capsicum. The presence of various diseases such as Purple blotch, Stemphylium leaf blight, Colletotrichum leaf blight, and Iris yellow spot virus in onions, as well as Cercospora leaf spot, powdery mildew, Murda complex syndrome, and nutrition deficiency in chili, have had a significant negative effect on onion and chili production. As a consequence, farmers have incurred financial losses. Computer vision and image-processing algorithms have been widely used in recent years for a range of applications, such as diagnosing and categorizing plant leaf diseases. In this paper we introduced a detailed chilli and onion leaf dataset gathered from Chilwadigi village with varying climatic conditions in Karnataka. The dataset contains a variety of chili and onion leaf categories carefully selected to tackle the complex challenges of categorizing leaf images taken in natural environments. Dealing with challenges such as subtle inter-class similarities, changes in lighting, and differences in background conditions like different foliage arrangements and varying light levels. We carefully documented chilli and onion leaves from various angles using high resolution camera to create a diverse and reliable dataset. The dataset on chilli leaves is set to be a valuable resource for enhancing computer vision algorithms, from traditional deep learning models to cutting-edge vision transformer architectures. This will help in creating advanced image recognition systems specifically designed for identifying chilli plants. By making this dataset publicly accessible, our goal is to empower researchers to develop new computer vision techniques to tackle the unique challenges of chilli and onion leaf recognition. You can access the dataset for free at the following DOI number: http://doi.org/10.17632/7nxxn4gj5s.3 and http://doi.org/10.17632/tf9dtfz9m6.3.
Why it matches plant phenotyping methods植物葉画像から病害状態を識別する公開データセットの構築が中心であり、画像ベース植物フェノタイピング用の再利用可能な資源に該当する。
abstractThis article presents the chili and onion leaf (COLD) dataset
Currently, foliar diseases of chili have significantly impacted both yield and quality. Despite effective advancements in deep learning techniques for the classification of chili leaf diseases, most existing classification models still face challenges in terms of accuracy and practical application in disease identification. Therefore, in this study, an optimized and enhanced convolutional neural network model named MCCM (MCSAM-ConvNeXt-MSFFM) is proposed by introducing ConvNeXt. The model incorporates a Multi-Scale Feature Fusion Module (MSFFM) aimed at better capturing disease features of various sizes and positions within the images. Moreover, adjustments are made to the positioning, activation functions, and normalization operations of the MSFFM module to further optimize the overall model. Additionally, a proposed Mixed Channel Spatial Attention Mechanism (MCSAM) strengthens the correlation between non-local channels and spatial features, enhancing the model's extraction of fundamental characteristics of chili leaf diseases. During the training process, pre-trained weights are obtained from the Plant Village dataset using transfer learning to accelerate the model's convergence. Regarding model evaluation, the MCCM model is compared with existing CNN models (Vgg16, ResNet34, GoogLeNet, MobileNetV2, ShuffleNet, EfficientNetV2, ConvNeXt), and Swin-Transformer. The results demonstrate that the MCCM model achieves average improvements of 3.38%, 2.62%, 2.48%, and 2.53% in accuracy, precision, recall, and F1 score, respectively. Particularly noteworthy is that compared to the original ConvNeXt model, the MCCM model exhibits significant enhancements across all performance metrics. Furthermore, classification experiments conducted on rice and maize disease datasets showcase the MCCM model's strong generalization performance. Finally, in terms of application, a chili leaf disease classification website is successfully developed using the Flask framework. This website accurately identifies uploaded chili leaf disease images, demonstrating the practical utility of the model.
Why it matches plant phenotyping methodsトウガラシ葉の病徴を画像から分類する深層学習手法を開発・比較評価しており、植物の病害状態を推定するフェノタイピング手法が中心である。
abstractan optimized and enhanced convolutional neural network model named MCCM (MCSAM-ConvNeXt-MSFFM) is proposed
Reproduction assets foundThe paper's phenotyping inputs are public Kaggle image datasets: the chili leaf disease dataset used as the primary experimental data, plus rice and maize leaf disease datasets used for generalization tests. No author analysis code, trained model checkpoints, or dataset deposits are stated; the Flask website is a live-Dataset · publicn of experiments. Section 4 analyzes all research findings, proposes possible improvements, and suggests future research directions. Finally, Section 5 summarizes the main contributions of this study.
2
Materials and methods
2.1
Datasets
The chili leaf disease dataset is sourced from publicly available datasets on the internet( https://www.kaggle.com/datasets/dhenyd/chili-plant-disease/ ). The initial dataset consists of 500 images, encompassing five categories: healthy chili leaves, leaf curl disease, leaf spot disease, whitefly, and yellowing disease. Samples of each category are illustrated in
Figure 1
.
Figure 1
Chili samples. (A) Healthy chili leaves. (B) Leaf curl disease. (C) Leaf spoOpen asset ↗Kaggle · dhenyd/chili-plant-diseaselines:59-78Dataset · publicthe corresponding accuracy rate of each cross-validation.
3.10.2
Rice and maize disease test results
To validate the generalization ability of the MCCM model on different crop diseases, this study conducted performance tests using publicly available datasets, including rice leaf disease dataset and maize leaf disease dataset ( https://www.kaggle.com/datasets/nirmalsankalana/rice-leaf-disease-image and https://www.kaggle.com/datasets/smaranjitghose/corn-or-maize-leaf-disease-dataset ). The rice leaf disease dataset covers four types: rice blast, bacterial leaf blight, brown spot, and rice tungro disease, while the maize leaf disease dataset includes healthy states and three types of diseases:Open asset ↗Kaggle · nirmalsankalana/rice-leaf-disease-imagelines:456-477Dataset · publicaize disease test results
To validate the generalization ability of the MCCM model on different crop diseases, this study conducted performance tests using publicly available datasets, including rice leaf disease dataset and maize leaf disease dataset ( https://www.kaggle.com/datasets/nirmalsankalana/rice-leaf-disease-image and https://www.kaggle.com/datasets/smaranjitghose/corn-or-maize-leaf-disease-dataset ). The rice leaf disease dataset covers four types: rice blast, bacterial leaf blight, brown spot, and rice tungro disease, while the maize leaf disease dataset includes healthy states and three types of diseases: gray leaf spot, rust, and leaf blight. To ensure dataset diversity, data aOpen asset ↗Kaggle · smaranjitghose/corn-or-maize-leaf-disease-datasetlines:456-477Plant phenotyping relevance match · UnverifiedCrossref · Europe PMC · checked 14 Sept 2026
Why it matches plant phenotyping methods赤外線熱画像から pepper 葉の温度分布指標を抽出し、無症状期の病徴を検出・評価する手法が研究の中心であるため、植物病害フェノタイピング手法として含める。
abstractThe present study confirms the feasibility of the identification of presymptomatic features in pepper early blight by infrared thermography during the incubation period.
Pepper leaf segmentation plays a crucial role in monitoring pepper leaf diseases in various backgrounds and ensuring the healthy growth of peppers. However, existing transformer-based segmentation methods suffer from computational inefficiency, excessive parameterization, and limited utilization of edge information. To tackle these challenges, we propose an adaptive multi-scale MLP framework, named AMS-MLP, which combines the multi-path aggregation module (MPAM) and the multi-scale context relation mask module (MCRD) to refine the object boundaries in pepper leaf segmentation. AMS-MLP consists of an encoder-based network, an adaptive multi-scale MLP (AM-MLP) module, and a decoder network. In the encoder network, the MPAM module effectively fuses five-scale features to generate a single-channel mask, improving the accuracy of pepper leaf boundary extraction. The AM-MLP module divides the input features into two branches: the global multi-scale MLP branch captures long-range dependencies between image information, while the local multi-scale MLP branch focuses on extracting local feature maps. Adaptive attention mechanism is designed to dynamically adjust the weights of global and local features. The decoder network incorporates the MCRD module into the convolutional layer, enhancing the extraction of boundary features. To verify the performance of the proposed method, we conducted extensive experiments on three pepper leaf datasets with different backgrounds. The results demonstrate mIoU scores of 97.39%, 96.91%, and 97.91%, as well as F1 scores of 98.29%, 97.86%, and 98.51%, respectively. Comparative analysis with U-Net and state-of-the-art models reveals that the proposed method dramatically improves the accuracy and efficiency of pepper leaf image segmentation.
Why it matches plant phenotyping methodsコショウ葉の画像から葉領域・境界を抽出するセグメンテーション手法を開発し、複数データセットで性能検証しているため、植物表現型取得法が中心である。
abstractwe propose an adaptive multi-scale MLP framework, named AMS-MLP, which combines the multi-path aggregation module (MPAM) and the multi-scale context relation mask module (MCRD) to refine the object boundaries in pepper leaf segmentation.
Reproduction assets foundThe paper reports pepper leaf segmentation on author-collected PLID datasets (EBD, BSD, MLD) and states in Supplementary Materials that the authors' analysis code will be available at a public GitHub URL matching an allowed URL. The datasets themselves have no public deposit (Data Availability Statement directs furtherCode · publicod for
pepper leaf segmentation. On the other hand, we investigate fine-tuning methods on existing deep
learning-based models to enhance the generalisation ability of the models and provide more effective
and feasible solutions for pepper leaf images in different scenarios
Supplementary Materials: The code will be available at: https://github.com/fangchj2002/AMS-MLP.
Author Contributions: All authors contributed to the article and JF: conceptualization, methodology,
experiment, and writing. JY: experiment and writing. HL: supervision and writing- review & editing.YF:
methodology and approved the submitted version.
Funding: The research described in this paper was funded by the National NatOpen asset ↗fangchj2002/AMS-MLPpdf-layout-page:20 lines:1-66Plant phenotyping relevance match · UnverifiedCrossref · checked 13 Sept 2026
Abstract Plants generate electrical signals in response to mild and severe environmental stimuli to transmit physiological information and ultimately trigger defensive responses during stressful events. It has been proposed that detecting and characterizing such signals could allow researchers to mimic specific electrical stimuli and provoke desirable responses in crops. Nevertheless, manually inserting electrodes in plant tissues leads to irregular data records due to a lack of uniformity across insertion events. For this reason, we manufactured a prototype of an electrode/needle insertion mechanism built in aluminum and acrylic and used it to measure electrical signals caused by drought in Capsicum annum plants. As results the mechanism had a more consistent performance in the characteristics of the insertion as depth, alignment between electrodes and with plant stem. As well, the mechanism was used for obtaining electrical signals (ES) related to drought, which, through a convolutional neural network (CNN) could be differentiated from control ES´s with an 84.91% recognition rate. It was concluded that the mechanism reduced variations in the characteristics of the electrode insertion and that it could be implemented for ES´s analysis.
Why it matches plant phenotyping methods植物への電極挿入を標準化する機構を開発・評価し、乾燥ストレスに伴う植物電気シグナルの取得と分類に用いており、表現型取得法が中心的です。
abstractwe manufactured a prototype of an electrode/needle insertion mechanism built in aluminum and acrylic and used it to measure electrical signals caused by drought in Capsicum annum plants.
Vertical Indoor Farming (VIF) with hydroponics offers a promising perspective for sustainable food production. Intelligent control of VIF system components plays a key role in reducing operating costs and increasing crop yields. Modern machine vision (MV) systems use Deep Learning (DL) in combination with camera systems for various tasks in agriculture, such as disease and nutrient deficiency detection, and flower and fruit identification and classification for pollination and harvesting. This study presents the applicability of MV technology with DL modelling to detect the growth stages of chilli plants using YOLOv8 networks. The influence of different bird’s eye and side view datasets and different YOLOv8 architectures was analysed. To generate the image data for training and testing the YOLO models, chilli plants were grown in a hydroponic environment and imaged throughout their life cycle using four camera systems. The growth stages were divided into growing, flowering and fruiting classes. All trained YOLOv8 models showed reliable identification of growth stages with high accuracy. The results indicate that models trained with data from both views show better generalisation. YOLO’s middle architecture achieved the best performance.
Why it matches plant phenotyping methods機械視覚とYOLOv8を用いて、トウガラシの生育段階を画像から推定する手法を開発・比較しており、植物状態の取得・抽出が研究の中心である。
abstractThis study presents the applicability of MV technology with DL modelling to detect the growth stages of chilli plants using YOLOv8 networks.
Published2 Apr 20242024 International Conference on Science, Engineering and Business for Driving Sustainable Development Goals (SEB4SDG)Cited by 3 · OpenAlex ↗
The application of Deep Learning (DL) and Machine Learning (ML) has significantly improved the processing of images in the past few years over traditional approaches. With the ever-growing world population and increased demand for food, global food production depends heavily on plants. Plant diseases are caused by a variety of environmental variables, which significantly reduce productivity. This poses an urgent threat to food security, yet in several parts of the world, it is still challenging to quickly identify them. Traditional methods of detecting and preventing the spread of plant disease have become less efficient, inaccurate, and time-intensive, thus an automated and reliable approach to detecting these diseases is imperative. By enabling early detection of plant diseases, adopting cutting-edge technologies like ML and DL can assist in overcoming these difficulties. This study aimed to design a Convolutional Neural Network (CNN) model for detecting diseases in plants using publicly available plant leaf datasets obtained from Kaggle. The datasets contain Tomato, Potato, and Pepper bell leaves that are diseased and healthy. The efficacy of the model was assessed utilising precision, recall, and F1 score. CNN had an accuracy of 94%. Thus, the detection and proper diagnosis of plant disease is principal for successful plant growth and this reduces the threats it poses to food security in the world. It is recommended that the proposed deep learning model be translated into a system suitable for plant disease detection.
Why it matches plant phenotyping methods植物葉画像から病害状態を推定するCNNモデルの設計と性能評価が研究の中心であり、植物病害という表現型状態を直接推定しているため。
abstractThis study aimed to design a Convolutional Neural Network (CNN) model for detecting diseases in plants using publicly available plant leaf datasets obtained from Kaggle.
Pepper / chilliField / plotFruitClassificationObject detectionGrowth / development / phenology
In recent years, the accurate identification of chili maturity stages has become essential for optimizing cultivation processes. Conventional methodologies, primarily reliant on manual assessments or rudimentary detection systems, often fall short of reflecting the plant’s natural environment, leading to inefficiencies and prolonged harvest periods. Such methods may be imprecise and time-consuming. With the rise of computer vision and pattern recognition technologies, new opportunities in image recognition have emerged, offering solutions to these challenges. This research proposes an affordable solution for object detection and classification, specifically through version 5 of the You Only Look Once (YOLOv5) model, to determine the location and maturity state of rocoto chili peppers cultivated in Ecuador. To enhance the model’s efficacy, we introduce a novel dataset comprising images of chili peppers in their authentic states, spanning both immature and mature stages, all while preserving their natural settings and potential environmental impediments. This methodology ensures that the dataset closely replicates real-world conditions encountered by a detection system. Upon testing the model with this dataset, it achieved an accuracy of 99.99% for the classification task and an 84% accuracy rate for the detection of the crops. These promising outcomes highlight the model’s potential, indicating a game-changing technique for chili small-scale farmers, especially in Ecuador, with prospects for broader applications in agriculture. Doi: 10.28991/ESJ-2024-08-02-08 Full Text: PDF
Why it matches plant phenotyping methodsYOLOv5による画像解析で、トウガラシ果実の位置と成熟段階という植物形質を推定する手法を開発・評価しており、データセット構築と性能検証も中心的である。
abstractThis research proposes an affordable solution for object detection and classification, specifically through version 5 of the You Only Look Once (YOLOv5) model, to determine the location and maturity state of rocoto chili peppers cultivated in Ecuador.
This research paper explores a comprehensive approach to advancing capsicum harvesting by integrating cutting-edge technologies. The study addresses key objectives, including capsicum detection using various YOLO algorithms, peduncle detection through YOLO segmentation models in a proposed robotic harvester, laboratory testing of cutting target point coordinates using the Real Sense D455 RGB-D camera, growth stage determination, and capsicum counting/tracking with a supervision algorithm. The investigation highlights the YOLOv8s model as the most successful for capsicum detection, achieving a remarkable mean Average Precision (mAP) of 0.967 at a 0.5 Intersection over Union (IOU) threshold. As part of the growth stage determination task, YOLOv8s achieved a satisfactory mAP of 0.614 at the same IOU threshold. Additionally, the YOLOv8s-seg model demonstrated superior performance in peduncle detection, attaining a box mAP of 0.790 and a mask mAP of 0.771. The YOLOv8s-seg model excels in peduncle detection with a box mAP of 0.790 and a mask mAP of 0.771. Laboratory experiments using the Real Sense D455 RGB-D camera showcased its capability to localize the target point with a maximum error of 8 mm longitudinally, 9 mm vertically, and 12 mm laterally. The developed tracking and counting algorithm achieve a notable counting accuracy of 94.1 % during the third harvesting cycle in the greenhouse. The Android application developed demonstrated robust performance, achieving high accuracy (Mean IoU: 0.92), precise localization (Mean Euclidean Distance: 5 pixels), responsive user interface (Touch Response Time: 150 ms), and broad compatibility across Android versions and device types, with effective error handling (Success Rate: 95 %). The study not only advances capsicum harvesting techniques but also presents practical insights for the integration of advanced technologies, paving the way for efficient robotic harvesting systems in agriculture.
Why it matches plant phenotyping methodsYOLO画像解析、成長段階分類、果実カウント・追跡を統合した植物状態・器官形質の取得手法を開発し、精度を評価しているため、収穫ロボットの単なる対象定位を超える中心的なフェノタイピング研究である。
abstractThe study addresses key objectives, including capsicum detection using various YOLO algorithms, peduncle detection through YOLO segmentation models in a proposed robotic harvester, laboratory testing of cutting target point coordinates using the Real Sense D455 RGB-D camera, growth stage determination, and capsicum counting/tracking with a supervision algorithm.
Accurate collection of plant phenotyping is critical to optimising sustainable farming practices in precision agriculture. Traditional phenotyping in controlled laboratory environments, while valuable, falls short in understanding plant growth under real-world conditions. Emerging sensor and digital technologies offer a promising approach for direct phenotyping of plants in farm environments. This study investigates a learning-based phenotyping method using the Neural Radiance Field to achieve accurate in-situ phenotyping of pepper plants in greenhouse environments. To quantitatively evaluate the performance of this method, traditional point cloud registration on 3D scanning data is implemented for comparison. Experimental result shows that NeRF(Neural Radiance Fields) achieves competitive accuracy compared to the 3D scanning methods. The mean distance error between the scanner-based method and the NeRF-based method is 0.865mm. This study shows that the learning-based NeRF method achieves similar accuracy to 3D scanning-based methods but with improved scalability and robustness.
Why it matches plant phenotyping methodsNeRFを用いた植物の3D表現・形質取得法を開発し、3Dスキャン法との精度比較で検証しており、フェノタイピング手法が研究の中心である。
abstractThis study investigates a learning-based phenotyping method using the Neural Radiance Field to achieve accurate in-situ phenotyping of pepper plants in greenhouse environments.
Plant phenotyping relevance match · UnverifiedCrossref · Europe PMC · checked 7 Sept 2026
Crop leaf length, perimeter, and area serve as vital phenotypic indicators of crop growth status, the measurement of which is important for crop monitoring and yield estimation. However, processing a leaf point cloud is often challenging due to cluttered, fluctuating, and uncertain points, which culminate in inaccurate measurements of leaf phenotypic parameters. To tackle this issue, the RKM-D point cloud method for measuring leaf phenotypic parameters is proposed, which is based on the fusion of improved Random Sample Consensus with a ground point removal (R) algorithm, the K-means clustering (K) algorithm, the Moving Least Squares (M) method, and the Euclidean distance (D) algorithm. Pepper leaves were obtained from three growth periods on the 14th, 28th, and 42nd days as experimental subjects, and a stereo camera was employed to capture point clouds. The experimental results reveal that the RKM-D point cloud method delivers high precision in measuring leaf phenotypic parameters. (i) For leaf length, the coefficient of determination (R2) surpasses 0.81, the mean absolute error (MAE) is less than 3.50 mm, the mean relative error (MRE) is less than 5.93%, and the root mean square error (RMSE) is less than 3.73 mm. (ii) For leaf perimeter, the R2 surpasses 0.82, the MAE is less than 7.30 mm, the MRE is less than 4.50%, and the RMSE is less than 8.37 mm. (iii) For leaf area, the R2 surpasses 0.97, the MAE is less than 64.66 mm2, the MRE is less than 4.96%, and the RMSE is less than 73.06 mm2. The results show that the proposed RKM-D point cloud method offers a robust solution for the precise measurement of crop leaf phenotypic parameters.
Why it matches plant phenotyping methods葉の形態形質を点群から抽出するRKM-D法を開発し、精度を検証した研究であり、植物フェノタイピング手法が中心である。
abstractthe RKM-D point cloud method for measuring leaf phenotypic parameters is proposed
Plant phenotyping relevance match · UnverifiedCrossref · Europe PMC · checked 7 Sept 2026
Accurate and rapid plant disease detection is critical for enhancing long-term agricultural yield. Disease infection poses the most significant challenge in crop production, potentially leading to economic losses. Viruses, fungi, bacteria, and other infectious organisms can affect numerous plant parts, including roots, stems, and leaves. Traditional techniques for plant disease detection are time-consuming, require expertise, and are resource-intensive. Therefore, automated leaf disease diagnosis using artificial intelligence (AI) with Internet of Things (IoT) sensors methodologies are considered for the analysis and detection. This research examines four crop diseases: tomato, chilli, potato, and cucumber. It also highlights the most prevalent diseases and infections in these four types of vegetables, along with their symptoms. This review provides detailed predetermined steps to predict plant diseases using AI. Predetermined steps include image acquisition, preprocessing, segmentation, feature selection, and classification. Machine learning (ML) and deep understanding (DL) detection models are discussed. A comprehensive examination of various existing ML and DL-based studies to detect the disease of the following four crops is discussed, including the datasets used to evaluate these studies. We also provided the list of plant disease detection datasets. Finally, different ML and DL application problems are identified and discussed, along with future research prospects, by combining AI with IoT platforms like smart drones for field-based disease detection and monitoring. This work will help other practitioners in surveying different plant disease detection strategies and the limits of present systems.
Why it matches plant phenotyping methods植物病害の画像取得・分割・特徴抽出・分類による症状検出手法を中心にレビューしており、植物状態の推定方法が主題である。
abstractThis review provides detailed predetermined steps to predict plant diseases using AI. Predetermined steps include image acquisition, preprocessing, segmentation, feature selection, and classification.
In the field of agriculture, measuring the leaf area is crucial for the management of crops. Various techniques exist for this measurement, ranging from direct to indirect approaches and destructive to non-destructive techniques. The non-destructive approach is favored because it preserves the plant’s integrity. Among these, several methods utilize leaf dimensions, such as width and length, to estimate leaf areas based on specific models that consider the unique shapes of leaves. Although this approach does not damage plants, it is labor-intensive, requiring manual measurements of leaf dimensions. In contrast, some indirect non-destructive techniques leveraging convolutional neural networks can predict leaf areas more swiftly and autonomously. In this paper, we propose a new direct method using 3D point clouds constructed by semantic RGB-D (Red Green Blue and Depth) images generated by a semantic segmentation neural network and RGB-D images. The key idea is that the leaf area is quantified by the count of points depicting the leaves. This method demonstrates high accuracy, with an R2 value of 0.98 and a RMSE (Root Mean Square Error) value of 3.05 cm2. Here, the neural network’s role is to segregate leaves from other plant parts to accurately measure the leaf area represented by the point clouds, rather than predicting the total leaf area of the plant. This method is direct, precise, and non-invasive to sweet pepper plants, offering easy leaf area calculation. It can be implemented on laptops for manual use or integrated into robots for automated periodic leaf area assessments. This innovative method holds promise for advancing our understanding of plant responses to environmental changes. We verified the method’s reliability and superior performance through experiments on individual leaves and whole plants.
Why it matches plant phenotyping methodsRGB-D画像と意味分割による3D点群から葉面積を直接推定する手法を開発・検証しており、植物表現型の取得が研究の中心です。
abstractIn this paper, we propose a new direct method using 3D point clouds constructed by semantic RGB-D (Red Green Blue and Depth) images generated by a semantic segmentation neural network and RGB-D images.
Plant phenotyping relevance match · UnverifiedCrossref · Europe PMC · OpenAlex · checked 15 Sept 2026
The increasing demand for quality, year-round food production in limited space has led to the widespread adoption of protected cropping. Effectively monitoring and maintaining crops within these facilities requires substantial labour and expertise. Traditional manual monitoring is labour intensive and time consuming. Therefore, non-destructive image-based techniques, particularly those utilising 3D structural data, have gained attention. We developed a stereo vision-based system to estimate the height of vertically supported tall plants in protected facilities, given plant height serves as a vital measure of crop growth. Our system uses a mobile platform with a top-angle view of a stereo vision depth camera for data acquisition and machine learning in its core for data analysis. First, we collected weekly RGB and depth (RGBD) streams from plant gutters in three glasshouse compartments with different light treatments. We used part of the RGB data collected to train and validate a deep learning segmentation model to detect plant tops and bases. Detected tops and bases of an image were then mapped to the generated 3D scene using the depth image of the same frame. Thresholds and 3D clustering are used respectively to remove background and eliminate outliers in top and base detection mapped to 3D space. Finally, the height of each plant was calculated using the cluster centres of the tops and bases of the plants. Manually measured heights of ten selected plants per environment were used to validate the height estimations. Similar growing patterns were observed between imaged and manually measured plant heights, which showed strong correlations of 0.87, 0.96, and 0.79 R2 scores, respectively, under unfiltered ambient light, Smart Glass film, and shifted light. These promising results demonstrate the feasibility of our proposed method for a vertically supported capsicum crop in a commercial-scale protected crop facility.
Why it matches plant phenotyping methodsステレオビジョンと機械学習を用いて植物体高を推定する手法を開発し、手動測定で検証しており、植物表現型の取得が研究の中心である。
abstractWe developed a stereo vision-based system to estimate the height of vertically supported tall plants in protected facilities
Abstract Agriculture serves as a vital sector in Tunisia, supporting the nation's economy and ensuring food production. However, the detrimental impact of plant diseases on crop yield and quality presents a significant challenge for farmers. In this context, computer vision techniques have emerged as promising tools for automating disease detection processes. This paper focuses on the application of the YOLOv5 algorithm for the simultaneous detection and localization of multiple plant diseases on leaves. By using a self-generated dataset and employing techniques such as augmentation, anchor clustering, and segmentation, the study aims to enhance detection accuracy. An ablation study comparing YOLOv5s and YOLOv5x models demonstrates the superior performance of YOLOv5x, achieving a mean average precision (mAP) of 96.5%.
Why it matches plant phenotyping methods葉の病害を画像から検出・局在化し、データセット、拡張、アンカー調整、セグメンテーション、モデル比較と精度評価を中心に扱うため、植物病害状態の画像ベース表現型計測手法として採用。
abstractThis paper focuses on the application of the YOLOv5 algorithm for the simultaneous detection and localization of multiple plant diseases on leaves.
Abstract Being capable of accurately predicting morphological parameters of the plant weeks before achieving fruit maturation is of great importance in the production and selection of suitable ornamental pepper plants. The objective of this article is evaluating the feasibility and assessing the performance of CNN-based models using RGB images as input to forecast two morphological parameters: plant height and canopy diameter. To this end, four CNN-based models are proposed to predict these morphological parameters in four different scenarios: first, using as input a single image of the plant; second, using as input several images from different viewpoints of the plant acquired on the same date; third, using as input two images from two consecutive weeks; and fourth, using as input a set of images consisting of one image from each week up to the current date. The results show that it is possible to accurately predict both plant height and canopy diameter. The RMSE for a forecast performed 6 weeks in advance to the actual measurements was below 4.5 cm and 4.2 cm, respectively. When information from previous weeks is added to the model, better results can be achieved and as the prediction date gets closer to the assessment date the accuracy improves as well.
Why it matches plant phenotyping methodsRGB画像からCNNで植物体高と樹冠径を予測し、予測精度を評価する手法開発・検証が研究の中心であるため。
abstractThe objective of this article is evaluating the feasibility and assessing the performance of CNN-based models using RGB images as input to forecast two morphological parameters: plant height and canopy diameter.
Reproduction assets foundThe paper's curated dataset of morphological measurements (plant height, canopy diameter) and weekly RGB photographs of the 15 Capsicum accessions is explicitly deposited on Zenodo with a DOI matching an allowed URL. No author analysis code or trained model checkpoints are stated as publicly available.Dataset · publicThe resulting dataset, already curated, has been made publicly available at Zenodo (Alves Barroso et al., 2024 ).Open asset ↗Zenodolines:146-227Code / dataset availability confirmedCrossref · checked 15 Sept 2026
This study presents an approach to address the challenges of recognizing the maturity stage and counting sweet peppers of varying colors (green, yellow, orange, and red) within greenhouse environments. The methodology leverages the YOLOv5 model for real-time object detection, classification, and localization, coupled with the DeepSORT algorithm for efficient tracking. The system was successfully implemented to monitor sweet pepper production, and some challenges related to this environment, namely occlusions and the presence of leaves and branches, were effectively overcome. We evaluated our algorithm using real-world data collected in a sweet pepper greenhouse. A dataset comprising 1863 images was meticulously compiled to enhance the study, incorporating diverse sweet pepper varieties and maturity levels. Additionally, the study emphasized the role of confidence levels in object recognition, achieving a confidence level of 0.973. Furthermore, the DeepSORT algorithm was successfully applied for counting sweet peppers, demonstrating an accuracy level of 85.7% in two simulated environments under challenging conditions, such as varied lighting and inaccuracies in maturity level assessment.
Why it matches plant phenotyping methods深層学習による果実の成熟段階認識と計数が研究の中心であり、植物器官の状態(成熟度)を画像から抽出・評価しているため、植物フェノタイピング手法として含める。
abstractThis study presents an approach to address the challenges of recognizing the maturity stage and counting sweet peppers of varying colors
Reproduction assets foundThe authors explicitly state their dataset and supporting data are publicly available via their own GitHub repository (YOLOv5 + DeepSORT sweet pepper detection/counting), with an exact URL given in the text and Data Availability Statement. The Kaggle and Roboflow datasets are cited external/prior datasets, not paper-.Dataset · publicData Availability Statement: The data that support the findings of this study are available on GitHub
via [41].Open asset ↗pdf-raw-page:29 lines:1-51Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
MaizePepper / chilliTomatoRootMorphology / geometry measurementRoot system architecture
Image-based root phenotyping technologies, including the minirhizotron (MR), have expanded our understanding of the in situ root responses to changing environmental conditions. The conventional manual methods used to analyze MR images are time-consuming, limiting their implementation. This study presents an adaptation of our previously developed convolutional neural network-based models to estimate the total (cumulative) root length (TRL) per MR image without requiring segmentation. Training data were derived from manual annotations in Rootfly, commonly used software for MR image analysis. We compared TRL estimation with 2 models, a regression-based model and a detection-based model that detects the annotated points along the roots. Notably, the detection-based model can assist in examining human annotations by providing a visual inspection of roots in MR images. The models were trained and tested with 4,015 images acquired using 2 MR system types (manual and automated) and from 4 crop species (corn, pepper, melon, and tomato) grown under various abiotic stresses. These datasets are made publicly available as part of this publication. The coefficients of determination ( R 2 ), between the measurements made using Rootfly and the suggested TRL estimation models were 0.929 to 0.986 for the main datasets, demonstrating that this tool is accurate and robust. Additional analyses were conducted to examine the effects of (a) the data acquisition system and thus the image quality on the models' performance, (b) automated differentiation between images with and without roots, and (c) the use of the transfer learning technique. These approaches can support precision agriculture by providing real-time root growth information.
Why it matches plant phenotyping methods画像から根長という植物形質を推定するCNN手法を開発し、複数データセットで精度・頑健性を検証しているため、植物フェノタイピング手法が中心です。
abstractThis study presents an adaptation of our previously developed convolutional neural network-based models to estimate the total (cumulative) root length (TRL) per MR image without requiring segmentation.
Reproduction assets foundThe authors explicitly deposited the 4,015 minirhizotron root images with Rootfly TRL and point-coordinate annotations used to train and test their CNN models in a public Zenodo repository, making it a directly qualifying paper-specific public dataset.Dataset · publicThe datasets generated and analyzed during the current study are available in the Zenodo repository, https://doi.org/10.5281/zenodo.7482146 .Open asset ↗Zenodo · 10.5281/zenodo.7482146lines:373-471Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
Habanero plant diseases can significantly reduce crop yield and quality, making early detection and treatment crucial for farmers. In this study, we discuss the creation of a modified VGG16 (MVGG16) Deep Transfer Learning (DTL) model-based smartphone app for identifying habanero plant diseases. With the help of the smartphone application, growers can quickly diagnose the health of a habanero plant by taking a photo of one of its leaves. We trained the DTL model on a dataset of labelled images of healthy and infected habanero plants and evaluated its performance on a separate test dataset. The MVGG16 DTL algorithm had an accuracy, precision, f1-score, recall and AUC of 98.79%, 97.93%, 98.44%, 98.95 and 98.63%, respectively, on the testing dataset. The MVGG16 DTL model was then integrated into a smartphone app that enables users to upload photographs, get diagnosed, and explore a history of earlier diagnoses. We tested the software on a collection of photos of habanero plant leaves and discovered that it was highly accurate at spotting infected plants. The smartphone software can boost early identification and treatment of habanero plant diseases, resulting in higher crop output and higher-quality harvests.
Why it matches plant phenotyping methods葉画像から感染状態を推定する深層学習モデルとスマートフォンアプリの開発・評価が研究の中心であり、植物病害状態の表現型計測に該当する。
abstractcreation of a modified VGG16 (MVGG16) Deep Transfer Learning (DTL) model-based smartphone app for identifying habanero plant diseases
Reproduction assets foundThe authors trained and evaluated their MVGG16 habanero disease-detection model on a public Kaggle image dataset (PlantVillage), explicitly linked in the Data availability statement. No author analysis code, trained model, or app source is publicly deposited.Dataset · publicWe gathered a dataset of habanero plant images, including 1478 images of healthy plants and 997 photos of plants with one of five common diseases: bacterial spot, anthracnose, powdery mildew, Phytophthora blight, and tomato spotted wilt virus as obtained from the Kaggle repository with link: https://www.kaggle.com/datasets/arjuntejaswi/plant-village?resource=downloadOpen asset ↗Kaggle · arjuntejaswi/plant-villagelines:82-91Code / dataset availability confirmedOpenAlex · Europe PMC · checked 14 Sept 2026
CucumberPepper / chilliTomatoThermalLeafPhysiological trait estimationPlant / canopy temperature
Image-based high-throughput phenotyping promises the rapid determination of functional traits in large plant populations. However, interpretation of some traits - such as those related to photosynthesis or transpiration rates - is only meaningful if the irradiance absorbed by the measured leaves is known, which can differ greatly between different parts of the same plant and within canopies. No feasible method currently exists to rapidly measure absorbed irradiance in three-dimensional plants and canopies. We developed a method and protocols to derive absorbed irradiance at any visible part of a canopy with a thermal camera, by fitting a leaf energy balance model to transient changes in leaf temperature. Leaves were exposed to short light pulses (30 s) that were not long enough to trigger stomatal opening but strong enough to induce transient changes in leaf temperature that was proportional to the absorbed irradiance. The method was successfully validated against point measurements of absorbed irradiance in plant species with relatively simple architecture (sweet pepper, cucumber, tomato, and lettuce). Once calibrated, the model was used to produce absorbed irradiance maps from thermograms. Our method opens new avenues for the interpretation of plant responses derived from imaging techniques and can be adapted to existing high-throughput phenotyping platforms.
Why it matches plant phenotyping methods熱画像と葉エネルギーバランスモデルにより、植物キャノピー内の吸収光量を迅速に推定・可視化するフェノタイピング手法を開発し、点測定で検証しているため。
abstractWe developed a method and protocols to derive absorbed irradiance at any visible part of a canopy with a thermal camera, by fitting a leaf energy balance model to transient changes in leaf temperature.
Reproduction assets foundThe paper's authors explicitly state that all analysis code (R/STAN energy-balance fitting and Julia absorbed-irradiance mapping) is publicly available on their GitHub repository, which directly reproduces the paper's phenotyping computations. No separate phenotype dataset or image deposit is stated in the supplied.Code · publicData analysis
Calculations to solve Eqns 2 and 3 were run in R (R project,
v.4.2.0). The absorbed irradiance map was calculated in JULIA
(v.1.40.1; https://julialang.org/). All codes are available on
GitHub (https://github.com/jiayu0903/leaf-absorbed-irradiance.git). Statistical analysis was performed using a Student’s t-test for
paired samples to determine significant differences (P < 0.05)
between means.
Results
The temperature of sweet pepper (Fig. 2a), cucumber (Fig. S6a),
and tomato leaves (Fig. S7) showed a near-linear increase when
exposed to a brief 30 s period of irradiance fromOpen asset ↗https://github.com/jiayu0903/leaf-absorbed-irradiance.gitpdf-raw-page:7 lines:1-87Plant phenotyping relevance match · UnverifiedEurope PMC · checked 14 Sept 2026
Jalapeño peppers (Capsicum annuum L.) are an important agricultural product worldwide. Despite its high demand in recent years, there are few studies on its production under adverse conditions caused by environmental phenomena. Crops in protected environments such as aeroponics offer greater control of these phenomena and enable efficient use of resources. However, it is necessary to validate the cultivation technique for which new technologies are being used. One of these is the analysis of images captured in the visible and near and far infrared spectrum which involves using non-invasive techniques in order to characterize the growth of a plant and diagnose if it presents any type of stress. This study presents the characterization of vegetative growth and fruiting of jalapeño pepper plants in an aeroponic system, where the root, leaf development parameters and fruits were measured in four jalapeño pepper crops through images of plants captured in the visible (VIS), near infrared (NIR) and far infrared (IR) spectrums. Four crops of thirty jalapeño pepper plants were sown to obtain a total of one hundred and twenty plants which were characterized in the different phases of growth and fruiting. Each of the four jalapeño pepper crops were monitored for sixty days in an aeroponic system in a greenhouse. The first crop was intended to carry out tests to establish the appropriate fertigation times, the next three crops were grown under favorable conditions. Algorithms were developed in Matlab to obtain, over ten image capture sessions, the morphometric and thermal parameters of the roots (perimeter, area, length and average temperature), plants (perimeter, area, height and average temperature), and fruiting. (yield and number of fruits). The statistical analysis was carried out using the ANOVA and Tukey tests considering a value of p ≤ 0.05. The results obtained indicate that there is no significant difference between the characterizations of the four crops. This statement is also supported by the visual analysis of the growth curves parameters of the four crops. In addition, the temperature inside the aeroponic system was contrasted with the ambient temperature and it was verified that the temperature to which the roots are exposed was in the range of 10°C – 20°C. The thermal analysis determined that a plant that presents water stress and is also exposed to high temperatures has an average leaf temperature of 3.7 to 5 °C above the optimal condition for the plant, while a plant with stress at normal temperatures was 1.3 °C higher than the plant without stress.
Why it matches plant phenotyping methodsVIS・NIR・IR画像から根・葉・植物体・果実の形態および温度形質を抽出する手法が研究の中心であり、画像解析アルゴリズムも開発しているため、植物フェノタイピング手法として適格です。
abstractOne of these is the analysis of images captured in the visible and near and far infrared spectrum which involves using non-invasive techniques in order to characterize the growth of a plant and diagnose if it presents any type of stress.
Crop diseases have a terrible impact on food protection and can result in considerable reductions in both the supply and quality of agricultural products. Human professional have traditionally been relying on to diagnose crop diseases caused by insects, pests, virus, bacteria, fungal, inadequate nutrition, or adverse environmental conditions. This, however, is costly, time demanding, and in some situations unworkable. Thus, in the area of agricultural information, the automatic identification of crop diseases is significantly required. Many strategies have been presented to solve this challenge, with deep learning becoming as the preferred approach due to its outstanding performance.This research describes a method for detecting chili leaf diseases using a deep convolutional neural network. We compared performances of four architectures: MobileNet, Inception-ResnetV2, EfficientNetB0, and DenseNet. The proposed approach evaluated the findings using measures such as accuracy, loss and time. Our model compares favorably to EfficientNetB0 with an accuracy of 0.995, a loss of 0.023, and time is 5 minutes 45 seconds. EfficientNetB0, a compact deep learning architecture has fine tuned to classify two forms of chili leaf diseases. The method was tested on 2475 photos from the Plant Village dataset.
Why it matches plant phenotyping methods唐辛子葉の病害状態を画像から推定する深層学習手法が研究の中心であり、複数モデルの性能比較・評価も実施しているため、植物フェノタイピング手法として収録する。
abstractThis research describes a method for detecting chili leaf diseases using a deep convolutional neural network.
With the advancement in modern agricultural technologies, ensuring crop health and enhancing yield have become paramount. This study aims to address potential shortcomings in the existing chili disease detection methods, particularly the absence of optimized model architecture and in-depth domain knowledge integration. By introducing a neural architecture search (NAS) and knowledge graphs, an attempt is made to bridge this gap, targeting enhanced detection accuracy and robustness. A disease detection model based on the Transformer and knowledge graphs is proposed. Upon evaluating various object detection models on edge computing platforms, it was observed that the dynamic head module surpassed the performance of the multi-head attention mechanism during data processing. The experimental results further indicated that when integrating all the data augmentation methods, the model achieved an optimal mean average precision (mAP) of 0.94. Additionally, the dynamic head module exhibited superior accuracy and recall compared to the traditional multi-head attention mechanism. In conclusion, this research offers a novel perspective and methodology for chili disease detection, with aspirations that the findings will contribute to the further advancement of modern agriculture.
Why it matches plant phenotyping methods唐辛子の病徴を画像から検出・分類するモデルの開発と評価が中心であり、植物の病害状態を直接推定するフェノタイピング手法に該当します。
abstractThis study aims to address potential shortcomings in the existing chili disease detection methods, particularly the absence of optimized model architecture and in-depth domain knowledge integration.
Introduction Recently, plant disease detection and diagnosis procedures have become a primary agricultural concern. Early detection of plant diseases enables farmers to take preventative action, stopping the disease's transmission to other plant sections. Plant diseases are a severe hazard to food safety, but because the essential infrastructure is missing in various places around the globe, quick disease diagnosis is still difficult. The plant may experience a variety of attacks, from minor damage to total devastation, depending on how severe the infections are. Thus, early detection of plant diseases is necessary to optimize output to prevent such destruction. The physical examination of plant diseases produced low accuracy, required a lot of time, and could not accurately anticipate the plant disease. Creating an automated method capable of accurately classifying to deal with these issues is vital. Method This research proposes an efficient, novel, and lightweight DeepPlantNet deep learning (DL)-based architecture for predicting and categorizing plant leaf diseases. The proposed DeepPlantNet model comprises 28 learned layers, i.e., 25 convolutional layers (ConV) and three fully connected (FC) layers. The framework employed Leaky RelU (LReLU), batch normalization (BN), fire modules, and a mix of 3×3 and 1×1 filters, making it a novel plant disease classification framework. The Proposed DeepPlantNet model can categorize plant disease images into many classifications. Results The proposed approach categorizes the plant diseases into the following ten groups: Apple_Black_rot (ABR), Cherry_(including_sour)_Powdery_mildew (CPM), Grape_Leaf_blight_(Isariopsis_Leaf_Spot) (GLB), Peach_Bacterial_spot (PBS), Pepper_bell_Bacterial_spot (PBBS), Potato_Early_blight (PEB), Squash_Powdery_mildew (SPM), Strawberry_Leaf_scorch (SLS), bacterial tomato spot (TBS), and maize common rust (MCR). The proposed framework achieved an average accuracy of 98.49 and 99.85in the case of eight-class and three-class classification schemes, respectively. Discussion The experimental findings demonstrated the DeepPlantNet model's superiority to the alternatives. The proposed technique can reduce financial and agricultural output losses by quickly and effectively assisting professionals and farmers in identifying plant leaf diseases.
Why it matches plant phenotyping methods葉画像から植物病害状態を分類する深層学習手法を開発しており、植物の病徴・病害状態の取得と推定が研究の中心であるため。
abstractThis research proposes an efficient, novel, and lightweight DeepPlantNet deep learning (DL)-based architecture for predicting and categorizing plant leaf diseases.
Reproduction assets foundThe paper's plant leaf disease classification experiments are built entirely on two public Kaggle image datasets explicitly cited by the authors: the PlantVillage Dataset (eight-class experiment) and the Plant Disease Prediction Dataset (three-class experiment). No author code, trained model, or supplementary deposit (Dataset · publicWe verified the effectiveness and robustness of the DeepPlantNet model by using images from the publicly available Kaggle “PlantVillage Dataset” dataset ( Dataset : https://www.kaggle.com/datasets/abdallahalidev/plantvillage-dataset ).Open asset ↗Kaggle · abdallahalidev/plantvillage-datasetlines:355-366Dataset · publicWe validated our model using another common, publicly accessible Kaggle dataset, “Plant Disease Prediction Dataset,” to assess and estimate the generalizability and performance of the DeepPlantNet model ( Dataset : https://www.kaggle.com/datasets/shuvranshu/plant-disease-prediction-dataset ).Open asset ↗Kaggle · shuvranshu/plant-disease-prediction-datasetlines:729-756Plant phenotyping relevance match · UnverifiedEurope PMC · checked 15 Sept 2026
Phytophthora capsici is one of the most devastating pathogens facing pepper ( Capsicum annuum ) producers worldwide. Numerous factors, such as the race of the pathogen, the growing environment, and the source of resistance, have resulted in an overall lack of widely applicable molecular markers associated with resistance. Our objective was to determine the effect of the rating system on quantitative trait locus (QTL) detection and understand inheritance patterns of host resistance that can influence selection and molecular marker accuracy. We evaluated an F 2:11 recombinant inbred line population screened against the highly virulent strain (Pc134) and scored using two widely used methods, developed by Bosland and Lindsey and by Black. The rating system developed by Bosland and Lindsey resulted in slightly higher logarithm of odds for the QTL on chromosome 5, and we detected a QTL on chromosome 12 uniquely using this rating system. A QTL on chromosome 10 was detected using both rating systems, but Black resulted in considerably higher logarithm of odds for this QTL compared with the Bosland and Lindsey system. Molecular markers developed were nominally better at accurately predicting the phenotype than previously published molecular markers but did not completely explain resistance in our validation populations. The inheritance pattern of resistance in one of our F 2 populations did not significantly deviate from a 7:9 segregation ratio, indicating duplicative recessive epistasis. However, these results could be confounded by the presence of incomplete gene action, which was found through the improved selection accuracy when the phenotypes of heterozygous individuals were grouped with those with susceptible alleles.
Why it matches plant phenotyping methodsPhytophthora根腐病の植物病徴評価法を比較し、評価システムが表現型判定、QTL検出、選抜精度に与える影響を検証しているため、病害表現型測定法が中心的です。
abstractOur objective was to determine the effect of the rating system on quantitative trait locus (QTL) detection
Agricultural products are vital to the sustainability of the economies of developing countries. Most developing countries' economies such as Ethiopia heavily rely on agriculture. On a global scale, the pepper crop is one of the most important agricultural products in terms of human food security. However, it is susceptible to a variety of diseases which include blight leaf disease, gray leaf spot, common rust, fruit rot disease, powdery mildew symptoms on pepper leaf, and other related diseases that are all common today. Currently, more than 34 different pepper diseases have been discovered, resulting in a 33% average yield loss in pepper cultivation. Conventionally, farmers detect the disease using visual observation but this has its own demerits as it is usually not accurate and usually time consuming. In the past, a number of researchers have presented various methods for classifying pepper plant disease, especially using image processing and deep learning techniques. However, earlier studies have shown that binary classification requires improvement as some classes were more challenging to identify than others. In this study, we propose a concatenated neural network of the extracted features of VGG16 and AlexNet networks to develop a pepper disease classification model using fully connected layers. The development of the proposed concatenated CNN model includes steps such as dataset collection, image preprocessing, noise removal, segmentation, feature extraction, and classification. Finally, the proposed concatenated CNN model was evaluated, providing a training classification accuracy of 100%, validation accuracy of 97.29%, and testing accuracy of 95.82%. In general, it can be concluded from the findings of the study that the proposed concatenated model is suitable for identifying pepper leaf and fruit diseases from digital images of pepper.
Why it matches plant phenotyping methodsコショウ葉・果実の病徴を画像から分類するCNN手法を開発・評価しており、植物の病害状態推定が研究の中心であるため。
abstractwe propose a concatenated neural network of the extracted features of VGG16 and AlexNet networks to develop a pepper disease classification model
Plant diseases pose a significant threat to agricultural productivity and food security in Bangladesh. In this research, we address the challenge of timely and accurate plant disease detection through the application of transfer learning with deep neural models. We curated a diverse dataset comprising 18 categories of plant leaf images, including Bell pepper Bacterial spot, Bell pepper Healthy, Peach Healthy, Potato Early Blight, Rice Leaf Blast, Rice Healthy, Rice Brown Spot, Potato Healthy, Peach Bacterial spot, Corn Blight, Potato Late blight, Corn Healthy, Tomato Bacterial spot, Strawberry Leaf Scorch, Tomato Early blight, Tomato Early blight, Strawberry Healthy, and Tomato Healthy. The dataset represents the most prevalent plant diseases observed in the Bangladeshi context. We employed three state-of-the-art deep learning algorithms, EfficientNetV2M, VGG-19, and NASNetLarge, to develop robust plant disease detection models. Through transfer learning, these pre-trained models were fine-tuned on our specialized dataset to adapt them for the task at hand. The performance evaluation revealed impressive results, with EfficientNetV2M achieving an accuracy rate of 99%, VGG-19 achieving 93%, and NASNetLarge attaining 83% accuracy. The high accuracy of EfficientNetV2M showcases its exceptional capability in accurately classifying plant diseases prevalent in Bangladesh. The success of these deep neural models in detecting various plant diseases signifies their potential in revolutionizing plant disease management and enhancing agricultural practices. Our research contributes valuable insights into the effective use of transfer learning for plant disease detection and emphasizes the significance of dataset curation for improved model performance. The developed models hold promise in providing timely and precise disease diagnosis to farmers and agricultural professionals, thereby facilitating prompt interventions and minimizing crop losses. Future research can explore the integration of these deep neural models into practical agricultural tools, enabling real-time disease detection and offering substantial benefits to the agricultural industry in Bangladesh.
Why it matches plant phenotyping methods植物葉画像から病害状態を推定する深層学習モデルの開発・性能評価が研究の中心であり、植物病害フェノタイピング手法に該当する。
abstractwe address the challenge of timely and accurate plant disease detection through the application of transfer learning with deep neural models.
Pepper leaf disease identification based on convolutional neural networks (CNNs) is one of the interesting research areas. However, most existing CNN-based pepper leaf disease detection models are suboptimal in terms of accuracy and computing performance. In particular, it is challenging to apply CNNs on embedded portable devices due to a large amount of computation and memory consumption for leaf disease recognition in large fields. Therefore, this paper introduces an enhanced lightweight model based on GoogLeNet architecture. The initial step involves compressing the Inception structure to reduce model parameters, leading to a remarkable enhancement in recognition speed. Furthermore, the network incorporates the spatial pyramid pooling structure to seamlessly integrate local and global features. Subsequently, the proposed improved model has been trained on the real dataset of 9183 images, containing 6 types of pepper diseases. The cross-validation results show that the model accuracy is 97.87%, which is 6% higher than that of GoogLeNet based on Inception-V1 and Inception-V3. The memory requirement of the model is only 10.3 MB, which is reduced by 52.31%-86.69%, comparing to GoogLeNet. We have also compared the model with the existing CNN-based models including AlexNet, ResNet-50 and MobileNet-V2. The result shows that the average inference time of the proposed model decreases by 61.49%, 41.78% and 23.81%, respectively. The results show that the proposed enhanced model can significantly improve performance in terms of accuracy and computing efficiency, which has potential to improve productivity in the pepper farming industry.
Why it matches plant phenotyping methodsコショウ葉の病徴を画像から認識するCNNモデルを開発・比較し、精度と計算効率を検証しているため、植物病害状態の画像ベースフェノタイピング手法が中心です。
abstractPepper leaf disease identification based on convolutional neural networks (CNNs) is one of the interesting research areas.
Quality sorting of plug seedlings is an important part of factory nurseries, and deep learning is starting to become a core technology in this field. In response to the key problems of existing sorting systems with a single perspective and insufficient detection indicators, this paper innovatively proposes a lightweight multi-indicator detection model for plug seedlings, and an integrated transient sorting system for high-speed transplanting is designed. The new YOLOv5s model is composed of the ShuffleNet-V2 backbone, a channel attention mechanism neck, and a head block, and it can effectively extract image features of different seedling qualities. We built a dataset for pepper and tomato plug seedlings, and the experimental results showed that the model was highly accurate in detecting no seedlings, weak seedlings, damaged seedlings and strong seedlings, with an mAP of 94.23 %. Finally, in the verification of different operating speeds, the transplanting-sorting-replanting multifunctional system had the best integrated efficiency of 4200 plants per hour, and the average treatment time for single plug seedlings was only 0.86 s. Our method effectively can improve the performance of seedling quality sorting systems and provide technical support for factory nurseries.
Why it matches plant phenotyping methods画像から苗の品質状態を複数指標で推定し、高速選別システムとして検証する手法開発が中心である。
abstractthis paper innovatively proposes a lightweight multi-indicator detection model for plug seedlings, and an integrated transient sorting system for high-speed transplanting is designed
The optimal quality, the marketability and the shelf-life of pepper fruit often depend on the ripening stage at harvest. Moreover, it is necessary to estimate the process of ripening in various steps of the supply chain. The potential of four non-destructive techniques as rapid tools for discriminating the process of ripening was investigated. Chlorophyll fluorescence, Vis/NIR spectroscopy, two commercial digital imaging cameras- one R-G-B dslr and a modified R-G-NIR compact camera were assessed as alternatives to color measurements in classifying pepper fruit according to their ripening stage. Freshly harvested mature bell pepper fruit ‘cv. Denver’ were sorted in six distinct ripening stages (S1-S6), from green to full red (mature green 100% green-S1, green to brown-S2, brown-S3, orange-S4, red-S5 and full red-S6 stage), by visually assessing their color. From the results, it is concluded that the a∗/b∗ color parameter that was determined using a colorimeter was proven to be the most accurate descriptor in ripening stage classification, either at harvest or during storage, as confirmed by chemometrics, such as principal component (PCA), partial least square (PLS) and support vector machine (SVM) analyses and mean comparisons, as well. Therefore, the increase of a∗/b∗ ratio can be used as an indicator to estimate the degree of fruit ripeness. The a∗/b∗ ratio was strongly correlated with fruit spectral reflectance and chlorophyll fluorescence data, as well as with two imaging indices generated by digital R-G-B and R-G-NIR imaging techniques that are reported for the first time on fruit in this study. The above results indicate that it is possible to estimate the a∗/b∗ ratio and simultaneously the ripening stage of pepper fruit with high accuracy and consistency, by using individually several non-destructive techniques. However, freshness status of bell pepper fruit can be reliably assessed, irrespectively of the ripening stage at harvest, only by spectral reflectance data and indeed even by processing only specific wavelengths that were identified after implementing the genetic algorithm.
Why it matches plant phenotyping methodsピーマン果実の成熟度・鮮度という植物器官の状態を、画像、蛍光、分光など複数の非破壊センシングで推定・分類する手法が研究の中心であり、指標生成と精度評価も行っている。
abstractThe potential of four non-destructive techniques as rapid tools for discriminating the process of ripening was investigated.
Automated disease inspection of greenhouse vegetables is less limited by the visibility of heavily infected leaves and more so by the reliable location of early lesions covering only a few dozen pixels. SE-YOLOv8 is a small-object-enhanced detector in this study that maintains a high-resolution P2 path, performs adaptive bidirectional cross-scale fusion, adds a lightweight coordinate attention module, and uses a scale-balanced localization objective. A greenhouse dataset with 12,480 images of tomato, cucumber, pepper and lettuce was divided into five disease categories plus a healthy background, and 31,762 annotated lesions were included. Under a fixed 640 × 640 input, the proposed model achieved 94.8% precision, 92.6% recall, 95.7% , and 72.4% . Relative to YOLOv8s, recall for lesions smaller than 32 × 32 pixels increased by 8.9 percentage points while parameters rose from 11.2 M to 12.7 M. The model sustained 48.6 frames/s on an NVIDIA Jetson Orin NX and reduced the expected calibration error from 6.8% to 3.9%. Ablation and robustness experiments show that the high-resolution branch contributes the most to the gain, and adaptive fusion and coordinate attention improve discrimination under glare, leaf overlap and clutter. Therefore, by maintaining fine spatial information and regulating cross-scale flow of information, early disease localisation can be achieved without reducing the throughput of practical greenhouses.
Why it matches plant phenotyping methods温室作物の病斑を画像から検出・位置推定する深層学習手法を開発し、比較、アブレーション、頑健性、速度、較正を評価しており、植物病害状態の表現型取得が中心である。
abstractSE-YOLOv8 is a small-object-enhanced detector in this study that maintains a high-resolution P2 path, performs adaptive bidirectional cross-scale fusion, adds a lightweight coordinate attention module, and uses a scale-balanced localization objective.
In the era of artificial intelligence, automation is becoming popular in every sector. The primary sector includes the agriculture sector. Farmers are facing problems such as the identification of diseases in their plants, lack of proper treatment for the disease, climatic changes that affect their yield, and low price for their crops. In this paper, we are mainly focusing on the disease identification of bell pepper plants using deep learning architectures such as Alex Net, google net, ResNet (18,50,101), and Vgg (16,19). We also focus on the detailed study of different pre-trained CNN architectures to analyze their performance and identify which architecture is more suitable for disease classification in bell pepper. This paper also helps bell pepper farmers to identify the disease with high accuracy compared to the traditional methods of disease identification. The new automation concept helps bell pepper framers to identify diseases with less time and effort, which makes their work easier. The identification of disease at an early stage with less effort will help the farmer to increase their yield. The paper will help to understand the performance of different pre-trained convolutional neural network architectures with and without augmentation of images and also compare the performance of the architectures. Based on these comparisons, it could find out that google net is more suitable for the classification of images in bell pepper as compared to other architectures with augmentation, and vgg19 was observed to be best for the classification of images without augmentation.
Why it matches plant phenotyping methodsベルペッパー植物の画像から病害を分類するCNN手法を複数比較し、データ拡張の有無を含めて性能評価しているため、植物病害フェノタイピング手法が研究の中心である。
abstractwe are mainly focusing on the disease identification of bell pepper plants using deep learning architectures such as Alex Net, google net, ResNet (18,50,101), and Vgg (16,19).
Reproduction assets foundThe paper's phenotyping input is the public PlantVillage bell pepper (bacterial spot vs. healthy) image dataset obtained from Kaggle, used to train/evaluate the pretrained CNN architectures. No author analysis code, trained models, or supplementary deposits are mentioned.Dataset · publicwe
consider secondary data set which is also known as bench
mark dataset which is available in plant village on Kaggle site.Open asset ↗pdf-page:9 lines:1-45Plant phenotyping relevance match · UnverifiedCrossref · checked 8 Sept 2026
Abstract This work reports a first-of-its-kind hybrid wearable physicochemical sensor suite that we call PlantFit for simultaneous measurement of two key phytohormones, salicylic acid, and ethylene, along with vapor pressure deficit and radial growth of stem in live plants. The sensors are developed using a low-cost and roll-to-roll screen printing technology. A single integrated flexible patch that contains temperature, humidity, salicylic acid, and ethylene sensors, is installed on the leaves of live plants. The strain sensor with in-built pressure correction capability is wrapped around the plant stem to provide pressure-compensated stem diameter measurements. The sensors provide real-time information on plant health under different amounts of water stress conditions. The sensor suite is installed on bell pepper plants for 40 days and measurements of salicylic acid, ethylene, temperature, humidity, and stem diameter are recorded daily. In addition, sensors are installed on different parts of the same plant to investigate the spatiotemporal dynamics of water transport and phytohormone responses. Subsequent correlation and principal component analyses demonstrate the strong association between hormone levels, vapor pressure deficit, and water transport in the plant. Our findings suggest that the mass deployment of PlantFit in agricultural settings will aid growers in detecting water stress/deficiency early and in implementing early intervention measures to reduce stress-induced yield decline.
Why it matches plant phenotyping methods植物の生理状態と成長を連続取得するウェアラブルセンサー群を開発し、実植物で検証・適用しているため、フェノタイピング手法が中心である。
abstractThis work reports a first-of-its-kind hybrid wearable physicochemical sensor suite that we call PlantFit for simultaneous measurement of two key phytohormones, salicylic acid, and ethylene, along with vapor pressure deficit and radial growth of stem in live plants.
Today, the use of sensors and imaging techniques to obtain information about plants and soil in smart irrigation systems is rapidly becoming widespread. This study aimed to investigate the usability of leaf turgor pressure and thermal images to detect water stress and the irrigation time of pepper and to determine their relationship with physiological traits (leaf water potential, stomatal conductivity, superoxide dismutase, and catalase activity). For this purpose, the pepper (Capsicum annuum L. cv. "California Wonder") experiment was carried out in Canakkale, Turkey in 2017 and 2018. A total of four treatments were applied in the experiment, control (100%) and three different water stress levels (75%, 50%, and 25%). Leaf turgor pressure (Pp), thermal images and physiological measurements were carried out during the growing season. Soil moisture and leaf turgor pressure (Pp) were monitored in real time by remote. As a result of the study, the average evapotranspiration (ET c ) was 697 mm, and the yield value was 83.7 t ha − 1 under non-stress conditions. Depending on the decrease in ET c , yield values also decreased significantly. The physiological traits of pepper were also adversely affected by water stress. Leaf water potential and stomatal conductivity values were statistically different in all irrigation treatments. The change in the activity of catalase (CAT) due to water stress was greater than that of superoxide dismutase (SOD). In this case, it can be said that other physiological traits are more successful than SOD in distinguishing water stress. According to the regression models, significant relationships were determined between both the indices calculated from the thermal images and Pp, yield and physiological traits. The predictive ability of Pp values has been strengthened with the addition of meteorological properties to the model in general. The highest correlation (R 2 = 0.63) was between Pp + meteorological properties and the catalase activity. All the regression models between CWSI and I Gp calculated from thermal images were statistically significant. The highest R 2 values were obtained in August. In this month, the highest correlations were between CWSI and leaf water potential and stomatal conductivity (R 2 = 0.91), I Gp and stomatal conductivity (R 2 = 0.80). The predictive power of CWSI p was higher than I Gp . The experiment illustrated that Pp and temperature data, which are plant-based monitoring methods, have the potential to detect water stress in peppers.
Why it matches plant phenotyping methods葉の膨圧と熱画像を用いて pepper の水ストレスや生理状態を推定し、回帰モデルと相関で性能を評価しており、植物表現型取得・推定法が研究の中心である。
abstractThis study aimed to investigate the usability of leaf turgor pressure and thermal images to detect water stress and the irrigation time of pepper
The pollen germination rate decreases under various abiotic stresses, such as high-temperature stress, and it is one of the causes of inhibition of plant reproduction. Thus, measuring pollen germination rate is vital for understanding the reproductive ability of plants. However, measuring the pollen germination rate requires much labor when counting pollen. Therefore, we used the Yolov5 machine learning package in order to perform transfer learning and constructed a model that can detect germinated and non-germinated pollen separately. Pollen images of the chili pepper, Capsicum annuum, were used to create this model. Using images with a width of 640 pixels for training constructed a more accurate model than using images with a width of 320 pixels. This model could estimate the pollen germination rate of the F 2 population of C. chinense previously studied with high accuracy. In addition, significantly associated gene regions previously detected in genome-wide association studies in this F 2 population could again be detected using the pollen germination rate predicted by this model as a trait. Moreover, the model detected rose, tomato, radish, and strawberry pollen grains with similar accuracy to chili pepper. The pollen germination rate could be estimated even for plants other than chili pepper, probably because pollen images were similar among different plant species. We obtained a model that can identify genes related to pollen germination rate through genetic analyses in many plants.
Why it matches plant phenotyping methods機械学習画像解析により、植物花粉の発芽・未発芽を識別し、花粉発芽率という植物生殖形質を自動推定する手法を開発・適用しており、表現型取得が研究の中心である。
abstractwe used the Yolov5 machine learning package in order to perform transfer learning and constructed a model that can detect germinated and non-germinated pollen separately.
Solanaceae pollen cryopreservation is a common practice in the hybrid seed production industry worldwide, enabling effective hybridization across geographical and seasonal limitations. As pollination with low quality pollen can result in significant seed yield loss, monitoring the pollen quality has become an important risk management tool. In this study, pollen quality analysis methods were evaluated for their suitability for routine quality control of cryopreserved pollen batches. The assessments, including pollen viability, pollen germinability and pollen vigor analysis, were conducted in two locations on a diverse set of cryopreserved tomato and pepper pollen batches. While the viability obtained by Impedance Flow Cytometry (IFC) can be interpreted as the pollen's potential to germinate, the in vitro germination assay directly quantifies this functionality under given assay conditions. A linear correlation was found between pollen viability obtained by IFC and in vitro germinability. In conclusion, IFC is the most suitable tool for applications and industries requiring a high degree of automation, throughput, repeatability, and reproducibility. In vitro germination assays are suitable for studies within certain temporal and geographic limitations, due to difficulties in standardization. On the other hand, vigor assessments are not sufficiently addressing the needs of the industry due to poor reproducibility and low throughput.
Why it matches plant phenotyping methods冷凍花粉の品質評価法を比較・検証し、IFCや発芽試験の自動化、再現性、スループットを評価しているため、花粉の生理状態を測定する方法論研究として採用。
abstractIn this study, pollen quality analysis methods were evaluated for their suitability for routine quality control of cryopreserved pollen batches.
Abstract This work reports a first-of-its-kind hybrid wearable physicochemical sensor suite that we call PlantFit for simultaneous measurement of two key phytohormones, salicylic acid, and ethylene, along with vapor pressure deficit and radial growth of stem in live plants. The sensors are developed using a low-cost and roll-to-roll screen printing technology. A single integrated flexible patch that contains temperature, humidity, salicylic acid, and ethylene sensors, is installed on the leaves of live plants. The strain sensor with in-built pressure correction capability is wrapped around the plant stem to provide pressure-compensated stem diameter measurements. The sensors provide real-time information on plant health under different amounts of water stress conditions. The sensor suite is installed on bell pepper plants for 40 days and measurements of salicylic acid, ethylene, temperature, humidity, and stem diameter are recorded daily. In addition, sensors are installed on different parts of the same plant to investigate the spatiotemporal dynamics of water transport and phytohormone responses. Subsequent correlation and principal component analyses demonstrate the strong association between hormone levels, vapor pressure deficit, and water transport in the plant. Our findings suggest that the mass deployment of PlantFit in agricultural settings will aid growers in detecting water stress/deficiency early and in implementing early intervention measures to reduce stress-induced yield decline.
Why it matches plant phenotyping methods生体植物のホルモン、環境状態、茎径を連続測定するウェアラブルセンサー・スイートを開発しており、植物の生理状態・成長形質の取得方法が研究の中心である。
abstractThis work reports a first-of-its-kind hybrid wearable physicochemical sensor suite that we call PlantFit for simultaneous measurement of two key phytohormones, salicylic acid, and ethylene, along with vapor pressure deficit and radial growth of stem in live plants.
In the era of artificial intelligence, deep learning, and computer vision play a vital role in leaf-based disease identification and categorization. Leaf diseases are the most dangerous calamity that has direct detrimental effects on farmers’ lives, and consequently on gross yield production and the world economy. Nutritious food for all is a great challenge faced by the farmer and agricultural research community. Bell peppers can be categorized as fruit or vegetable that is universally available and full of various nutrients like carbs, vitamins, and fat. Leaves of bell pepper plants infected by bacterial spot diseases affect their yield significantly. The aim of this study is to classify bacterial spots and healthy images of bell peppers’ leaf images taken from the PlantVillage dataset using CNN-based pre-trained architecture. Two CNN architectures, i.e., VGG16 and VGG19 are applied through transfer learning in the binary classification of leaf-based disease. A total of 2475 images are used for training, validation, and testing purposes, with 1478 healthy images and 997 images with bacterial disease spots. Although both VGG16 and VGG19 achieved good performances, VGG16 architecture performs slightly better than VGG19.
Why it matches plant phenotyping methodsベルペッパー葉の病斑という植物の病態を画像から分類するCNN手法が研究の中心であり、植物病害フェノタイピングの方法適用に該当する。
abstractThe aim of this study is to classify bacterial spots and healthy images of bell peppers’ leaf images taken from the PlantVillage dataset using CNN-based pre-trained architecture.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Dataset · publicThe leaf images of bell peppers are collected from the PlantVillage datasetOpen asset ↗pdf-page:4 lines:1-41Plant phenotyping relevance match · UnverifiedOpenAlex · Europe PMC · checked 15 Sept 2026
Plant stress responses involve a suite of genetically encoded mechanisms triggered by real-time interactions with their surrounding environment. Although sophisticated regulatory networks maintain proper homeostasis to prevent damage, the tolerance thresholds to these stresses vary significantly among organisms. Current plant phenotyping techniques and observables must be better suited to characterize the real-time metabolic response to stresses. This impedes practical agronomic intervention to avoid irreversible damage and limits our ability to breed improved plant organisms. Here, we introduce a sensitive, wearable electrochemical glucose-selective sensing platform that addresses these problems. Glucose is a primary plant metabolite, a source of energy produced during photosynthesis, and a critical molecular modulator of various cellular processes ranging from germination to senescence. The wearable-like technology integrates a reverse iontophoresis glucose extraction capability with an enzymatic glucose biosensor that offers a sensitivity of 22.7 nA/(μM·cm 2 ), a limit of detection (LOD) of 9.4 μM, and a limit of quantification (LOQ) of 28.5 μM. The system's performance was validated by subjecting three different plant models (sweet pepper, gerbera, and romaine lettuce) to low-light and low-high temperature stresses and demonstrating critical differential physiological responses associated with their glucose metabolism. This technology enables non-invasive, non-destructive, real-time, in-situ, and in-vivo identification of early stress response in plants and provides a unique tool for timely agronomic management of crops and improving breeding strategies based on the dynamics of genome-metabolome-phenome relationships.
Why it matches plant phenotyping methods植物のストレス状態をリアルタイムに推定するウェアラブル電気化学グルコースセンシング基盤を開発し、複数植物種とストレス条件で性能・生理応答を検証しており、表現型取得法が中心である。
abstractHere, we introduce a sensitive, wearable electrochemical glucose-selective sensing platform that addresses these problems.
“Agriculture provides employment opportunities for village people on large scale in developing country like India. Most of Indian farmers are adopting manual cultivation due to lagging of technical knowledge. In addition that, Plant leaf disease has been one of the major threats to for plants since long ago because it reduces the crop yield and compromises. plant diseases are studied in the literature, mostly focusing on the biological aspects. They make predictions according to the visible surface of plants and leaves. This paper presents a system that is used to classify and detect plant leaf diseases using machine learning techniques. In our work, we have taken specific types of plants; include tomatoes, pepper, and potatoes, as they are the most common types of plants in the world and in Iraq in particular. Using machine learning algorithms, which comprise procedures like dataset construction, loading images, prepping, segmentation, feature extraction, training a classifier, and classification, it is possible to classify plant diseases. This paper presents a Convolutional Neural Network (CNN) model algorithm based method for Agricultural leaf disease detection and classification. So, the neural networks can capture the colours and textures of lesions specific to respective diseases upon diagnosis
Why it matches plant phenotyping methodsCNNによる植物葉の病害検出・分類手法が論文の中心であり、葉の可視症状を直接推定する植物フェノタイピング手法に該当する。
abstractThis paper presents a system that is used to classify and detect plant leaf diseases using machine learning techniques.
This study proposed a novel framework for plant leaf disease identification. The proposed model consists of four steps including pre-processing, segmentation, feature extraction, and classification. At first, the unwanted noise and overfitting are removed, and also image contrast level is enhanced. Secondly, the Fuzzy C-Means (FCM) based Chameleon Swarm Algorithm (CSA) named as (FCM-CSA) is used for plant leaf diseased part segmentation. In the third stage, the feature extraction is performed using a fast GLCM feature extraction model. Finally, the Progressive Neural Architecture Search (PNAS) is used for plant leaf disease identification. The experimental investigations are carried out using MATLAB software with the Mendeley database. From this dataset, we have used Apple Cedar Apple Rust (ACAR), Cherry Powdery Mildew (CPM), Corn Common Rust (CCCR), Apple Healthy (AH), Grape Black Rot (GBR), Pepper Bell Bacterial Spot (PBBS), Potato Late Blight (PLB) and Tomato Leaf Mold (TLM) disease images. Different measures such as precision, recall, sensitivity, specificity, and accuracy results are used to validate the performance of the proposed model.
Why it matches plant phenotyping methods植物葉の病変部位を画像から分割し、病害を識別する画像解析ワークフローを開発・検証しており、植物状態(病害)の推定手法が中心である。
abstractThe proposed model consists of four steps including pre-processing, segmentation, feature extraction, and classification.
The segmentation of pepper leaves from pepper images is of great significance for the accurate control of pepper leaf diseases. To address the issue, we propose a bidirectional attention fusion network combing the convolution neural network (CNN) and Swin Transformer, called BAF-Net, to segment the pepper leaf image. Specially, BAF-Net first uses a multi-scale fusion feature (MSFF) branch to extract the long-range dependencies by constructing the cascaded Swin Transformer-based and CNN-based block, which is based on the U-shape architecture. Then, it uses a full-scale feature fusion (FSFF) branch to enhance the boundary information and attain the detailed information. Finally, an adaptive bidirectional attention module is designed to bridge the relation of the MSFF and FSFF features. The results on four pepper leaf datasets demonstrated that our model obtains F1 scores of 96.75%, 91.10%, 97.34% and 94.42%, and IoU of 95.68%, 86.76%, 96.12% and 91.44%, respectively. Compared to the state-of-the-art models, the proposed model achieves better segmentation performance. The code will be available at the website: https://github.com/fangchj2002/BAF-Net.
Why it matches plant phenotyping methodsペッパー葉画像から葉領域を抽出する画像解析手法を開発・検証しており、植物器官の形態状態取得が研究の中心である。
abstractwe propose a bidirectional attention fusion network combing the convolution neural network (CNN) and Swin Transformer, called BAF-Net, to segment the pepper leaf image.
Reproduction assets foundThe paper's authors state that the BAF-Net segmentation code will be available at their GitHub URL (https://github.com/fangchj2002/BAF-Net), which is a paper-specific analysis code asset. The pepper leaf image dataset itself has no public deposit statement, and LabelMe is a generic third-party annotation tool, not a论文-Code · publicprovided the original author(s) and the The code will be available at the website: https://github.com/fangchj2002/Open asset ↗fangchj2002pdf-page:1 lines:1-79Plant phenotyping relevance match · UnverifiedEurope PMC · checked 15 Sept 2026
The optical filter is critical in many applications requiring wide-angle imaging perception. However, the transmission curve of the typical optical filter will change at an oblique incident angle due to the optical path of the incident light change. In this study, we propose a wide-angular tolerance optical filter design method based on the transfer matrix method and automatic differentiation. A novel optical merit function is proposed for simultaneous optimization at normal and oblique incidents. The simulation results demonstrate that such a wide-angular tolerance design can realize a similar transmittance curve at an oblique incident angle compared to a normal incident angle. Furthermore, how much improvement in a wide-angular optical filter design for oblique incident contributes to image segmentation remains unclear. Therefore, we evaluate several transmittance curves along with the U-Net structure for green pepper segmentation. Although our proposed method is not perfectly equal to the target design, it can achieve an average 50% smaller mean absolute error (MAE) than the original design at 20∘ oblique incident angle. In addition, the green pepper segmentation results show that wide-angular tolerance optical filter design improves the segmentation of the near-color object about 0.3% at 20∘ oblique incident angle compared to the previous design.
Why it matches plant phenotyping methods広角耐性光学フィルタと画像分割を開発・評価し、緑ピーマンという植物器官の画像取得・抽出性能を検証しているため、植物フェノタイピング手法が中心です。
abstractTherefore, we evaluate several transmittance curves along with the U-Net structure for green pepper segmentation.
Agriculture improvement is a global economic issue and ongoing challenge in this covid-19 pandemic that is highly dependent on effectiveness. The recognition of the diseases in plant leaf performs a major role in the agriculture industry and city-side greenhouse farms approximate analysis of the leaf disease this article intends to integrate image processing techniques with the “convolutional neural network”, which is one of the deep learning approaches, to classify and detect plant leaf disease and publicly available plant the late data to help treat the leaf as early as possible, which controls the economic loss. This paper has a set that was used which consists of 10 classes of disease and three classes of a plant leaf, this research offers an effective method for detecting different diseases in plant leaf variations. The model was created to detect and recognize healthy plant kinds, such as tomato and potato, and pepper these three leaves will perform under the algorithm called a convolutional neural network. By modifying the parameters and changing the pulling combination, models that have been used to train and test these types of leaf sample images can be created. leaf disease recognition was based on these 10 different types of classes in three different species tomato, potato, and pepper the classification of sample images has reached diseases identification accuracy.
Why it matches plant phenotyping methods植物葉の画像から病害状態をCNNで分類・検出する手法が研究の中心であり、植物病害フェノタイピングに該当する。
abstractthis article intends to integrate image processing techniques with the “convolutional neural network”, which is one of the deep learning approaches, to classify and detect plant leaf disease
Stereo matching is a depth perception method for plant phenotyping with high throughput. In recent years, the accuracy and real-time performance of the stereo matching models have been greatly improved. While the training process relies on specialized large-scale datasets, in this research, we aim to address the issue in building stereo matching datasets. A semi-automatic method was proposed to acquire the ground truth, including camera calibration, image registration, and disparity image generation. On the basis of this method, spinach, tomato, pepper, and pumpkin were considered for experiment, and a dataset named PlantStereo was built for reconstruction. Taking data size, disparity accuracy, disparity density, and data type into consideration, PlantStereo outperforms other representative stereo matching datasets. Experimental results showed that, compared with the disparity accuracy at pixel level, the disparity accuracy at sub-pixel level can remarkably improve the matching accuracy. More specifically, for PSMNet, the EPE and bad−3 error decreased 0.30 pixels and 2.13%, respectively. For GwcNet, the EPE and bad−3 error decreased 0.08 pixels and 0.42%, respectively. In addition, the proposed workflow based on stereo matching can achieve competitive results compared with other depth perception methods, such as Time-of-Flight (ToF) and structured light, when considering depth error (2.5 mm at 0.7 m), real-time performance (50 fps at 1046 × 606), and cost. The proposed method can be adopted to build stereo matching datasets, and the workflow can be used for depth perception in plant phenotyping.
Why it matches plant phenotyping methods植物フェノタイピング用のステレオマッチングデータセット構築法を開発し、精度・性能を検証した研究であり、表現型取得手法が中心である。
abstractStereo matching is a depth perception method for plant phenotyping with high throughput.
Plant-induced electrical signals (PIES) can be non-destructively monitored by inserting electrodes into plant stems, which reflect plant nutrient and water uptake. The main objective of this study was to evaluate the growth of pepper plants with different urea applications (low fertilizer: N0, Control: N1, and high fertilizer: N2) in soil by monitoring PIES. The PIES value was found to be low in the low urea treatment group while the two times higher urea applied pepper had the highest PIES value. The nutritional content of the stem, leaves and soil did not correlate with PIES because of dilution effect by high biomass with high urea application, but principal component analysis showed that the PIES was positively associated with pepper biomass and soil EC. The high fertilizer did not affect chlorophyll and proline contents in pepper leaves. The assessment of plant growth by PIES has advantages because non-destructive, real time and remote monitoring is possible. Therefore, PIES monitoring of different plants grown under various cultivation environments is useful method to evaluate plant activity and growth.
Why it matches plant phenotyping methods植物の成長・活動を非破壊かつリアルタイムに推定するPIES測定が研究の中心であり、肥料試験への応用と植物フェノタイプ評価法としての有用性を扱っている。
abstractPlant-induced electrical signals (PIES) can be non-destructively monitored by inserting electrodes into plant stems, which reflect plant nutrient and water uptake.
Plants are the primary source of food for world's population. Diseases in plants can cause yield loss, which can be mitigated by continual monitoring. Monitoring plant diseases manually is difficult and prone to errors. Using computer vision and artificial intelligence (AI) for the early identification of plant illnesses can prevent the negative consequences of diseases at the very beginning and overcome the limitations of continuous manual monitoring. The research focuses on the development of an automatic system capable of performing the segmentation of leaf lesions and the detection of disease without requiring human intervention. To get lesion region segmentation, we propose a context-aware 3D Convolutional Neural Network (CNN) model based on CANet architecture that considers the ambiguity of plant lesion placement in the plant leaf image subregions. A Deep CNN is employed to recognize the subtype of leaf lesion using the segmented lesion area. Finally, the plant's survival is predicted using a hybrid method combining CNN and Linear Regression. To evaluate the efficacy and effectiveness of our proposed plant disease detection scheme and survival prediction, we utilized the Plant Village Benchmark Dataset, which is composed of several photos of plant leaves affected by a certain disease. Using the DICE and IoU matrices, the segmentation model performance for plant leaf lesion segmentation is evaluated. The proposed lesion segmentation model achieved an average accuracy of 92% with an IoU of 90%. In comparison, the lesion subtype recognition model achieves accuracies of 91.11%, 93.01 and 99.04 for pepper, potato and tomato plants. The higher accuracy of the proposed model indicates that it can be utilized for real-time disease detection in unmanned aerial vehicles and offline to offer crop health updates and reduce the risk of low yield.
Why it matches plant phenotyping methods植物葉の病斑領域を画像から分割・認識し、病害状態と生存確率を推定する手法の開発・評価が研究の中心であるため。
abstractThe research focuses on the development of an automatic system capable of performing the segmentation of leaf lesions and the detection of disease without requiring human intervention.
Traditional machine learning methods of plant leaf disease detection lack successful performances due to poor feature representation and correlation. This paper presents a novel methodology for automatic plant leaf disease detection using cascaded deep convolutional neural network (CDCNN) which focusses on increasing the feature representation and correlation factors. It provides distinctive features that gives low intra-class variability and higher inter-class variability. CDCNN were performed on a plant-village leaf disease database which consists of 13 classes of tomato, potato, and pepper bell plant diseases; DCNN model performs better with an overall accuracy, recall, and precision of 98.50%, 0.98, and 0.97 respectively. Additionally, performance of the proposed algorithm is evaluated on real time cotton leaf database for bacterial blight, leaf miner, and spider mite diseases detection and provides 99.00% accuracy. The proposed DCNN outperforms well compared to traditional machine learning and deep learning models and is able to detect the diseases present in the leaves of the plant.
Why it matches plant phenotyping methods植物葉の病害状態を画像から推定する深層学習手法を開発・比較評価しており、植物フェノタイピング手法が中心である。
abstractThis paper presents a novel methodology for automatic plant leaf disease detection using cascaded deep convolutional neural network (CDCNN)
The Iberian Peninsula was the place where pepper (Capsicum annuum) entered Europe and dispersed to other continents but was also an important secondary center for its diversification. The current work evaluated the phenotypic diversity existing in this region and investigated how that evolved from Capsicum native areas (Mexico and Andean Region). For that purpose, the high-throughput phenotyping tool Tomato Analyzer was employed. Descriptors related to size and shape were the most distinctive among fruit types, reflecting a broad diversity for Iberian peppers. These traits likely reflected those suffering from more intensive human selections, driving the worldwide expansion of C. annuum. Iberian peppers maintained close proximity to the American accessions in terms of fruit phenomics. The highest similarities were observed for those coming from the southeastern edge of the Peninsula, while northwestern accessions displayed more significant differences. Common fruit traits (small, conical) suggested that Portuguese and Spanish landraces may have arisen from an ancient American population that entered the south of Spain and promptly migrated to the central and northern territories, giving rise to larger, elongated, and blocky pods. Such lineages would be the result of adaptations to local soil–climate factors prevailing in different biogeographic provinces.
Why it matches plant phenotyping methodsTomato Analyzerを用いた高スループット表現型解析が、ペッパー果実のサイズ・形状多様性を評価する研究の中心であり、方法の実質的な適用に該当する。
abstractFor that purpose, the high-throughput phenotyping tool Tomato Analyzer was employed.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Supplement · publicThe following supporting information can be downloaded at https://www.mdpi.com/article/10.3390/plants11223075/s1 , Table S1. Analysis of variance for conventional (FWE and FLP) and Tomato Analyzer descriptors among Mexico, Andean Region, Iberian Peninsula, and Mediterranean Basin; Table S2. Analysis of variance for conventional (FWE and FLP) and Tomato Analyzer descriptors among Iberian biogeographic provinces; Table S3. Analysis of variance for conventional and Tomato Analyzer descriptors between pungent and non-pungent peppers. Only the significantly different parameters are shown. FEW = fruit weight, FPL = fruit pedicel length; see Table S5 for acronyms of TA descriptors; Table S4. ScoresOpen asset ↗lines:424-438Code / dataset availability confirmedCrossref · checked 13 Sept 2026
Abstract Plant disease detection has a huge impact on plant farming. Early diagnosis of plant illness can help control disease spread and reduce loss. It is a soil-borne disease that affects leaves of plants. In the current research, the emphasis is on the early diagnosis and prevention of plant leaf disease. In this paper, strawberry, tomato, pepper bell, and potato disease detection network (STPP- DDN) based on Faster R-CNN and multi-task learning. STPP-ddn is developed which leverages attention mechanisms in feature extraction. STPP-DDN detects disease based on plant symptoms. Unlike other approaches for diagnosing disease from the total plant look, the STPP-DDN automatically classifies the petioles and young leaves. A large dataset with number of photos divided into various groups is constructed to serve as a basis for analyzing and testing our proposed technique. Each image also includes a label that indicates whether or not the plants has been affected. With the proposed STPP-DDN, we achieved a mAP of 77:54% on object detection of 4 categories and 99:95% accuracy for strawberry verticillium wilt detection.
Why it matches plant phenotyping methods植物病徴を画像から検出・分類する深層学習手法を開発し、データセットと精度評価を提示しており、植物の疾病状態の表現型取得が中心です。撤回表示はあるものの、内容はスクリーニング対象に該当します。
abstractSTPP-DDN detects disease based on plant symptoms.
Reproduction assets foundThe withdrawn preprint states that the plant leaf image datasets used for its disease-detection experiments (potato, tomato, pepper bell, and strawberry) are freely available on open-source platforms, with explicit public URLs given in footnotes: a Kaggle plant disease dataset and a GitHub strawberry verticillium wilt.Dataset · publicthese datasets are freely available on open source plat- forms; potato, tomato, pepper bell 1 strawberry 2 .Open asset ↗lines:103-113Plant phenotyping relevance match · UnverifiedEurope PMC · checked 15 Sept 2026
Published1 Nov 2022Computers and Electronics in Agriculture.
Pepper / chilliField / plotRootMorphology / geometry measurementSegmentationRoot system architecture
In situ measurement of root traits is very essential for better understanding of root development. However, difficulty in acquisition of root traits, fine roots and root hairs in particular, from low contrast background presents challenges for root traits segmentation. In this paper, an in-ground device termed microrhizotron was used for local root area observation and capture. An efficient and accurate model was proposed for segmenting local detailed root (fine roots and root hairs) image by constructing region of interest and adding prior knowledge in convolutional neural network. In order to reduce complexity of root image, regional growth result was used to position the root area and thus construct the region of interest. Transfer learning was applied to pre-train models on relevant dataset as initial parameters. Root axes and root hairs were separated using a pruning method, and root hair parameters were extracted based on it. The result showed that pre-trained features on relevant dataset could somewhat overcome small dataset, and prior knowledge reduced mislearning of background. The P-T-U-Net model (U-Net base on prior knowledge and transfer learning) had the best performance in root segmentation among all the applied models. The Intersection over Union (IoU), Pixel accuracy (PA), and F1 was 0.869, 0.977, and 0.872 respectively for pepper root hairs. It succeed in root traits segmentation with an average F1 score over 0.9. Plant species has little influence on the segmentation. It was able to figure out details on crossing and overlapping roots, and was able to focus on local root areas over time.
Why it matches plant phenotyping methods根毛・細根画像から植物形質を抽出する画像処理手法を開発し、セグメンテーション性能を検証しているため、植物フェノタイピング手法が中心です。
abstractAn efficient and accurate model was proposed for segmenting local detailed root (fine roots and root hairs) image by constructing region of interest and adding prior knowledge in convolutional neural network.
American Spice Trade Association (ASTA) color value is a key factor for determining the commercial value of red pepper. This study investigates the potential of predicting ASTA color values of red pepper using LED-induced fluorescence. Excitation emission matrixes (EEMs) were first obtained in a front-face configuration. To determine the excitation wavelengths for LED-induced fluorescence measurement, parallel factor analysis (PARAFAC) combined with EEMs were used. According to PARAFAC, 375 nm was selected be the peak wavelength of the excitation light source of the LED-induced fluorescence system. The spectra obtained from the system were used to predict ASTA color values using partial least squares regression (PLSR) and support vector machine (SVM). With optimal preprocessing method, the highest correlation coefficients of prediction (Rₚ) could reach 0.971 with RMSEP of 3.672, RPD of 3.563 and REP of 6.170. Results revealed that LED-induced fluorescence has the potential for rapid prediction of ASTA color values of red peppers.
Why it matches plant phenotyping methodsLED誘導蛍光と回帰モデルにより、赤ピーマンの色値という果実形質を予測する測定法の開発・評価が中心であり、単なる生物学的実験のルーチン測定ではない。
abstractThis study investigates the potential of predicting ASTA color values of red pepper using LED-induced fluorescence.
As food is an essential element in life, modern possibilities must be harnessed to pay attention to it. In this paper, we will discuss the discovery of early plant diseases classification using artificial intelligence technology, we made in this study analysis of convolutional neural networks architecture (vgg-16, mobile net, efficient net) and made a comparison between these model in accuracy and loos data in each model, we used data set from the Kaggle site that contain 20640 picture from different disease of plant (potato, tomato and pepper) this pictures divided on 15 class but unbalanced. at the first we solved the problem of train the models with multi class, we made balanced data and training the work in environment of Google Colab, we used it in train three models vgg-16, mobile net, efficient net, which showed this study that the accuracy of work in efficient net is 98% more than other models and loss data in this model is less than other models.
Why it matches plant phenotyping methods植物葉の病害状態を画像から分類するCNN手法を比較・評価しており、病害表現型の抽出とモデル性能比較が研究の中心です。
titlePerformance Comparison of Convolutional Neural Network Models for Plant Leaf Disease Classification
Crop fresh weight and leaf area are considered non-destructive growth factors due to their direct relation to vegetative growth and carbon assimilation. Several methods to measure these parameters have been introduced; however, measuring these parameters using the existing methods can be difficult. Therefore, a non-destructive measurement method with high versatility is essential. The objective of this study was to establish a non-destructive monitoring system for estimating the fresh weight and leaf area of trellised crops. The data were collected from a greenhouse with sweet peppers ( Capsicum annuum var. annuum ); the target growth factors were the crop fresh weight and leaf area. The crop fresh weight was estimated based on the total system weight and volumetric water content using a simple formula. The leaf area was estimated using top-view images of the crops and a convolutional neural network (ConvNet). The estimated crop fresh weight and leaf area exhibited average R 2 values of 0.70 and 0.95, respectively. The simple calculation was able to avoid overfitting with fewer limitations compared with the previous study. ConvNet was able to analyze raw images and evaluate the leaf area without additional sensors and features. As the simple calculation and ConvNet could adequately estimate the target growth factors, the monitoring system can be used for data collection in practice owing to its versatility. Therefore, the proposed monitoring system can be widely applied for diverse data analyses.
Why it matches plant phenotyping methods作物の生体重と葉面積を非破壊推定する計測システムの開発が中心であり、画像とCNNによる形質抽出および検証を行っている。
abstractTherefore, a non-destructive measurement method with high versatility is essential.
Reproduction assets foundThe paper's Supplementary Materials (hosted by MDPI at the allowed URL) explicitly contain sample crop images used as phenotyping inputs, trained-model validation results, model architectures, training parameters, and leaf-area regression coefficients — directly reproducing this paper's fresh-weight and leaf-area phenySupplement · publicowth can be found in the raw data containing changes in the image and weight. Therefore, a monitoring system that can collect both factors can be widely applied for data analyses, such as machine learning, crop modeling, and data standardization.
Supplementary Materials
The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/s22207728/s1 , Figure S1: Sample images collected from the camera. Images were cropped and resized into 128 × 128, and the resized images were augmented using flipping and shifting; Figure S2: Validation accuracies of the trained deep learning models for estimating the calculated fresh weight.; Figure S3: Validation accuracy of theOpen asset ↗lines:129-147Plant phenotyping relevance match · UnverifiedEurope PMC · Crossref · checked 8 Sept 2026
In the recent past, the agricultural industry has rapidly digitalized in the form of smart farms through the broad usage of data analysis and artificial intelligence. Commonly, high operating costs in a smart farm are primarily due to inefficient energy usage. Therefore, accurate estimation of agricultural energy usage and environmental factors is considered as one of the significant tasks for crop growth control. The growth sequences of crops in agricultural environments like smart farms are related to agricultural energy usage and consumption. This study aims to develop and validate an algorithm that can interpret the crop growth rate response to environmental and solar energy factors based on machine learning, and to evaluate the algorithm's accuracy compared to the base model. The proposed model was determined through a comparative experiment of three representative machine learning techniques, which are random forest (RF), support vector machine (SVM), and gradient boosting machine (GBM), considering the energy usage for environmental control is highly associated with the paprika crop growth. Through the experiment performance with real data gathered from a paprika smart farm in South Korea, the multi-level RF can effectively predict paprika growth with an accuracy of 0.88, considering data analysis of factors that use solar energy. As a result of the experiment with the suggested model, the growth factors such as leaf length, leaf width, and environmental factors were found. Furthermore, the proposed algorithm can contribute to the development of applications through analysis of the crop growth big data for various plants in agricultural environments such as a smart farm.
Why it matches plant phenotyping methods機械学習によりパプリカの成長(葉長・葉幅)を推定するアルゴリズムを開発・検証しており、植物形質の抽出が中心的な方法論的貢献である。
abstractThis study aims to develop and validate an algorithm that can interpret the crop growth rate response to environmental and solar energy factors based on machine learning, and to evaluate the algorithm's accuracy compared to the base model.
The emergence of deep neural networks has allowed the development of fully automated and efficient diagnostic systems for plant disease and pest phenotyping. Although previous approaches have proven to be promising, they are limited, especially in real-life scenarios, to properly diagnose and characterize the problem. In this work, we propose a framework which besides recognizing and localizing various plant abnormalities also informs the user about the severity of the diseases infecting the plant. By taking a single image as input, our algorithm is able to generate detailed descriptive phrases (user-defined) that display the location, severity stage, and visual attributes of all the abnormalities that are present in the image. Our framework is composed of three main components. One of them is a detector that accurately and efficiently recognizes and localizes the abnormalities in plants by extracting region-based anomaly features using a deep neural network-based feature extractor. The second one is an encoder-decoder network that performs pixel-level analysis to generate abnormality-specific severity levels. Lastly is an integration unit which aggregates the information of these units and assigns unique IDs to all the detected anomaly instances, thus generating descriptive sentences describing the location, severity, and class of anomalies infecting plants. We discuss two possible ways of utilizing the abovementioned units in a single framework. We evaluate and analyze the efficacy of both approaches on newly constructed diverse paprika disease and pest recognition datasets, comprising six anomaly categories along with 11 different severity levels. Our algorithm achieves mean average precision of 91.7% for the abnormality detection task and a mean panoptic quality score of 70.78% for severity level prediction. Our algorithm provides a practical and cost-efficient solution to farmers that facilitates proper handling of crops.
Why it matches plant phenotyping methods画像から植物病害・害虫の位置と重症度を推定する深層学習システムの開発・評価が研究の中心であり、植物の病害状態を直接定量化するため、植物フェノタイピング手法として含める。
abstractwe propose a framework which besides recognizing and localizing various plant abnormalities also informs the user about the severity of the diseases infecting the plant.
Reproduction assets foundThe paper's data availability statement points to the authors' public GitHub repository (DIANA) hosting the raw paprika disease/pest dataset supporting the phenotyping analysis.Dataset · publicThe raw data supporting the conclusions of this article will be made available by the authors at following link: https://github.com/Mr-TalhaIlyas/DIANA .Open asset ↗Mr-TalhaIlyas/DIANAlines:1128-1209Plant phenotyping relevance match · UnverifiedarXiv · OpenAlex · checked 13 Sept 2026
Active perception for fruit mapping and harvesting is a difficult task since occlusions occur frequently and the location as well as size of fruits change over time. State-of-the-art viewpoint planning approaches utilize computationally expensive ray casting operations to find good viewpoints aiming at maximizing information gain and covering the fruits in the scene. In this paper, we present a novel viewpoint planning approach that explicitly uses information about the predicted fruit shapes to compute targeted viewpoints that observe as yet unobserved parts of the fruits. Furthermore, we formulate the concept of viewpoint dissimilarity to reduce the sampling space for more efficient selection of useful, dissimilar viewpoints. Our simulation experiments with a UR5e arm equipped with an RGB-D sensor provide a quantitative demonstration of the efficacy of our iterative next best view planning method based on shape completion. In comparative experiments with a state-of-the-art viewpoint planner, we demonstrate improvement not only in the estimation of the fruit sizes, but also in their reconstruction, while significantly reducing the planning time. Finally, we show the viability of our approach for mapping sweet peppers plants with a real robotic system in a commercial glasshouse.
Why it matches plant phenotyping methods果実形状の再構成とサイズ推定を目的とする視点計画法を開発・比較評価しており、植物表現型取得が中心的な技術貢献である。
abstractwe present a novel viewpoint planning approach that explicitly uses information about the predicted fruit shapes to compute targeted viewpoints that observe as yet unobserved parts of the fruits.
The biggest threat to the safety of food is plant diseases. They have the ability to dramatically lower the quantity and quality of agricultural products. Recognizing plant diseases is the biggest issue in the agricultural industries. Convolutional Neural Networks (CNN) are effective in solving image classification problems in computer vision. Numerous deep learning architectures have been used to diagnose plant diseases. This study presents a transfer learning-based model for identifying diseases in plant leaves. In this paper, a CNN classifier based on transfer learning model called DenseNet201 are proposed. An analysis of four deep learning models (VGG16, Inception V3, ResNet152V2, and DenseNet201) done to see which one can detect plant diseases with the greatest degree of accuracy. Web based application developed for plant disease diagnosing from defected leaf image and the proposed model which identify the disease and give the recommended treatment. The used images dataset contains 28310 leaves photos of 3 crops, tomato, potato and pepper divided into 15 different classes, 9 disorders and one healthy class for tomato, 2 disorders and one healthy class for potato and 1 disorder and one healthy for pepper. In our experimental, the results shows that the proposed model achieves the highest training accuracy of 99.44% and validation accuracy of 98.70%.
Why it matches plant phenotyping methods葉画像から植物病害を診断するCNNモデルとWebアプリを開発・比較しており、植物の病害状態を画像から推定する方法が研究の中心である。
abstractThis study presents a transfer learning-based model for identifying diseases in plant leaves.
The recent advancement of big data technology causes the data from agriculture domain to enter into the big data. They are not conventional techniques in existence to process such a large volume of data. The processing of large datasets involves parallel computation and analysis model. Hence, it is necessary to use big data analytics framework to process a large image datasets. In this paper, an automated big data framework is presented to classify the plant disease condition. This framework consists of a series operations that leads into a final step. When the classification is carried out using novel image classifier. The image classifier is designed using a Convolutional Recurrent Neural Network Classifier (CRNN) algorithm. The classifier is designed in such a way that it provides classification between a normal leaf and an abnormal leaf. The classification of plant images over large datasets that includes banana plant, pepper, potato, and tomato plant. Which is compared with other existing big data plant classification techniques like convolutional neural network, recurrent neural network, and deep neural network, artificial neural network with forward and backward propagation. The result shows that the proposed method obtains improved detection and classification of diseased plants compared to other the convolutional neural network (94.14%), recurrent neural network (94.07%), deep neural network (94%), artificial neural network with forward (93.96%), and backward propagation method (93.66%).
Why it matches plant phenotyping methods植物画像から病害状態を分類するCRNNベースの画像解析フレームワークを開発・比較しており、病害状態という植物表現型の抽出が中心である。
abstractan automated big data framework is presented to classify the plant disease condition
The classification of plug seedling quality plays an active role in enhancing the quality of seedlings. The EfficientNet-B7-CBAM model, an improved convolutional neural network (CNN) model, was proposed to improve classification efficiency and reduce high cost. To ensure that the EfficientNet-B7 model simultaneously learns crucial channel and spatial location information, the convolutional block attention module (CBAM) has been incorporated. To improve the model's ability to generalize, a transfer learning strategy and Adam optimization algorithm were introduced. A system for image acquisition collected 8,109 images of pepper plug seedlings, and data augmentation techniques improved the resulting data set. The proposed EfficientNet-B7-CBAM model achieved an average accuracy of 97.99% on the test set, 7.32% higher than before the improvement. Under the same experimental conditions, the classification accuracy increased by 8.88-20.05% to classical network models such as AlexNet, VGG16, InceptionV3, ResNet50, and DenseNet121. The proposed method had high accuracy in the plug seedling quality classification task. It was well-adapted to numerous types of plug seedlings, providing a reference for developing a fast and accurate algorithm for plug seedling quality classification.
Why it matches plant phenotyping methods画像からトウガラシ苗の品質状態を分類するCNN手法と画像取得システムが研究の中心であり、植物表現型の推定に該当する。
abstractThe EfficientNet-B7-CBAM model, an improved convolutional neural network (CNN) model, was proposed to improve classification efficiency and reduce high cost.
Abstract Visible traits can be criteria for selecting a suitable crop. Three-dimensional (3D)-scanned plant models can be used to extract visible traits; however, collecting scanned data and physically manipulating point-cloud structures of the scanned models are difficult. Recently, deep generative models have shown high performance in learning and creating target data. Deep generative models can improve the versatility of scanned models. The objectives of this study were to generate sweet pepper (Capsicum annuum) leaf models and to extract their traits by using deep generative models. The leaves were scanned, preprocessed and used to train the deep generative models. The variational autoencoder, generative adversarial network (GAN) and latent space GAN were used to generate the desired leaves. The optimal number of latent variables in the model was selected via the Jensen–Shannon divergence (JSD). The generated leaves were evaluated by using the JSD, coverage and minimum matching distance to determine the best model for leaf generation. Among the deep generative models, a modified GAN showed the highest performance. Sweet pepper leaves with various shapes were generated from eight latent variables following a normal distribution, and the morphological traits of the leaves were controlled through linear interpolation and simple arithmetic operations in latent space. Simple arithmetic operations and gradual changes in the latent space modified the leaf traits. Deep generative models can parameterize and generate morphological traits in digitized 3D plant models and add realism and diversity to plant phenotyping studies.
Why it matches plant phenotyping methods3Dスキャン葉から形態形質を抽出・生成する深層生成モデルを開発・評価しており、植物フェノタイピング手法が研究の中心である。
abstractThe objectives of this study were to generate sweet pepper (Capsicum annuum) leaf models and to extract their traits by using deep generative models.
We designed and built a homemade computed tomography imaging spectrometer (CTIS) of 250×250 p i x e l s of spatial resolution and 2 nm spectral resolution. The optical design considers a CTIS optical array coupled to a digital reflex camera. We reconstructed the intensity spectra of a fluorescent source, the diffuse reflectance of a ColorChecker, and samples of Capsicum annuum of three different colors, using the expectation-maximization sequential algorithm, optimized utilizing an array of indices to reduce the reconstruction time. The results obtained with a ColorChecker indicate a high positive correlation of 0.9745 with an average residual difference of 1.31% concerning the spectra obtained with a commercial integrating sphere spectrometer. The feasibility of the proposed CTIS system shows how to detect and evaluate the physiological changes resulting from the decomposition of the green fruit of the Capsicum annuum in a range from 500 to 650 nm.
Why it matches plant phenotyping methods植物の生理状態を評価するCTIS分光イメージングシステムを設計・構築し、再構成アルゴリズムと商用分光計との性能比較を行っており、植物フェノタイピング手法が中心である。
abstractWe designed and built a homemade computed tomography imaging spectrometer (CTIS)
In agriculture, the majority of vision systems perform still image classification. Yet, recent work has highlighted the potential of spatial and temporal cues as a rich source of information to improve the classification performance. In this paper, we propose novel approaches to explicitly capture both spatial and temporal information to improve the classification of deep convolutional neural networks. We leverage available RGB-D images and robot odometry to perform inter-frame feature map spatial registration. This information is then fused within recurrent deep learnt models, to improve their accuracy and robustness. We demonstrate that this can considerably improve the classification performance with our best performing spatial-temporal model (ST-Atte) achieving absolute performance improvements for intersection-over-union (IoU[%]) of 4.7 for crop-weed segmentation and 2.6 for fruit (sweet pepper) segmentation. Furthermore, we show that these approaches are robust to variable framerates and odometry errors, which are frequently observed in real-world applications.
Why it matches plant phenotyping methodsRGB-D画像とロボットオドメトリを用いて空間・時間情報を統合する画像解析手法を開発し、作物・果実のセグメンテーション性能を検証しているため、植物表現型取得手法が中心である。
abstractWe demonstrate that this can considerably improve the classification performance with our best performing spatial-temporal model (ST-Atte) achieving absolute performance improvements for intersection-over-union (IoU[%]) of 4.7 for crop-weed segmentation and 2.6 for fruit (sweet pepper) segmentation.
Agriculture is the backbone of world’s economy. This sector faces predominant issues in recognizing crop infection, disease prediction, pest control, weed detection and yield prediction leading to the shortfall in both quality and production of food. To ensure food safety, high resilience and increased crop yields, the precise diagnosis and recognition of underlying plant disease along with classification of crops from weeds is vital. The recent advancements in automatic feature extraction and classification techniques using Artificial Intelligence have gained attraction in the field of agriculture and crop protection. This paper proposes a Novel Convolutional Neural Network model for crop disease classification. The model is trained and tested in publicly available Plant Village Dataset with 38 categories and 15 classes. For the experimental analysis, the model is trained with 5 classes which includes potato and pepper bell categories. Further, the performance of the proposed model is analyzed with machine leaning models such as Support Vector Machine (SVM), K-Nearest Neighborhood (K-NN), Random Forest, Decision Tree and have attained the highest accuracy of 91.28%. In the testing phase, it is observed that this model is superior in terms of accuracy, specificity, precision, recall and F1-Score.
Why it matches plant phenotyping methods植物画像から病害状態を分類するCNN手法の開発・性能比較が中心であり、植物病害フェノタイピングに該当する。
abstractThis paper proposes a Novel Convolutional Neural Network model for crop disease classification.
Common beanPepper / chilliField / plotSegmentation
Fine segmentation labelling tasks are time consuming and typically require a great deal of manual labor. This paper presents a novel method for efficiently creating pixel-level fine segmentation labelling that significantly reduces the amount of necessary human labor. The proposed method utilizes easily produced multiple and complementary coarse labels to build a complete fine label via supervised learning. The primary label among the coarse labels is the manual label, which is produced with simple contours or bounding boxes that roughly encompass an object. All others coarse labels are complementary and are generated automatically using existing algorithms. Fine labels can be rapidly created during the supervised learning of such coarse labels. In the experimental study, the proposed technique achieved a fine label IOU (intersection of union) of 92% in segmenting our newly constructed bean field dataset. The proposed method also achieved 95% and 92% mean IOU when tested on publicly available agricultural CVPPP and CWFID datasets, respectively. Our proposed method of segmentation also achieved a mean IOU of 81% when it was tested on our newly constructed paprika disease dataset, which includes multiple categories.
Why it matches plant phenotyping methods画素レベル画像セグメンテーション手法そのものを開発し、植物・農業およびパプリカ病害データセットで性能評価しているため、植物画像から状態・領域を抽出する方法研究として中心的です。
abstractThis paper presents a novel method for efficiently creating pixel-level fine segmentation labelling
Reproduction assets foundThe paper's authors explicitly state their analysis code is publicly available on GitHub. The newly constructed plant datasets (Bean-Field, Paprika-Disease, Circle) are only available on request, so they qualify as request_only, not public.Code · publicCode availability
The code is available at https://github.com/hololee/coarse-to-fine-segmentation-labelling .Open asset ↗hololee/coarse-to-fine-segmentation-labellinglines:226-263Plant phenotyping relevance match · UnverifiedEurope PMC · checked 14 Sept 2026
Published4 May 2022Spectrochimica acta. Part A, Molecular and biomolecular spectroscopyCited by 51 · OpenAlex ↗
Pre-processing is a crucial step in analyzing spectra from Fourier transform infrared (FTIR) spectroscopy because it can reduce unwanted noise and enhance system performance. Here, we present the results of pre-processing technique optimization to facilitate the detection of pepper yellow leaf curl virus (PYLCV)-infected chilli plants using FTIR spectroscopy. Optimization of a range of pre-processing techniques was undertaken, namely baseline correction, normalization (standard normal variate, vector, and min-max), and de-noising (Savitzky-Golay (SG) smoothing, 1st and 2 derivatives). The pre-processing was applied to the mid-infrared spectral range (4000 - 400 cm -1 ) and the biofingerprint region (1800 - 900 cm -1 ) then the discrete wavelet transform (DWT) was used for dimension reduction. The pre-processed data were then used as an input for classification using a multilayer perceptron neural network, a support vector machine, and linear discriminant analysis. The pre-processing method with the highest classification model accuracy was selected for the further use in the processing. It was seen that only the SG 1st derivative method applied to both wavenumber ranges could produce 100% accuracy. This result was supported by principal component analysis clustering. Thus, we have demonstrated that by using the right pre-processing technique, classification success can be increased, and the process simplified by optimization and minimization of the technique used.
Why it matches plant phenotyping methodsFTIRスペクトルの前処理最適化と分類精度比較が中心で、感染植物の病害状態を推定するフェノタイピング手法を技術的に評価している。
abstractPre-processing is a crucial step in analyzing spectra from Fourier transform infrared (FTIR) spectroscopy because it can reduce unwanted noise and enhance system performance.
Plant phenotyping relevance match · UnverifiedOpenAlex · Crossref · Europe PMC · checked 8 Sept 2026
BACKGROUND: There is a demand for non-destructive systems in plant phenotyping which could precisely measure plant traits for growth monitoring. In this study, the growth of chilli plants (Capsicum annum L.) was monitored in outdoor conditions. A non-destructive solution is proposed for growth monitoring in 3D using a single mobile phone camera based on a structure from motion algorithm. A method to measure leaf length and leaf width when the leaf is curled is also proposed. Various plant traits such as number of leaves, stem height, leaf length, and leaf width were measured from the reconstructed and segmented 3D models at different plant growth stages. RESULTS: The accuracy of the proposed system is measured by comparing the values derived from the 3D plant model with manual measurements. The results demonstrate that the proposed system has potential to non-destructively monitor plant growth in outdoor conditions with high precision, when compared to the state-of-the-art systems. CONCLUSIONS: In conclusion, this study demonstrated that the methods proposed to calculate plant traits can monitor plant growth in outdoor conditions.
Why it matches plant phenotyping methods3D画像再構成・セグメンテーションにより植物形質を推定し、手測定と比較検証しており、フェノタイピング手法が研究の中心です。
abstractA non-destructive solution is proposed for growth monitoring in 3D using a single mobile phone camera based on a structure from motion algorithm.
Proximal remote sensing devices are novel tools that enable the study of plant health status through the measurement of specific characteristics, including the color or spectrum of light reflected or transmitted by the leaves or the canopy. The aim of this study is to compare the RGB and multispectral data collected during five years (2016–2020) of four fruiting vegetables (melon, tomato, eggplant, and peppers) with trial treatments of non-grafted and grafted onto resistant rootstocks cultivated in a Meloidogyne incognita (a root-knot nematode) infested soil in a greenhouse. The proximal remote sensing of plant health status data collected was divided into three levels. Firstly, leaf level pigments were measured using two different handheld sensors (SPAD and Dualex). Secondly, canopy vigor and biomass were assessed using vegetation indices derived from RGB images and the Normalized Difference Vegetation Index (NDVI) measured with a portable spectroradiometer (Greenseeker). Third, we assessed plant level water stress, as a consequence of the root damage by nematodes, using stomatal conductance measured with a porometer and indirectly using plant temperature with an infrared thermometer, and also the stable carbon isotope composition of leaf dry matter.. It was found that the interaction between treatments and crops (ANOVA) was statistically different for only four of seventeen parameters: flavonoid (p
Why it matches plant phenotyping methods植物の健康状態を複数の近接リモートセンシング機器で測定・比較し、葉・群落・個体レベルの形質抽出を技術的に評価しているため、手法の実質的応用に該当します。
abstractProximal remote sensing devices are novel tools that enable the study of plant health status through the measurement of specific characteristics
Plants play an essential role in the life of any living organism, human or animal, so protecting this organism from disease is an urgent necessity for the survival of living organisms. The development of science has reduced the time required to discover a disease, allowing us to detect and treat diseases early using artificial intelligence. In this scientific paper, we compared the performance of (Vgg-16, MobileNet, and ConvNext) through time and accuracy among these models. We trained these models by using transfer learning models of convolutional neural networks (CNN) to classify plant diseases (potatoes, tomatoes, and peppers). We used a dataset containing 20,639 images divided into 15 classes of different diseases, we also used the same number of parameter and the same number of layers in vgg16 and mobilenet but in convnext 24 layers.The dataset from the Kaggle site and the work environment on google colab using Python language. The results are shown vgg-16 is a high accuracy of 0.97 during the training process from convnext and mobilenet,but MobilNet is faster in the time 62s.
Why it matches plant phenotyping methods植物葉画像から病害状態を分類するCNN手法を比較・評価しており、病害表現型の取得・推定が研究の中心である。
abstractwe compared the performance of (Vgg-16, MobileNet, and ConvNext) through time and accuracy among these models.
As possible diseases develop on plant leaves, classification is constantly hampered by obstacles such as overfitting and low accuracy. To distinguish healthy products from defective ones, the agricultural industry requires precise and error-free analysis. Deep convolutional neural networks are an efficient model of autonomous feature extraction that has been shown to be fairly effective for detection and classification tasks. However, deep convolutional neural networks often require a large amount of training data, cannot be translated, and need a number of parameters to be specified and tweaked. This paper proposes a highly effective structure that can be applied to classifying multiple leaf diseases of plants and fruits during the feature extraction step. It uses a deep transfer learning model that has been modified to serve this purpose. In summary, we use model engineering (ME) to extract features. Multiple support vector machine (SVM) models are employed to enhance feature discrimination and processing speed. The kernel parameters of the radial basis function (RBF) are determined based on the selected model in the training step. PlantVillage and UCI datasets were used to analyze six leaf image sets containing healthy and diseased leaves of apple, corn, cotton, grape, pepper, and rice. The classification process resulted in approximately 90,000 images. During the experimental implementation phase, the results show the potential of a powerful model in classification operations, which will be beneficial for a variety of future leaf disease diagnostic applications for the agricultural industry.
Why it matches plant phenotyping methods植物葉の画像から病害状態を分類する深層学習・SVM手法の開発と評価が中心であり、植物病害フェノタイピングに該当する。
abstractThis paper proposes a highly effective structure that can be applied to classifying multiple leaf diseases of plants and fruits during the feature extraction step.
The selection of effective and representative spectral bands is extremely important in eliminating redundant information and reducing the computational burden for the potential real-time applications of hyperspectral imaging. However, current band selection methods act as a separate procedure before model training and are implemented merely based on extracted average spectra without incorporating spatial information. In this paper, an end-to-end trainable network framework that combines band selection, feature extraction, and model training was proposed based on a 3D CNN (convolutional neural network, CNN) with the attention mechanism embedded in its first layer. The learned band attention vector was adopted as the basis of a band importance indicator to select effective bands. The proposed network was evaluated by two datasets, a regression dataset for predicting the relative chlorophyll content (soil and plant analyzer development, SPAD) of basil leaves and a classification dataset for detecting the drought stress of pepper leaves. A number of calibration models, including SVM, 1D-CNN, 2B-CNN (two-branch CNN), 3D ResNet and the developed network were established for performance comparison. Results showed that the effective bands selected by the proposed attention-based model achieved higher regression R 2 values and classification accuracies not only than the full-spectrum data, but also than the comparative band selection methods, including traditional SPA (successive projections algorithm) and GA (genetic algorithm) methods and the latest 2B-CNN algorithm. In addition, different from the traditional methods, the proposed band selection algorithm can effectively select bands while carrying out model training and can simultaneously take advantage of the original spectral-spatial information. The results confirmed the usefulness of the proposed attention mechanism-based convolutional network for selecting the most effective band combination of hyperspectral images.
Why it matches plant phenotyping methods植物のSPAD推定と乾燥ストレス検出を対象に、ハイパースペクトル画像のバンド選択と特徴抽出を統合したCNN手法を開発し、複数手法・データセットで性能比較しているため、フェノタイピング手法が中心である。
abstractan end-to-end trainable network framework that combines band selection, feature extraction, and model training was proposed based on a 3D CNN (convolutional neural network, CNN) with the attention mechanism embedded in its first layer.
Inspired by recent promising results in sim-to-real transfer in deep learning we built a realistic simulation environment combining a Robot Operating System (ROS)-compatible physics simulator (Gazebo) with Cycles, the realistic production rendering engine from Blender. The proposed simulator pipeline allows us to simulate near-realistic RGB-D images. To showcase the capabilities of the simulator pipeline we propose a case study that focuses on indoor robotic farming. We developed a solution for sweet pepper yield estimation task. Our approach to yield estimation starts with aerial robotics control and trajectory planning, combined with deep learning-based pepper detection, and a clustering approach for counting fruit. The results of this case study show that we can combine real time dynamic simulation with near realistic rendering capabilities to simulate complex robotic systems.
Why it matches plant phenotyping methods屋内農業向けにRGB-D画像シミュレーション基盤と、コショウ果実の検出・計数による収量推定手法を開発しており、植物形質取得・推定が中心的である。
abstractThe proposed simulator pipeline allows us to simulate near-realistic RGB-D images.
Plant disease classification using deep learning techniques is a popular research area due to the numerous opportunities for introducing advance and robust classifiers. Nevertheless, classifying chilli plant diseases accurately from images under uncontrolled environment and various imaging conditions remains unsolved due to the lack of chilli disease image datasets. In this study, the efficacy of three high-performance deep learning algorithms, namely VGG16, InceptionV3, and EfficientNetB0, in classifying three types of chilli leaves diseases, namely upward curling, mosaic/mottling, and the bacterial spot, is demonstrated. These methods are popularly used for other plant disease classifications due to their effectiveness. The experiments were performed on the 3,000 chilli plant disease images collected from three different field environments in Selangor, Malaysia. The images were captured with a complex background and various illuminations, angles, and distances to reflect the real-life scenarios. The complexity of the collected images was created based on the taxonomic information of chilli leaves diseases and the unavailability of chilli disease images under various imaging conditions in the publicly available plant disease databases. Experimented using appropriate specifications, the models demonstrated outstanding performance with more than 95% accuracy with the highest accuracy of 98.83% by InceptionV3.
Why it matches plant phenotyping methods唐辛子葉の画像から病害状態を深層学習で分類する手法を開発・比較し、異なる野外画像条件で性能評価しているため、植物フェノタイピング手法が中心です。
abstractPlant disease classification using deep learning techniques is a popular research area
This work focuses on the problem of non-contact measurement for vegetables in agricultural automation. The application of computer vision in assisted agricultural production significantly improves work efficiency due to the rapid development of information technology and artificial intelligence. Based on object detection and stereo cameras, this paper proposes an intelligent method for vegetable recognition and size estimation. The method obtains colorful images and depth maps with a binocular stereo camera. Then detection networks classify four kinds of common vegetables (cucumber, eggplant, tomato and pepper) and locate six points for each object. Finally, the size of vegetables is calculated using the pixel position and depth of keypoints. Experimental results show that the proposed method can classify four kinds of common vegetables within 60 cm and accurately estimate their diameter and length. The work provides an innovative idea for solving the vegetable's non-contact measurement problems and can promote the application of computer vision in agricultural automation.
Why it matches plant phenotyping methods野菜の長さ・直径という植物器官形質を、ステレオカメラ、深度画像、キーポイント検出で非接触推定する手法が研究の中心であるため。
abstractThis work focuses on the problem of non-contact measurement for vegetables in agricultural automation.
Reproduction assets foundThe authors publicly released both the vegetable keypoint dataset (1600 COCO-format images with ROI boxes and six keypoints) and the implementation code for their size estimation method on GitHub. Labelme is a generic third-party annotation tool and is excluded.Code · publicThe implementation code of our size estimation method can be accessed on https://github.com/BourneZ130/VegetableDetection , accessed on 15 February 2022.Open asset ↗BourneZ130/VegetableDetectionlines:76-141Plant phenotyping relevance match · UnverifiedEurope PMC · Crossref · checked 8 Sept 2026
To increase nitrogen (N) use efficiency and reduce water pollution from vegetable production, it is necessary to optimize N management. Fluorescence-based optical sensors are devices that can improve N fertilization through non-destructive field monitoring of crop variables. The aim of this work was to compare the performance of five fluorescence indices (SFR-R, SFR-G, FLAV, NBI-R, and NBI-G) to predict crop variables, as dry matter production, crop N content, crop N uptake, Nitrogen Nutrition Index (NNI), absolute and relative yield, in sweet pepper (Capsicum annuum) crops grown in greenhouse. Fluorescence measurements were periodically made with the Multiplex® 3.6 sensor throughout three cropping cycles subjected to five N application treatments. The performance of fluorescence indices to predict crop variables considered calibration and validation analyses. In general, the five fluorescence indices were strongly related with NNI, crop N content and relative yield. The best performing indices to predict crop N content and NNI at the early stages of the crops (i.e., vegetative and flowering phenological stages) were the SFR indices, both under red (SFR-R) and green (SFR-G) excitation. However, in the final stage of the crop (i.e., harvest stage), the best performing indices were NBI, both under red (NBI-R) and green (NBI-G) excitation, and FLAV. The two SFR indices best predicted relative yield of sweet pepper at early growth stages. Overall, the fluorescence sensor and the fluorescence indices evaluated were able to predict crop variables related to N status in sweet pepper. They have the capacity to be incorporated into best N management practices.
Why it matches plant phenotyping methods蛍光センサーと指標による作物N状態・収量関連形質の予測性能を校正・検証しており、植物フェノタイピング手法が中心である。
abstractThe aim of this work was to compare the performance of five fluorescence indices (SFR-R, SFR-G, FLAV, NBI-R, and NBI-G) to predict crop variables
There has been no study on using near-infrared spectroscopy (NIRS) to predict the hotness of fresh pepper. This study is aimed at developing a non-destructive and accurate method for determining the hotness of fresh peppers using portable NIRS and the variable selection strategy. Spectra from different locations on samples were obtained non-destructively with a single scan. Quantitative models were established using partial least squares (PLS) with a variable selection method or fusion method. The results showed that near-stalk was the best spectral acquisition location for quantitative analysis. The variable selection strategy allows the selection of targeted characteristic variables and improves the results. A fusion method, namely variable adaptive boosting partial least squares (VABPLS), was selected for optimal prediction of the performance. In the optimized model, the root mean square errors of prediction for the validation set (RMSEPvs) of capsaicin, dihydrocapsaicin and pungency degree were 0.295, 0.143 and 47.770, respectively, while the root mean square errors of prediction for the prediction set (RMSEPps) collected one month later were 0.273, 0.346 and 75.524, respectively.
Why it matches plant phenotyping methods携帯型NIRSと変数選択を用いて、生鮮トウガラシ果実の辛味(カプサイシン等)を非破壊推定する測定法の開発が中心であり、植物器官の形質・状態を定量化している。
abstractThis study is aimed at developing a non-destructive and accurate method for determining the hotness of fresh peppers using portable NIRS and the variable selection strategy.
Abstract Reliable phenotyping methods that are simple to operate and inexpensive to deploy are critical for studying quantitative traits in plants. Traditional fruit shape phenotyping relies on human raters or 2D analyses to assess form, e.g., size and shape. Systems for 3D imaging using multi‐view stereo have been implemented, but frequently rely on commercial software and/or specialized hardware, which can lead to limitations in accessibility and scalability. We present a complete system constructed of consumer‐grade components for capturing, calibrating, and reconstructing the 3D form of small‐to‐moderate sized fruits and tubers. Data acquisition and image capture sessions are 9 seconds to capture 60 images. The initial prototype cost was $1600 USD. We measured accuracy by comparing reconstructed models of 3D printed ground truth objects to the original digital files of those same ground truth objects. The R 2 between length of the primary, secondary, and tertiary axes, volume, and surface area of the ground‐truth object and the reconstructed models was >0.97 and root‐mean square error (RMSE) was 0.99). Qualitative assessments were performed on 48 fruit and tubers, including 18 strawberries, 12 potatoes, five grapes, seven peppers, and four Bosc and two red Anjou pears. Our proposed phenotyping system is fast, relatively low cost, and has demonstrated accuracy for certain shape classes, and could be used for the 3D analysis of fruit form.
Why it matches plant phenotyping methods果実形状の3D取得・再構成システムを開発し、基準物体との比較で精度検証しているため、植物表現型取得法が中心である。
abstractWe present a complete system constructed of consumer‐grade components for capturing, calibrating, and reconstructing the 3D form of small‐to‐moderate sized fruits and tubers.
Pepper / chilliRootMorphology / geometry measurementRoot system architecture
Capsicum genus comprised of several cultivars is considered as an important spice crop worldwide. Roots play a vital role in a plant to mine water from the deeper layers of the soil. Although, characterisation for root traits have been made using different containers in many crops, such efforts for phenotyping root characteristics in Capsicum species are limited. Therefore, the experiment was initiated to find out the influence of container size on root characteristics and also to identify the appropriate container for high throughput phenotyping of Capsicum species for desirable root characteristics. Nine genotypes belonging to different Capsicum spp. were grown in three types of containers having different dimensions. Among the three types of containers, the bucket type container with dimension of 32 cm height 30 cm diameter with 23 kg soil media capacity was most suitable for phenotyping root characteristics compared to PVC pipe and pot type. Subsequently, 18 genotypes were phenotyped for plant growth and root characteristics in the bucket type container. The genotypes IHR 4517, IHR 3529, IHR 4501, IHR 4550, IHR 4491 and IHR 3241 with better root characteristics were identified.
Why it matches plant phenotyping methods根系形態のハイスループット表現型解析に適した容器を比較・選定し、根特性測定系を技術的に評価しているため、表現型取得法が中心である。
abstractefforts for phenotyping root characteristics in Capsicum species are limited
Plant phenotyping relevance match · UnverifiedCrossref · Europe PMC · checked 8 Sept 2026
Farmers require diverse and complex information to make agronomical decisions about crop management including intervention tasks. Generally, this information is gathered by farmers traversing their fields or glasshouses which is often a time consuming and potentially expensive process. In recent years, robotic platforms have gained significant traction due to advances in artificial intelligence. However, these platforms are usually tied to one setting (such as arable farmland), or algorithms are designed for a single platform. This creates a significant gap between available technology and farmer requirements. We propose a novel field agnostic monitoring technique that is able to operate on two different robots, in arable farmland or a glasshouse (horticultural setting). Instance segmentation forms the backbone of this approach from which object location and class, object area, and yield information can be obtained. In arable farmland, our segmentation network is able to estimate crop and weed at a species level and in a glasshouse we are able to estimate the sweet pepper and their ripeness. For yield information, we introduce a novel matching criterion that removes the pixel-wise constraints of previous versions. This approach is able to accurately estimate the number of fruit (sweet pepper) in a glasshouse with a normalized absolute error of 4.7% and an R 2 of 0.901 with the visual ground truth. When applied to cluttered arable farmland scenes it improves on the prior approach by 50%. Finally, a qualitative analysis shows the validity of this agnostic monitoring algorithm by supplying decision enabling information to the farmer such as the impact of a low level weeding intervention scheme.
Why it matches plant phenotyping methods複数ロボット・環境に対応する画像セグメンテーション手法を開発し、果実数、面積、成熟度などの植物形質を推定して精度評価しているため、フェノタイピング手法が中心である。
abstractWe propose a novel field agnostic monitoring technique that is able to operate on two different robots, in arable farmland or a glasshouse (horticultural setting).
Past studies of plant disease and pest recognition used classification methods that presented a singular recognition result to the user. Unfortunately, incorrect recognition results may be output, which may lead to further crop damage. To address this issue, there is a need for a system that suggest several candidate results and allow the user to make the final decision. In this study, we propose a method for diagnosing plant diseases and identifying pests using deep features based on transfer learning. To extract deep features, we employ pre-trained VGG and ResNet 50 architectures based on the ImageNet dataset, and output disease and pest images similar to a query image via a k -nearest-neighbor algorithm. In this study, we use a total of 23,868 images of 19 types of hot-pepper diseases and pests, for which, the proposed model achieves accuracies of 96.02 and 99.61%, respectively. We also measure the effects of fine-tuning and distance metrics. The results show that the use of fine-tuning-based deep features increases accuracy by approximately 0.7-7.38%, and the Bray-Curtis distance achieves an accuracy of approximately 0.65-1.51% higher than the Euclidean distance.
Why it matches plant phenotyping methods植物画像から病害状態・害虫被害を診断する画像解析手法を提案し、転移学習、微調整、距離指標の効果と精度を評価しており、フェノタイピング手法が中心です。
abstractwe propose a method for diagnosing plant diseases and identifying pests using deep features based on transfer learning.
The internal structure of a seed plays a vital role during germination. Hence, before planting the seed internal quality inspection is advantageous to produce healthy seedlings. In this study, an X-ray CT scanner was used to generate CT images of five-year-old naturally aged pepper seeds. Several processing techniques such as reslicing, feature extraction, and classification were performed on these images. The reslicing process was applied to construct three different planes, namely, transaxial, sagittal, and coronal plane images from raw CT images. Then, three images were selected from each sample (one from each plane) for feature extraction. Using a pattern recognition algorithm, the gray-level co-occurrence matrix (GLCM) was created for each image, and twenty-two types of statistical derivations were performed to generate GLCM textural features. A supervised data matrix was constructed based on the germination results of the seed samples from the images, where the seeds were divided into two classes: normal viable seeds (class-1) and nonviable & abnormal viable seeds (class-2). Supervised classification methods, such as partial least-squares discriminant analysis (PLS-DA), support vector machine (SVM), and K-nearest neighbor (KNN) were used to evaluate the best outcome. Among the tested classifiers, PLS-DA provided the highest accuracy of 88.7% with five-fold cross validation, where seven important features extracted from different angles (θ) were found from the beta coefficient to be significant in the classification of the seed. Results from this study show that X-ray CT imaging incorporated with a pattern recognition system is a robust technique to classify seeds based on their internal quality attributes.
Why it matches plant phenotyping methodsX線CT画像と画像特徴抽出・分類を組み合わせ、種子の生存性・異常性という植物状態を推定する手法が研究の中心であり、交差検証による技術評価も行っている。
abstractSeveral processing techniques such as reslicing, feature extraction, and classification were performed on these images.
In response to the difficulty of plant leaf disease detection and classification, this study proposes a novel plant leaf disease detection method called deep block attention SSD (DBA_SSD) for disease identification and disease degree classification of plant leaves. We propose three plant leaf detection methods, namely, squeeze-and-excitation SSD (Se_SSD), deep block SSD (DB_SSD), and DBA_SSD. Se_SSD fuses SSD feature extraction network and attention mechanism channel, DB_SSD improves VGG feature extraction network, and DBA_SSD fuses the improved VGG network and channel attention mechanism. To reduce the training time and accelerate the training process, the convolutional layers trained in the Image Net image dataset by the VGG model are migrated to this model, whereas the collected plant leaves disease image dataset is randomly divided into training set, validation set, and test set in the ratio of 8:1:1. We chose the PlantVillage dataset after careful consideration because it contains images related to the domain of interest. This dataset consists of images of 14 plants, including images of apples, tomatoes, strawberries, peppers, and potatoes, as well as the leaves of other plants. In addition, data enhancement methods, such as histogram equalization and horizontal flip were used to expand the image data. The performance of the three improved algorithms is compared and analyzed in the same environment and with the classical target detection algorithms YOLOv4, YOLOv3, Faster RCNN, and YOLOv4 tiny. Experiments show that DBA_SSD outperforms the two other improved algorithms, and its performance in comparative analysis is superior to other target detection algorithms.
Why it matches plant phenotyping methods植物葉画像から病害の同定と病害度を推定する深層学習画像解析手法を開発し、複数手法および既存アルゴリズムと性能比較しているため、植物フェノタイピング手法が中心である。
abstractthis study proposes a novel plant leaf disease detection method called deep block attention SSD (DBA_SSD) for disease identification and disease degree classification of plant leaves.
Beyond the use of 2D images, the analysis of 3D images is also necessary for analyzing the phenomics of crop plants. In this study, we configured a system and implemented an algorithm for the 3D image reconstruction of red pepper plant (Capsicum annuum L.), as well as its automatic analysis. A Kinect v2 with a depth sensor and a high-resolution RGB camera were used to obtain more accurate reconstructed 3D images. The reconstructed 3D images were compared with conventional reconstructed images, and the data of the reconstructed images were analyzed with respect to their directly measured features and accuracy, such as leaf number, width, and plant height. Several algorithms for image extraction and segmentation were applied for automatic analysis. The results showed that the proposed method showed an error of about 5 mm or less when reconstructing and analyzing 3D images, and was suitable for phenotypic analysis. The images and analysis algorithms obtained by the 3D reconstruction method are expected to be applied to various image processing studies.
Why it matches plant phenotyping methods植物の3D画像再構成と自動形質抽出システムの開発が中心であり、葉数・葉幅・草丈を対象に精度評価も行っているため、植物フェノタイピング手法として明確に該当する。
abstractwe configured a system and implemented an algorithm for the 3D image reconstruction of red pepper plant (Capsicum annuum L.), as well as its automatic analysis.
Photosynthesis in host plants is significantly reduced by many virus families. The early detection of viral infection before the onset of visual symptoms in both directly and systemically infected leaves is critical in crop protection. Viral pathogens cause a variety of symptoms through modifications of chloroplast structure and function and the response of the photochemistry process is immediate. Therefore, chlorophyll fluorescence monitoring has been extensively investigated the last two decades as a tool for timely assessment of pathogenic threats. Alternatively, the analysis of Chla fluorescence transients offers several interlinked parameters which describe the fate of excitation energy round and through the photosystems. Additionally, OJIP fluorescence transients and leaf reflectance spectra methodologies serve for rapid screening of large number of samples. The objective of the present study was to achieve early detection of viral infection, integrating the multiparametric information of the Chla fluorescence transients and of the leaf reflectance spectra into one photochemical performance index. Infection decreased the maximum quantum yield of PSII (F V /F M ), the effective quantum yield of PSII (Φ PSII ), the CO 2 assimilation rate (A) and the stomatal conductance (g s ) in the studied TMV-pepper plant pathosystem, while non-photochemical quenching (NPQ) increased. Some parameters from the OJIP transients and the leaf reflectance spectra were significantly affected 24 h after infection, while others modified three to five days later. Similar results were obtained from systemically infected leaves but with one to three days hysteresis compared to inoculated leaves. Differences between healthy and infected leaves were marginal during the first 24 h post infection. The Integrated Biomarker Response tool was used to create a photochemical infection index (PINFI) which integrates the partial effects of infection on each fluorescence and reflectance index. The PINFI, which to the best of our knowledge is the first photochemical infection index created by the IBR method, discriminated reliably between the infected and healthy leaves of pepper plants from the first 24 h after infection with the TMV.
Why it matches plant phenotyping methods蛍光・反射スペクトルを統合した植物感染状態の推定指標(PINFI)を開発し、TMV感染 pepper 葉で早期検出性能を評価しており、表現型取得・抽出法が中心である。
abstractThe objective of the present study was to achieve early detection of viral infection, integrating the multiparametric information of the Chla fluorescence transients and of the leaf reflectance spectra into one photochemical performance index.
Plant phenotyping relevance match · UnverifiedOpenAlex · bioRxiv · Europe PMC · checked 15 Sept 2026
Reliable phenotyping methods that are simple to operate and inexpensive to deploy are critical for studying quantitative traits in plants. Traditional fruit shape phenotyping relies on human raters or 2D analyses to assess form, e.g., size and shape. Systems for 3D imaging using multi-view stereo have been implemented, but frequently rely on commercial software and/or specialized hardware, which can lead to limitations in accessibility and scalability. We present a complete system constructed of consumer-grade components for capturing, calibrating, and reconstructing the 3D form of small-to-moderate sized fruits and tubers. Data acquisition and image capture sessions are 9 seconds to capture 60 images. The initial prototype cost was $1600 USD. We measured accuracy by comparing reconstructed models of 3D printed ground truth objects to the original digital files of those same ground truth objects. The R 2 between length of the primary, secondary, and tertiary axes, volume, and surface area of the ground-truth object and the reconstructed models was > 0.97 and root-mean square error (RMSE) was 0.99). Qualitative assessments were performed on 48 fruit and tubers, including 18 strawberries, 12 potatoes, 5 grapes, 7 peppers, and 4 Bosc and 2 red Anjou pears. Our proposed phenotyping system is fast, relatively low cost, and has demonstrated accuracy for certain shape classes, and could be used for the 3D analysis of fruit form.
Why it matches plant phenotyping methods果実・塊茎の3D形状を取得・再構成する低コスト高スループット画像フェノタイピングシステムの開発と精度検証が研究の中心である。
abstractWe present a complete system constructed of consumer-grade components for capturing, calibrating, and reconstructing the 3D form of small-to-moderate sized fruits and tubers.
An ensemble model of convolutional neural network (CNN) and multilayer perceptron (MLP) models was developed to detect sweet pepper (Capsicum annuum var. annuum) fruits in images and predict their development stages. The plants were grown in four rows in a greenhouse, and images were collected from each row. Plant environment and growth data were collected every minute and month, respectively. The fruit development stage was classified into immature, breaking, and mature stages with a CNN using images. The immature stage was internally divided into four stages with an MLP, so a total of six stages were classified using the CNN–MLP ensemble model. The plant growth and environmental data and the information from the CNN output were used for the MLP input. The average accuracy of the six stages was F1 score = 0.77 and IoU = 0.86. The ensemble model showed acceptable performance in predicting fruit development stages. The CNN-only model could classify the mature and breaking stages well, but the immature stages were not distinguished, while the MLP-only model could hardly classify the fruit stage except the immature stages. The most influential factors in classification were the data obtained from CNN and the plant growth and environment data. The ensemble models could help in appropriate labour allocation and strategic management by detecting individual fruits in images and predicting precise fruit development stages.
Why it matches plant phenotyping methods画像から個々の果実を検出し、CNN–MLPにより果実の発育段階という植物器官の状態を推定する手法を開発・評価しており、フェノタイピング手法が中心である。
abstractAn ensemble model of convolutional neural network (CNN) and multilayer perceptron (MLP) models was developed to detect sweet pepper (Capsicum annuum var. annuum) fruits in images and predict their development stages.
There is a growing demand for developing image sensor systems to aid fruit and vegetable harvesting, and crop growth prediction in precision agriculture. In this paper, we present an end-to-end optimization approach for the simultaneous design of optical filters and green pepper segmentation neural networks. Our optimization method modeled the optical filter as one learnable neural network layer and attached it to the subsequent camera spectral response (CSR) layer and segmentation neural network for green pepper segmentation. We used not only the standard red-green-blue output from the CSR layer but also the color-ratio maps as additional cues in the visible wavelength and to augment the feature maps as the input for segmentation. We evaluated how well our proposed color-ratio maps enhanced optical filter design methods in our collected dataset. We find that our proposed method can yield a better performance than both an optical filter RGB system without color-ratio maps and a raw RGB camera (without an optical filter) system. The proposed learning-based framework can potentially build better image sensor systems for green pepper segmentation.
Why it matches plant phenotyping methods緑ピーマンの画像分割を対象に、光学フィルタとカメラ応答・ニューラルネットワークを同時最適化する画像センシング手法を開発し、RGB方式などと性能比較しているため、植物フェノタイピング手法が中心である。
abstractwe present an end-to-end optimization approach for the simultaneous design of optical filters and green pepper segmentation neural networks.
Automatic plant growth monitoring has received considerable attention in recent years. The demand in this field has created various opportunities, especially for automatic classification using deep learning methods. In this paper, the efficiency of deep learning algorithms in classifying the growth stage of chili plants is studied. Chili is one of the high cash value crops, and automatic identification of chili plant growth stages is essential for crop productivity. Nevertheless, the study on automatic chili plant growth stage classification using deep learning approaches is not widely explored, and this is due to the unavailability of public datasets on the chili plant growth stages. Various deep learning methods, namely Inception V3, ResNet50, and VGG16, were used in the study, and the results have shown that these methods performed well in terms of accuracy and stability when tested on a dataset that consists of 2,320 images of Capsicum annum 'Bird's Eye' plants of various growth stages and imaging conditions. Nevertheless, the results have also shown that the deep learning methods have difficulty classifying images with a complex background where more than one chili plant was captured in an image.
Why it matches plant phenotyping methods唐辛子植物の生育段階という植物状態を画像と深層学習で自動分類し、複数モデルの性能・安定性を比較評価しているため、表現型抽出手法が中心です。
abstractAutomatic plant growth monitoring has received considerable attention in recent years.
Recently, deep-learning techniques have become the foundations for many breakthroughs in the automated identification of plant diseases. In the agricultural sector, many recent visual-computer approaches use deep-learning models. In this approach, a novel predictive analytics methodology to identify Tobacco Mosaic Virus (TMV) and Pepper Huasteco Yellow Vein Virus (PHYVV) visual symptoms on Jalapeño pepper ( Capsicum annuum L.) leaves by using image-processing and deep-learning classification models is presented. The proposed image-processing approach is based on the utilization of Normalized Red-Blue Vegetation Index (NRBVI) and Normalized Green-Blue Vegetation Index (NGBVI) as new RGB-based vegetation indices, and its subsequent Jet pallet colored version NRBVI-Jet NGBVI-Jet as pre-processing algorithms. Furthermore, four standard pre-trained deep-learning architectures, Visual Geometry Group-16 (VGG-16), Xception, Inception v3, and MobileNet v2, were implemented for classification purposes. The objective of this methodology was to find the most accurate combination of vegetation index pre-processing algorithms and pre-trained deep- learning classification models. Transfer learning was applied to fine tune the pre-trained deep- learning models and data augmentation was also applied to prevent the models from overfitting. The performance of the models was evaluated using Top-1 accuracy, precision , recall , and F1-score using test data. The results showed that the best model was an Xception-based model that uses the NGBVI dataset. This model reached an average Top-1 test accuracy of 98.3%. A complete analysis of the different vegetation index representations using models based on deep-learning architectures is presented along with the study of the learning curves of these deep-learning models during the training phase.
Why it matches plant phenotyping methods葉の可視症状をRGB画像処理と深層学習で識別する手法を開発・比較しており、植物病害状態の表現型推定が中心である。
abstracta novel predictive analytics methodology to identify Tobacco Mosaic Virus (TMV) and Pepper Huasteco Yellow Vein Virus (PHYVV) visual symptoms on Jalapeño pepper ( Capsicum annuum L.) leaves by using image-processing and deep-learning classification models is presented.
Reproduction assets foundThe paper publicly releases its authors' analysis code on GitHub and its generated leaf image datasets (RGB plus vegetation-index versions) on Zenodo, both with explicit availability statements.Code · publicThe source code of this article is publicly released and can be downloaded from https://github.com/jrmillan1983/PHYVV_TMV_CNN .Open asset ↗jrmillan1983/PHYVV_TMV_CNNlines:371-472Dataset · publicThe datasets generated during and/or analyzed during the current study are available from https://doi.org/10.5281/zenodo.5500727 (accessed on 19 September 2021).Open asset ↗zenodo · 10.5281/zenodo.5500727lines:474-476Plant phenotyping relevance match · UnverifiedCrossref · checked 15 Sept 2026
Published13 Sept 20212021 IEEE International Conference on Signal and Image Processing Applications (ICSIPA)Cited by 10 · OpenAlex ↗
Measurement and estimation of physical properties of plant leaves have always been considered as important requirements for monitoring and optimizing of plant growth. This study aimed at utilization of image processing and artificial intelligence techniques for non-invasive and non-destructive estimation of bell pepper leaves properties in the first month of growth. Physical properties of bell pepper plant leaves were extracted from RGB images. The algorithm makes use of gradient magnitude and watershed image. Leaf area as the most important index of growth was estimated as a function of other physical parameters including leaf length, width, perimeter etc. Using stereo imaging, the leaf distance from the camera was measured and applied in pixel-wise calculations. Artificial neural networks (ANN) were trained based on a database of actual values of leaf properties (i.e. 311 bell-pepper plant leaves). The success rate of the developed algorithm for detection and separation of leaves was 84.32%. The Multilayer Perceptron (MLP) network could successfully estimate the leaf area values with a validation performance of 0.912.
Why it matches plant phenotyping methodsRGB画像、画像処理、ステレオ計測、ANNを用いて葉面積を推定する手法の開発と検証が中心であり、植物形質の取得・抽出方法に該当する。
abstractThis study aimed at utilization of image processing and artificial intelligence techniques for non-invasive and non-destructive estimation of bell pepper leaves properties
Abstract: Observations today have verified that the average crop yield in India is declining due to illnesses that have affected fully grown plants. Chilli plant production is tough due to the plant's vulnerability to a variety of microorganisms, infectious illnesses, and pests. Infections in the chilli plant impact areas such as the leaves and stems. In the early stages of diagnosing chilli illnesses, leaf characteristics are examined. The leaf image is taken and analyzed to determine the health of the chilli plant. Pesticides are currently being tested on chilli plants on a regular basis without first determining the needs of each plant. This ensures that pesticides are only used when diseased plants are discovered. Keywords: Infections in the chilli plant, chilli illnesses, characteristics are examined, Pesticides are currently being tested on chilli plants.
Why it matches plant phenotyping methods画像処理でチリ植物の葉画像から健康状態・病害を推定することが中心であり、植物病害状態の画像ベース表現型計測に該当する。
abstractThe leaf image is taken and analyzed to determine the health of the chilli plant.
To fulfil the food requirement and economic growth, farming plays a very important role. Thus Farmers are the most important people in the world. Be it the smallest or the largest country, Because of them only we are able to live on the planet. Precision agriculture is the new trending term in the field of technology whose main motive is to reduce the workload of the farmers and increase the productivity of the farms by using technologies. So the aim of this work is to detect the disease of the plant by classifying their leaves using deep learning algorithm. For this work chilli plants are considered, because of their economic importance. And there are various problems in chilli production due to the presence of various micro-organisms and pathogens and The plant disease detection can be done by observing the spot on the leaves of the affected plant. The method here adopting to detect plant diseases is image processing using Dense Net based Convolution neural network (CNN). CNN will be used for leaf image classification and will produce the good results with a good accuracy.
Why it matches plant phenotyping methods病斑を含む植物葉画像から病害を分類する画像解析手法が研究の中心であり、植物の病害状態を直接推定するため、植物フェノタイピング手法に該当します。
abstractthe aim of this work is to detect the disease of the plant by classifying their leaves using deep learning algorithm.
The evolution of food manufacturer in global contribute in national income of the country. Agriculture has been a part of everyone’s life which result in providing food become the building block of every human being. Malaysia is only country experiencing deteriorating development contribute (25.9%) agriculture in Gross Deficient Domestics Product (GDP) while others are fishing (12%), rubber (3.0%) and forestry & logging (6.3%), livestock (15.3%). In line with the development of technology in present century, a lot of methods and technique introduced to upgrowth agriculture sector by focusing to the plant health. The aims of this study are to classify of agriculture plant health through NDVI using image processing. Image processing is a technique representing operations and observation on an image. The images of plant will be captured in this investigation to obtain a photo without (Infra-Red) IR imaging filter. Some of steps must be perform which also include of using multi-function software to gain NDVI values of plant. The main objective in this study is to classify plant health by performing the vegetation index of plant and identify the best machine learning to be applied.
Why it matches plant phenotyping methods植物画像からNDVIを算出し、植物の健康状態を分類する画像処理・機械学習手法が研究の中心であるため。
abstractThe aims of this study are to classify of agriculture plant health through NDVI using image processing.
Agriculture and crop production play an important role in our everyday lives.The primary source of food and clothing is agriculture.Food and clothing are currently being lost due to contaminated crops that decrease production rates.There are a variety of diseases that affect the plant's leaves, fruits, and stem.Bacteria, fungi, viruses, and other microorganisms are the most common causes of plant disease.Diseases are often difficult to monitor, and observations made with the naked eye are unreliable in detecting them.If we can't detect the disease quickly, we won't be able to take the appropriate action.The quality and quantity of goods would improve if pesticides are used less in agriculture.The technique is now mostly used in image processing for the identification of plant diseases.The technology is used in this method for detecting and classifying leaf diseases using SVM classification.Image acquisition, image preprocessing, feature extraction, and classification are all steps in this technology.Banana, pepper, and rice were the three crops we used.A total of 400 leaf sample images were used.From there, 80% will be used for preparation and 20% for research.With an accuracy of 92.99 percent, this device can successfully diagnose and identify the disease.
Why it matches plant phenotyping methods植物葉の画像から病害状態を抽出・分類するSVM手法が研究の中心であり、画像取得から特徴抽出、分類、精度評価まで記述されているため、植物フェノタイピング手法として含める。
abstractThe technology is used in this method for detecting and classifying leaf diseases using SVM classification.
Instinctive detection of infections by carefully inspecting the signs on the plant leaves is an easier and economic way to diagnose different plant leaf diseases. This defines a way in which symptoms of diseased plants are detected utilizing the concept of feature learning (Sulistyo et al., 2020). The physical method of detecting and analyzing diseases takes a lot of time and has chances of making many errors (Sulistyo et al., 2020). So a method has been developed to identify the symptoms by just acquiring the chili plant leaf image. The methodology used involves image database, extracting the region of interest, training and testing images, symptoms/features extraction of the plant image using moments, building of the symptom vector feature dataset, and finding the correlation and similarity between different symptoms of the plant (Sulistyo et al., 2020). This will detect different diseases of the plant.
Why it matches plant phenotyping methods植物葉の画像から病徴を抽出・解析して病害を識別する画像ベースの植物表現型推定手法が研究の中心であるため、収録対象です。
abstracta method has been developed to identify the symptoms by just acquiring the chili plant leaf image
Plant phenotyping relevance match · UnverifiedCrossref · Europe PMC · checked 9 Sept 2026
Effective assessment of pathogen growth can facilitate screening for disease resistance, mapping of resistance loci, testing efficacy of control measures, or elucidation of fundamental host-pathogen interactions. Current methods are often limited by subjective assessments, inability to detect pathogen growth prior to appearance of symptoms, destructive sampling, or limited capacity for replication and quantitative analysis. In this work we sought to develop a real-time, in vivo , high-throughput assay that would allow for quantification of pathogen growth. To establish such a system, we worked with the broad host-range, highly destructive, soil-borne oomycete pathogen, Phytophthora capsici . We used an isolate expressing red fluorescence protein (RFP) to establish a microtiter plate, real-time assay to quantify pathogen growth in live tissue. The system was successfully used to monitor P. capsici growth in planta on cucumber ( Cucumis sativus ) fruit and pepper ( Capsicum annuum ) leaf samples in relation to different levels of host susceptibility. These results demonstrate usefulness of the method in different species and tissue types, allowing for highly replicated, quantitative time-course measurements of pathogen growth in vivo . Analyses of pathogen growth during initial stages of infection preceding symptom development show the importance of very early stages of infection in determining disease outcome, and provide insight into points of inhibition of pathogen growth in different resistance systems.
Why it matches plant phenotyping methods植物組織上の病原体増殖を蛍光でリアルタイム・定量測定する高スループット手法を開発し、複数の植物種・組織で検証している。病原体検出のみでなく、生体植物の感染進行・病害状態に関わる表現型取得が中心である。
abstractIn this work we sought to develop a real-time, in vivo , high-throughput assay that would allow for quantification of pathogen growth.
Potassium is a macro element in plants that is typically supplied to crops in excess throughout the season to avoid a deficit leading to reduced crop yield. Transpiration rate is a momentary physiological attribute that is indicative of soil water content, the plant's water requirements, and abiotic stress factors. In this study, two systems were combined to create a hyperspectral-physiological plant database for classification of potassium treatments (low, medium, and high) and estimation of momentary transpiration rate from hyperspectral images. PlantArray 3.0 was used to control fertigation, log ambient conditions, and calculate transpiration rates. In addition, a semi-automated platform carrying a hyperspectral camera was triggered every hour to capture images of a large array of pepper plants. The combined attributes and spectral information on an hourly basis were used to classify plants into their given potassium treatments (average accuracy = 80%) and to estimate transpiration rate (RMSE = 0.025 g/min, R 2 = 0.75) using the advanced ensemble learning algorithm XGBoost (extreme gradient boosting algorithm). Although potassium has no direct spectral absorption features, the classification results demonstrated the ability to label plants according to potassium treatments based on a remotely measured hyperspectral signal. The ability to estimate transpiration rates for different potassium applications using spectral information can aid in irrigation management and crop yield optimization. These combined results are important for decision-making during the growing season, and particularly at the early stages when potassium levels can still be corrected to prevent yield loss.
Why it matches plant phenotyping methods近接ハイパースペクトル画像とXGBoostを用いて、植物のカリウム処理状態と瞬時蒸散速度を定量・推定するワークフローが研究の中心であり、植物表現型の取得・抽出に該当する。
abstracttwo systems were combined to create a hyperspectral-physiological plant database for classification of potassium treatments (low, medium, and high) and estimation of momentary transpiration rate from hyperspectral images.
Why it matches plant phenotyping methodsチリ葉画像から病害・害虫症状を抽出・識別する画像解析手法を、従来特徴量と深層学習特徴量で比較評価しており、植物の病害状態の表現型取得が中心です。
abstractchili pest and disease features extracted using the traditional approach were compared with features extracted using a deep-learning-based approach
Wet chemistry analysis of agricultural plant materials such as leaves is widely performed to quantify key chemical components to understand plant physiological status. Visible and near-infrared (Vis-NIR) spectroscopy is an interesting tool to replace the wet chemistry analysis, often labour intensive and time-consuming. Hence, this study accesses the potential of Vis-NIR spectroscopy to predict nitrogen (N) and potassium (K) concentration in bell pepper leaves. In the chemometrics perspective, the study aims to identify key Vis-NIR wavelengths that are most correlated to the N and K, and hence, improves the predictive performance for N and K in bell pepper leaves. For wavelengths selection, six different wavelength selection techniques were used. The performances of several wavelength selection techniques were compared to identify the best technique. As a baseline comparison, the partial least-square (PLS) regression analysis was used. The results showed that the Vis-NIR spectroscopy has the potential to predict N and K in pepper leaves with root mean squared error of prediction (RMSEP) of 0.28 and 0.44%, respectively. The wavelength selection in general improved the predictive performance of models for both K and N compared to the PLS regression. With wavelength selection, the RMSEP's were decreased by 19% and 15% for N and K, respectively, compared to the PLS regression. The results from the study can support the development of protocols for non-destructive prediction of key plant chemical components such as K and N without wet chemistry analysis.
Why it matches plant phenotyping methodsVis-NIR分光と波長選択・回帰モデルにより、ベル pepper葉のN・K濃度という植物形質を非破壊推定する方法の開発・比較検証が中心である。
abstractthis study accesses the potential of Vis-NIR spectroscopy to predict nitrogen (N) and potassium (K) concentration in bell pepper leaves.
Reproduction assets foundThe paper's Vis-NIR spectra of 119 dried bell pepper leaves with reference K and N measurements are explicitly stated to be freely available on EcoSIS, making it a public, paper-specific phenotyping dataset. The MATLAB Central link refers only to third-party generic wavelength-selection code, not authors' analysis codeDataset · publicsamples [32]. For spectral measurement, the powder of each leaf was
placed on the probe and covered with a black cover. All spectral data
ranged from 400 to 2400 nm with 5 nm resolution. The data set is freely
available at the official website of ecological spectral information system
(EcoSIS) and can be obtained with the link: https://ecosis.org/package
/fresh-and-dry-pepper-leaf-spectra-with-associated-potassium-and-nit
rogen-measurements.
2.2. Data analysis
The data were partitioned to calibration (60%) and test (40%) set
using the duplex algorithm [33]. The reflectance data were used directly
for the data processing as using chemometric pre-processing methods
may remove theOpen asset ↗EcoSISpdf-raw-page:2 lines:1-78Plant phenotyping relevance match · UnverifiedOpenAlex · checked 13 Sept 2026
Abstract Application of cold storage temperatures below optimum induces a high risk and threat of chilling injury (CI) in the case of sensitive commodities. Sweet pepper belongs to this group of vegetables, so our main objective was to investigate and monitor the effect of non-optimal temperatures (2.5 and 5 °C) induced stress (chilling injury) on kápia type sweet pepper (Capsicum annuum L.) during its postharvest storage by nondestructive quality measuring methods. Fresh, semi-matured (reddish-green colored) samples of ‘Kapitány F1’ cultivar were stored at 2.5, 5 and 10 °C for 7 d followed by 7 d shelf-life. Nondestructive texture measurements were carried out by a purpose built tabletop acoustic stiffness device. Surface color and chlorophyll content related quality indices were evaluated by a chroma meter, a DA-meter® and a chlorophyll fluorescence imaging system. High resolution digital pictures were captured and analyzed for possible CI defects by means of surface color values (normalized RGB, hue and saturation). According to our results, the evaluated quality indices (DA-index®, acoustic stiffness coefficient, surface color parameters; F0, Fm, Fv and Fv/Fm chlorophyll fluorescence parameters) clearly represented the temperature dependent quality changes during low temperature storage, subsequently followed by ambient shelf-life. Samples stored under and at 5 °C showed the chilling temperature stressed symptoms of delayed and partly retarded postharvest ripening, even under simulated shelf-life conditions, but without the onset and manifestation of the characteristic visible symptoms of chilling injury. This may raise doubts and suggest possible future research areas regarding the role of non-optimal cold storage temperatures induced stress, the effect of chilling injury contributing factors and consequences.
Why it matches plant phenotyping methods非破壊センサー、蛍光イメージング、画像解析を用いて収穫後ピーマンの低温ストレスと品質状態を評価する手法が研究の中心であり、単なる生物学的実験の rutin測定ではない。
abstractour main objective was to investigate and monitor the effect of non-optimal temperatures (2.5 and 5 °C) induced stress (chilling injury) on kápia type sweet pepper (Capsicum annuum L.) during its postharvest storage by nondestructive quality measuring methods.
Phytophthora blight of pepper, caused by Phytophthora capsici, is a disease seriously affects pepper production. The aims of this study were to evaluate the control of P. capsici by antagonistic bacteria in pot trials and to score disease severity by using artificial intelligence (AI). For this purpose, six P. capsici isolates used in the study were identified with PCR, and pathogenicity tests were performed. Biological control pot trials were conducted by testing three antagonistic bacteria. At the end of the pot trials, the disease severity in the plants was rated visually. An AI system was also created for the current study. The scores from the AI system were compared with the visual scale scores with different methods. All pathogen isolates were identified as P. capsici according to the PCR results. PcU5 was found to be the most aggressive isolate of the tested P. capsici isolates according to pathogenicity tests, with 45 mm lesion length. Pca17 was found to be an effective bacterial antagonist, with 77% efficiency in pot trials. The AI system, called PhytAi (https://phytai.online), significantly correctly scored the disease severity when compared with the visual scores (r² = 0.96). PhytAi can be used not only by phytopathologists but also by any researcher working in plant sciences who needs visual classification. This is a general classification system that supports scale ratings; the determination of varieties, disease severity measurement, and all kinds of visual classification, identification, etc. can be easily performed without changes in the system thanks to AI.
Why it matches plant phenotyping methods植物病害の視覚的な重症度をAIで推定し、目視スコアと比較検証したシステムが研究の中心であるため、植物フェノタイピング手法として収載する。
abstractto score disease severity by using artificial intelligence (AI)
(1) The objective of the present study was to identify suitable parameters to determine the (degree of) freshness of Bell pepper fruit of three colors (yellow, red, and green) over a two-week period including the occurrence of shrivel using non-destructive real-time measurements (2) Materials and methods: Surface glossiness was measured non-destructively with a luster sensor type CZ-H72 (Keyence Co., Osaka, Japan), a colorimeter, a spectrometer and a profilometer type VR-5200 (Keyence) to obtain RGB images. (3) Results: During storage and shelf life, bell pepper fruit of initially 230-245 g lost 2.9-4.8 g FW per day at 17 °C and 55% rh. Shriveling started at 6-8% weight loss after 4-5 days and became more pronounced. Glossiness decreased from 450-500 a.u. with fresh fruit without shrivel, 280-310 a.u. with moderately shriveled fruit to 80-90 a.u. with severely shriveled fruit irrespective of color against a background of <40 a.u. within the same color, e.g., light red and dark red. Non-invasive color measurements showed no decline in Lab values (chlorophyll content), irrespective of fruit color and degree of shrivel. RGB images, converted into false color images, showed a concomitant increase in surface roughness (Sa) from Sa = ca. 2 µm for fresh and glossy, Sa = ca. 7 µm for moderately shriveled to Sa = ca. 24 µm for severely shriveled rough surfaces of stored pepper fruit, equivalent to a 12-fold increase in surface roughness. The light reflectance peak at 630-633 nm was universal, irrespective of fruit color and freshness. Hence, a freshness index based on (a) luster values ≥ 450 a.u., (b) Sa ≤ 2 µm and (c) the difference in relative reflectance in % between 630 nm and 500 nm is suggested. The latter values declined from ca. 40% for fresh red Bell pepper, ca. 32% after 6 days when shriveling had started, to ca. 21% after 12 days, but varied with fruit color. (4) Conclusion: overall, it can be concluded that color measurements were unsuitable to determine the freshness of Bell pepper fruit, whereas profilometer, luster sensor, and light reflectance spectra were suitable candidates as a novel opto-electronic approach for defining and parametrizing fruit freshness.
Why it matches plant phenotyping methodsピーマン果実の鮮度・萎びという植物器官の状態を、光沢センサー、分光、プロフィロメータ、画像で非破壊的に定量化し、鮮度指標を提案しているため、測定法の開発・応用が中心です。
abstractidentify suitable parameters to determine the (degree of) freshness of Bell pepper fruit
Modern agricultural applications require knowledge about the position and size of fruits on plants. However, occlusions from leaves typically make obtaining this information difficult. We present a novel viewpoint planning approach that builds up an octree of plants with labeled regions of interest (ROIs), i.e., fruits. Our method uses this octree to sample viewpoint candidates that increase the information around the fruit regions and evaluates them using a heuristic utility function that takes into account the expected information gain. Our system automatically switches between ROI targeted sampling and exploration sampling, which considers general frontier voxels, depending on the estimated utility. When the plants have been sufficiently covered with the RGB-D sensor, our system clusters the ROI voxels and estimates the position and size of the detected fruits. We evaluated our approach in simulated scenarios and compared the resulting fruit estimations with the ground truth. The results demonstrate that our combined approach outperforms a sampling method that does not explicitly consider the ROIs to generate viewpoints in terms of the number of discovered ROI cells. Furthermore, we show the real-world applicability by testing our framework on a robotic arm equipped with an RGB-D camera installed on an automated pipe-rail trolley in a capsicum glasshouse.
Why it matches plant phenotyping methods果実の位置・サイズという植物器官形質をRGB-Dセンサで取得・推定する視点計画法を開発し、シミュレーションと実環境で検証しており、フェノタイピング手法が中心である。
abstractWe present a novel viewpoint planning approach that builds up an octree of plants with labeled regions of interest (ROIs), i.e., fruits.
Reproduction assets foundThe paper's viewpoint-planning system source code and the simulated capsicum plant environments used in the experiments are publicly available on GitHub. OctoMap is a generic third-party library, not a paper-specific asset.Code · publicThe source code of our system is available on GitHub 1 1
1
https://github.com/Eruvae/roi_viewpoint_planner .Open asset ↗Eruvae/roi_viewpoint_plannerlines:73-113Plant phenotyping relevance match · UnverifiedarXiv · checked 9 Sept 2026
We present PATHoBot an autonomous crop surveying and intervention robot for glasshouse environments. The aim of this platform is to autonomously gather high quality data and also estimate key phenotypic parameters. To achieve this we retro-fit an off-the-shelf pipe-rail trolley with an array of multi-modal cameras, navigation sensors and a robotic arm for close surveying tasks and intervention. In this paper we describe PATHoBot design choices made to ensure proper operation in a commercial glasshouse environment. As a surveying platform we collect a number of datasets which include both sweet pepper and tomatoes. We show how PATHoBot enables novel surveillance approaches by first improving our previous work on fruit counting by incorporating wheel odometry and depth information. We find that by introducing re-projection and depth information we are able to achieve an absolute improvement of 20 points over the baseline technique in an "in the wild" situation. Finally, we present a 3D mapping case study, further showcasing PATHoBot's crop surveying capabilities.
Why it matches plant phenotyping methods温室作物の表現型パラメータ推定を目的とするロボット基盤を設計し、果実カウント手法を深度情報等で改善・評価しているため、表現型取得技術が中心である。
abstractThe aim of this platform is to autonomously gather high quality data and also estimate key phenotypic parameters.
Pepper ( Capsicum spp.) fruit-related traits are critical determinants of quality. These traits are controlled by quantitatively inherited genes for which marker-assisted selection (MAS) has proven insufficiently effective. Here, we evaluated the potential of genomic selection, in which genotype and phenotype data for a training population are used to predict phenotypes of a test population with only genotype data, for predicting fruit-related traits in pepper. We measured five fruit traits (fruit length, fruit shape, fruit width, fruit weight, and pericarp thickness) in 351 accessions from the pepper core collection, including 229 Capsicum annuum , 48 Capsicum baccatum , 48 Capsicum chinense , 25 Capsicum frutescens , and 1 Capsicum chacoense in 4 years at two different locations and genotyped these accessions using genotyping-by-sequencing. Among the whole core collection, considering its genetic distance and sexual incompatibility, we only included 302 C. annum complex (229 C. annuum , 48 C. chinense , and 25 C. frutescens ) into further analysis. We used phenotypic and genotypic data to investigate genomic prediction models, marker density, and effects of population structure. Among 10 genomic prediction methods tested, Reproducing Kernel Hilbert Space (RKHS) produced the highest prediction accuracies (measured as correlation between predicted values and observed values) across the traits, with accuracies of 0.75, 0.73, 0.84, 0.83, and 0.82 for fruit length, fruit shape, fruit width, fruit weight, and pericarp thickness, respectively. Overall, prediction accuracies were positively correlated with the number of markers for fruit traits. We tested our genomic selection models in a separate population of recombinant inbred lines derived from two parental lines from the core collection. Despite the large difference in genetic diversity between the training population and the test population, we obtained moderate prediction accuracies of 0.32, 0.34, 0.50, and 0.48 for fruit length, fruit shape, fruit width, and fruit weight, respectively. This use of genomic selection for fruit-related traits demonstrates the potential use of core collections and genomic selection as tools for crop improvement.
Why it matches plant phenotyping methods遺伝型から果実形質を予測する複数のゲノム予測モデルを比較し、別集団で予測精度を検証しており、植物形質推定法が研究の中心です。
abstractHere, we evaluated the potential of genomic selection, in which genotype and phenotype data for a training population are used to predict phenotypes of a test population with only genotype data, for predicting fruit-related traits in pepper.
Deep Learning (DL) is a high capable machine learning algorithm which composed the advanced image processing as feature learning and supervised learning with detailed models with many hidden layers and neurons. DL demonstrated its efficiency and robustness in many big data problems, computer vision, and more. Whereas it has an increasing popularity day by day, it has still some deficiencies to construe the relationship between learned feature maps and spatial information. Capsule network (CapsNET) is proposed to overcome the shortcoming by excluding the pooling layer from the architecture and transferring spatial information between layers by capsule. In this paper, CapsNET architecture was proposed to evaluate the performance of the model on classification of plant leaf diseases using simple reduced capsules on leaf images. Plant leaf diseases are common and prevalent diseases that disrupt harvesting and yielding for agriculture. CapsNET has capability of detailed analysis for even small stains that may lead seed dressing time and duration. The proposed CapsNET model aimed at assessing the applicability of various feature learning models and enhancing the learning capacity of the DL models for bell pepper plants. The healthy and diseased leaf images were fed into the CapsNET. The proposed CapsNET model reached high classification performance rates of 95.76%, 96.37%, and 97.49% for accuracy, sensitivity, and specificity, respectively.
Why it matches plant phenotyping methods植物葉画像から病害状態を分類するCapsNETモデルを提案・評価しており、病徴の画像ベース推定が研究の中心である。
abstractIn this paper, CapsNET architecture was proposed to evaluate the performance of the model on classification of plant leaf diseases using simple reduced capsules on leaf images.
Monitoring the growth of fruit vegetables is essential for the automation of cultivation management, and harvest. The objective of this study is to demonstrate that the current sensor technology can monitor the growth and yield of fruit vegetables such as tomato, cucumber, and paprika. We estimated leaf area, leaf area index (LAI), and plant height using coordinates of polygon vertices from plant and canopy surface models constructed using a three-dimensional (3D) scanner. A significant correlation was observed between the measured and estimated leaf area, LAI, and plant height (R 2 > 0.8, except for tomato LAI). The canopy structure of each fruit vegetable was predicted by integrating the estimated leaf area at each height of the canopy surface models. A linear relationship was observed between the measured total leaf area and the total dry weight of each fruit vegetable; thus, the dry weight of the plant can be predicted using the estimated leaf area. The fruit weights of tomato and paprika were estimated using the fruit solid model constructed by the fruit point cloud data extracted using the RGB value. A significant correlation was observed between the measured and estimated fruit weights (tomato: R 2 = 0.739, paprika: R 2 = 0.888). Therefore, it was possible to estimate the growth parameters (leaf area, plant height, canopy structure, and yield) of different fruit vegetables non-destructively using a 3D scanner.
Why it matches plant phenotyping methods3Dスキャナーと点群・3Dモデル解析を用いて、葉面積、LAI、草丈、樹冠構造、果実重量を非破壊推定し、実測値との相関で技術検証しているため、植物表現型取得法が中心である。
abstractWe estimated leaf area, leaf area index (LAI), and plant height using coordinates of polygon vertices from plant and canopy surface models constructed using a three-dimensional (3D) scanner.
This paper presents a proposed system that is used to classify and detect plant leaf diseases using image processing and deep learning techniques. The proposed system consists of two methods for classification and makes a comparison between them. The first method is based on the support vector machine (SVM) algorithm and consisted of several stages leading to the classification stage. In our work, specific types of plants are selected, which are tomatoes, pepper, and potatoes, as they are the most common types of plants in the world in general and in Iraq in particular. The second method used the convolution Neural network (CNN) for classification. In these two methods, 15 classes were classified, including 12 classes for diseases of different plants that were detected, such as bacteria, fungi, etc., and three classes for healthy leaves. The result of the comparison shows the preference of the CNN algorithm over the SVM algorithm in terms of accuracy and time, which makes an effective and accurate system in the detection and classification of plant leaf diseases.
Why it matches plant phenotyping methods植物葉の画像から病害状態を検出・分類する画像処理および深層学習手法を提案し、SVMとCNNを比較評価しているため、植物フェノタイピング手法が中心です。
abstractThis paper presents a proposed system that is used to classify and detect plant leaf diseases using image processing and deep learning techniques.
Plant phenotyping relevance match · UnverifiedCrossref · Europe PMC · checked 14 Sept 2026
Summary Efficient seed germination and establishment are important traits for field and glasshouse crops. Large‐scale germination experiments are laborious and prone to observer errors, leading to the necessity for automated methods. We experimented with five crop species, including tomato, pepper, Brassica, barley, and maize, and concluded an approach for large‐scale germination scoring. Here, we present the SeedGerm system, which combines cost‐effective hardware and open‐source software for seed germination experiments, automated seed imaging, and machine‐learning based phenotypic analysis. The software can process multiple image series simultaneously and produce reliable analysis of germination‐ and establishment‐related traits, in both comma‐separated values (CSV) and processed images (PNG) formats. In this article, we describe the hardware and software design in detail. We also demonstrate that SeedGerm could match specialists’ scoring of radicle emergence. Germination curves were produced based on seed‐level germination timing and rates rather than a fitted curve. In particular, by scoring germination across a diverse panel of Brassica napus varieties, SeedGerm implicates a gene important in abscisic acid (ABA) signalling in seeds. We compared SeedGerm with existing methods and concluded that it could have wide utilities in large‐scale seed phenotyping and testing, for both research and routine seed technology applications.
Why it matches plant phenotyping methods種子発芽・定着形質の画像取得と機械学習による自動抽出を行うハードウェア/ソフトウェア基盤を詳細に開発・検証しており、フェノタイピング手法が研究の中心である。
abstractIn this article, we describe the hardware and software design in detail.
The effect of camera viewpoint and fruit orientation on the performance of a sweet pepper maturity level classification algorithm was evaluated. Image datasets of sweet peppers harvested from a commercial greenhouse were collected using two different methods, resulting in 789 RGB-Red Green Blue (images acquired in a photocell) and 417 RGB-D-Red Green Blue-Depth (images acquired by a robotic arm in the laboratory), which are published as part of this paper. Maturity level classification was performed using a random forest algorithm. Classifications of maturity level from different camera viewpoints, using a combination of viewpoints, and different fruit orientations on the plant were evaluated and compared to manual classification. Results revealed that: (1) the bottom viewpoint is the best single viewpoint for maturity level classification accuracy; (2) information from two viewpoints increases the classification by 25 and 15 percent compared to a single viewpoint for red and yellow peppers, respectively, and (3) classification performance is highly dependent on the fruit's orientation on the plant.
Why it matches plant phenotyping methodsスイートペッパー果実の成熟度という植物形質を、画像取得視点・果実向き・分類アルゴリズムの性能評価により推定しており、フェノタイピング手法の評価が中心である。
abstractThe effect of camera viewpoint and fruit orientation on the performance of a sweet pepper maturity level classification algorithm was evaluated.
Some leaf area (LA) estimation models have been developed for different plants under optimum conditions, but to date, none has been developed to model for those grown under stress conditions. In this study, LA of bell pepper grown under different levels of irrigation water salinity (IWS) and irrigation regimes (IR) were estimated by means of comparing different procedures including a simple model derived from ellipse area (EM), parabolic model (PM), geometric model (GM), multiple linear regression analysis (MLR), and artificial neural networks (ANN). To this end, two experiments were carried out under greenhouse conditions. First, the LA of bell peppers grown under five IWS levels were identified. In the second experiment, LA was determined under four different IR. Besides the general models elicited from EM, PM, GM, MLR, and ANN for each stress condition, prediction models of the bell peppers for each treatment under both stress conditions also were validated. Performance of the models also were evaluated using root mean square errors (RMSE), mean absolute errors (MAE), coefficient of determination (R²) and a Taylor diagram, which illustrates the accuracy of the models in a concise statistical analysis of how well the correlation (r) and standard deviation (SD) patterns match. Based on these results, the ANN model produced more reliable LA estimations compared to MLR, EM, PM, and GM. The R², RMSE and MAE values were ranged 0.96–0.99, 1.05–2.99 cm², and 0.78–1.12 cm² in all ANN models. Overall, the ANN models are a valuable tool to investigate and understand the estimation of the LA of the bell peppers grown under different levels of IWS and IR.
Why it matches plant phenotyping methodsストレス条件下のピーマンの葉面積という植物形質を、複数の幾何・統計・ANNモデルで推定し、精度検証・比較しており、表現型取得手法が研究の中心である。
titleLeaf area modeling of bell pepper (Capsicum annuum L.) grown under different stress conditions by soft computing approaches
One of the essential factors in the root zone environment that affects plant growth is temperature. Determining the optimal root zone temperature condition in a hydroponic system during cultivation could lead to an improvement in plant growth. An optimal control strategy can be determined by identifying the eco-physiological process using a dynamic model. However, it is difficult to develop a dynamic model of the responses of plant growth to root zone temperature because the eco-physiological processes of plants are quite complicated. We propose an intelligent approach that can deal with this complex system. Non-linear autoregressive with exogenous input (NARX) neural networks were used to develop a dynamic model of the responses of plant growth to root zone temperature. The responses of chili pepper plant growth as affected by root zone temperature were measured during 60 days of cultivation inside a growth chamber using a non-destructive and continuous system based on a load cell. Five datasets of dynamic responses of plant growth were obtained for system identification. The results suggest that the application of a neural network is useful for modeling the dynamic response of plant growth to root zone temperature in hydroponic cultivation, with promising performance.
Why it matches plant phenotyping methods植物成長を連続・非破壊に測定するロードセル系と、成長応答を推定するNARXニューラルネットワーク動的モデルが研究の中心であり、植物成長という形質の取得・モデル化手法に該当する。
abstractWe propose an intelligent approach that can deal with this complex system. Non-linear autoregressive with exogenous input (NARX) neural networks were used to develop a dynamic model of the responses of plant growth to root zone temperature.
Reproduction assets foundThe paper cites the authors' own public Matlab program script for the NARX modeling of plant growth response to root zone temperature, hosted on GitHub (reference 47), which qualifies as a paper-specific public analysis code asset. No public phenotype dataset deposit is stated; the five measurement datasets are not明确lyCode · publicAji, G. K.; Hatou, K.; Morimoto, T. Matlab Program Script for Modeling the Dynamic Response of Plant
Growth to Root Zone Temperature in Hydroponic Chili Pepper Plant using Neural Network Available
online: https://github.com/mradjie/narx‐plant‐growthOpen asset ↗mradjie/narx‐plant‐growthpdf-page:14 lines:1-47Plant phenotyping relevance match · UnverifiedEurope PMC · checked 9 Sept 2026
Background Many metals are essential for plants and humans. Knowledge of metal distribution in plant tissues in vivo contributes to the understanding of physiological mechanisms of metal uptake, accumulation and sequestration. For those studies, X-rays are a non-destructive tool, especially suited to study metals in plants. Results We present microfluorescence imaging of trace elements in living plants using a customized benchtop X-ray fluorescence machine. The system was optimized by additional detector shielding to minimize stray counts, and by a custom-made measuring chamber to ensure sample integrity. Protocols of data recording and analysis were optimised to minimise artefacts. We show that Zn distribution maps of whole leaves in high resolution are easily attainable in the hyperaccumulator Noccaea caerulescens . The sensitivity of the method was further shown by analysis of micro- (Cu, Ni, Fe, Zn) and macronutrients (Ca, K) in non-hyperaccumulating crop plants (soybean roots and pepper leaves), which could be obtained in high resolution for scan areas of several millimetres. This allows to study trace metal distribution in shoots and roots with a wide overview of the object, and thus avoids making conclusions based on singular features of tiny spots. The custom-made measuring chamber with continuous humidity and air supply coupled to devices for imaging chlorophyll fluorescence kinetic measurements enabled direct correlation of element distribution with photosynthesis. Leaf samples remained vital even after 20 h of X-ray measurements. Subtle changes in some of photosynthetic parameters in response to the X-ray radiation are discussed. Conclusions We show that using an optimized benchtop machine, with protocols for measurement and quantification tailored for plant analyses, trace metal distribution can be investigated in a reliable manner in intact, living plant leaves and roots. Zinc distribution maps showed higher accumulation in the tips and the veins of young leaves compared to the mesophyll tissue, while in the older leaves the distribution was more homogeneous.
Why it matches plant phenotyping methods生きた植物の元素分布を定量・可視化するX線蛍光イメージング装置と測定・解析プロトコルを最適化し、植物試料で性能を実証しており、フェノタイピング手法が中心である。
abstractWe present microfluorescence imaging of trace elements in living plants using a customized benchtop X-ray fluorescence machine.
A novel hyperspectral-physiological system that monitors plants dynamic response to abiotic alterations was developed. The system is a sensor-to-plant platform which can determine the optimal time of day during which physiological traits can be successfully identified via spectral means. The directly measured traits include momentary and daily transpiration rates throughout the daytime and daily and periodical plant weight loss and gain. The system monitored and evaluated pepper plants response to varying levels of potassium fertilization. Significant momentary transpiration rates differences were found between the treatments during 07:00–10:00 and 14:00–17:00. The simultaneous frequently measured high-resolution spectral data provided the means to correlate the two measured data sets. Significant correlation coefficients between the spectra and momentary transpiration rates resulted with a selection of three bands (ρ523, ρ697 and ρ818nm) that were used to capture transpiration rate differences using a normalized difference formula during the morning, noon and the afternoon. These differences also indicated that the best results are not always obtained when spectral (remote or proximal) measurements are typically preformed around noon (when solar illumination is the highest). Valuable information can be obtained when the spectral measurements are timed according to the plants’ dynamic physiological status throughout the day, which may vary among plant species and should be considered when planning remote sensing data acquisition.
Why it matches plant phenotyping methods植物の蒸散率・体重変化をスペクトル情報と組み合わせて取得・推定するセンサー連携フェノタイピングシステムの開発が中心であり、施肥試験はその評価に用いられている。
abstractA novel hyperspectral-physiological system that monitors plants dynamic response to abiotic alterations was developed.
Abstract has not been obtained from indexed metadata or an accessible article page.
Why it matches plant phenotyping methodsタイトルから、トウガラシ遺伝資源のフェノミクスと高スループットデジタルツールによる品種類型化が研究の中心と判断でき、植物形質計測手法の適用・プラットフォーム研究に該当する。抄録がないため詳細な技術内容には不確実性が残る。
titlePhenomics of elite heirlooms of peppers (Capsicum annuum L.) from the Spanish centre of diversity: Conventional and high-throughput digital tools towards varietal typification
Abstract Phenotyping for vegetable fruit quality traits can involve laborious postharvest and biochemical assays, decreasing efficiency of data collection. Portable devices that are easy to use and withstand in‐field conditions to non‐destructively and accurately quantify internal fruit quality traits would greatly enhance efficiency in breeding programs. We evaluated a hand‐held quality spectrophotometer, the Felix‐750, as an in‐field tomato ( Solanum lycopersicum L.) and pepper ( Capsicum annuum ) high‐throughput phenotyping tool. Fruit quality traits included pH, soluble solids, carotenoids, and shrink in germplasm grown in replicated split‐plot field trials. Germplasm included elite inbred cultivars and introgression lines of tomato, and diverse hybrid and open‐pollinated cultivars of pepper. Our study employed a multi‐faceted approach to evaluate the use of the Felix‐750 in a plant breeding program. Our approach included chemometrics and trait‐based partial least squares regression modeling, and examination of patterns in the λ ‐specific spectroscopy data based on variables relevant to genetic, fruit, and environmental factors using principal component analysis and biplots. Results of our study revealed: (a) the scope and limitations of the Felix‐750 in fruit quality trait assessment based on the range of predictive power of partial least squares models; (b) insights into the complex relationships of spectroscopy data with genetic diversity, fruit biology and biochemistry, and factors related to environment. Additional research on the Felix‐750 is needed to determine its potential applications at early and later stages of a breeding pipeline. We also suggest researchers explore more advanced chemometric tools and 3‐D fruit hyperspectral imaging approaches.
Why it matches plant phenotyping methods携帯型分光計を用いた果実品質形質の非破壊・高スループット計測について、予測性能と適用限界を評価しており、フェノタイピング手法が中心である。
titleEvaluation of a hand‐held spectrophotometer as an in‐field phenotyping tool for tomato and pepper fruit quality
The improvement of existing analyses that access the physiological quality of seeds and the inclusion of nondestructive techniques represent significant progress to the seed sector. Despite being prominent, the use of x‐ray is hindered by the fact that radiographic images are, in general, analyzed subjectively. Therefore, this study aimed at investigating the relationship between the seed internal morphology, accessed via x‐ray images, and the physiological quality of habanero pepper seeds. The seeds were harvested from fruits at three maturity stages and then kept in post‐harvest storage for different periods. Initially, radiographs were generated and subjected to automated image analysis, using the ImageJ software. The parameters area, perimeter, circularity, relative density, integrated density, and percentage of seed filling were evaluated. After the x‐ray testing, the seeds were tested for germination and vigor. It was observed that postharvest storage increased the relative density of seed tissues, as well as seed filling and germination for all stages of maturity. Positive and significant correlations were found between tissue density parameters, evaluated by image analysis, with seed germination, germination speed and seed viability, while negative correlations were observed with seed dormancy. In general, the automated radiograph analysis of habanero pepper seeds is a promising method to obtain physical variables of seeds, such as relative density, integrated density, and seed filling. Habanero pepper seeds obtained from yellow and orange fruits exhibit higher physiological quality. The storage of these fruits after harvested before seed extraction is a good alternative to improve the seed physiological quality.
Why it matches plant phenotyping methodsX線画像をImageJで自動解析し、種子の内部形態・密度・充填率などの物理形質を抽出する手法が研究の中心であり、発芽・活力との関連も検証している。
abstractradiographic images are, in general, analyzed subjectively. Therefore, this study aimed at investigating the relationship between the seed internal morphology, accessed via x‐ray images, and the physiological quality of habanero pepper seeds.
Fruits are the dominant sinks for assimilates. At optimal conditions, assimilates supply can meet the demand of fruits and those of the vegetative organs; however, extreme circumstances such as strong sink strength or an environmental stress may disturb this fine balance. While most studies focus on aboveground parameters, information regarding root growth dynamics under variable sink strength are scarce. The objective of this study was to evaluate the effect of sink strength (represented by fruit load) and salinity on bell-pepper root development. Three levels of fruit load were combined with two salinity levels in plants grown in an aeroponic system. Root growth was determined both by root capacitance and destructive measurements. Salinity and sink strength significantly affected root, shoot and fruit growth dynamics. Root growth was less affected by fruit load. Salinity stress was negatively associated with shoot growth, but after an acclimation period, salinity enhanced root development. Additionally, this study shows for the first time that root capacitance is a valid approach for non-destructive measurement of root development in aeroponic systems. The good correlation measured by us (r 2 0.86) opens new opportunities for continuous root growth monitoring in aeroponic systems in the future.
Why it matches plant phenotyping methods根の発達を評価する生物学的実験が主目的だが、根容量法の非破壊的な根成長測定法としての妥当性を明示的に検証しており、方法論的貢献が中心的に含まれるため。
abstractRoot growth was determined both by root capacitance and destructive measurements.
Vegetation health assessment by using airborne multispectral images throughout crop production cycles, among other precision agriculture technologies, is an important tool for modern agriculture practices. However, to really take advantage of crop fields imagery, specialized analysis techniques are needed. In this paper we present a geographic object-based image analysis (GEOBIA) approach to examine a set of very high resolution (VHR) multispectral images obtained by the use of small unmanned aerial vehicles (UAVs), to evaluate plant health states and to generate cropland maps for Capsicum annuum L. The scheme described here integrates machine learning methods with semi-automated training and validation, which allowed us to develop an algorithmic sequence for the evaluation of plant health conditions at individual sowing point clusters over an entire parcel. The features selected at the classification stages are based on phenotypic traits of plants with different health levels. Determination of areas without data dependencies for the algorithms employed allowed us to execute some of the calculations as parallel processes. Comparison with the standard normalized difference vegetation index (NDVI) and biological analyses were also performed. The classification obtained showed a precision level of about 95 % in discerning between vegetation and non-vegetation objects, and clustering efficiency ranging from 79 % to 89 % for the evaluation of different vegetation health categories, which makes our approach suitable for being incorporated at C. annuum crop's production systems, as well as to other similar crops. This methodology can be reproduced and adjusted as an on-the-go solution to get a georeferenced plant health estimation.
Why it matches plant phenotyping methodsUAVマルチスペクトル画像とGEOBIA・機械学習を用いて、個体群クラスター単位の植物健康状態を推定する手法が研究の中心であり、検証と既存指標との比較も行っている。
abstractIn this paper we present a geographic object-based image analysis (GEOBIA) approach to examine a set of very high resolution (VHR) multispectral images obtained by the use of small unmanned aerial vehicles (UAVs), to evaluate plant health states
For years, the main emphasis of industrial pepper production has been the creation of large-fruited high-yielding varieties. This has led to the exploitation of pepper (Capsicum annuum L.) varieties with a significantly narrow genetic background. The Balkan region has a range of valuable local landraces that are noticeably decreasing in prevalence and may be lost. In this study, we utilized Tomato Analyzer (TA) to characterize the intra- and inter-varietal type fruit diversity using 50 different fruit shape, size, and color descriptors of a Balkan pepper collection. The collection comprised of 168 diverse accessions and collected from 62 locations and 31 districts in six different Balkan countries. Fruit shape was the main trait used for visual accession grouping into 5 different varietal groupings of elongated, round, conical, bell, and pumpkin shape, respectively. Beyond visual grouping, hierarchical cluster analysis grouped the evaluated accessions into eight distinct clusters based on fruit shape, size, surface, and color. In total, 47 TA descriptors were identified to contribute to the total variation, with first two components explaining 54% of variation, and 90% of variation contained in 12 components. Among TA descriptors, fruit size and proximal/distal fruit end shape contributed to component 1 variation whereas fruit perimeter, area and color contributed to component 2. We hope that this research will assist pepper breeding and genetic resources communities to better understand Balkan pepper fruit diversity and develop pepper varieties with desirable fruit traits. Quantification of fruit diversity could be crucial for further investigation into the genetic determinants of fruit shape and size by a genome-wide association study.
Why it matches plant phenotyping methodsTomato Analyzerを用いた果実形状・サイズ・色の大規模な定量化が研究の中心であり、植物表現型取得ツールの実質的な適用に該当する。
abstractIn this study, we utilized Tomato Analyzer (TA) to characterize the intra- and inter-varietal type fruit diversity using 50 different fruit shape, size, and color descriptors of a Balkan pepper collection.
Non-destructive plant growth parameters measurement is an important concern in automatic-seedling transplanting. Recently, several image-based monitoring approaches have been proposed and potentially developed for several agricultural applications. The presented study proposed and developed a RealSense-based machine vision system for the close-shot seedling-lump integrated monitoring. The strategy was based on the close-shot depth information. Further, the point cloud clustering and suitable algorithms were applied to obtain the segmentation of 3D seedling models. In addition, the data processing pipeline was developed to assess the different morphological parameter of 4 different seedling varieties. The experiments were carried out with 4 different seedling varieties (pepper, tomato, cucumber, and lettuce) and trained under different light conditions (light and dark). Moreover, analysis results showed that there was not significantly different (p < 0.05) found towards light and dark environments due to close-shot near-infrared detection. However, the results revealed that the stem diameter relationship between RealSense and the manual method was found for R2 = 0.68 cucumber, R2 = 0.54 tomato, R2 = 0.35 pepper, and R2 = 0.58 lettuce seedlings. Whereas, the seedling height relationship between RealSense and the manual method was found higher than R2 = 0.99, 0.99, 0.99, and 0.99 for pepper, tomato, cucumber, and lettuce, respectively. Based on the experiment results, it was concluded that the RGB-D integrated monitoring system with the purposed method could be practiced for nursery seedlings most promisingly without high labour requirements in terms of ease of use. The system revealed a good sturdiness and relevance for plant growth monitoring. Additionally, it has the perspective for future practical value to real-time vision servo operations for transplanting robots.
Why it matches plant phenotyping methodsRealSense深度カメラ、点群クラスタリング、処理パイプラインを開発し、苗の形態形質を非破壊測定して手動法と検証しているため、植物フェノタイピング手法が中心である。
abstractThe presented study proposed and developed a RealSense-based machine vision system for the close-shot seedling-lump integrated monitoring.
Plant phenotyping relevance match · UnverifiedOpenAlex · Europe PMC · checked 9 Sept 2026
The improvement of crop productivity under abiotic stress is one of the biggest challenges faced by the agricultural scientific community. Despite extensive research, the research-to-commercial transfer rate of abiotic stress-resistant crops remains very low. This is mainly due to the complexity of genotype × environment interactions and in particular, the ability to quantify the dynamic plant physiological response profile to a dynamic environment. Most existing phenotyping facilities collect information using robotics and automated image acquisition and analysis. However, their ability to directly measure the physiological properties of the whole plant is limited. We demonstrate a high-throughput functional phenotyping system (HFPS) that enables comparing plants’ dynamic responses to different ambient conditions in dynamic environments due to its direct and simultaneous measurement of yield-related physiological traits of plants under several treatments. The system is designed as one-to-one (1:1) plant–[sensors+controller] units, i.e., each individual plant has its own personalized sensor, controller and irrigation valves that enable (i) monitoring water-relation kinetics of each plant–environment response throughout the plant's life cycle with high spatiotemporal resolution, (ii) a truly randomized experimental design due to multiple independent treatment scenarios for every plant, and (iii) reduction of artificial ambient perturbations due to the immobility of the plants or other objects. In addition, we propose two new resilience-quantifying-related traits that can also be phenotyped using the HFPS: transpiration recovery rate and night water reabsorption. We use the HFPS to screen the effects of two commercial biostimulants (a seaweed extract—ICL-SW, and a metabolite formula—ICL-NewFo1) on Capsicum annuum under different irrigation regimes. Biostimulants are considered an alternative approach to improving crop productivity. However, their complex mode of action necessitates cost-effective pre-field phenotyping. The combination of two types of treatment (biostimulants and drought) enabled us to evaluate the precision and resolution of the system in investigating the effect of biostimulants on drought tolerance. We analyze and discuss plant behavior at different stages, and assess the penalty and trade-off between productivity and resilience. In this test case, we suggest a protocol for the screening of biostimulants’ physiological mechanisms of action.
Why it matches plant phenotyping methods植物の動的な生理形質を直接・高スループットに測定するシステムを開発し、新規形質の定義と性能評価を行ったうえで、乾燥・バイオスティミュラント処理に適用しているため、フェノタイピング手法が中心的です。
abstractWe demonstrate a high-throughput functional phenotyping system (HFPS) that enables comparing plants’ dynamic responses to different ambient conditions in dynamic environments due to its direct and simultaneous measurement of yield-related physiological traits of plants under several treatments.
For many robotic harvesting applications, position and angle between plant parts is required to optimally position the end-effector before attempting to approach, grasp and cut the product. A method for estimating the angle between plant parts, e.g. stem and fruit, is presented to support the optimisation of grasp pose for harvest robots. The hypothesis is that from colour images, this angle in the horizontal plane can be accurately derived under unmodified greenhouse conditions. It was hypothesised that the location of a fruit and stem could be inferred in the image plane from sparse semantic segmentations. The paper focussed on 4 sub-tasks for a sweet-pepper harvesting robot. Each task was evaluated under 3 conditions: laboratory, simplified greenhouse and unmodified greenhouse. The requirements for each task were based on the end-effector design that required a 25° positioning accuracy. In Task I, colour image segmentation for classes back-ground, fruit and stem plus wire was performed, meeting the requirement of an intersection-over-union > 0.58. In Task II, the stem pose was estimated from the segmentations. In Task III, centres of the fruit and stem were estimated from the output of previous tasks. Both centre estimations In Tasks II and III met the requirement of 25 pixel accuracy on average. In Task IV, the centres were used to estimate the angle between the fruit and stem, meeting the accuracy requirement of 25° for 73% of the cases. The work impacted on the harvest performance by increasing its success rate from 14% theoretically to 52% in practice under unmodified conditions.
Why it matches plant phenotyping methods色画像から果実と茎の位置・角度という植物器官の幾何学的形質を推定する手法を開発し、複数条件で精度検証している。単なる収穫対象の検出ではなく、器官間角度を定量化しているため対象範囲に該当する。
abstractA method for estimating the angle between plant parts, e.g. stem and fruit, is presented
A current bottleneck of state-of-the-art machine learning methods for image segmentation in agriculture, e.g. convolutional neural networks (CNNs), is the requirement of large manually annotated datasets on a per-pixel level. In this paper, we investigated how related synthetic images can be used to bootstrap CNNs for successful learning as compared to other learning strategies. We hypothesise that a small manually annotated empirical dataset is sufficient for fine-tuning a synthetically bootstrapped CNN. Furthermore we investigated (i) multiple deep learning architectures, (ii) the correlation between synthetic and empirical dataset size on part segmentation performance, (iii) the effect of post-processing using conditional random fields (CRF) and (iv) the generalisation performance on other related datasets. For this we have performed 7 experiments using the Capsicum annuum (bell or sweet pepper) dataset containing 50 empirical and 10,500 synthetic images with 7 pixel-level annotated part classes. Results confirmed our hypothesis that only 30 empirical images were required to obtain the highest performance on all 7 classes (mean IOU = 0.40) when a CNN was bootstrapped on related synthetic data. Furthermore we found optimal empirical performance when a VGG-16 network was modified to include à trous spatial pyramid pooling. Adding CRF only improved performance on the synthetic data. Training binary classifiers did not improve results. We have found a positive correlation between dataset size and performance. For the synthetic dataset, learning stabilises around 3000 images. Generalisation to other related datasets proved possible.
Why it matches plant phenotyping methods植物画像から部位を画素単位で抽出するCNNセグメンテーション手法の開発・比較・汎化評価が研究の中心であり、植物フェノタイピング手法に該当する。
titleSynthetic bootstrapping of convolutional neural networks for semantic plant part segmentation
Pepper / chilliRoot2D/3D reconstructionRoot system architecture
Background Root is the principal part of plants to absorb water and nutrient, anchor the plant and affect yield and quality. Non-destructive detection of root traits is difficult to perform due to the hidden nature of the root. Therefore, improved methods to measure roots are necessary to support plant breeding, and optimization of cultivation and management. In this study, we present an adaptive minirhizotron along with installation patterns to focus on micro and local changes in multipoint of pepper roots. Results The method is to improve minirhizotron by reducing its size to a microrhizotron (1.1 × 1.1 × 1.2 cm) and improving installation validity and rationality according to spatial distribution characteristics of Capsicum annuum root system. This adaptive minirhizotron could acquire root image in multipoint, and through image processing, root traits such as root length (including very fine roots or root hairs) and root width could be calculated. In order to install the microrhizotron reasonably and effectively, root system architecture ( Capsicum annuum ) was reconstructed using a three-dimensional caliper, and were quantified in circumferential distribution, vertical direction and root extension. The results showed that most lateral roots were constrained to 45° in horizontal direction to root initial position; Vertical angles were large, almost perpendicular to the root center line at initial position, and it became smaller when roots began to deepen. Root length density decreased with the increase of distance to plant center. According to Capsicum annuum root system traits, totally 8 installation methods were tested and verified to determine high probability of root interception. Horizontal angle 45° showed much higher interception probability than that of 90°. Vertical angle 45° has slightly higher root interception probability than that of 30°. Installation pattern horizontal angle 45° + radius 30 mm + vertical angle 45° showed the best performance in root interception with probability of 96.7%, followed by pattern horizontal angle 45° + radius 30 mm + vertical angle 30°. Comparison experiment showed that when root hair and very fine root were excluded, relative error was 12.1% between microrhizotron and soil sampling in root length, and 15.4% in root diameter. Microrhizotron was able to observe fine roots about 0.1 mm in diameter. Conclusion A new adaptive minirhizotron has been established for nondestructive observation on local and micro changes of roots in multipoint, and its application and installation patterns has been suggested according to root architecture traits. The microrhizotron can be used to study a wide range of research questions focused on quantitative trait locus analysis, root width changes, and root hair growth.
Why it matches plant phenotyping methodsコショウ根系の非破壊画像取得法を開発し、設置条件と根長・根径の測定精度を検証しており、植物表現型取得が研究の中心である。
abstractwe present an adaptive minirhizotron along with installation patterns to focus on micro and local changes in multipoint of pepper roots.
Optical sensors are a promising approach for assessing nitrogen (N) status of vegetable crops. However, their potential may be undermined if time of day influences measurements. This study evaluated the effects of time of day and N addition on measurements, made with two chlorophyll meters, SPAD-502 and MC-100, and two active canopy reflectance sensors, GreenSeeker handheld and Crop Circle ACS-470. Three treatments (N1, deficient, N2, conventional, and N3, excessive N application) of N concentration in the nutrient solution were applied by fertigation throughout a sweet pepper crop grown in soil in a greenhouse. Time of day of 12:00 and 15:00 h had an effect on measurements made with the SPAD-502, but only in the N1 treatment, suggesting that the effects of time of day were related to crop N status. This effect was slight, being 1.7 ± 0.02 SPAD units lower at 12:00 and 15:00 h compared to at 9:00 h (relative decrease of 3.6%). For the MC-100, a slight increase in Chlorophyll Content Index (CCI) values of 3.3 ± 0.1 units (relative increase of 6.3%) was observed at 15:00 and 18:00 h, relative to CCI values at 9:00 h, regardless of N treatment. The time of day effect on chlorophyll meters appears to be negligible in relation to the wide range of values measured in greenhouse-grown sweet pepper. Normalized Difference Vegetation Index, measured both with the GreenSeeker and Crop Circle, and Green Normalized Difference Vegetation Index, measured with the Crop Circle, were not affected by time of day in any of the N treatments, showing that these sensors and indices can be used with confidence at any time of the day.
Why it matches plant phenotyping methods植物のN状態を測定するクロロフィルメーターおよびキャノピー反射センサーについて、時刻による測定変動と使用信頼性を評価しており、センサー測定法の技術的検証が中心である。
abstractOptical sensors are a promising approach for assessing nitrogen (N) status of vegetable crops.
ABSTRACT The improvement of crop productivity under abiotic stress is one of the biggest challenges faced by the agricultural scientific community. Despite extensive research, the research-to-commercial transfer rate of abiotic stress-resistant crops remains very low. This is mainly due to the complexity of genotype◻×◻environment interactions and in particular, the ability to quantify the dynamic plant physiological response profile to a dynamic environment. Most existing phenotyping facilities collect information using robotics and automated image acquisition and analysis. However, their ability to directly measure the physiological properties of the whole plant is limited. We demonstrate a high-throughput functional phenotyping system (HFPS) that enables comparing plants’ dynamic responses to different ambient conditions in dynamic environments due to its direct and simultaneous measurement of yield-related physiological traits of plants under several treatments. The system is designed as one-to-one (1:1) plant–[sensors+controller] units, i.e., each individual plant has its own personalized sensor, controller and irrigation valves that enable (i) monitoring water-relation kinetics of each plant–environment response throughout the plant’s life cycle with high spatiotemporal resolution, (ii) a truly randomized experimental design due to multiple independent treatment scenarios for every plant, and (iii) reduction of artificial ambient perturbations due to the immobility of the plants or other objects. In addition, we propose two new resilience-quantifying-related traits that can also be phenotyped using the HFPS: transpiration recovery rate and night water reabsorption. We use the HFPS to screen the effects of two commercial biostimulants (a seaweed extract—ICL-SW, and a metabolite formula—ICL-NewFo1) on Capsicum annuum under different irrigation regimes. Biostimulants are considered an alternative approach to improving crop productivity. However, their complex mode of action necessitates cost-effective pre-field phenotyping. The combination of two types of treatment (biostimulants and drought) enabled us to evaluate the precision and resolution of the system in investigating the effect of biostimulants on drought tolerance. We analyze and discuss plant behavior at different stages, and assess the penalty and trade-off between productivity and survivability. In this test case, we suggest a protocol for the screening of biostimulants’ physiological mechanisms of action.
Why it matches plant phenotyping methods植物の生理形質を高スループットかつ個体別に測定するセンサー・制御システムを開発し、精度・解像度を評価しているため、スクリーニング応用を含む方法中心の研究です。
abstractWe demonstrate a high-throughput functional phenotyping system (HFPS)
In the past years, the diversity of Capsicum has been mainly investigated through genetics and genomics approaches, fewer efforts have been made in the field of plant phenomics. Assessment of crop traits with high-throughput methodologies could enhance the knowledge of the plant phenome, giving at the same time a key contribution to the understanding of the function of many genes. In this study, a wide germplasm collection of 307 accessions retrieved from 48 world regions, and belonging to nine Capsicum species was characterized for 54 plant, leaf, flower and fruit traits. Conventional descriptors and semi-automated tools based on image analysis and colour coordinate detection were used. Significant differences were found among accessions, between species and between sweet and spicy cultivated types, revealing a large diversity. The results highlighted how the domestication process and the continued selection have increased the variability of fruit shape and colour. Hierarchical clustering based on conventional and fruit morphological descriptors reflected the separation of species on the basis of their phylogenetic relationships. These observations suggested that the flow between distinct gene pools could have contributed to determine the similarity of the species on the basis of morphological plant and fruit parameters. The approach used represents the first high-throughput phenotyping effort in Capsicum spp. aimed at broadening the knowledge of the diversity of domesticated and wild peppers. The data could help to select best the candidates for breeding and provide new insight into the understanding of the genetic base of the fruit shape of pepper.
Why it matches plant phenotyping methods大規模な植物表現型解析を主題とし、半自動画像解析・色座標検出ツールを用いて植物、葉、花、果実の形質を高スループットに取得しているため、方法の実質的適用に該当する。
abstractAssessment of crop traits with high-throughput methodologies could enhance the knowledge of the plant phenome
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Supplement · publicSupplementary Materials
The following are available online at http://www.mdpi.com/2223-7747/7/4/103/s1 , Figure S1: Distribution of fruit traits in the 307 pepper genotypes under study, Figure S2a: Loading plot of the first and second component based on eight highly correlated fruit traits in all species under study, Figure S2b: Loading plot of the first and second component based on eight highly fruit correlated traits in domesticated and wild species, Figure S3: Hierarchical clustering based on eight highly correlated fruit traits and two most significant plant traits, Table S1: Mean, range, significance of the means within each species and among the 9 Capsicum species for plant traits (BOpen asset ↗lines:943-957Plant phenotyping relevance match · UnverifiedEurope PMC · checked 10 Sept 2026
Pepper / chilliLiDAR / point cloudFruitPose / keypoint estimation
The space pose of fruits is necessary for accurate detachment in automatic harvesting. This study presents a novel pose estimation method for sweet pepper detachment. In this method, the normal to the local plane at each point in the sweet-pepper point cloud was first calculated. The point cloud was separated by a number of candidate planes, and the scores of each plane were then separately calculated using the scoring strategy. The plane with the lowest score was selected as the symmetry plane of the point cloud. The symmetry axis could be finally calculated from the selected symmetry plane, and the pose of sweet pepper in the space was obtained using the symmetry axis. The performance of the proposed method was evaluated by simulated and sweet-pepper cloud dataset tests. In the simulated test, the average angle error between the calculated symmetry and real axes was approximately 6.5°. In the sweet-pepper cloud dataset test, the average error was approximately 7.4° when the peduncle was removed. When the peduncle of sweet pepper was complete, the average error was approximately 6.9°. These results suggested that the proposed method was suitable for pose estimation of sweet peppers and could be adjusted for use with other fruits and vegetables.
Why it matches plant phenotyping methods甜ピーマン果実の三次元姿勢を点群から推定する手法を開発し、シミュレーションおよび実データで誤差検証している。収穫対象の単なる検出ではなく、果実器官の姿勢という再利用可能な形態状態を定量化しているため、中心的なフェノタイピング手法に該当する。
abstractThis study presents a novel pose estimation method for sweet pepper detachment.
The non-destructive classification of plant materials using optical inspection techniques has been gaining much recent attention in the field of agriculture research. Among them, a near-infrared (NIR) imaging method called optical coherence tomography (OCT) has become a well-known agricultural inspection tool since the last decade. Here we investigated the non-destructive identification capability of OCT to classify diversely stained (with various staining agents) Capsicum annuum seed specimens of different cultivars. A swept source (SS-OCT) system with a spectral band of 1310 nm was used to image unstained control C. annuum seeds along with diversely stained Capsicum seeds, belonging to different cultivar varieties, such as C. annuum cv. PR Ppareum, C. annuum cv. PR Yeol, and C. annuum cv. Asia Jeombo. The obtained cross-sectional images were further analyzed for the changes in the intensity of back-scattered light (resulting due to dye pigment material and internal morphological variations) using a depth scan profiling technique to identify the difference among each seed category. The graphically acquired depth scan profiling results revealed that the control specimens exhibit less back-scattered light intensity in depth scan profiles when compared to the stained seed specimens. Furthermore, a significant back-scattered light intensity difference among each different cultivar group can be identified as well. Thus, the potential capability of OCT based depth scan profiling technique for non-destructive classification of diversely stained C. annum seed specimens of different cultivars can be sufficiently confirmed through the proposed scheme. Hence, when compared to conventional seed sorting techniques, OCT can offer multipurpose advantages by performing sorting of seeds in respective to the dye staining and provides internal structural images non-destructively.
Why it matches plant phenotyping methodsOCTによる種子の非破壊イメージングと深さ方向の散乱光プロファイリングを開発・検証し、品種および内部形態・染色状態を分類する方法が研究の中心である。
abstractthe potential capability of OCT based depth scan profiling technique for non-destructive classification of diversely stained C. annum seed specimens of different cultivars can be sufficiently confirmed through the proposed scheme.
The measurement of xylem sap flow is essential to understanding plant physiology in agriculture. Advanced hydroponics, for instance, would require sap flow measurement to observe the plant reaction to environmental variables, such as sunlight, humidity, and soil water content. However, most conventional approaches for sap flow measurement have been limited to large woody plants. Plants grown in hydroponics, e.g., tomatoes and bell peppers, are smaller and softer, and can hardly survive the invasion of thick thermal probes for flow speed measurement. This report presents a microneedle thermal probe that can be implanted into a small plant for the measurement of sap flow through the xylem. A microscale single hot wire on a single probe is used to benefit from small-scale physics in a simple configuration. The single probe enables minimally invasive measurement with a small thermal impact on plant tissues. We show that the Granier method can be modified to use the single hot wire as a heater and a temperature sensor simultaneously. Tests with a tomato stem result in a universal calibration model that can be applied to the same species. We demonstrate routine measurements of sap flow in a greenhouse tomato tree over a month, opening up the possibility for production scale application.
Why it matches plant phenotyping methods小型植物の木部樹液流を測定するマイクロニードル熱プローブを開発し、トマト茎で校正・検証しており、植物生理状態の取得手法が研究の中心である。
abstractThis report presents a microneedle thermal probe that can be implanted into a small plant for the measurement of sap flow through the xylem.
Volatile organic compounds (VOCs) have been proven to be important biomarkers for predicting pathogen/pest-induced diseases in plants 1 . Methyl salicylate (MeSA) has been identified as one such important biomarker for biotic stress in plants 2-4 . Advanced detection of MeSA could help in early identification of plant diseases and has a profound significance for precision agriculture industry to maintain effective use for disease precautions. Previous research has demonstrated the development of biosensor consisting salicylate hydroxylase (SH) / tyrosinase (TYR) for salicylate detection. However, the method requires high temperature hydrolysis and pH neutralization steps before detection, rendering it more complex for device miniaturization 5 . In this project, we aim to eliminate these additional steps by developing a tri-enzyme detection platform consisting of esterase (ES), SH and TYR for direct MeSA detection without additional hydrolysis or pH neutralization steps. Two different immobilization strategies were used and compared using a lab-on-chip model and the sensitivity and specificity were determined to be 3.1 ± 0.2 µA·cm -2 ·µM -1 and 0.8 ± 0.2 µM respectively. An open source computer hardware and software Arduino was used for fabricating the computer-controlled automatic collection device for VOC collection. The prototype of a portable device for MeSA detection device was designed and fabricated, and the measurement was carried with the enzymatic biosensor strip. Reference (1) Laothawornkitkul, J., et al., Discrimination of plant volatile signatures by an electronic nose: a potential technology for plant pest and disease monitoring. Environmental Science & Technology, 2008. 42 (22): p. 8433-8439. (2) Buttery, R., et al., Characterization of some volatile constituents of bell peppers. Journal of Agricultural and Food Chemistry, 1969. 17 (6): p. 1322-1327. (3) Seskar, M., V. Shulaev, and I. Raskin, Endogenous methyl salicylate in pathogen-inoculated tobacco plants. Plant Physiology, 1998. 116 (1): p. 387-392. (4) Zhu, J. and K.-C. Park, Methyl salicylate, a soybean aphid-induced plant volatile attractive to the predator Coccinella septempunctata. Journal of chemical ecology, 2005. 31 (8): p. 1733-1746. (5) Fang, Y., et al., Detection of methyl salicylate using bi-enzyme electrochemical sensor consisting salicylate hydroxylase and tyrosinase. Biosensors and Bioelectronics, 2016. 85 : p. 603-610.
Why it matches plant phenotyping methods植物の病害関連揮発性物質MeSAを直接検出する携帯型センサーと自動VOC収集装置の開発が中心であり、植物の病害状態に関連する生理形質の取得法に該当する。
abstractwe aim to eliminate these additional steps by developing a tri-enzyme detection platform consisting of esterase (ES), SH and TYR for direct MeSA detection without additional hydrolysis or pH neutralization steps.
Plant phenotyping relevance match · UnverifiedCrossref · OpenAlex · Europe PMC · checked 10 Sept 2026
Background and aims Global agriculture is facing the challenge of a phenotyping bottleneck due to large-scale screening/breeding experiments with improved breeds. Phenotypic analysis with high-throughput, high-accuracy and low-cost technologies has therefore become urgent. Recent advances in image-based 3D reconstruction offer the opportunity of high-throughput phenotyping. The main aim of this study was to quantify and evaluate the canopy structure of plant populations in two and three dimensions based on the multi-view stereo (MVS) approach, and to monitor plant growth and development from seedling stage to fruiting stage. Methods Multi-view images of flat-leaf cucumber, small-leaf pepper and curly-leaf eggplant were obtained by moving a camera around the plant canopy. Three-dimensional point clouds were reconstructed from images based on the MVS approach and were then converted into surfaces with triangular facets. Phenotypic parameters, including leaf length, leaf width, leaf area, plant height and maximum canopy width, were calculated from reconstructed surfaces. Accurate evaluation in 2D and 3D for individual leaves was performed by comparing reconstructed phenotypic parameters with referenced values and by calculating the Hausdorff distance, i.e. the mean distance between two surfaces. Key results Our analysis demonstrates that there were good agreements in leaf parameters between referenced and estimated values. A high level of overlap was also found between surfaces of image-based reconstructions and laser scanning. Accuracy of 3D reconstruction of curly-leaf plants was relatively lower than that of flat-leaf plants. Plant height of three plants and maximum canopy width of cucumber and pepper showed an increasing trend during the 70 d after transplanting. Maximum canopy width of eggplants reached its peak at the 40th day after transplanting. The larger leaf phenotypic parameters of cucumber were mostly found at the middle-upper leaf position. Conclusions High-accuracy 3D evaluation of reconstruction quality indicated that dynamic capture of the 3D canopy based on the MVS approach can be potentially used in 3D phenotyping for applications in breeding and field management.
Why it matches plant phenotyping methodsMVS画像から3Dキャノピーを再構成し、葉形質や草冠構造を定量化・検証する手法が研究の中心である。
abstractThe main aim of this study was to quantify and evaluate the canopy structure of plant populations in two and three dimensions based on the multi-view stereo (MVS) approach
This paper provides synthesis methods for large-scale semantic image segmentation datasets of agricultural scenes with the objective to bridge the gap between state-of-the art computer vision performance and that of computer vision in the agricultural robotics domain. We propose a novel methodology to generate renders of random meshes of plants based on empirical measurements, including the automated generation per-pixel class and depth labels for multiple plant parts. A running example is given of Capsicum annuum (sweet or bell pepper) in a high-tech greenhouse. A synthetic dataset of 10,500 images was rendered through Blender, using scenes with 42 procedurally generated plant models with randomised plant parameters. These parameters were based on 21 empirically measured plant properties at 115 positions on 15 plant stems. Fruit models were obtained by 3D scanning and plant part textures were gathered photographically. As reference dataset for modelling and evaluate segmentation performance, 750 empirical images of 50 plants were collected in a greenhouse from multiple angles and distances using image acquisition hardware of a sweet pepper harvest robot prototype. We hypothesised high similarity between synthetic images and empirical images, which we showed by analysing and comparing both sets qualitatively and quantitatively. The sets and models are publicly released with the intention to allow performance comparisons between agricultural computer vision methods, to obtain feedback for modelling improvements and to gain further validations on usability of synthetic bootstrapping and empirical fine-tuning. Finally, we provide a brief perspective on our hypothesis that related synthetic dataset bootstrapping and empirical fine-tuning can be used for improved learning.
Why it matches plant phenotyping methods植物部位のセマンティックセグメンテーション用の合成・実画像データセットと生成手法を開発し、性能比較・検証可能な形で公開しており、植物画像から部位を抽出する方法が中心である。
abstractWe propose a novel methodology to generate renders of random meshes of plants based on empirical measurements, including the automated generation per-pixel class and depth labels for multiple plant parts.
Reproduction assets foundThe paper publicly releases its synthetic and empirical Capsicum annuum image datasets (with annotations) via a 4TU/Centre DOI, explicitly stated in the conclusion.Dataset · publicur experiments. Segmentation results
show a promising next step for semantic part localisation in agriculture.
Future efforts should be aimed in further optimising the network ar-
chitectures, focussing on the performance of the infrequent classes. The
datasets and their source material are publicly released and can be
found at: https://doi.org/10.4121/uuid:884958f5-b868-46e1-b3d8-a0b5d91b02c0
Acknowledgements
This research was partially funded by the European Commission in
the Horizon2020 Programme (SWEEPER GA No. 644313). The authors
would like to thank prof.dr. R. D. Howe and dr. D. Perrin for their input
of this research and making computing resources available. The authors
declare that tOpen asset ↗10.4121/uuid:884958f5-b868-46e1-b3d8-a0b5d91b02c0pdf-raw-page:12 lines:81-119Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
We demonstrate that optical coherence tomography (OCT) is a plausible optical tool for in vivo detection of plant seeds and its morphological changes during growth. To investigate the direct impact of salt stress on seed germination, the experiment was conducted using Capsicum annuum seeds that were treated with different molar concentrations of NaCl. To determine the optimal concentration for the seed growth, the seeds were monitored for nine consecutive days. In vivo two-dimensional OCT images of the treated seeds were obtained and compared with the images of seeds that were grown using sterile distilled water. The obtained results confirm the feasibility of using OCT for the proposed application. Normalized depth profile analysis was utilized to support the conclusions.
Why it matches plant phenotyping methodsOCTを用いた種子の形態変化の非破壊・生体内モニタリング手法を開発・実証しており、植物表現型の取得方法が中心である。
abstractWe demonstrate that optical coherence tomography (OCT) is a plausible optical tool for in vivo detection of plant seeds and its morphological changes during growth.
Reproduction assets foundThe paper's supplementary material (Table S1) contains the paper-specific phenotyping measurements: seed weight and embryo thickness statistics for all NaCl-treated and control seed groups across the 9-day monitoring period, publicly available at the MDPI supplementary URL. No analysis code or image datasets are statedSupplement · publicugh Advanced Production Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (No. 314031-3). Additionally, this study was also supported by the BK21 Plus project funded by the Ministry of Education, Korea (21A20131600011).
Supplementary Materials
The following are available online at http://www.mdpi.com/1424-8220/17/12/2887/s1 . Table S1, The average weight gain observed and the averaged embryo thickness values for each group, along with its standard deviation value and the maximum and minimum values of seeds in each group that was observed during the entire monitoring process.
Click here for additional data file.
Author Contributions
The experimeOpen asset ↗lines:63-81Plant phenotyping relevance match · UnverifiedEurope PMC · checked 15 Sept 2026
The spectrum of an ultrasonic return echo from plants has been shown to contain useful information. The research reported in this paper focused on developing an ultrasonic sensing system and analyzing the ultrasonic classification features that would ultimately be used as the basis for a yield estimation robotic system. An algorithm was also developed for prediction of fruit mass per plant based on the ultrasonic echo return from a plant. The ultrasonic sensor system was tested in lab and pepper greenhouse environments and on single pepper plants, single leaves and fruit. The results showed the potential of ultrasonic sensors for such a robot in classifying plants and greenhouse infrastructures such as walls. It showed the robot’s ability to detect hidden plant rows and fruits as well as making an estimation of the fruit mass in single plants. A multi-linear regression model developed for estimating the energy level was found to be highly significant with R ² of 0.64 and 0.84 for 28–32 and 20–28 kHz ranges respectively. This estimated model was used to derive a prediction method for fruit mass per plant that yielded an R² of 0.34.
Why it matches plant phenotyping methods植物からの超音波エコーを用いて果実重量を推定するセンシングシステムとアルゴリズムを開発・評価しており、植物形質の取得方法が研究の中心である。
abstractfocused on developing an ultrasonic sensing system and analyzing the ultrasonic classification features
Hyperspectral remote sensing can be applied to the rapid and nondestructive monitoring of heavy-metal pollution in crops. To realize the rapid and real-time detection of cadmium in the edible part (fruit) of Capsicum annuum, the leaf spectral reflectance of plants exposed to different levels of cadmium stress was measured using hyperspectral remote sensing during four growth stages. The spectral indices or bands sensitive to cadmium stress were determined by correlation analysis, and hyperspectral estimation models for predicting the cadmium content in the fruit of C. annuum during the mature growth stage were established. The models were cross validated by taking the sensitive spectral indices in the bud stage and the sensitive spectral bands in the flowering stage as the input variables. The results indicated that cadmium accumulated in the leaves and fruit of C. annuum and leaf cadmium content in the three early growth stages were correlated with the cadmium content of the pepper in the mature stage. Leaf spectral reflectance was sensitive to cadmium stress, and the first derivative of the original spectral reflectance was strongly correlated with leaf cadmium content during all growth stages. Among the established models, the multiple regression model based on the sensitive spectral bands in the flowering stage was optimal for predicting fruit cadmium content of the pepper. This model provides a promising method to ensure food safety during the early growth stage of the plant.
Why it matches plant phenotyping methods葉のハイパースペクトル反射からトウガラシ果実のカドミウム濃度を推定するモデルを構築し、交差検証しており、植物状態の非破壊計測手法が研究の中心である。
abstracthyperspectral estimation models for predicting the cadmium content in the fruit of C. annuum during the mature growth stage were established.
Seed germination rate differs based on chemical treatments, and nondestructive measurements of germination rate have become an essential requirement in the field of agriculture. Seed scientists and other biologists are interested in optical sensing technologies-based biological discoveries due to nondestructive detection capability. Optical coherence tomography (OCT) has recently emerged as a powerful method for biological and plant material discoveries. We report an extended application of OCT by monitoring the germination rate acceleration of chemically primed seeds. To validate the versatility of the method, Capsicum annum seeds were primed using three chemical compounds: sterile distilled water (SDW), butandiol, and 1-hexadecene. Monitoring was performed using a 1310-nm swept source OCT system. The results confirmed more rapid morphological variations in the seeds treated with 1-hexadecene medium than the seeds treated with SDW and butandiol within 8 consecutive days. In addition, fresh weight measurements (gold standard) of seeds were monitored for 15 days, and the obtained results were correlated with the OCT results. Thus, such a method can be used in various agricultural fields, and OCT shows potential as a rigorous sensing method for selecting the optimal plant growth-promoting chemical compounds rapidly, when compared with the gold standard methods.
Why it matches plant phenotyping methodsOCTを用いて種子の発芽率・形態変化を非破壊計測する方法を提示し、処理条件間の結果を重量測定と相関させて検証しており、植物表現型取得法が中心です。
abstractWe report an extended application of OCT by monitoring the germination rate acceleration of chemically primed seeds.
Crown and root rot of tomato and sweet pepper can be caused by Phytophthora parasitica. In this work, 23 P. parasitica isolates from diseased pepper or tomato plants as well as 54 isolates from 23 monocrop tomato soils (from Spain and Chile) and one from a pepper soil were studied for their host–pathogen response. Results show significant host specificity for the isolates from tomato plants and tomato soils (63 of 64 isolates were unable to cause disease in pepper). None of the pepper plant/soil isolates showed pathogenicity on tomato, and only four of 14 reproduced their pathogenicity on pepper. Only one tomato isolate was pathogenic to both Solanaceae species. Two different inoculation protocols were evaluated (substrate irrigation and stem cutting). All isolates which expressed pathogenicity when stem inoculated also did it when root inoculated, but not vice-versa. Therefore, the recommended test protocol for tomato and pepper breeding programmes is that based on root inoculation by irrigation.
Why it matches plant phenotyping methods病原性そのものの研究だが、2種類の接種法を比較評価し、トマト・ピーマンの病害表現型を再現性よく測定する育種向け試験法を推奨しており、表現型取得法の検証が中心的である。
abstractTwo different inoculation protocols were evaluated (substrate irrigation and stem cutting).
A comprehensive evaluation of the recently developed Fraunhofer line depth (FLD) and laser-induced saturation pulse (FLD-LISP) method was conducted to measure chlorophyll fluorescence (ChlF) parameters of the quantum yield of photosystem II (ΦPSII), non-photochemical quenching (NPQ), and the photosystem II-based electron transport rate (ETR) in three plant species including paprika (C3 plant), maize (C4 plant), and pachira (C3 plant). First, the relationships between photosynthetic photon flux density (PPFD) and ChlF parameters retrieved using FLD-LISP and the pulse amplitude-modulated (PAM) methods were analyzed for all three species. Then the relationships between ChlF parameters measured using FLD-LISP and PAM were evaluated for the plants in different growth stages of leaves from mature to aging conditions. The relationships of ChlF parameters/PPFD were similar in both FLD-LISP and PAM methods in all plant species. ΦPSII showed a linear relationship with PPFD in all three species whereas NPQ was found to be linearly related to PPFD in paprika and maize, but not for pachira. The ETR/PPFD relationship was nonlinear with increasing values observed for PPFDs lower than about 800 μmol m−2 s−1 for paprika, lower than about 1200 μmol m−2 s−1 for maize, and lower than about 800 μmol m−2 s−1 for pachira. The ΦPSII, NPQ, and ETR of both the FLD-LISP and PAM methods were very well correlated (R2 = 0.89, RMSE = 0.05), (R2 = 0.86, RMSE = 0.44), and (R2 = 0.88, RMSE = 24.69), respectively, for all plants. Therefore, the FLD-LISP method can be recommended as a robust technique for the estimation of ChlF parameters.
Why it matches plant phenotyping methodsFLD-LISP法による植物のクロロフィル蛍光パラメータ推定を、PAM法と複数種・生育段階で比較検証しており、植物生理形質の取得手法が研究の中心である。
abstractA comprehensive evaluation of the recently developed Fraunhofer line depth (FLD) and laser-induced saturation pulse (FLD-LISP) method was conducted to measure chlorophyll fluorescence (ChlF) parameters
Automation of disease detection and monitoring can facilitate targeted and timely disease control, which can lead to increased yield, improved crop quality and reduction in the quantity of applied pesticides. Further advantages are reduced production costs, reduced exposure to pesticides for farm workers and inspectors and increased sustainability. Symptoms are unique for each disease and crop, and each plant may suffer from multiple threats. Thus, a dedicated integrated disease-detection system and algorithms are required. The development of such a robotic detection system for two major threats of bell pepper plants: powdery mildew (PM) and Tomato spotted wilt virus (TSWV), is presented. Detection algorithms were developed based on principal component analysis using RGB and multispectral NIR-R-G sensors. High accuracy was obtained for pixel classification as diseased or healthy, for both diseases, using RGB imagery (PM: 95%, TSWV: 90%). NIR-R-G multispectral imagery yielded low classification accuracy (PM: 80%, TSWV: 61%). Accordingly, the final sensing apparatus was composed of a RGB sensor and a single-laser-beam distance sensor. A relatively fast cycle time (average 26.7 s per plant) operation cycle for detection of the two diseases was developed and tested. The cycle time was mainly influenced by sub-tasks requiring motion of the manipulator. Among these tasks, the most demanding were the determination of the required detection position and orientation. The time for task completion may be reduced by increasing the robotic work volume and by improving the algorithm for determining position and orientation.
Why it matches plant phenotyping methods植物病害の症状をRGB・マルチスペクトル画像で検出するロボット型フェノタイピングシステムとアルゴリズムの開発・評価が中心であり、植物状態の測定手法に該当する。
abstractThe development of such a robotic detection system for two major threats of bell pepper plants: powdery mildew (PM) and Tomato spotted wilt virus (TSWV), is presented.
The two-spotted spider mite (Tetranychus urticae Koch; TSSM) feeds on the under-surface of leaves, piercing the chloroplast-containing cells and affecting pigments as well as leaf structure. This damage could be spectrally detectable in the visible and near-infrared spectral regions. The aim was to spectrally explore the ability to assess TSSM damage levels in greenhouse-grown pepper (Capsicum annuum) and bean (Phaseolus vulgaris) leaves. Several vegetation indices (VIs) provided the ability to classify early TSSM damage using a one-way analysis of variance. Hyperspectral (400–1000 nm) and multispectral (five common bands) data were analysed and cross-validated independently by partial least squares-discriminant analysis models. These analyses resulted in 100% and 95% success in identifying early damage with hyperspectral data reflected from pepper and bean leaves, respectively, and in 92% with multispectral data reflected from pepper leaves. Although the TSSM activity occurred on the underside of leaves their damage can be spectrally detected by reflected data from the upper side. Early TSSM damage identification to greenhouse pepper and bean leaves, that their sole damage was by TSSM, can be obtained by VIs, hyperspectral data, and multispectral data. This study shows that by using sub leaf spatial resolution early damage by TSSM can be spectrally detected. It can be potentially applied for greenhouses as well as fields as an early detection method for TSSM management.
Why it matches plant phenotyping methods葉のスペクトル反射からハダニ被害という植物状態を検出・分類する手法を、ハイパースペクトル/マルチスペクトルデータと検証済みモデルで評価しており、表現型取得が中心です。
abstractThe aim was to spectrally explore the ability to assess TSSM damage levels in greenhouse-grown pepper (Capsicum annuum) and bean (Phaseolus vulgaris) leaves.
Image processing can proces many colors in the fruit, especially in fruit of chili. There are several colors contained in the chili fruit that is green, orange and red. Chilies are not predictable price because the chili have fluctuate prices, the chili can be determined is only the quality. The main parameters of this research are chili size and color of the chili, because these fitures can be processed to get output, namely the prediction of the chili quality. Some processes be used to predict the chili. These processes are extraction of color RGB, LAB conversion of to RGB and Grayscale, level adjustment, color Region. Some of these processes do to get a percentage of the stalk (after being separated by body of the chili) and percentage of the maturity. The last proces is classifying the chili that have been obtained percentage of stalk and maturity then using Naive Buyes Classifier metod for classifying to four kind fo quality, namely quality A, B, C and D. These qualities classification are A : consists of red chilies or ripe chili that has big size, B : consists of measuring big green chili, C : consists of small pieces of red chili and D : comprises small green chili.
Why it matches plant phenotyping methods唐辛子果実の画像からサイズ、色、成熟度、へたの割合を抽出し、品質分類する画像処理・分類ワークフローが研究の中心であり、植物器官の観測可能な形質を推定しているため。
abstractThe main parameters of this research are chili size and color of the chili, because these fitures can be processed to get output, namely the prediction of the chili quality.
This paper presents a novel pose estimation algorithm for stem position detection in Japanese green pepper automatic harvesting. When the available visual cues do not provide sufficient information to the harvesting robot, information about the pose of the fruit in space is necessary for accurate stem position detection. In the proposed method the orientation of a fruit in space is obtained by fitting a model to surface points of the fruit. These surface points are acquired using a Lidar type laser range finder, and the point matching is performed using a coherent point drift algorithm with two model transformation methods, rigid and affine. The performance of the proposed method was evaluated both under laboratory conditions and in a greenhouse. In the laboratory test, the mean total error for the affine transformation was less than 25mm in 42 of 49 positions, less than 20mm in 28 of 49 positions and less than 15mm in 19 of 49 positions. For the rigid transformation, the same error was less than 25mm in 39 of 49 positions, less than 20mm in 31 of 49 positions and less than 15mm in 11 of 49 positions. The total error of the affine transformation was found to be proportional to the inclination angle, as the mean error was 11mm, 15mm, and 23mm for inclination angles of 15, 30 and 45 degrees, respectively. No relationship was found between the mean total error and the inclination angle for the rigid transformation, as the calculated mean total error was 20mm, 18mm, and 20mm for inclination angles of 15, 30 and 45 degrees, respectively. In the greenhouse test, the stem was calculated to be within the cutting range for 81 of 107 instances for affine transformation and for 66 of 107 for rigid transformation. These results suggest that the proposed method is suitable for stem position detection in the automatic harvesting of green pepper, and could be adjusted for use with other fruits and vegetables.
Why it matches plant phenotyping methodsLiDAR点群とモデル適合により果実の3D姿勢を推定する手法を開発し、実験室および温室で誤差評価している。収穫ロボット用途だが、果実の向きという再利用可能な器官形質を定量化する技術が中心である。
abstractThis paper presents a novel pose estimation algorithm for stem position detection in Japanese green pepper automatic harvesting.
Three-dimensional (3D) reconstruction of a tree canopy is an important step in order to measure canopy geometry, such as height, width, volume, and leaf cover area. In this research, binocular stereo vision was used to recover the 3D information of the canopy. Multiple images were taken from different views around the target. The Structure-from-motion (SfM) method was employed to recover the camera calibration matrix for each image, and the corresponding 3D coordinates of the feature points were calculated and used to recover the camera calibration matrix. Through this method, a sparse projective reconstruction of the target was realized. Subsequently, a ball pivoting algorithm was used to do surface modeling to realize dense reconstruction. Finally, this dense reconstruction was transformed to metric reconstruction through ground truth points which were obtained from camera calibration of binocular stereo cameras. Four experiments were completed, one for a known geometric box, and the other three were: a croton plant with big leaves and salient features, a jalapeno pepper plant with median leaves, and a lemon tree with small leaves. A whole-view reconstruction of each target was realized. The comparison of the reconstructed box’s size with the real box’s size shows that the 3D reconstruction is in metric reconstruction.
Why it matches plant phenotyping methods植物キャノピーの3D再構成手法を開発・検証し、高さ、幅、体積、葉面積などの形態形質の計測を目的としているため、植物フェノタイピング手法が中心である。
abstractThree-dimensional (3D) reconstruction of a tree canopy is an important step in order to measure canopy geometry, such as height, width, volume, and leaf cover area.
Canopy photosynthesis has typically been estimated using mathematical models that have the following assumptions: the light interception inside the canopy exponentially declines with the canopy depth, and the photosynthetic capacity is affected by light interception as a result of acclimation. However, in actual situations, light interception in the canopy is quite heterogenous depending on environmental factors such as the location, microclimate, leaf area index, and canopy architecture. It is important to apply these factors in an analysis. The objective of the current study is to estimate the canopy photosynthesis of paprika (Capsicum annuum L.) with an analysis of by simulating the intercepted irradiation of the canopy using a 3D ray-tracing and photosynthetic capacity in each layer. By inputting the structural data of an actual plant, the 3D architecture of paprika was reconstructed using graphic software (Houdini FX, FX, Canada). The light curves and A/C i curve of each layer were measured to parameterize the Farquhar, von Caemmerer, and Berry (FvCB) model. The difference in photosynthetic capacity within the canopy was observed. With the intercepted irradiation data and photosynthetic parameters of each layer, the values of an entire plant's photosynthesis rate were estimated by integrating the calculated photosynthesis rate at each layer. The estimated photosynthesis rate of an entire plant showed good agreement with the measured plant using a closed chamber for validation. From the results, this method was considered as a reliable tool to predict canopy photosynthesis using light interception, and can be extended to analyze the canopy photosynthesis in actual greenhouse conditions.
Why it matches plant phenotyping methods3D植物構造の再構築と光線追跡を用いて個体・群落光合成を推定する方法を開発し、閉鎖チャンバー測定で妥当性を検証しており、表現型取得・推定手法が研究の中心である。
abstractThe objective of the current study is to estimate the canopy photosynthesis of paprika (Capsicum annuum L.) with an analysis of by simulating the intercepted irradiation of the canopy using a 3D ray-tracing and photosynthetic capacity in each layer.
BACKGROUND: The characterization of internal (°Brix, pH, malic acid, total phenolic compounds, ascorbic acid and total carotenoid content) and external (color, firmness and pericarp wall thickness) pepper quality is necessary to better understand its possible applications and increase consumer awareness of its benefits. The main aim of this work was to examine the feasibility of using visible/near‐infrared reflectance spectroscopy (VIS‐NIRS) to predict quality parameters in different pepper types. Commercially available spectrophotometers were evaluated for this purpose: a Polychromix Phazir spectrometer for intact raw pepper, and a scanning monochromator for freeze‐dried pepper. RESULTS: The RPD values (ratio of the standard deviation of the reference data to the standard error of prediction) obtained from the external validation exceeded a value of 3 for chlorophyll a and total carotenoid content; values ranging between 2.5 < RPD < 3 for total phenolic compounds; between 1.5 < RPD <2.5 for °Brix, pH, color parameters a* and h* and chlorophyll b; and RPD values below 1.5 for fruit firmness, pericarp wall thickness, color parameters C*, b* and L*, vitamin C and malic acid content. CONCLUSION: The present work has led to the development of multi‐type calibrations for pepper quality parameters in intact and freeze‐dried peppers. The majority of NIRS equations obtained were suitable for screening purposes in pepper breeding programs. Components such as pigments (xanthophyll, carotenes and chlorophyll), glucides, lipids, cellulose and water were used by modified partial least‐squares regression for modeling the predicting equations.
Why it matches plant phenotyping methodsVIS/NIR分光法によるピーマンの内部・外部形質推定モデルの開発と外部検証が研究の中心であり、育種向けスクリーニングへの適用も示している。
abstractThe main aim of this work was to examine the feasibility of using visible/near‐infrared reflectance spectroscopy (VIS‐NIRS) to predict quality parameters in different pepper types.
Image processing can proces many colors in the fruit, especially in fruit of chili. There are several colors contained in the chili fruit that is green, orange and red. Chilies are not predictable price because the chili have fluctuate prices, the chili can be determined is only the quality. The main parameters of this research are chili size and color of the chili, because these fitures can be processed to get output, namely the prediction of the chili quality. Some processes be used to predict the chili. These processes are extraction of color RGB, LAB conversion of to RGB and Grayscale, level adjustment, color Region. Some of these processes do to get a percentage of the stalk (after being separated by body of the chili) and percentage of the maturity. The last proces is classifying the chili that have been obtained percentage of stalk and maturity then using Naive Buyes Classifier metod for classifying to four kind fo quality, namely quality A, B, C and D. These qualities classification are A : consists of red chilies or ripe chili that has big size, B : consists of measuring big green chili, C : consists of small pieces of red chili and D : comprises small green chili.
Why it matches plant phenotyping methods唐辛子果実の画像から色・サイズ・成熟度・果柄割合を抽出し、Naive Bayesで品質分類する画像解析手法が研究の中心であり、植物器官の観測可能な形質を推定している。
abstractThe main parameters of this research are chili size and color of the chili, because these fitures can be processed to get output, namely the prediction of the chili quality.
For plant breeding and growth monitoring, accurate measurements of plant structure parameters are very crucial. We have, therefore, developed a high efficiency Multi-Camera Photography (MCP) system combining Multi-View Stereovision (MVS) with the Structure from Motion (SfM) algorithm. In this paper, we measured six variables of nursery paprika plants and investigated the accuracy of 3D models reconstructed from photos taken by four lens types at four different positions. The results demonstrated that error between the estimated and measured values was small, and the root-mean-square errors (RMSE) for leaf width/length and stem height/diameter were 1.65 mm (R² = 0.98) and 0.57 mm (R² = 0.99), respectively. The accuracies of the 3D model reconstruction of leaf and stem by a 28-mm lens at the first and third camera positions were the highest, and the number of reconstructed fine-scale 3D model shape surfaces of leaf and stem is the most. The results confirmed the practicability of our new method for the reconstruction of fine-scale plant model and accurate estimation of the plant parameters. They also displayed that our system is a good system for capturing high-resolution 3D images of nursery plants with high efficiency.
Why it matches plant phenotyping methods幼苗パプリカの葉・茎形状を推定するマルチカメラ3D計測システムを開発し、実測値との精度検証を行っており、植物表現型取得手法が研究の中心である。
abstractThe results demonstrated that error between the estimated and measured values was small, and the root-mean-square errors (RMSE) for leaf width/length and stem height/diameter were 1.65 mm (R² = 0.98) and 0.57 mm (R² = 0.99), respectively.
Plant phenotyping relevance match · UnverifiedCrossref · Europe PMC · checked 15 Sept 2026
Most agricultural robots, fruit harvesting systems in particular, use computer vision to detect their fruit targets. Exploiting the uniqueness of fruit colour amidst the foliage, almost all of these computer vision systems rely on colour features to identify the fruit in the image. However, often the colour of fruit cannot be discriminated from its background, especially under unstable illumination conditions, thus rendering the detection and segmentation of the target highly sensitive or unfeasible in colour space. While multispectral signals, especially those outside the visible spectrum, may alleviate this difficulty, simpler, cheaper, and more accessible solutions are desired. Here exploiting both RGB and range data to analyse shape-related features of objects both in the image plane and 3D space is proposed. In particular, 3D surface normal features, 3D plane-reflective symmetry, and image plane highlights from elliptic surface points are combined to provide shape-based detection of fruits in 3D space regardless of their colour. Results are shown using a particularly challenging sweet pepper dataset with a significant degree of occlusions.
Why it matches plant phenotyping methods果実の色に依存しないRGB・距離データによる3D形状ベースの果実検出手法を開発・評価しており、ロボット収穫対象の単なる位置特定を超えない点はあるものの、植物器官(果実)の画像取得・抽出法が中心的な技術貢献である。
abstractHere exploiting both RGB and range data to analyse shape-related features of objects both in the image plane and 3D space is proposed.